BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7116

Title: The solution structure of PHS018 from pyrococcus horikoshii   PubMed: 17027498

Deposition date: 2006-05-17 Original release date: 2006-11-13

Authors: Coles, M.; Hulko, M.; Truffault, V.; Martin, J.; Lupas, A.

Citation: Coles, M.; Hulko, M.; Djuranovic, S.; Truffault, V.; Koretke, K.; Martin, J.; Lupas, A.. "Common evolutionary origin of swapped-hairpin and double-psi beta barrels"  Structure 14, 1489-1498 (2006).

Assembly members:
PhS018, polymer, 92 residues, Formula weight is not available

Natural source:   Common Name: Pyrococcus horikoshii   Taxonomy ID: 53953   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus horikoshii

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PhS018: MDVLAKFHTTVHRIGRIIIP AGTRKFYGIEQGDFVEIKIV KYEGEEPKEGTFTARVGEQG SVIIPKALRDVIGIKPGEVI EVLLLGHYKPRN

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts89
1H chemical shifts700
coupling constants83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
192aa long hypothetical protein1

Entities:

Entity 1, 92aa long hypothetical protein 92 residues - Formula weight is not available

1   METASPVALLEUALALYSPHEHISTHRTHR
2   VALHISARGILEGLYARGILEILEILEPRO
3   ALAGLYTHRARGLYSPHETYRGLYILEGLU
4   GLNGLYASPPHEVALGLUILELYSILEVAL
5   LYSTYRGLUGLYGLUGLUPROLYSGLUGLY
6   THRPHETHRALAARGVALGLYGLUGLNGLY
7   SERVALILEILEPROLYSALALEUARGASP
8   VALILEGLYILELYSPROGLYGLUVALILE
9   GLUVALLEULEULEUGLYHISTYRLYSPRO
10   ARGASN

Samples:

sample_1: PhS018 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_2: PhS018, [U-15N], 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_3: PhS018, [U-13C; U-15N], 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 80 mM; pH: 3.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CCH-COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

X-PLOR vNIH-2.9.7 - refinement, structure solution

xwinnmr v3.5 - processing

NMRView v5.0.15 - data analysis

NMR spectrometers:

  • BRUKER DMX 900 MHz
  • BRUKER DMX 600 MHz

Related Database Links:

PDB
DBJ BAA29497
REF NP_142395 WP_010884522

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts