BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7339

Title: Solution structure of Arabidopsis thaliana protein At1g70830, a member of the major latex protein family   PubMed: 19326460

Deposition date: 2006-11-09 Original release date: 2010-11-16

Authors: Volkman, B.; de la Cruz, N.; Lytle, B.; Peterson, F.

Citation: Lytle, Betsy; Song, Jikui; de la Cruz, Norberto; Peterson, Francis; Johnson, Kenneth; Bingman, Craig; Phillips, George; Volkman, Brian. "Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds."  Proteins 76, 237-243 (2009).

Assembly members:
MLP-like_protein_28, polymer, 166 residues, Formula weight is not available

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MLP-like_protein_28: GHHHHHHLETEASSLVGKLE TDVEIKASADKFHHMFAGKP HHVSKASPGNIQGCDLHEGD WGTVGSIVFWNYVHDGEAKV AKERIEAVEPDKNLITFRVI EGDLMKEYKSFLLTIQVTPK PGGPGSIVHWHLEYEKISEE VAHPETLLQFCVEVSKEIDE HLLAEE

Data sets:
Data typeCount
13C chemical shifts665
15N chemical shifts156
1H chemical shifts1047

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MLP-like protein 281

Entities:

Entity 1, MLP-like protein 28 166 residues - Formula weight is not available

1   GLYHISHISHISHISHISHISLEUGLUTHR
2   GLUALASERSERLEUVALGLYLYSLEUGLU
3   THRASPVALGLUILELYSALASERALAASP
4   LYSPHEHISHISMETPHEALAGLYLYSPRO
5   HISHISVALSERLYSALASERPROGLYASN
6   ILEGLNGLYCYSASPLEUHISGLUGLYASP
7   TRPGLYTHRVALGLYSERILEVALPHETRP
8   ASNTYRVALHISASPGLYGLUALALYSVAL
9   ALALYSGLUARGILEGLUALAVALGLUPRO
10   ASPLYSASNLEUILETHRPHEARGVALILE
11   GLUGLYASPLEUMETLYSGLUTYRLYSSER
12   PHELEULEUTHRILEGLNVALTHRPROLYS
13   PROGLYGLYPROGLYSERILEVALHISTRP
14   HISLEUGLUTYRGLULYSILESERGLUGLU
15   VALALAHISPROGLUTHRLEULEUGLNPHE
16   CYSVALGLUVALSERLYSGLUILEASPGLU
17   HISLEULEUALAGLUGLU

Samples:

sample: MLP-like protein 28, [U-13C; U-15N], 0.7 mM; bis-tris, [U-2H], 10 mM; Dithiothreitol, none, 5 mM; H2O, none, 95%; D2O, none, 5%

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsampleisotropicsample_conditions_1
3D 13C-separated NOESYsampleisotropicsample_conditions_1
3D 13C-separated NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v2004, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C.; Guntert, P.; Billeter, M.; Wuthrich, K. - data analysis

SPSCAN v1.1.0, R.W. Glaser - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. -

X-PLOR NIH v2.9.3, SCHWIETERS, C.D., KUSZEWSKI, J.J., TJANDRA, N., CLORE, G.M. - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15720
PDB
GB AEE35123 AEE35125
REF NP_001077806 NP_001117579

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts