BMRB Entry 7248
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7248
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Title: 1H, 13C, 15N Chemical Shift Assignments for Mesd12-155 PubMed: 19636811
Deposition date: 2006-08-02 Original release date: 2006-10-20
Authors: Chen, Jianglei; Wang, Jianjun
Citation: Chen, Jianglei; Bu, Guojun; Wang, Jianjun. "A complete NMR spectral assignment of the conserved region of the MESD protein, MESD(12-155)" Biomol. NMR Assignments 1, 3-5 (2007).
Assembly members:
mesd12-155, polymer, 145 residues, 16670 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
mesd12-155: RKKKDIRDYNDADMARLLEQ
WEKDDDIEEGDLPEHKRPSA
PIDFSKLDPGKPESILKMTK
KGKTLMMFVTVSGNPTEKET
EEITSLWQGSLFNANYDVQR
FIVGSDRAIFMLRDGSYAWE
IKDFLVSQDRCAEVTLEGQM
YPGKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 564 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 932 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mesd(12-155) | 1 |
Entities:
Entity 1, Mesd(12-155) 145 residues - 16670 Da.
| 1 | ARG | LYS | LYS | LYS | ASP | ILE | ARG | ASP | TYR | ASN | ||||
| 2 | ASP | ALA | ASP | MET | ALA | ARG | LEU | LEU | GLU | GLN | ||||
| 3 | TRP | GLU | LYS | ASP | ASP | ASP | ILE | GLU | GLU | GLY | ||||
| 4 | ASP | LEU | PRO | GLU | HIS | LYS | ARG | PRO | SER | ALA | ||||
| 5 | PRO | ILE | ASP | PHE | SER | LYS | LEU | ASP | PRO | GLY | ||||
| 6 | LYS | PRO | GLU | SER | ILE | LEU | LYS | MET | THR | LYS | ||||
| 7 | LYS | GLY | LYS | THR | LEU | MET | MET | PHE | VAL | THR | ||||
| 8 | VAL | SER | GLY | ASN | PRO | THR | GLU | LYS | GLU | THR | ||||
| 9 | GLU | GLU | ILE | THR | SER | LEU | TRP | GLN | GLY | SER | ||||
| 10 | LEU | PHE | ASN | ALA | ASN | TYR | ASP | VAL | GLN | ARG | ||||
| 11 | PHE | ILE | VAL | GLY | SER | ASP | ARG | ALA | ILE | PHE | ||||
| 12 | MET | LEU | ARG | ASP | GLY | SER | TYR | ALA | TRP | GLU | ||||
| 13 | ILE | LYS | ASP | PHE | LEU | VAL | SER | GLN | ASP | ARG | ||||
| 14 | CYS | ALA | GLU | VAL | THR | LEU | GLU | GLY | GLN | MET | ||||
| 15 | TYR | PRO | GLY | LYS | GLY |
Samples:
sample_1: mesd12-155, [U-13C; U-15N], 1.5 ± 0.1 mM
conditions_1: pH: 7; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| H(CC-tocsy)-NNH | sample_1 | not available | conditions_1 |
| C(CC-tocsy)-NNH | sample_1 | not available | conditions_1 |
| 15N-edited NOESY-HSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 11076 16213 |
| PDB | |
| DBJ | BAB25865 BAC27617 BAC36471 BAC36476 |
| GB | AAG33621 AAH14742 AAH85892 EDL06865 EDL06866 |
| REF | NP_001008346 NP_075892 XP_003511746 XP_005088080 XP_006885181 |
| SP | Q5U2R7 Q9ERE7 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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