BMRB Entry 7105
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7105
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Title: 1H, 13C and 15N chemical shift assignments for SRY.B in complex with 16-mer DNA PubMed: 16813837
Deposition date: 2006-05-09 Original release date: 2006-08-07
Authors: Stott, Katherine; Tang, George; Lee, Keng-Boon; Thomas, Jean
Citation: Stott, Katherine; Tang, George; Lee, Keng-Boon; Thomas, Jean. "Structure of a Complex of Tandem HMG Boxes and DNA" J. Mol. Biol. 360, 90-104 (2006).
Assembly members:
SRY.B, polymer, 159 residues, Formula weight is not available
16-mer DNA trand 1, polymer, 16 residues, Formula weight is not available
16-mer DNA strand 2, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SRY.B: VQDRVKRPMNAFIVWSRDQR
RKMALENPRMRNSEISKQLG
YQWKMLTEAEKWPFFQEAQK
LQAMHREKYPNYKYRKGETK
KKFKDPNAPKRPPSAFFLFC
SEYRPKIKGEHPGLSIGDVA
KKLGEMWNNTAADDKQPYEK
KAAKLKEKYEKDIAAYRAK
16-mer DNA trand 1: GGGATCTAAACAATGC
16-mer DNA strand 2: GCATTGTTTAGATCCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 619 |
| 15N chemical shifts | 163 |
| 1H chemical shifts | 1493 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SRY.B in complex with 16-mer DNA | 1 |
| 2 | SRY.B in complex with 16-mer DNA strand 1 | 2 |
| 3 | SRY.B in complex with 16-mer DNA strand 2 | 3 |
Entities:
Entity 1, SRY.B in complex with 16-mer DNA 159 residues - Formula weight is not available
| 1 | VAL | GLN | ASP | ARG | VAL | LYS | ARG | PRO | MET | ASN | ||||
| 2 | ALA | PHE | ILE | VAL | TRP | SER | ARG | ASP | GLN | ARG | ||||
| 3 | ARG | LYS | MET | ALA | LEU | GLU | ASN | PRO | ARG | MET | ||||
| 4 | ARG | ASN | SER | GLU | ILE | SER | LYS | GLN | LEU | GLY | ||||
| 5 | TYR | GLN | TRP | LYS | MET | LEU | THR | GLU | ALA | GLU | ||||
| 6 | LYS | TRP | PRO | PHE | PHE | GLN | GLU | ALA | GLN | LYS | ||||
| 7 | LEU | GLN | ALA | MET | HIS | ARG | GLU | LYS | TYR | PRO | ||||
| 8 | ASN | TYR | LYS | TYR | ARG | LYS | GLY | GLU | THR | LYS | ||||
| 9 | LYS | LYS | PHE | LYS | ASP | PRO | ASN | ALA | PRO | LYS | ||||
| 10 | ARG | PRO | PRO | SER | ALA | PHE | PHE | LEU | PHE | CYS | ||||
| 11 | SER | GLU | TYR | ARG | PRO | LYS | ILE | LYS | GLY | GLU | ||||
| 12 | HIS | PRO | GLY | LEU | SER | ILE | GLY | ASP | VAL | ALA | ||||
| 13 | LYS | LYS | LEU | GLY | GLU | MET | TRP | ASN | ASN | THR | ||||
| 14 | ALA | ALA | ASP | ASP | LYS | GLN | PRO | TYR | GLU | LYS | ||||
| 15 | LYS | ALA | ALA | LYS | LEU | LYS | GLU | LYS | TYR | GLU | ||||
| 16 | LYS | ASP | ILE | ALA | ALA | TYR | ARG | ALA | LYS |
Entity 2, SRY.B in complex with 16-mer DNA strand 1 16 residues - Formula weight is not available
| 1 | DG | DG | DG | DA | DT | DC | DT | DA | DA | DA | ||||
| 2 | DC | DA | DA | DT | DG | DC |
Entity 3, SRY.B in complex with 16-mer DNA strand 2 16 residues - Formula weight is not available
| 1 | DG | DC | DA | DT | DT | DG | DT | DT | DT | DA | ||||
| 2 | DG | DA | DT | DC | DC | DC |
Samples:
sample_1: SRY.B, [U-99% 13C; U-99% 15N], 1 mM; Sodium phosphate 10 mM; EDTA 1 mM; DTT, [U-98% 2H], 2 mM
conditions_1: pH: 6.8; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D HSQC | sample_1 | not available | conditions_1 |
| 3D HN(CO)CA | sample_1 | not available | conditions_1 |
| 3D HNCA | sample_1 | not available | conditions_1 |
| 3D CBCA(CO)NH | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D(H)CC(CO)NH-TOCSY | sample_1 | not available | conditions_1 |
| 3D TOCSY-15N-HSQC | sample_1 | not available | conditions_1 |
| 3D NOESY-15N-HSQC | sample_1 | not available | conditions_1 |
| 3D HC(C)H-TOCSY | sample_1 | not available | conditions_1 |
| 3D NOESY-13C-HSQC | sample_1 | not available | conditions_1 |
| 3D HNHA | sample_1 | not available | conditions_1 |
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D DQF-COSY | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
| 2D [F1]-13C/15N-filtered TOCSY | sample_1 | not available | conditions_1 |
| 2D [F1]-13C/15N-filtered DQF COSY | sample_1 | not available | conditions_1 |
| 2D [F1]-13C/15N-filtered NOESY | sample_1 | not available | conditions_1 |
| 3D [F1]-13C/15N-filtered NOESY-13C-HSQC | sample_1 | not available | conditions_1 |
| 2D [F1,F2]-13C/15N-filtered NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG51867 |
| GB | EGW08959 |
| REF | XP_004384214 XP_004435356 XP_004680239 XP_005902227 XP_007956028 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts