BMRB Entry 27688
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27688
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Title: Chemical Shift Assignments for Interleukin-36beta isoform-2 PubMed: 30758717
Deposition date: 2018-11-09 Original release date: 2019-04-25
Authors: Ohlenschlaeger, Oliver; Kumar, Amit
Citation: Kumar, Amit; Wissbrock, Amelie; Bellstedt, Peter; Lang, Andras; Ramachandran, Ramadurai; Wiedemann, Christoph; Imhof, Diana; Ohlenschlaeger, Oliver. "(1)H, (13)C, and (15)N resonance assignments of the cytokine interleukin-36beta isoform-2" Biomol. NMR Assignments 13, 155-161 (2019).
Assembly members:
IL36B2, polymer, 153 residues, 17231.86 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
IL36B2: REAAPKSYAIRDSRQMVWVL
SGNSLIAAPLSRSIKPVTLH
LIACRDTEFSDKEKGNMVYL
GIKGKDLCLFCAEIQGKPTL
QLKEKNIMDLYVEKKAQKPF
LFFHNKEGSTSVFQSVSYPG
WFIATSTTSGQPIFLTKERG
ITNNTNFYLDSVE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 646 |
15N chemical shifts | 156 |
1H chemical shifts | 985 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | IL36B2 | 1 |
Entities:
Entity 1, IL36B2 153 residues - 17231.86 Da.
1 | ARG | GLU | ALA | ALA | PRO | LYS | SER | TYR | ALA | ILE | ||||
2 | ARG | ASP | SER | ARG | GLN | MET | VAL | TRP | VAL | LEU | ||||
3 | SER | GLY | ASN | SER | LEU | ILE | ALA | ALA | PRO | LEU | ||||
4 | SER | ARG | SER | ILE | LYS | PRO | VAL | THR | LEU | HIS | ||||
5 | LEU | ILE | ALA | CYS | ARG | ASP | THR | GLU | PHE | SER | ||||
6 | ASP | LYS | GLU | LYS | GLY | ASN | MET | VAL | TYR | LEU | ||||
7 | GLY | ILE | LYS | GLY | LYS | ASP | LEU | CYS | LEU | PHE | ||||
8 | CYS | ALA | GLU | ILE | GLN | GLY | LYS | PRO | THR | LEU | ||||
9 | GLN | LEU | LYS | GLU | LYS | ASN | ILE | MET | ASP | LEU | ||||
10 | TYR | VAL | GLU | LYS | LYS | ALA | GLN | LYS | PRO | PHE | ||||
11 | LEU | PHE | PHE | HIS | ASN | LYS | GLU | GLY | SER | THR | ||||
12 | SER | VAL | PHE | GLN | SER | VAL | SER | TYR | PRO | GLY | ||||
13 | TRP | PHE | ILE | ALA | THR | SER | THR | THR | SER | GLY | ||||
14 | GLN | PRO | ILE | PHE | LEU | THR | LYS | GLU | ARG | GLY | ||||
15 | ILE | THR | ASN | ASN | THR | ASN | PHE | TYR | LEU | ASP | ||||
16 | SER | VAL | GLU |
Samples:
sample_1: IL36B2, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM
sample_2: IL36B2, [U-100% 15N], 1 mM; sodium phosphate 20 mM
sample_3: IL36B2, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection, processing
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts