BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11580

Title: NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion

Deposition date: 2014-11-05 Original release date: 2016-07-13

Authors: Mahajan, Mukesh; Bhattacharjya, Surajit

Citation: Mahajan, Mukesh; Bhattacharjya, Surajit. "NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion"  Biochim. Biophys. Acta ., .-..

Assembly members:
Fusion_Peptide, polymer, 19 residues, 2280.706 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Fusion_Peptide: MWKTPTLKYFGGFNFSQIL

Data sets:
Data typeCount
13C chemical shifts17
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Fusion peptide1

Entities:

Entity 1, Fusion peptide 19 residues - 2280.706 Da.

1   METTRPLYSTHRPROTHRLEULYSTYRPHE
2   GLYGLYPHEASNPHESERGLNILELEU

Samples:

sample_1: DPC, [U-99% 2H], 125 mM; Fusion Peptide 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 4.7; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Structure calculation

SPARKY v3.113, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz