BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15501

Title: Backbone assignment of human Proliferating Cell Nuclear Antigen.

Deposition date: 2007-10-02 Original release date: 2008-01-28

Authors: Sanchez, Ricardo; Torres, Daniel; Prieto, Jesus; Blanco, Francisco J.; Campos-Olivas, Ramon

Citation: Sanchez, Ricardo; Torres, Daniel; Prieto, Jesus; Blanco, Francisco J.; Campos-Olivas, Ramon. "Backbone Assignment of human Proliferating Cell Nuclear Antigen"  Biomol. NMR Assignments 1, 245-247 (2007).

Assembly members:
human_PCNA, polymer, 264 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human_PCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS

Data sets:
Data typeCount
13C chemical shifts778
15N chemical shifts261
1H chemical shifts279

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human PCNA, monomer 11
2human PCNA, monomer 21
3human PCNA, monomer 31

Entities:

Entity 1, human PCNA, monomer 1 264 residues - Formula weight is not available

Residues -3 to -1 are a non-native tag. The native sequence starts at residue number 4: MF...

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Samples:

sample_1: human PCNA, [U-99% 13C; U-100% 15N; U-93% 2H], 1.0 mM; sodium chloride 137.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 2.0 mM

sample_2: human PCNA, [U-15N]-Leu, 1.0 mM; sodium chloride 137.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 2.0 mM

sample_3: human PCNA, [U-15N]-Val, 1.0 mM; sodium chloride 137.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 2.0 mM

sample_4: human PCNA, [U-15N]-Ile, 1.0 mM; sodium chloride 137.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 2.0 mM

sample_5: human PCNA, [U-15N]-Phe, [U-15N]-Tyr, 1.0 mM; sodium chloride 137.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 2.0 mM

sample_conditions_1: ionic strength: 0.327 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
3D trHNCAsample_1isotropicsample_conditions_1
3D trHNCOsample_1isotropicsample_conditions_1
3D trHNCACBsample_1isotropicsample_conditions_1
4D 1H-15N,1H-15N NOESYsample_1isotropicsample_conditions_1
3D trHN(CA)COsample_1isotropicsample_conditions_1
3D trHN(CO)CACBsample_1isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17375 17376
PDB
DBJ BAB28355 BAB28557 BAC40240 BAE30230 BAE30376
EMBL CAA40938 CAA68261 CAG38740 CAG46598
GB AAA35736 AAA60040 AAG10077 AAG37435 AAH00491
REF NP_001029666 NP_001233697 NP_001253019 NP_001274649 NP_001278854
SP P04961 P12004 P17918 P57761 P61258
TPG DAA23435

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts