BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7070

Title: RRMs 1 and 2 of Prp24 from S. cerevisiae   PubMed: 17131032

Deposition date: 2006-04-12 Original release date: 2006-12-04

Authors: Reiter, Nicholas; Lee, Donghan; Wang, Yun-Xin; Tonelli, Marco; Bahrami, Arash; Cornilescu, Claudia; Butcher, Samuel

Citation: Reiter, Nicholas; Lee, Donghan; Wang, Yun-Xing; Tonelli, Marco; Bahrami, Arash; Cornilescu, Claudia; Butcher, Samuel. "Resonance assignments for the two N-terminal RNA recognition motifs (RRM) of the S. cerevisiae Pre-mRNA Processing Protein Prp24"  J. Biomol. NMR 36, 58-58 (2006).

Assembly members:
PRP24 (RRM 1 and 2), polymer, 161 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Metazoa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PRP24 (RRM 1 and 2): MRELTTVLVKNLPKSYNQNK VYKYFKHCGPIIHVDVADSL KKNFRFARIEFARYDGALAA ITKTHKVVGQNEIIVSHLTE CTLWMTNFPPSYTQRNIRDL LQDINVVALSIRLPSLRFNT SRRFAYIDVTSKEDARYCVE KLNGLKIEGYTLVTKVSNPL E

Data sets:
Data typeCount
13C chemical shifts687
15N chemical shifts163
1H chemical shifts1108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer (2 RRM domains)1

Entities:

Entity 1, monomer (2 RRM domains) 161 residues - Formula weight is not available

1   METARGGLULEUTHRTHRVALLEUVALLYS
2   ASNLEUPROLYSSERTYRASNGLNASNLYS
3   VALTYRLYSTYRPHELYSHISCYSGLYPRO
4   ILEILEHISVALASPVALALAASPSERLEU
5   LYSLYSASNPHEARGPHEALAARGILEGLU
6   PHEALAARGTYRASPGLYALALEUALAALA
7   ILETHRLYSTHRHISLYSVALVALGLYGLN
8   ASNGLUILEILEVALSERHISLEUTHRGLU
9   CYSTHRLEUTRPMETTHRASNPHEPROPRO
10   SERTYRTHRGLNARGASNILEARGASPLEU
11   LEUGLNASPILEASNVALVALALALEUSER
12   ILEARGLEUPROSERLEUARGPHEASNTHR
13   SERARGARGPHEALATYRILEASPVALTHR
14   SERLYSGLUASPALAARGTYRCYSVALGLU
15   LYSLEUASNGLYLEULYSILEGLUGLYTYR
16   THRLEUVALTHRLYSVALSERASNPROLEU
17   GLU

Samples:

sample_1: PRP24 (RRM 1 and 2), [U-15N; U-13C], 0.6 ± 0.3 mM; K(PO)4) 50 mM; KCL 0.1 M; DTT 2 mM

conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-_aliphpatic_NOESYsample_1not availableconditions_1
3D_15N-_NOESYsample_1not availableconditions_1
3D_13C-aromatic_NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 16230 16243 16244 16246
PDB
DBJ GAA25719
EMBL CAA89251 CAY82100
GB AAU09775 AHY76723 EDN64207 EDV11758 EDZ69961
REF NP_013995
SP P49960
TPG DAA10168

Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone or all simulated shifts