BMRB Entry 18903
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18903
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Title: Relaxation study of Delta subunit of RNA polymerase from Bacillus subtilis PubMed: 24515886
Deposition date: 2012-12-16 Original release date: 2014-02-14
Authors: Kaderavek, Pavel; Sklenar, Vladimir; Zidek, Lukas; Rabatinova, Alzbeta; Krasny, Libor
Citation: Kadeavek, Pavel; Zapletal, Vojtch; Rabatinova, Albeta; Krasny, Libor; Sklena, Vladimir; Zidek, Luka. "Spectral density mapping protocols for analysis of molecular motions in disordered proteins." J. Biomol. NMR ., .-. (2014).
Assembly members:
delta, polymer, 172 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
delta: GIKQYSQEELKEMALVEIAH
ELFEEHKKPVPFQELLNEIA
SLLGVKKEELGDRIAQFYTD
LNIDGRFLALSDQTWGLRSW
YPYDQLDEETQPTVKAKKKK
AKKAVEEDLDLDEFEEIDED
DLDLDEVEEELDLEADDFDE
EDLDEDDDDLEIEEDIIDED
DEDYDDEEEEIK
- assigned_chemical_shifts
- dipole_CSA_cross_correlation_relaxation
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| dipole-CSA cross correlation relaxation values | 264 |
| heteronuclear NOE values | 96 |
| T1 relaxation values | 98 |
| T2 relaxation values | 203 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | delta | 1 |
Entities:
Entity 1, delta 172 residues - Formula weight is not available
| 1 | GLY | ILE | LYS | GLN | TYR | SER | GLN | GLU | GLU | LEU | ||||
| 2 | LYS | GLU | MET | ALA | LEU | VAL | GLU | ILE | ALA | HIS | ||||
| 3 | GLU | LEU | PHE | GLU | GLU | HIS | LYS | LYS | PRO | VAL | ||||
| 4 | PRO | PHE | GLN | GLU | LEU | LEU | ASN | GLU | ILE | ALA | ||||
| 5 | SER | LEU | LEU | GLY | VAL | LYS | LYS | GLU | GLU | LEU | ||||
| 6 | GLY | ASP | ARG | ILE | ALA | GLN | PHE | TYR | THR | ASP | ||||
| 7 | LEU | ASN | ILE | ASP | GLY | ARG | PHE | LEU | ALA | LEU | ||||
| 8 | SER | ASP | GLN | THR | TRP | GLY | LEU | ARG | SER | TRP | ||||
| 9 | TYR | PRO | TYR | ASP | GLN | LEU | ASP | GLU | GLU | THR | ||||
| 10 | GLN | PRO | THR | VAL | LYS | ALA | LYS | LYS | LYS | LYS | ||||
| 11 | ALA | LYS | LYS | ALA | VAL | GLU | GLU | ASP | LEU | ASP | ||||
| 12 | LEU | ASP | GLU | PHE | GLU | GLU | ILE | ASP | GLU | ASP | ||||
| 13 | ASP | LEU | ASP | LEU | ASP | GLU | VAL | GLU | GLU | GLU | ||||
| 14 | LEU | ASP | LEU | GLU | ALA | ASP | ASP | PHE | ASP | GLU | ||||
| 15 | GLU | ASP | LEU | ASP | GLU | ASP | ASP | ASP | ASP | LEU | ||||
| 16 | GLU | ILE | GLU | GLU | ASP | ILE | ILE | ASP | GLU | ASP | ||||
| 17 | ASP | GLU | ASP | TYR | ASP | ASP | GLU | GLU | GLU | GLU | ||||
| 18 | ILE | LYS |
Samples:
sample_1: delta, [U-100% 15N], 8.8 mg/mL; D2O, [U-2H], 10%; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; H2O 90%
sample_conditions_1: ionic strength: 30 mM; pH: 6.6; pressure: 1 atm; temperature: 300.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D R1 15N,1H correlation | sample_1 | isotropic | sample_conditions_1 |
| 2D R2 15N,1H correlation | sample_1 | isotropic | sample_conditions_1 |
| 2D NOE 15N,1H correlation | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N (coupled),1H correlation | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N,1H correlation - transverse cross-correlated cross-relaxation (symmetrical reconversion) | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N,1H correlation - longitudinal cross-correlated cross-relaxation (symmetrical reconversion) | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
Relax, d, Gooley,, 18903 - data analysis
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz