BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16608

Title: NMR Structure of Proinsulin   PubMed: 20106974

Authors: Yang, Yanwu; Hua, Qing-hua; Mackin, Robert; Weiss, Michael

Citation: Yang, Yanwu; Hua, Qing-Xin; Liu, Jin; Shimizu, Eri; Choquette, Meredith; Mackin, Robert; Weiss, Michael. "Solution Structure of Proinsulin: CONNECTING DOMAIN FLEXIBILITY AND PROHORMONE PROCESSING."  J. Biol. Chem. 285, 7847-7851 (2010).

Assembly members:
Proinsulin, polymer, 86 residues, 9379.647 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Proinsulin: FVNQHLCGSDLVEALYLVCG ERGFFYTKPTRREAEDLQVG QVELGGGPGAGSLQPLALEG SLQKRGIVEQCCTSICSLYQ LENYCN

Data sets:
Data typeCount
13C chemical shifts271
15N chemical shifts83
1H chemical shifts568

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Proinsulin1

Entities:

Entity 1, Proinsulin 86 residues - 9379.647 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERASP
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRLYSPROTHR
4   ARGARGGLUALAGLUASPLEUGLNVALGLY
5   GLNVALGLULEUGLYGLYGLYPROGLYALA
6   GLYSERLEUGLNPROLEUALALEUGLUGLY
7   SERLEUGLNLYSARGGLYILEVALGLUGLN
8   CYSCYSTHRSERILECYSSERLEUTYRGLN
9   LEUGLUASNTYRCYSASN

Samples:

sample_1: proinsulin, [U-100% 13C; U-100% 15N], 0.3 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker MSL 700 MHz

Related Database Links:

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