BMRB Entry 6434

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PDB ID: 1z1z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6434
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and Sidechain Assignments of the Lambda Bacteriophage Tail Assembly Protein gpU

Deposition date: 2004-12-16 Original release date: 2005-07-26

Authors: Edmonds, Lizbeth; Thirumoorthy, Ramanan; Liu, Amanda; Davidson, Alan; Donaldson, Logan

Citation: Edmonds, Lizbeth; Thirumoorthy, Ramanan; Liu, Amanda; Davidson, Alan; Donaldson, Logan. "Letter to the Editor: NMR assignment of the gpU tail protein from lambda bacteriophage" J. Biomol. NMR 32, 91-92 (2005).

Assembly members:
gpU, polymer, 150 residues, 16681 Da.
MG, non-polymer, 24.305 Da.

Natural source: Common Name: bacteriophage lambda Taxonomy ID: 10710 Superkingdom: Viruses Kingdom: not available Genus/species: Lambda-like viruses lambda

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
gpU: GSSHHHHHHSSGLVPRGSHM KHTELRAAVLDALEKHDTGA TFFDGRPAVFDEADFPAVAV YLTGAEYTGEELDSDTWQAE LHIEVFLPAQVPDSELDAWM ESRIYPVMSDIPALSDLITS MVASGYDYRRDDDAGLWSSA DLTYVITYEM

Data sets:

assigned_chemical_shifts
- set_1

Data type	Count
13C chemical shifts	546
1H chemical shifts	854
15N chemical shifts	137

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GPU monomer	1
2	MG_2+	2

Entities:

Entity 1, GPU monomer 150 residues - 16681 Da.

1	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS	SER
2	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS	MET
3	LYS	HIS	THR	GLU	LEU	ARG	ALA	ALA	VAL	LEU
4	ASP	ALA	LEU	GLU	LYS	HIS	ASP	THR	GLY	ALA
5	THR	PHE	PHE	ASP	GLY	ARG	PRO	ALA	VAL	PHE
6	ASP	GLU	ALA	ASP	PHE	PRO	ALA	VAL	ALA	VAL
7	TYR	LEU	THR	GLY	ALA	GLU	TYR	THR	GLY	GLU
8	GLU	LEU	ASP	SER	ASP	THR	TRP	GLN	ALA	GLU
9	LEU	HIS	ILE	GLU	VAL	PHE	LEU	PRO	ALA	GLN
10	VAL	PRO	ASP	SER	GLU	LEU	ASP	ALA	TRP	MET
11	GLU	SER	ARG	ILE	TYR	PRO	VAL	MET	SER	ASP
12	ILE	PRO	ALA	LEU	SER	ASP	LEU	ILE	THR	SER
13	MET	VAL	ALA	SER	GLY	TYR	ASP	TYR	ARG	ARG
14	ASP	ASP	ASP	ALA	GLY	LEU	TRP	SER	SER	ALA
15	ASP	LEU	THR	TYR	VAL	ILE	THR	TYR	GLU	MET

Entity 2, MG_2+ - Mg - 24.305 Da.

1

MG

Samples:

sample_1: gpU, [U-98% 13C; U-98% 15N], 0.8 mM; deutero-Tris, [U-d10], 5 mM; KCl 50 mM; sodium azide 0.02%

condition-1: pH: 7.8; temperature: 293 K; ionic strength: 0.05 M

Experiments:

Name	Sample	Sample state	Sample conditions
Bruker 600 MHz at York University	sample_1	not available	condition-1
Varian 800 MHz at NANUC, Edmonton, AB, Canada	sample_1	not available	condition-1
Varian 800 MHz at QANUC, Montreal, QC, Canada	sample_1	not available	condition-1

Software:

NMRView v5.2 - assignment

nmrPipe - processing

NMR spectrometers:

Bruker Avance DRX 600 MHz
Varian Inova 800 MHz

PDB	1Z1Z 3FZB
DBJ	BAB35062 BAG76127 BAG77177 BAG77581 BAI23947
EMBL	CAQ31258 CAQ97656 CAQ98436 CAR12634 CAR16658
GB	AAA96544 AAG55989 AAN81616 ABE06803 ABG69187
REF	NP_040591 NP_287377 NP_309666 NP_755046 WP_000352477
SP	P03732

Biological Magnetic Resonance Data Bank