BMRB Entry 7131
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7131
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Title: Solution structure of IGF2R domain 11 PubMed: 17850746
Deposition date: 2006-05-24 Original release date: 2008-08-13
Authors: Williams, Christopher; Crump, Matthew
Citation: Williams, Christopher; Rezgui, Dellel; Prince, Stuart; Zaccheo, Oliver; Foulstone, Emily; Forbes, Briony; Norton, Raymond; Crosby, John; Hassan, A.; Crump, Matthew. "Structural insights into the interaction of insulin-like growth factor 2 with IGF2R domain 11." Structure 15, 1065-1078 (2007).
Assembly members:
domain 11, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
domain 11: MKSNEHDDCQVTNPSTGHLF
DLSSLSGRAGFTAAYSEKGL
VYMSICGENENCPPGVGACF
GQTRISVGKANKRLRYVDQV
LQLVYKDGSPCPSKSGLSYK
SVISFVCRPEAGPTNRPMLI
SLDKQTCTLFFSWHTPLACE
QATEKHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 595 |
15N chemical shifts | 147 |
1H chemical shifts | 899 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | domian 11 | 1 |
Entities:
Entity 1, domian 11 151 residues - Formula weight is not available
C-terminal His tag from vector
1 | MET | LYS | SER | ASN | GLU | HIS | ASP | ASP | CYS | GLN | ||||
2 | VAL | THR | ASN | PRO | SER | THR | GLY | HIS | LEU | PHE | ||||
3 | ASP | LEU | SER | SER | LEU | SER | GLY | ARG | ALA | GLY | ||||
4 | PHE | THR | ALA | ALA | TYR | SER | GLU | LYS | GLY | LEU | ||||
5 | VAL | TYR | MET | SER | ILE | CYS | GLY | GLU | ASN | GLU | ||||
6 | ASN | CYS | PRO | PRO | GLY | VAL | GLY | ALA | CYS | PHE | ||||
7 | GLY | GLN | THR | ARG | ILE | SER | VAL | GLY | LYS | ALA | ||||
8 | ASN | LYS | ARG | LEU | ARG | TYR | VAL | ASP | GLN | VAL | ||||
9 | LEU | GLN | LEU | VAL | TYR | LYS | ASP | GLY | SER | PRO | ||||
10 | CYS | PRO | SER | LYS | SER | GLY | LEU | SER | TYR | LYS | ||||
11 | SER | VAL | ILE | SER | PHE | VAL | CYS | ARG | PRO | GLU | ||||
12 | ALA | GLY | PRO | THR | ASN | ARG | PRO | MET | LEU | ILE | ||||
13 | SER | LEU | ASP | LYS | GLN | THR | CYS | THR | LEU | PHE | ||||
14 | PHE | SER | TRP | HIS | THR | PRO | LEU | ALA | CYS | GLU | ||||
15 | GLN | ALA | THR | GLU | LYS | HIS | HIS | HIS | HIS | HIS | ||||
16 | HIS |
Samples:
sample_1: domain 11, [U-95% 13C; U-95% 15N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%
sample_2: domain 11, [U-95% 15N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%
sample_3: domain 11, [U-99% 12C; U-99% 14N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%
conditions_1: pH: 5.5; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H15N_HSQC | sample_1 | not available | conditions_1 |
CBCACONH | not available | not available | conditions_1 |
HNCACB | not available | not available | conditions_1 |
HNCO | not available | not available | conditions_1 |
HNCACO | not available | not available | conditions_1 |
HNCA | not available | not available | conditions_1 |
HNCOCA | not available | not available | conditions_1 |
HNHA | not available | not available | conditions_1 |
HCCH-TCOSY | not available | not available | conditions_1 |
15N HSQC-NOESY | not available | not available | conditions_1 |
15N HSQC-TOCSY | not available | not available | conditions_1 |
1H-1H NOESY | not available | not available | conditions_1 |
1H-1H TOCSY | not available | not available | conditions_1 |
13C/15N NOESY | not available | not available | conditions_1 |
Software:
CNS v1.2 - structure calculation
NMRPipe_NMRDraw - data processing
SPARKY v3.12 - assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts