BMRB Entry 20086
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20086
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Title: Helical Agonist of Nociceptin (NOP) Receptor PubMed: 21067234
Deposition date: 2009-07-23 Original release date: 2011-06-03
Authors: Hill, Timothy; Fairlie, David; Hoang, Huy
Citation: Harrison, Rosemary; Ruiz-Gomez, Gloria; Hill, Timothy; Chow, Shiao; Shepherd, Nicholas; Lohman, Rink-Jan; Abbenante, Giovanni; Hoang, Huy; Fairlie, David. "Novel Helix-Constrained Nociceptin Derivatives Are Potent Agonists and Antagonists of ERK Phosphorylation and Thermal Analgesia in Mice." J. Med. Chem. 53, 8400-8408 (2010).
Assembly members:
Nociceptin_Antagonist, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Nociceptin_Antagonist: FGGFTGKRKSDRKKANQD
- assigned_chemical_shifts
- coupling_constants
Data type | Count |
1H chemical shifts | 82 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Nociceptin Antagonist | 1 |
Entities:
Entity 1, Nociceptin Antagonist 18 residues - Formula weight is not available
1 | PHE | GLY | GLY | PHE | THR | GLY | LYS | ARG | LYS | SER | ||||
2 | ASP | ARG | LYS | LYS | ALA | ASN | GLN | ASP |
Samples:
sample_1: Nociceptin Antagonist 3 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.5; temperature: 288 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz