BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7063

Title: Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72.

Deposition date: 2006-04-10 Original release date: 2007-02-01

Authors: Aramini, J.; Swapna, G.; Ramelot, T.; Ho, C.; Cunningham, K.; Ma, L.-C.; Shetty, K.; Xiao, R.; Acton, T.; Kennedy, M.; Montelione, G.

Citation: Aramini, J.; Swapna, G.; Ramelot, T.; Ho, C.; Cunningham, K.; Ma, L.-C.; Shetty, K.; Xiao, R.; Acton, T.; Kennedy, M.; Montelione, G.. "Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72."  . ., .-..

Assembly members:
putative cytoplasmic protein, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 602   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
putative cytoplasmic protein: MYLRPDEVARVLEKAGFTVD VVTNKTYGYRRGENYVYVNR EARMGRTALIIHPRLKDRSS SLADPASDIKTCDHYQNFPL YLGGETHEHYGIPHGFSSRI ALERYLNGLFGDEKTDLEHH HHHH

Data sets:
Data typeCount
13C chemical shifts538
15N chemical shifts126
1H chemical shifts831
coupling constants65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1putative cytoplasmic protein1

Entities:

Entity 1, putative cytoplasmic protein 124 residues - Formula weight is not available

1   METTYRLEUARGPROASPGLUVALALAARG
2   VALLEUGLULYSALAGLYPHETHRVALASP
3   VALVALTHRASNLYSTHRTYRGLYTYRARG
4   ARGGLYGLUASNTYRVALTYRVALASNARG
5   GLUALAARGMETGLYARGTHRALALEUILE
6   ILEHISPROARGLEULYSASPARGSERSER
7   SERLEUALAASPPROALASERASPILELYS
8   THRCYSASPHISTYRGLNASNPHEPROLEU
9   TYRLEUGLYGLYGLUTHRHISGLUHISTYR
10   GLYILEPROHISGLYPHESERSERARGILE
11   ALALEUGLUARGTYRLEUASNGLYLEUPHE
12   GLYASPGLULYSTHRASPLEUGLUHISHIS
13   HISHISHISHIS

Samples:

sample_1: putative cytoplasmic protein, [U-13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_2: putative cytoplasmic protein, [U-13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 100%; H2O 95%

sample_3: putative cytoplasmic protein, [U-5% 13C; U-15N], 1.07 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
4D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
high resolution CH-HSQC (stereospecific assignment of Val/Leu methyls)not availablenot availablenot available
backbone TR exptsnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D TOCSYsnot availablenot availablenot available
aromatic exptsnot availablenot availablenot available

Software:

xwinnmr v3.5pl6 - collection

VNMR v6.1C - collection

SPARKY v3.110 - data analysis

AutoAssign v1.17.0 - data analysis

AutoStruct v2.1.1 - refinement

X-PLOR NIH v2.11.2 - refinement

CNS v1.1 - refinement

PDBStat v4.01 - data analysis

PSVS v1.2 - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAJ37798 BAP08706
EMBL CAD05911 CAR34225 CAR38517 CBG25775 CBW18883
GB AAL21686 AAO70265 AAX66640 ABX68827 ACF63119
PIR AH0840
REF NP_457200 NP_461727 NP_806405 WP_000281302 WP_000281303

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts