BMRB Entry 5680
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5680
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Title: Letter to the Editor: Sequence-specific assignment and secondary structure determination of the 195-residue complex formed by the Mycobacterium tuberculosis proteins CFP-10 and ESAT-6. Towards an understanding of their role in tuberculosis pathogenesis
Deposition date: 2003-01-31 Original release date: 2004-11-29
Authors: Renshaw, Philip; Veverka, Vaclav; Kelly, Geoff; Frenkiel, Thomas; Williamson, Richard; Gordon, Stephen; Hewinson, R.; Carr, Mark
Citation: Renshaw, Philip; Veverka, Vaclav; Kelly, Geoff; Frenkiel, Thomas; Williamson, Richard; Gordon, Stephen; Hewinson, R.; Carr, Mark. "Letter to the Editor: Sequence-specific assignment and secondary structure determination of the 195-residue complex formed by the Mycobacterium tuberculosis proteins CFP-10 and ESAT-6. " J. Biomol. NMR 30, 225-226 (2004).
Assembly members:
Culture Filtrate Protein of 10 kDa, polymer, 100 residues, 10662 Da.
ESAT-6, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Eubacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis and bovis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Culture Filtrate Protein of 10 kDa: MAEMKTDAATLAQEAGNFER
ISGDLKTQIDQVESTAGSLQ
GQWRGAAGTAAQAAVVRFQE
AANKQKQELDEISTNIRQAG
VQYSRADEEQQQALSSQMGF
ESAT-6: MTEQQWNFAGIEAAASAIQG
NVTSIHSLLDEGKQSLTKLA
AAWGGSGSEAYQGVQQKWDA
TATELNNALQNLARTISEAG
QAMASTEGNVTGMFA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1141 |
| 13C chemical shifts | 533 |
| 15N chemical shifts | 194 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CFP-10 | 1 |
| 2 | ESAT-6 | 2 |
Entities:
Entity 1, CFP-10 100 residues - 10662 Da.
| 1 | MET | ALA | GLU | MET | LYS | THR | ASP | ALA | ALA | THR | |
| 2 | LEU | ALA | GLN | GLU | ALA | GLY | ASN | PHE | GLU | ARG | |
| 3 | ILE | SER | GLY | ASP | LEU | LYS | THR | GLN | ILE | ASP | |
| 4 | GLN | VAL | GLU | SER | THR | ALA | GLY | SER | LEU | GLN | |
| 5 | GLY | GLN | TRP | ARG | GLY | ALA | ALA | GLY | THR | ALA | |
| 6 | ALA | GLN | ALA | ALA | VAL | VAL | ARG | PHE | GLN | GLU | |
| 7 | ALA | ALA | ASN | LYS | GLN | LYS | GLN | GLU | LEU | ASP | |
| 8 | GLU | ILE | SER | THR | ASN | ILE | ARG | GLN | ALA | GLY | |
| 9 | VAL | GLN | TYR | SER | ARG | ALA | ASP | GLU | GLU | GLN | |
| 10 | GLN | GLN | ALA | LEU | SER | SER | GLN | MET | GLY | PHE |
Entity 2, ESAT-6 95 residues - Formula weight is not available
| 1 | MET | THR | GLU | GLN | GLN | TRP | ASN | PHE | ALA | GLY | ||||
| 2 | ILE | GLU | ALA | ALA | ALA | SER | ALA | ILE | GLN | GLY | ||||
| 3 | ASN | VAL | THR | SER | ILE | HIS | SER | LEU | LEU | ASP | ||||
| 4 | GLU | GLY | LYS | GLN | SER | LEU | THR | LYS | LEU | ALA | ||||
| 5 | ALA | ALA | TRP | GLY | GLY | SER | GLY | SER | GLU | ALA | ||||
| 6 | TYR | GLN | GLY | VAL | GLN | GLN | LYS | TRP | ASP | ALA | ||||
| 7 | THR | ALA | THR | GLU | LEU | ASN | ASN | ALA | LEU | GLN | ||||
| 8 | ASN | LEU | ALA | ARG | THR | ILE | SER | GLU | ALA | GLY | ||||
| 9 | GLN | ALA | MET | ALA | SER | THR | GLU | GLY | ASN | VAL | ||||
| 10 | THR | GLY | MET | PHE | ALA |
Samples:
sample_1: Culture Filtrate Protein of 10 kDa, [U-13C; U-15N], 0.9 – 1.5 mM; ESAT-6, [U-13C; U-15N], 0.9 – 1.5 mM
condition_1: pH: 6.5 na; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| 15N/1H HSQC | not available | not available | not available |
| 15N/1H TOCSY-HSQC | not available | not available | not available |
| 15N/1H NOESY-HSQC | not available | not available | not available |
| 13C/1H HCCH-TOCSY | not available | not available | not available |
| 13C/1H HMQC-NOESY | not available | not available | not available |
| 15N/13C/1H HNCACB | not available | not available | not available |
| 15N/13C/1H CBCA(CO)NH | not available | not available | not available |
| 15N/13C/1H HAHB(CBCACO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz
- Bruker AVANCE 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts