BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11582

Title: NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion

Deposition date: 2014-11-06 Original release date: 2016-07-13

Authors: Mahajan, Mukesh; Bhattacharjya, Surajit

Citation: Mahajan, Mukesh; Bhattacharjya, Surajit. "NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion"  Biochim. Biophys. Acta ., .-..

Assembly members:
Internal_fusion_peptide, polymer, 16 residues, 1816.184 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Internal_fusion_peptide: GAALQIPFAMQMAYRF

Data sets:
Data typeCount
13C chemical shifts15
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Internal fusion peptide1

Entities:

Entity 1, Internal fusion peptide 16 residues - 1816.184 Da.

1   GLYALAALALEUGLNILEPROPHEALAMET
2   GLNMETALATYRARGPHE

Samples:

sample_1: Internal fusion peptide 0.5 mM; DPC, [U-99% 2H], 125 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 4.7; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Structure Calculation

NMR spectrometers:

  • Bruker Avance 600 MHz