BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7106

Title: NMR Assignment of human RGS18 (regulator of G-protein signalling)   PubMed: 16964532

Deposition date: 2006-05-11 Original release date: 2006-08-15

Authors: Higman, Victoria; Leidert, Martina; Elkins, Jon; Soundararajan, Meera; Brockmann, Christoph; Schmieder, Peter; Sundstrom, Michael; Arrowsmith, Cheryl; Weigelt, Johan; Edwards, Aled; Diehl, Annette; Oschkinat, Hartmut; Ball, Linda

Citation: Higman, Victoria; Leidert, Martina; Diehl, Annette; Elkins, Jonathan; Soundararajan, Meera; Oschkinat, Hartmut; Ball, Linda. "NMR assignment of Human RGS18"  J. Biomol. NMR 36, 72-72 (2006).

Assembly members:
RGS18, polymer, 151 residues, 17760 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
RGS18: SMVSPEEAVKWGESFDKLLS HRDGLEAFTRFLKTEFSEEN IEFWIACEDFKKSKGPQQIH LKAKAIYEKFIQTDAPKEVN LDFHTKEVITNSITQPTLHS FDAAQSRVYQLMEQDSYTRF LKSDIYLDLMEGRPQRPTNL RRRSRSFTCNE

Data sets:
Data typeCount
13C chemical shifts601
15N chemical shifts134
1H chemical shifts924

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RGS181

Entities:

Entity 1, RGS18 151 residues - 17760 Da.

1   SERMETVALSERPROGLUGLUALAVALLYS
2   TRPGLYGLUSERPHEASPLYSLEULEUSER
3   HISARGASPGLYLEUGLUALAPHETHRARG
4   PHELEULYSTHRGLUPHESERGLUGLUASN
5   ILEGLUPHETRPILEALACYSGLUASPPHE
6   LYSLYSSERLYSGLYPROGLNGLNILEHIS
7   LEULYSALALYSALAILETYRGLULYSPHE
8   ILEGLNTHRASPALAPROLYSGLUVALASN
9   LEUASPPHEHISTHRLYSGLUVALILETHR
10   ASNSERILETHRGLNPROTHRLEUHISSER
11   PHEASPALAALAGLNSERARGVALTYRGLN
12   LEUMETGLUGLNASPSERTYRTHRARGPHE
13   LEULYSSERASPILETYRLEUASPLEUMET
14   GLUGLYARGPROGLNARGPROTHRASNLEU
15   ARGARGARGSERARGSERPHETHRCYSASN
16   GLU

Samples:

sample_1: RGS18, [U-95% 15N], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM

sample_2: RGS18, [U-95% 15N; U-95% 13C], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM

sample_3: RGS18, [U-95% 15N; U-95% 13C], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM; D2O 100%

conditions_1: pH: 6.0; temperature: 297.0 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
3D HNCOsample_2not availableconditions_1
3D CBCA(CO)NNHsample_2not availableconditions_1
3D CBCACONNHsample_2not availableconditions_1
3D HN(CA)COsample_2not availableconditions_1
3D CCC(CO)NNHsample_2not availableconditions_1
3D H(CCO)NNHsample_2not availableconditions_1
3D HBHA(CO)NNHsample_2not availableconditions_1
3D HCCH TOCSYsample_2not availableconditions_1
3D HCCH COSYsample_2not availableconditions_1
3D 15N-NOESY-HSQCsample_2not availableconditions_1
3D 13C-HMQC-NOESYsample_2not availableconditions_1
3D 13C-NOESY-HSQCsample_2not availableconditions_1
1H13C-HMQCsample_3not availableconditions_1
2D TOCSYnot availablenot availableconditions_1

Software:

XWIN-NMR v2.6, Bruker - processing

ANALYSIS v1.0.9-10 - assignment

CYANA v2.0 - automated NOESY peak assignment

X-PLOR NIH - structure refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAF84704 BAG37770 BAG51866 BAI46194
GB AAF80227 AAH20632 AAK58589 ABM82319 ABM85495
REF NP_570138 XP_001113061 XP_002809731 XP_003264531 XP_003820638
SP Q9NS28

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts