BMRB Entry 7281
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7281
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Title: NMR STRUCTURE OF PROTEIN YJBR FROM ESCHERICHIA COLI;NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER226 PubMed: 17266124
Deposition date: 2006-08-29 Original release date: 2006-10-23
Authors: SINGARAPU, KIRAN; GAOHUA, LIU; XIAO, RONG; SHETTY, KARISHMA; GAETANO, MONTELIONE; SZYPERSKI, THOMAS
Citation: Singarapu, Kiran; Liu, Gaohua; Xiao, Rong; Bertonati, Claudia; Honig, Barry; Montelione, Gaetano; Szyperski, Thomas. "NMR structure of protein yjbR from Escherichia coli reveals 'double-wing' DNA binding motif." Proteins 67, 501-504 (2007).
Assembly members:
protein yjbR, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eukaryota Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
protein yjbR: MTISELLQYCMAKPGAEQSV
HNDWKATQIKVEDVLFAMVK
EVENRPAVSLKTSPELAELL
RQQHSDVRPSRHLNKAHWST
VYLDGSLPDSQIYYLVDASY
QQAVNLLPEEKRKLLVQLLE
HHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 507 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 845 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein yjbR monomer | 1 |
Entities:
Entity 1, protein yjbR monomer 126 residues - Formula weight is not available
| 1 | MET | THR | ILE | SER | GLU | LEU | LEU | GLN | TYR | CYS | ||||
| 2 | MET | ALA | LYS | PRO | GLY | ALA | GLU | GLN | SER | VAL | ||||
| 3 | HIS | ASN | ASP | TRP | LYS | ALA | THR | GLN | ILE | LYS | ||||
| 4 | VAL | GLU | ASP | VAL | LEU | PHE | ALA | MET | VAL | LYS | ||||
| 5 | GLU | VAL | GLU | ASN | ARG | PRO | ALA | VAL | SER | LEU | ||||
| 6 | LYS | THR | SER | PRO | GLU | LEU | ALA | GLU | LEU | LEU | ||||
| 7 | ARG | GLN | GLN | HIS | SER | ASP | VAL | ARG | PRO | SER | ||||
| 8 | ARG | HIS | LEU | ASN | LYS | ALA | HIS | TRP | SER | THR | ||||
| 9 | VAL | TYR | LEU | ASP | GLY | SER | LEU | PRO | ASP | SER | ||||
| 10 | GLN | ILE | TYR | TYR | LEU | VAL | ASP | ALA | SER | TYR | ||||
| 11 | GLN | GLN | ALA | VAL | ASN | LEU | LEU | PRO | GLU | GLU | ||||
| 12 | LYS | ARG | LYS | LEU | LEU | VAL | GLN | LEU | LEU | GLU | ||||
| 13 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: protein yjbR, [U-13C; U-15N], 1.16 mM; DTT 100 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; NaN3 0.02%; D2O 5%; H2O 95%
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 4,3D, GFT HNNCABCA | sample_1 | not available | conditions_1 |
| 4,3D, GFT CABCACONNH | sample_1 | not available | conditions_1 |
| 4,3D, GFT HABCABCONNH | sample_1 | not available | conditions_1 |
| 15N-13C, 3D NOESY | sample_1 | not available | conditions_1 |
| 2D 1H13C HSQC | sample_1 | not available | conditions_1 |
| 4,3D, GFT HCCH COSY | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D 1H, 13C aro NOESY | sample_1 | not available | conditions_1 |
| 3D, HCCH COSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- VARIAN UNITY 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38462 BAE78059 BAG79875 BAI28319 BAI33496 |
| EMBL | CAP78523 CAQ34406 CAQ91581 CAR01037 CAR05706 |
| GB | AAC43151 AAC77027 AAG59255 AAN45569 AAN83473 |
| REF | NP_290690 NP_313066 NP_418481 NP_709862 NP_756899 |
| SP | P0AF50 P0AF51 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts