BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4131

Title: 1H, 15N, 13C chemical shifts of recombinant rat ferrocytochrome b5, B conformation

Deposition date: 1998-04-07 Original release date: 1998-08-12

Authors: Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald

Citation: Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald. "The Origin of Difference in the Physical Properties of the Equilibrium Forms of Cytochrome b5 Revealed Through High Resolution NMR Structures and Backbone Dynamic Analyses"  Biochemistry 37, 8289-8302 (1998).

Assembly members:
cytochrome b5, polymer, 94 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:   Common Name: rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus rattus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cytochrome b5: DKDVKYYTLEEIQKHKDSKS TWVILHHKVYDLTKFLEEHP GGEEVLREQAGGDATENFED VGHSTDARELSKTYIIGELH PDDRSKIAKPSETL

Data sets:
Data typeCount
1H chemical shifts514
15N chemical shifts90
13C chemical shifts297

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb5_B1
2heme2

Entities:

Entity 1, cytb5_B 94 residues - Formula weight is not available

1   ASPLYSASPVALLYSTYRTYRTHRLEUGLU
2   GLUILEGLNLYSHISLYSASPSERLYSSER
3   THRTRPVALILELEUHISHISLYSVALTYR
4   ASPLEUTHRLYSPHELEUGLUGLUHISPRO
5   GLYGLYGLUGLUVALLEUARGGLUGLNALA
6   GLYGLYASPALATHRGLUASNPHEGLUASP
7   VALGLYHISSERTHRASPALAARGGLULEU
8   SERLYSTHRTYRILEILEGLYGLULEUHIS
9   PROASPASPARGSERLYSILEALALYSPRO
10   SERGLUTHRLEU

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: rat ferrocytochrome b5 B form, [U-15N,; U-13C], 4 mM; phosphate 100 mM

sample_conditions_one: ionic strength: 100 .; pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker DMX600 600 MHz

Related Database Links:

BMRB 4130
PDB
DBJ BAA02492 BAB22093 BAB28714 BAE20982
GB AAA67175 AAA72420 AAA72421 AAA72557 AAA99718
REF NP_001268328 NP_071581 NP_080073 XP_003512427 XP_005356388
SP P00173 P56395

Download simulated HSQC data in one of the following formats:
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