BMRB Entry 7083
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7083
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Title: Chemical shifts of SBD from Rhizopu oryzae glucoamylase PubMed: 17117925
Deposition date: 2006-04-24 Original release date: 2007-05-02
Authors: Liu, Yu-Nan; Lyu, Ping-Chaing
Citation: Liu, Yu-Nan; Lai, Yen-Ting; Chou, Wei-I; Chang, Margaret; Lyu, Ping-Chiang. "Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase." Biochem. J. 403, 21-30 (2007).
Assembly members:
SBD, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Rhizopus oryzae Taxonomy ID: 64495 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Rhizopus oryzae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SBD: ASIPSSASVQLDSYNYDGST
FSGKIYVKNIAYSKKVTVVY
ADGSDNWNNNGNIIAASFSG
PISGSNYEYWTFSASVKGIK
EFYIKYEVSGKTYYDNNNSA
NYQVST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 309 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 639 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SBD | 1 |
Entities:
Entity 1, SBD 106 residues - Formula weight is not available
| 1 | ALA | SER | ILE | PRO | SER | SER | ALA | SER | VAL | GLN | ||||
| 2 | LEU | ASP | SER | TYR | ASN | TYR | ASP | GLY | SER | THR | ||||
| 3 | PHE | SER | GLY | LYS | ILE | TYR | VAL | LYS | ASN | ILE | ||||
| 4 | ALA | TYR | SER | LYS | LYS | VAL | THR | VAL | VAL | TYR | ||||
| 5 | ALA | ASP | GLY | SER | ASP | ASN | TRP | ASN | ASN | ASN | ||||
| 6 | GLY | ASN | ILE | ILE | ALA | ALA | SER | PHE | SER | GLY | ||||
| 7 | PRO | ILE | SER | GLY | SER | ASN | TYR | GLU | TYR | TRP | ||||
| 8 | THR | PHE | SER | ALA | SER | VAL | LYS | GLY | ILE | LYS | ||||
| 9 | GLU | PHE | TYR | ILE | LYS | TYR | GLU | VAL | SER | GLY | ||||
| 10 | LYS | THR | TYR | TYR | ASP | ASN | ASN | ASN | SER | ALA | ||||
| 11 | ASN | TYR | GLN | VAL | SER | THR |
Samples:
sample_1: SBD 1 mM
sample_2: SBD, [U-98% 15N], 1 mM
sample_3: SBD, [U-98% 15N; U-98% 13C], 1 mM
conditions_1: ionic strength: 10 mM; pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | not available | not available | conditions_1 |
| HNCACB | not available | not available | conditions_1 |
| CBCA(CO)NH | not available | not available | conditions_1 |
| 1H_2DNOESY | not available | not available | conditions_1 |
| 1H_2D_TOCSY | not available | not available | conditions_1 |
Software:
SPARKY v3.111 -
NMR spectrometers:
- bruker avance 600 MHz
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts