BMRB Entry 15481
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15481
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Title: 15N and 1HN assignments of the EVH1 domain of human HOMER3A
Deposition date: 2007-09-20 Original release date: 2007-09-27
Authors: Higman, Victoria; Schmieder, Peter; Diehl, Anne; Bishop, Shurene; Oschkinat, Hartmut; Arrowsmith, Cheryl; Wiegelt, Johan; Edwards, Aled; Sundstrom, Michael; Ball, Linda
Citation: Higman, Victoria; Schmieder, Peter; Bishop, Shurene; Diehl, Anne; Oschkinat, Hartmut; Arrowsmith, Cheryl; Edwards, Aled; Sundstrom, Michael; Wiegelt, Johan; Ball, Linda. "15N and 1HN assignments of the EVH1 domain of human HOMER3A" Biomol. NMR Assignments ., .-..
Assembly members:
HOMER3A_EVH1_domain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HOMER3A_EVH1_domain: SMAREQPIFSTRAHVFQIDP
ATKRNWIPAGKHALTVSYFY
DATRNVYRIISIGGAKAIIN
STVTPNMTFTKTSQKFGQWA
DSRANTVYGLGFASEQHLTQ
FAEKFQEVKEAARLARE
- assigned_chemical_shifts
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 114 |
| T1 relaxation values | 91 |
| T2 relaxation values | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HOMER3A EVH1 domain | 1 |
Entities:
Entity 1, HOMER3A EVH1 domain 117 residues - Formula weight is not available
The first two residues (SM) are from the vector.
| 1 | SER | MET | ALA | ARG | GLU | GLN | PRO | ILE | PHE | SER | ||||
| 2 | THR | ARG | ALA | HIS | VAL | PHE | GLN | ILE | ASP | PRO | ||||
| 3 | ALA | THR | LYS | ARG | ASN | TRP | ILE | PRO | ALA | GLY | ||||
| 4 | LYS | HIS | ALA | LEU | THR | VAL | SER | TYR | PHE | TYR | ||||
| 5 | ASP | ALA | THR | ARG | ASN | VAL | TYR | ARG | ILE | ILE | ||||
| 6 | SER | ILE | GLY | GLY | ALA | LYS | ALA | ILE | ILE | ASN | ||||
| 7 | SER | THR | VAL | THR | PRO | ASN | MET | THR | PHE | THR | ||||
| 8 | LYS | THR | SER | GLN | LYS | PHE | GLY | GLN | TRP | ALA | ||||
| 9 | ASP | SER | ARG | ALA | ASN | THR | VAL | TYR | GLY | LEU | ||||
| 10 | GLY | PHE | ALA | SER | GLU | GLN | HIS | LEU | THR | GLN | ||||
| 11 | PHE | ALA | GLU | LYS | PHE | GLN | GLU | VAL | LYS | GLU | ||||
| 12 | ALA | ALA | ARG | LEU | ALA | ARG | GLU |
Samples:
sample_1: HOMER3A EVH1 domain, [U-99% 15N], 0.7 mM; potassium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.15 mM; DTT 1 mM
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 297 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC - T1 series | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC - T2 series | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker Biospin - collection
TOPSPIN v1.3, Bruker Biospin - processing
ANALYSIS v1.0.15, CCPNMR - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| SWS | Q9NSC5 |
| PDB | |
| NCBI | gi:21361157 |
| DBJ | BAA35110 BAG36707 BAG54040 |
| EMBL | CAB75536 CAB75543 CAB75544 CAB75545 CAC35223 |
| GB | AAB81545 AAC71025 AAC71029 AAH05773 AAH12113 |
| REF | NP_001139193 NP_001139194 NP_001139196 NP_001139625 NP_001244618 |
| SP | Q99JP6 Q9NSC5 Q9Z2X5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts