REMARK SPARTA+ Protein Chemical Shift Prediction Table REMARK REMARK All chemical shifts are reported in ppm: REMARK REMARK SS_SHIFT is the predicted secondary chemical shift. REMARK SHIFT is the predicted chemical shift. REMARK RC_SHIFT is the random coil chemical shift. REMARK HM_SHIFT is the ring current shift; a correction REMARK of 0.6 times this value is applied. REMARK EF_SHIFT is the adjustment for electric field effect, REMARK applied to proton shifts only. REMARK SIGMA is the estimated prediction error. REMARK REMARK Reference: REMARK Y. Shen and A. Bax: SPARTA+: a modest improvement in empirical NMR REMARK chemical shift prediction by means of an artificial neural network. REMARK J. Biomol. NMR 48, 13-22 (2010). DATA SOURCE 1ef4_1_A DATA FIRST_RESID 1 DATA SEQUENCE MIPVRCLSCG KPVSAYFNEY QRRVADGEDP KDVLDDLGLK RYCCRRMLIS DATA SEQUENCE HVETW VARS RESID RESNAME ATOMNAME SS_SHIFT SHIFT RC_SHIFT HM_SHIFT EF_SHIFT SIGMA FORMAT %4d %4s %4s %9.3f %9.3f %9.3f %9.3f %9.3f %9.3f 1 M HA 0.000 4.338 4.480 -0.236 0.000 0.227 1 M C 0.000 176.260 176.300 -0.066 0.000 1.140 1 M CA 0.000 55.163 55.300 -0.229 0.000 0.988 1 M CB 0.000 32.408 32.600 -0.319 0.000 1.302 2 I N 2.813 123.384 120.570 0.002 0.000 2.775 2 I HA 0.014 4.193 4.170 0.014 0.000 0.290 2 I C -1.263 174.852 176.117 -0.003 0.000 1.203 2 I CA -1.808 59.499 61.300 0.012 0.000 1.433 2 I CB -0.287 37.728 38.000 0.025 0.000 1.354 2 I HN 0.197 8.437 8.210 0.050 0.000 0.579 3 P HA 0.134 4.546 4.420 -0.014 0.000 0.279 3 P C 0.124 177.426 177.300 0.003 0.000 1.282 3 P CA -0.811 62.287 63.100 -0.005 0.000 0.788 3 P CB 1.091 32.790 31.700 -0.002 0.000 1.139 4 V N -4.514 115.400 119.914 -0.001 0.000 0.635 4 V HA -0.546 3.574 4.120 -0.001 0.000 0.092 4 V C 0.721 176.816 176.094 0.003 0.000 1.463 4 V CA 2.929 65.231 62.300 0.002 0.000 3.271 4 V CB -1.066 30.762 31.823 0.009 0.000 0.530 4 V HN 0.354 8.541 8.190 -0.005 0.000 0.535 5 R N -0.624 119.882 120.500 0.011 0.000 3.127 5 R HA -0.164 4.183 4.340 0.011 0.000 0.290 5 R C -0.145 176.157 176.300 0.004 0.000 1.089 5 R CA 0.733 56.841 56.100 0.014 0.000 1.188 5 R CB 0.438 30.756 30.300 0.030 0.000 1.175 5 R HN -0.041 8.202 8.270 0.016 0.036 0.550 6 C N 1.922 121.225 119.300 0.004 0.000 2.203 6 C HA 0.116 4.569 4.460 -0.013 0.000 0.325 6 C C -1.826 173.170 174.990 0.010 0.000 1.156 6 C CA -0.156 58.858 59.018 -0.006 0.000 1.597 6 C CB -1.717 26.008 27.740 -0.024 0.000 2.148 6 C HN 0.654 8.890 8.230 0.010 0.000 0.472 7 L N 6.630 127.858 121.223 0.008 0.000 3.059 7 L HA 0.400 4.779 4.340 0.065 0.000 0.298 7 L C -1.550 175.311 176.870 -0.015 0.000 1.304 7 L CA -0.961 53.898 54.840 0.033 0.000 0.855 7 L CB -1.651 40.444 42.059 0.060 0.000 1.266 7 L HN 0.413 8.638 8.230 -0.008 0.000 0.572 8 S N -1.418 114.272 115.700 -0.017 0.000 5.361 8 S HA -0.003 4.515 4.470 -0.028 -0.064 0.140 8 S C 0.344 174.928 174.600 -0.026 0.000 1.100 8 S CA 0.682 58.864 58.200 -0.031 0.000 1.373 8 S CB 1.246 64.416 63.200 -0.049 0.000 1.803 8 S HN -0.728 7.576 8.310 -0.010 0.000 0.495 9 C N -1.212 118.071 119.300 -0.028 0.000 4.392 9 C HA -0.193 4.247 4.460 -0.033 0.000 0.280 9 C C 0.086 175.060 174.990 -0.027 0.000 1.381 9 C CA 1.198 60.197 59.018 -0.031 0.000 1.871 9 C CB -2.485 25.232 27.740 -0.038 0.000 1.323 9 C HN 0.472 8.685 8.230 -0.028 0.000 0.772 10 G N -5.071 103.715 108.800 -0.023 0.000 3.006 10 G HA2 -0.138 3.811 3.960 -0.017 0.000 0.195 10 G HA3 -0.138 3.812 3.960 -0.017 0.000 0.195 10 G C -1.764 173.128 174.900 -0.013 0.000 1.034 10 G CA -0.113 44.977 45.100 -0.018 0.000 0.807 10 G HN 0.059 8.276 8.290 -0.026 0.058 0.469 11 K N 2.468 122.860 120.400 -0.013 0.000 2.270 11 K HA 0.564 4.889 4.320 0.008 0.000 0.255 11 K C -2.949 173.650 176.600 -0.002 0.000 0.936 11 K CA -3.540 52.748 56.287 0.001 0.000 0.809 11 K CB 1.156 33.659 32.500 0.006 0.000 1.131 11 K HN -0.629 7.521 8.250 -0.019 0.089 0.427 12 P HA -0.045 4.331 4.420 -0.073 0.000 0.265 12 P C -0.800 176.500 177.300 -0.000 0.000 1.193 12 P CA 0.102 63.187 63.100 -0.026 0.000 0.765 12 P CB 0.806 32.494 31.700 -0.020 0.000 0.823 13 V N -3.348 116.473 119.914 -0.156 0.000 3.621 13 V HA 0.182 4.341 4.120 0.066 0.000 0.263 13 V C 0.741 176.491 176.094 -0.572 0.000 1.272 13 V CA -0.312 61.860 62.300 -0.213 0.000 1.080 13 V CB 1.056 32.748 31.823 -0.218 0.000 0.816 13 V HN -0.344 7.703 8.190 -0.239 0.000 0.451 14 S N 3.399 118.726 115.700 -0.622 0.000 2.351 14 S HA -0.399 3.511 4.470 -0.934 0.000 0.220 14 S C 1.916 176.422 174.600 -0.155 0.000 1.035 14 S CA 3.810 61.691 58.200 -0.531 0.000 1.031 14 S CB -1.283 61.729 63.200 -0.313 0.000 0.928 14 S HN 0.276 8.313 8.310 -0.455 0.000 0.433 15 A N 0.729 123.483 122.820 -0.110 0.000 1.978 15 A HA -0.196 4.072 4.320 -0.086 0.000 0.220 15 A C 1.155 178.680 177.584 -0.098 0.000 1.170 15 A CA 2.507 54.479 52.037 -0.109 0.000 0.636 15 A CB -0.628 18.270 19.000 -0.170 0.000 0.810 15 A HN 0.342 8.416 8.150 -0.126 0.000 0.448 16 Y N -4.155 116.168 120.300 0.037 0.000 2.448 16 Y HA -0.037 4.572 4.550 0.098 0.000 0.289 16 Y C 1.629 177.687 175.900 0.263 0.000 1.114 16 Y CA 1.620 59.794 58.100 0.123 0.000 1.235 16 Y CB 0.378 38.905 38.460 0.113 0.000 1.045 16 Y HN -0.677 7.576 8.280 0.157 0.121 0.554 17 F N 0.705 120.755 119.950 0.166 0.000 2.225 17 F HA -0.505 4.096 4.527 0.125 0.000 0.302 17 F C 1.812 177.702 175.800 0.150 0.000 1.068 17 F CA 2.084 60.156 58.000 0.120 0.000 1.327 17 F CB -1.404 37.621 39.000 0.041 0.000 1.043 17 F HN 0.628 8.938 8.300 0.373 0.213 0.506 18 N N -0.984 117.887 118.700 0.284 0.000 2.166 18 N HA -0.393 4.439 4.740 0.153 0.000 0.186 18 N C 2.018 177.623 175.510 0.159 0.000 1.019 18 N CA 3.970 57.119 53.050 0.166 0.000 0.856 18 N CB -0.295 38.242 38.487 0.082 0.000 0.993 18 N HN -0.381 8.112 8.380 0.255 0.040 0.426 19 E N 0.915 121.234 120.200 0.199 0.000 2.031 19 E HA -0.310 4.111 4.350 0.118 0.000 0.193 19 E C 1.796 178.497 176.600 0.169 0.000 0.994 19 E CA 3.142 59.650 56.400 0.180 0.000 0.800 19 E CB -0.356 29.495 29.700 0.253 0.000 0.752 19 E HN 0.138 8.514 8.360 0.235 0.125 0.447 20 Y N 0.237 120.593 120.300 0.093 0.000 2.114 20 Y HA -0.525 4.042 4.550 0.029 0.000 0.282 20 Y C 1.590 177.492 175.900 0.004 0.000 1.165 20 Y CA 3.694 61.810 58.100 0.027 0.000 1.148 20 Y CB -0.072 38.367 38.460 -0.035 0.000 0.972 20 Y HN -0.772 7.737 8.280 0.381 0.000 0.504 21 Q N -0.848 119.072 119.800 0.201 0.000 2.002 21 Q HA -0.541 3.847 4.340 0.080 0.000 0.204 21 Q C 2.041 178.029 176.000 -0.021 0.000 0.988 21 Q CA 3.846 59.702 55.803 0.089 0.000 0.843 21 Q CB -0.104 28.709 28.738 0.125 0.000 0.908 21 Q HN -0.010 8.447 8.270 0.312 0.000 0.420 22 R N -1.334 119.172 120.500 0.010 0.000 2.094 22 R HA -0.442 3.893 4.340 -0.008 0.000 0.239 22 R C 2.412 178.686 176.300 -0.045 0.000 1.137 22 R CA 3.607 59.702 56.100 -0.008 0.000 0.943 22 R CB -0.123 30.184 30.300 0.013 0.000 0.850 22 R HN 0.047 8.349 8.270 0.052 0.000 0.433 23 R N -1.566 118.896 120.500 -0.064 0.000 2.075 23 R HA -0.296 4.009 4.340 -0.058 0.000 0.230 23 R C 2.528 178.734 176.300 -0.156 0.000 1.140 23 R CA 3.277 59.321 56.100 -0.094 0.000 0.928 23 R CB -0.384 29.862 30.300 -0.089 0.000 0.834 23 R HN 0.100 8.347 8.270 -0.039 0.000 0.429 24 V N -0.317 119.415 119.914 -0.303 0.000 2.439 24 V HA -0.465 3.486 4.120 -0.282 0.000 0.253 24 V C 2.068 178.061 176.094 -0.170 0.000 1.074 24 V CA 4.084 66.182 62.300 -0.337 0.000 1.076 24 V CB -0.162 31.262 31.823 -0.664 0.000 0.664 24 V HN 0.264 8.108 8.190 -0.407 0.101 0.461 25 A N -0.412 122.335 122.820 -0.120 0.000 1.845 25 A HA -0.307 3.989 4.320 -0.040 0.000 0.215 25 A C 1.387 178.945 177.584 -0.043 0.000 1.195 25 A CA 2.815 54.819 52.037 -0.055 0.000 0.616 25 A CB -0.594 18.387 19.000 -0.031 0.000 0.832 25 A HN -0.045 7.900 8.150 -0.135 0.124 0.443 26 D N -2.480 117.893 120.400 -0.045 0.000 2.219 26 D HA -0.097 4.529 4.640 -0.023 0.000 0.205 26 D C 1.226 177.504 176.300 -0.036 0.000 0.970 26 D CA 1.563 55.543 54.000 -0.032 0.000 0.851 26 D CB 0.820 41.603 40.800 -0.027 0.000 0.943 26 D HN -0.533 7.806 8.370 -0.052 0.000 0.488 27 G N -2.215 106.553 108.800 -0.054 0.000 2.157 27 G HA2 -0.305 3.619 3.960 -0.060 0.000 0.118 27 G HA3 -0.305 3.633 3.960 -0.036 0.000 0.118 27 G C -0.988 173.879 174.900 -0.054 0.000 1.032 27 G CA -0.434 44.635 45.100 -0.051 0.000 0.697 27 G HN -0.113 8.029 8.290 -0.073 0.105 0.495 28 E N 0.222 120.380 120.200 -0.070 0.000 2.313 28 E HA 0.046 4.372 4.350 -0.040 0.000 0.272 28 E C -0.520 176.037 176.600 -0.072 0.000 1.038 28 E CA -0.744 55.621 56.400 -0.059 0.000 0.863 28 E CB 1.034 30.703 29.700 -0.052 0.000 1.060 28 E HN -0.180 8.015 8.360 -0.086 0.113 0.402 29 D N 4.339 124.714 120.400 -0.043 0.000 2.402 29 D HA -0.039 4.581 4.640 -0.035 0.000 0.268 29 D C -0.289 175.991 176.300 -0.033 0.000 1.294 29 D CA -0.446 53.535 54.000 -0.032 0.000 0.945 29 D CB 0.248 41.041 40.800 -0.012 0.000 1.112 29 D HN 0.425 8.777 8.370 -0.030 0.000 0.517 30 P HA -0.237 4.127 4.420 -0.092 0.000 0.215 30 P C 1.272 178.625 177.300 0.087 0.000 1.153 30 P CA 2.174 65.264 63.100 -0.017 0.000 0.853 30 P CB 0.264 31.978 31.700 0.024 0.000 0.788 31 K N -3.924 116.517 120.400 0.068 0.000 2.113 31 K HA -0.385 3.984 4.320 0.081 0.000 0.208 31 K C 2.065 178.694 176.600 0.048 0.000 1.047 31 K CA 3.799 60.123 56.287 0.062 0.000 0.928 31 K CB -0.573 31.949 32.500 0.037 0.000 0.716 31 K HN 0.285 8.564 8.250 0.049 0.000 0.446 32 D N -0.790 119.630 120.400 0.033 0.000 2.117 32 D HA -0.202 4.451 4.640 0.023 0.000 0.198 32 D C 2.374 178.698 176.300 0.039 0.000 0.982 32 D CA 3.421 57.437 54.000 0.026 0.000 0.828 32 D CB -0.038 40.770 40.800 0.015 0.000 0.967 32 D HN -0.587 7.674 8.370 0.024 0.124 0.464 33 V N 1.126 121.073 119.914 0.056 0.000 2.343 33 V HA -0.441 3.726 4.120 0.078 0.000 0.247 33 V C 2.135 178.285 176.094 0.093 0.000 1.051 33 V CA 3.379 65.733 62.300 0.089 0.000 1.036 33 V CB -0.127 31.770 31.823 0.125 0.000 0.654 33 V HN -0.906 7.201 8.190 0.047 0.111 0.451 34 L N -1.186 120.097 121.223 0.101 0.000 2.089 34 L HA -0.420 3.967 4.340 0.077 0.000 0.213 34 L C 2.174 179.047 176.870 0.006 0.000 1.079 34 L CA 3.683 58.563 54.840 0.066 0.000 0.758 34 L CB -1.914 40.189 42.059 0.073 0.000 0.891 34 L HN 0.664 8.779 8.230 0.117 0.185 0.433 35 D N -2.556 117.849 120.400 0.010 0.000 2.144 35 D HA -0.263 4.367 4.640 -0.018 0.000 0.199 35 D C 2.097 178.366 176.300 -0.051 0.000 0.984 35 D CA 3.363 57.355 54.000 -0.013 0.000 0.834 35 D CB 0.087 40.889 40.800 0.004 0.000 0.955 35 D HN -0.525 7.743 8.370 0.029 0.119 0.465 36 D N -1.243 119.138 120.400 -0.032 0.000 2.137 36 D HA -0.162 4.459 4.640 -0.032 0.000 0.202 36 D C 2.396 178.514 176.300 -0.304 0.000 0.970 36 D CA 2.484 56.459 54.000 -0.042 0.000 0.837 36 D CB 0.682 41.531 40.800 0.081 0.000 0.981 36 D HN -0.290 7.957 8.370 0.007 0.127 0.475 37 L N -1.519 119.558 121.223 -0.244 0.000 2.051 37 L HA -0.388 3.684 4.340 -0.446 0.000 0.214 37 L C 0.833 177.114 176.870 -0.982 0.000 1.076 37 L CA 2.763 57.314 54.840 -0.481 0.000 0.758 37 L CB 0.646 42.694 42.059 -0.017 0.000 0.890 37 L HN 0.400 8.469 8.230 -0.051 0.130 0.433 38 G N -3.967 104.539 108.800 -0.490 0.000 2.452 38 G HA2 -0.339 3.488 3.960 -0.221 0.000 0.275 38 G HA3 -0.339 3.340 3.960 -0.468 0.000 0.275 38 G C -0.558 174.231 174.900 -0.185 0.000 1.131 38 G CA -0.267 44.623 45.100 -0.350 0.000 1.031 38 G HN -0.550 7.554 8.290 -0.298 0.007 0.511 39 L N -1.132 120.032 121.223 -0.099 0.000 2.335 39 L HA 0.271 4.586 4.340 -0.041 0.000 0.268 39 L C -0.362 176.495 176.870 -0.021 0.000 1.037 39 L CA -1.420 53.398 54.840 -0.038 0.000 0.895 39 L CB -0.358 41.706 42.059 0.008 0.000 1.266 39 L HN -0.706 7.469 8.230 -0.091 0.000 0.439 40 K N 2.458 122.842 120.400 -0.027 0.000 2.561 40 K HA -0.250 4.063 4.320 -0.012 0.000 0.280 40 K C -0.103 176.483 176.600 -0.024 0.000 0.975 40 K CA 0.743 57.018 56.287 -0.020 0.000 1.024 40 K CB 0.329 32.815 32.500 -0.023 0.000 0.883 40 K HN 0.265 8.495 8.250 -0.034 0.000 0.496 41 R N -2.184 118.305 120.500 -0.019 0.000 1.168 41 R HA -0.335 3.980 4.340 -0.041 0.000 0.418 41 R C 1.105 177.374 176.300 -0.052 0.000 1.353 41 R CA 0.872 56.931 56.100 -0.069 0.000 1.272 41 R CB -0.722 29.496 30.300 -0.137 0.000 3.598 41 R HN 0.234 8.504 8.270 0.001 0.000 0.493 42 Y N -1.684 118.619 120.300 0.004 0.000 2.571 42 Y HA -0.069 4.485 4.550 0.008 0.000 0.294 42 Y C 1.193 177.099 175.900 0.011 0.000 1.141 42 Y CA 1.812 59.917 58.100 0.008 0.000 1.308 42 Y CB -0.796 37.669 38.460 0.008 0.000 1.002 42 Y HN 0.331 8.605 8.280 -0.010 0.000 0.551 43 C N -3.433 115.561 119.300 -0.510 0.000 2.398 43 C HA -0.330 3.956 4.460 -0.290 0.000 0.282 43 C C 1.219 176.168 174.990 -0.068 0.000 1.275 43 C CA 0.713 59.547 59.018 -0.308 0.000 1.797 43 C CB -1.954 25.601 27.740 -0.309 0.000 1.991 43 C HN 0.182 7.927 8.230 -0.724 0.051 0.505 44 C N 1.234 120.513 119.300 -0.035 0.000 2.602 44 C HA 0.183 4.821 4.460 0.016 -0.169 0.282 44 C C 2.298 177.319 174.990 0.052 0.000 1.313 44 C CA 1.137 60.164 59.018 0.016 0.000 1.699 44 C CB -0.346 27.404 27.740 0.015 0.000 2.124 44 C HN -0.724 7.433 8.230 -0.061 0.036 0.509 45 R N 1.142 121.684 120.500 0.070 0.000 2.115 45 R HA -0.503 3.864 4.340 0.044 0.000 0.239 45 R C 2.531 178.882 176.300 0.085 0.000 1.133 45 R CA 3.545 59.691 56.100 0.076 0.000 0.935 45 R CB -0.530 29.831 30.300 0.102 0.000 0.853 45 R HN -0.276 8.034 8.270 0.066 0.000 0.433 46 R N -2.586 118.001 120.500 0.145 0.000 2.174 46 R HA -0.357 4.051 4.340 0.113 0.000 0.253 46 R C 2.132 178.488 176.300 0.094 0.000 1.165 46 R CA 2.921 59.105 56.100 0.140 0.000 0.984 46 R CB -1.126 29.314 30.300 0.233 0.000 0.873 46 R HN 0.325 8.615 8.270 0.207 0.104 0.456 47 M N -2.162 117.487 119.600 0.082 0.000 2.357 47 M HA -0.171 4.364 4.480 0.092 0.000 0.266 47 M C 2.317 178.648 176.300 0.051 0.000 1.095 47 M CA 3.070 58.417 55.300 0.079 0.000 1.156 47 M CB 0.367 33.013 32.600 0.077 0.000 1.365 47 M HN -0.297 7.896 8.290 0.082 0.146 0.447 48 L N -0.413 120.808 121.223 -0.003 0.000 1.982 48 L HA -0.286 4.020 4.340 -0.179 -0.074 0.206 48 L C 2.217 178.980 176.870 -0.179 0.000 1.078 48 L CA 2.983 57.742 54.840 -0.135 0.000 0.749 48 L CB -0.626 41.349 42.059 -0.140 0.000 0.894 48 L HN 0.156 8.194 8.230 0.023 0.205 0.436 49 I N -5.057 115.462 120.570 -0.086 0.000 2.290 49 I HA -0.586 3.631 4.170 0.078 0.000 0.253 49 I C 1.710 177.874 176.117 0.079 0.000 1.112 49 I CA 3.038 64.361 61.300 0.038 0.000 1.377 49 I CB -0.583 37.477 38.000 0.101 0.000 1.060 49 I HN -0.137 7.954 8.210 -0.042 0.094 0.428 50 S N -1.184 114.549 115.700 0.054 0.000 2.339 50 S HA -0.172 4.347 4.470 0.083 0.000 0.213 50 S C 0.335 175.002 174.600 0.112 0.000 1.033 50 S CA 3.086 61.333 58.200 0.079 0.000 0.950 50 S CB 0.510 63.751 63.200 0.069 0.000 0.893 50 S HN -0.552 7.743 8.310 0.039 0.038 0.492 51 H N 1.053 120.105 119.070 -0.029 0.000 2.273 51 H HA -0.016 4.531 4.556 -0.015 0.000 0.317 51 H C 0.839 176.114 175.328 -0.088 0.000 1.062 51 H CA 0.348 56.376 56.048 -0.034 0.000 1.419 51 H CB 2.156 31.908 29.762 -0.017 0.000 1.442 51 H HN -0.250 8.017 8.280 0.153 0.105 0.542 52 V N 0.670 120.429 119.914 -0.259 0.000 5.562 52 V HA -0.332 3.542 4.120 -0.411 0.000 0.232 52 V C -1.865 173.973 176.094 -0.426 0.000 0.694 52 V CA 0.084 62.098 62.300 -0.477 0.000 0.574 52 V CB -0.375 30.975 31.823 -0.788 0.000 0.230 52 V HN -0.570 7.539 8.190 -0.136 0.000 0.568 53 E N 1.888 121.761 120.200 -0.545 0.000 2.351 53 E HA -0.239 4.092 4.350 -0.031 0.000 0.241 53 E C 0.577 177.162 176.600 -0.026 0.000 1.243 53 E CA 0.987 57.264 56.400 -0.206 0.000 0.963 53 E CB -0.721 28.863 29.700 -0.193 0.000 1.042 53 E HN 0.130 7.831 8.360 -1.099 0.000 0.468 54 T N 1.405 115.976 114.554 0.029 0.000 2.985 54 T HA -0.169 4.240 4.350 0.098 0.000 0.266 54 T C 0.467 175.261 174.700 0.156 0.000 1.076 54 T CA 0.645 62.794 62.100 0.081 0.000 1.135 54 T CB 0.230 69.128 68.868 0.050 0.000 0.890 54 T HN 0.008 8.269 8.240 0.035 0.000 0.480 55 W N 0.000 121.314 121.300 0.024 0.000 2.388 55 W HA 0.000 4.682 4.660 0.037 0.000 0.303 55 W CA 0.000 57.365 57.345 0.034 0.000 1.226 55 W CB 0.000 29.481 29.460 0.036 0.000 1.126 55 W HN 0.000 8.418 8.180 0.432 0.022 0.535