REMARK SPARTA+ Protein Chemical Shift Prediction Table REMARK REMARK All chemical shifts are reported in ppm: REMARK REMARK SS_SHIFT is the predicted secondary chemical shift. REMARK SHIFT is the predicted chemical shift. REMARK RC_SHIFT is the random coil chemical shift. REMARK HM_SHIFT is the ring current shift; a correction REMARK of 0.6 times this value is applied. REMARK EF_SHIFT is the adjustment for electric field effect, REMARK applied to proton shifts only. REMARK SIGMA is the estimated prediction error. REMARK REMARK Reference: REMARK Y. Shen and A. Bax: SPARTA+: a modest improvement in empirical NMR REMARK chemical shift prediction by means of an artificial neural network. REMARK J. Biomol. NMR 48, 13-22 (2010). DATA SOURCE 2oow_1_C DATA FIRST_RESID 1 DATA SEQUENCE PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG DATA SEQUENCE GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD DATA SEQUENCE MNAANVGWNN STFA VARS RESID RESNAME ATOMNAME SS_SHIFT SHIFT RC_SHIFT HM_SHIFT EF_SHIFT SIGMA FORMAT %4d %4s %4s %9.3f %9.3f %9.3f %9.3f %9.3f %9.3f 1 P HA 0.000 nan 4.420 nan 0.000 0.216 1 P C 0.000 177.347 177.300 0.078 0.000 1.155 1 P CA 0.000 63.112 63.100 0.020 0.000 0.800 1 P CB 0.000 31.687 31.700 -0.022 0.000 0.726 2 M N 2.362 122.046 119.600 0.140 0.000 2.204 2 M HA 0.652 5.133 4.480 0.002 0.000 0.293 2 M C -2.022 174.444 176.300 0.277 0.000 0.994 2 M CA -0.650 54.751 55.300 0.168 0.000 0.925 2 M CB 1.563 34.227 32.600 0.106 0.000 1.577 2 M HN 0.396 nan 8.290 nan 0.000 0.439 3 F N 6.699 126.688 119.950 0.065 0.000 2.493 3 F HA 0.714 5.250 4.527 0.015 0.000 0.329 3 F C -1.712 174.129 175.800 0.069 0.000 1.126 3 F CA -0.871 57.159 58.000 0.050 0.000 0.937 3 F CB 1.066 40.059 39.000 -0.012 0.000 1.146 3 F HN 0.483 nan 8.300 nan 0.000 0.442 4 I N 6.571 126.817 120.570 -0.539 0.000 2.509 4 I HA 0.528 4.699 4.170 0.002 0.000 0.293 4 I C -1.216 174.456 176.117 -0.741 0.000 1.020 4 I CA -1.148 59.862 61.300 -0.482 0.000 1.088 4 I CB 2.076 39.953 38.000 -0.204 0.000 1.267 4 I HN 0.236 nan 8.210 nan 0.000 0.430 5 V N 5.472 125.053 119.914 -0.555 0.000 2.483 5 V HA 0.404 4.526 4.120 0.002 0.000 0.297 5 V C -0.456 175.513 176.094 -0.208 0.000 1.027 5 V CA -0.715 61.371 62.300 -0.357 0.000 0.855 5 V CB 1.762 33.479 31.823 -0.176 0.000 0.995 5 V HN 0.670 nan 8.190 nan 0.000 0.424 6 N N 2.428 121.043 118.700 -0.140 0.000 2.392 6 N HA 0.617 5.358 4.740 0.002 0.000 0.283 6 N C -0.628 174.862 175.510 -0.034 0.000 1.003 6 N CA -0.180 52.814 53.050 -0.095 0.000 0.892 6 N CB 2.475 40.915 38.487 -0.079 0.000 1.193 6 N HN 0.699 nan 8.380 nan 0.000 0.487 7 T N 0.008 114.548 114.554 -0.023 0.000 2.868 7 T HA 0.191 4.542 4.350 0.002 0.000 0.306 7 T C 0.280 174.976 174.700 -0.007 0.000 1.224 7 T CA -0.690 61.406 62.100 -0.007 0.000 1.012 7 T CB 0.779 69.642 68.868 -0.007 0.000 1.221 7 T HN 0.562 nan 8.240 nan 0.000 0.499 8 N N 1.951 120.649 118.700 -0.003 0.000 2.398 8 N HA 0.058 4.799 4.740 0.002 0.000 0.188 8 N C 0.447 175.948 175.510 -0.014 0.000 1.122 8 N CA 0.003 53.050 53.050 -0.004 0.000 0.866 8 N CB -0.313 38.176 38.487 0.003 0.000 0.970 8 N HN 0.311 nan 8.380 nan 0.000 0.462 9 V N 2.393 122.293 119.914 -0.023 0.000 2.655 9 V HA 0.122 4.244 4.120 0.002 0.000 0.300 9 V C -1.945 174.131 176.094 -0.030 0.000 1.044 9 V CA -1.011 61.269 62.300 -0.035 0.000 1.095 9 V CB 0.606 32.396 31.823 -0.055 0.000 0.952 9 V HN 0.131 nan 8.190 nan 0.000 0.485 10 P HA 0.116 nan 4.420 nan 0.000 0.269 10 P C 0.740 178.025 177.300 -0.023 0.000 1.209 10 P CA -0.233 62.854 63.100 -0.021 0.000 0.776 10 P CB 0.452 32.139 31.700 -0.020 0.000 0.876 11 R N 3.131 123.624 120.500 -0.011 0.000 2.103 11 R HA -0.207 4.135 4.340 0.002 0.000 0.242 11 R C 1.721 178.015 176.300 -0.011 0.000 1.142 11 R CA 2.206 58.304 56.100 -0.004 0.000 0.960 11 R CB -0.994 29.311 30.300 0.009 0.000 0.858 11 R HN 0.559 nan 8.270 nan 0.000 0.439 12 A N -0.252 122.560 122.820 -0.013 0.000 2.125 12 A HA -0.067 4.255 4.320 0.002 0.000 0.219 12 A C 1.967 179.534 177.584 -0.029 0.000 1.156 12 A CA 1.538 53.566 52.037 -0.015 0.000 0.671 12 A CB -0.150 18.843 19.000 -0.012 0.000 0.794 12 A HN 0.354 nan 8.150 nan 0.000 0.459 13 S N -0.773 114.900 115.700 -0.044 0.000 2.593 13 S HA 0.155 4.627 4.470 0.002 0.000 0.217 13 S C 0.374 174.906 174.600 -0.113 0.000 0.966 13 S CA -0.083 58.076 58.200 -0.068 0.000 0.914 13 S CB 0.052 63.211 63.200 -0.069 0.000 0.776 13 S HN 0.224 nan 8.310 nan 0.000 0.523 14 V N 5.516 125.368 119.914 -0.104 0.000 2.389 14 V HA 0.238 4.359 4.120 0.002 0.000 0.264 14 V C -1.902 174.137 176.094 -0.091 0.000 1.049 14 V CA -2.033 60.169 62.300 -0.163 0.000 0.932 14 V CB 0.441 32.220 31.823 -0.074 0.000 1.011 14 V HN 0.218 nan 8.190 nan 0.000 0.475 15 P HA 0.159 nan 4.420 nan 0.000 0.271 15 P C -0.543 176.796 177.300 0.065 0.000 1.218 15 P CA -0.423 62.665 63.100 -0.020 0.000 0.780 15 P CB 0.831 32.514 31.700 -0.027 0.000 0.901 16 D N 0.894 121.329 120.400 0.059 0.000 2.443 16 D HA 0.286 4.927 4.640 0.002 0.000 0.239 16 D C 1.616 177.979 176.300 0.105 0.000 1.136 16 D CA 1.668 55.715 54.000 0.078 0.000 0.879 16 D CB -0.004 40.827 40.800 0.051 0.000 1.195 16 D HN 0.701 nan 8.370 nan 0.000 0.443 17 G N 1.639 110.510 108.800 0.118 0.000 2.175 17 G HA2 -0.360 3.601 3.960 0.002 0.000 0.244 17 G HA3 -0.360 3.601 3.960 0.002 0.000 0.244 17 G C 0.939 175.941 174.900 0.170 0.000 0.982 17 G CA 0.230 45.398 45.100 0.115 0.000 0.641 17 G HN 0.466 nan 8.290 nan 0.000 0.527 18 F N 1.226 121.199 119.950 0.039 0.000 2.134 18 F HA 0.136 4.670 4.527 0.012 0.000 0.299 18 F C 2.524 178.364 175.800 0.065 0.000 1.097 18 F CA 2.104 60.134 58.000 0.051 0.000 1.264 18 F CB -0.372 38.660 39.000 0.053 0.000 1.001 18 F HN 0.220 nan 8.300 nan 0.000 0.479 19 L N -1.063 120.173 121.223 0.022 0.000 2.083 19 L HA -0.223 4.118 4.340 0.002 0.000 0.209 19 L C 2.509 179.337 176.870 -0.069 0.000 1.083 19 L CA 1.413 56.210 54.840 -0.072 0.000 0.752 19 L CB -0.936 41.140 42.059 0.028 0.000 0.899 19 L HN 0.053 nan 8.230 nan 0.000 0.433 20 S N -0.566 115.125 115.700 -0.015 0.000 2.383 20 S HA -0.223 4.249 4.470 0.002 0.000 0.227 20 S C 1.868 176.449 174.600 -0.032 0.000 1.026 20 S CA 1.364 59.558 58.200 -0.010 0.000 0.981 20 S CB -0.104 63.106 63.200 0.016 0.000 0.818 20 S HN 0.431 nan 8.310 nan 0.000 0.472 21 E N 1.050 121.227 120.200 -0.039 0.000 2.077 21 E HA -0.111 4.241 4.350 0.002 0.000 0.193 21 E C 1.966 178.501 176.600 -0.110 0.000 0.989 21 E CA 0.856 57.230 56.400 -0.043 0.000 0.800 21 E CB -0.162 29.547 29.700 0.017 0.000 0.746 21 E HN 0.408 nan 8.360 nan 0.000 0.452 22 L N 0.458 121.545 121.223 -0.226 0.000 2.046 22 L HA -0.189 4.152 4.340 0.002 0.000 0.208 22 L C 2.664 179.456 176.870 -0.131 0.000 1.077 22 L CA 1.645 56.344 54.840 -0.235 0.000 0.747 22 L CB -0.612 41.269 42.059 -0.297 0.000 0.896 22 L HN 0.248 nan 8.230 nan 0.000 0.432 23 T N -1.164 113.339 114.554 -0.084 0.000 2.652 23 T HA -0.272 4.079 4.350 0.002 0.000 0.267 23 T C 1.858 176.535 174.700 -0.038 0.000 1.039 23 T CA 1.442 63.517 62.100 -0.041 0.000 1.153 23 T CB -0.231 68.626 68.868 -0.019 0.000 0.863 23 T HN 0.315 nan 8.240 nan 0.000 0.428 24 Q N 0.543 120.322 119.800 -0.035 0.000 2.050 24 Q HA -0.165 4.177 4.340 0.002 0.000 0.202 24 Q C 2.321 178.304 176.000 -0.029 0.000 0.980 24 Q CA 1.434 57.224 55.803 -0.022 0.000 0.840 24 Q CB -0.215 28.515 28.738 -0.013 0.000 0.898 24 Q HN 0.457 nan 8.270 nan 0.000 0.424 25 Q N 0.205 119.977 119.800 -0.046 0.000 2.124 25 Q HA -0.062 4.279 4.340 0.002 0.000 0.202 25 Q C 2.352 178.318 176.000 -0.056 0.000 0.977 25 Q CA 0.905 56.681 55.803 -0.047 0.000 0.850 25 Q CB -0.223 28.478 28.738 -0.062 0.000 0.901 25 Q HN 0.423 nan 8.270 nan 0.000 0.429 26 L N -0.084 121.084 121.223 -0.092 0.000 2.109 26 L HA -0.108 4.234 4.340 0.002 0.000 0.207 26 L C 2.417 179.255 176.870 -0.053 0.000 1.086 26 L CA 0.848 55.621 54.840 -0.110 0.000 0.760 26 L CB -0.547 41.420 42.059 -0.152 0.000 0.910 26 L HN 0.104 nan 8.230 nan 0.000 0.437 27 A N -0.406 122.395 122.820 -0.032 0.000 1.902 27 A HA -0.286 4.035 4.320 0.002 0.000 0.217 27 A C 2.259 179.842 177.584 -0.002 0.000 1.181 27 A CA 1.875 53.905 52.037 -0.011 0.000 0.623 27 A CB -0.551 18.449 19.000 -0.001 0.000 0.818 27 A HN 0.477 nan 8.150 nan 0.000 0.443 28 Q N -0.633 119.166 119.800 -0.001 0.000 2.050 28 Q HA -0.110 4.232 4.340 0.002 0.000 0.202 28 Q C 2.204 178.219 176.000 0.026 0.000 0.980 28 Q CA 1.589 57.397 55.803 0.007 0.000 0.840 28 Q CB -0.332 28.408 28.738 0.003 0.000 0.898 28 Q HN 0.591 nan 8.270 nan 0.000 0.424 29 A N 0.244 123.094 122.820 0.049 0.000 1.898 29 A HA -0.163 4.159 4.320 0.002 0.000 0.216 29 A C 2.208 179.919 177.584 0.211 0.000 1.181 29 A CA 2.006 54.123 52.037 0.133 0.000 0.620 29 A CB -0.932 18.196 19.000 0.213 0.000 0.819 29 A HN 0.652 nan 8.150 nan 0.000 0.442 30 T N -4.675 109.966 114.554 0.144 0.000 3.054 30 T HA 0.370 4.722 4.350 0.002 0.000 0.259 30 T C 1.461 176.189 174.700 0.047 0.000 1.092 30 T CA 1.194 63.371 62.100 0.128 0.000 1.121 30 T CB -0.052 68.821 68.868 0.009 0.000 0.912 30 T HN 1.748 nan 8.240 nan 0.000 0.489 31 G N 1.351 110.163 108.800 0.019 0.000 2.136 31 G HA2 -0.219 3.743 3.960 0.002 0.000 0.242 31 G HA3 -0.219 3.743 3.960 0.002 0.000 0.242 31 G C -0.132 174.748 174.900 -0.032 0.000 0.989 31 G CA 0.213 45.308 45.100 -0.008 0.000 0.682 31 G HN 0.696 nan 8.290 nan 0.000 0.522 32 K N 0.314 120.692 120.400 -0.038 0.000 2.156 32 K HA 0.541 4.863 4.320 0.002 0.000 0.250 32 K C -2.621 173.977 176.600 -0.005 0.000 0.955 32 K CA -2.216 54.032 56.287 -0.066 0.000 0.855 32 K CB 1.616 34.046 32.500 -0.117 0.000 1.101 32 K HN -0.057 nan 8.250 nan 0.000 0.434 33 P HA -0.014 nan 4.420 nan 0.000 0.268 33 P C -1.971 175.397 177.300 0.114 0.000 1.205 33 P CA -1.022 62.140 63.100 0.103 0.000 0.771 33 P CB 0.189 32.006 31.700 0.195 0.000 0.858 34 P HA -0.184 nan 4.420 nan 0.000 0.221 34 P C 0.988 178.306 177.300 0.030 0.000 1.145 34 P CA 1.431 64.554 63.100 0.038 0.000 0.795 34 P CB 0.139 31.850 31.700 0.019 0.000 0.775 35 Q N -1.319 118.490 119.800 0.016 0.000 2.291 35 Q HA -0.128 4.213 4.340 0.002 0.000 0.206 35 Q C 1.445 177.331 176.000 -0.191 0.000 0.976 35 Q CA 1.326 57.074 55.803 -0.093 0.000 0.875 35 Q CB -0.797 27.841 28.738 -0.168 0.000 0.927 35 Q HN 0.438 nan 8.270 nan 0.000 0.450 36 Y N -0.871 119.390 120.300 -0.065 0.000 2.462 36 Y HA 0.134 4.678 4.550 -0.010 0.000 0.261 36 Y C 0.166 176.021 175.900 -0.075 0.000 1.146 36 Y CA -0.458 57.575 58.100 -0.112 0.000 1.283 36 Y CB 0.765 39.112 38.460 -0.188 0.000 1.090 36 Y HN -0.012 nan 8.280 nan 0.000 0.526 37 I N 1.228 121.846 120.570 0.080 0.000 2.371 37 I HA 0.336 4.508 4.170 0.002 0.000 0.290 37 I C 0.320 176.467 176.117 0.050 0.000 1.028 37 I CA -0.592 60.740 61.300 0.053 0.000 1.345 37 I CB 0.324 38.342 38.000 0.029 0.000 1.407 37 I HN -0.051 nan 8.210 nan 0.000 0.501 38 A N 7.254 130.115 122.820 0.069 0.000 2.342 38 A HA 0.809 5.130 4.320 0.002 0.000 0.323 38 A C -0.466 177.179 177.584 0.103 0.000 1.125 38 A CA -0.495 51.584 52.037 0.071 0.000 0.785 38 A CB 1.682 20.724 19.000 0.070 0.000 1.221 38 A HN 0.577 nan 8.150 nan 0.000 0.463 39 V N 0.065 120.036 119.914 0.095 0.000 2.876 39 V HA 0.777 4.898 4.120 0.002 0.000 0.312 39 V C -0.667 175.514 176.094 0.145 0.000 1.085 39 V CA -0.539 61.841 62.300 0.133 0.000 0.945 39 V CB 1.529 33.413 31.823 0.102 0.000 1.017 39 V HN 1.090 nan 8.190 nan 0.000 0.428 40 H N 1.688 120.751 119.070 -0.011 0.000 3.017 40 H HA 0.766 5.322 4.556 0.000 0.000 0.340 40 H C -2.145 173.157 175.328 -0.043 0.000 1.014 40 H CA -0.612 55.422 56.048 -0.023 0.000 1.341 40 H CB 2.330 32.071 29.762 -0.036 0.000 1.739 40 H HN 0.713 nan 8.280 nan 0.000 0.506 41 V N 6.077 126.093 119.914 0.170 0.000 2.487 41 V HA 0.267 4.388 4.120 0.002 0.000 0.298 41 V C -0.458 175.649 176.094 0.021 0.000 1.028 41 V CA -0.730 61.605 62.300 0.058 0.000 0.860 41 V CB 1.691 33.641 31.823 0.213 0.000 0.991 41 V HN 0.534 nan 8.190 nan 0.000 0.427 42 V N 7.328 127.187 119.914 -0.092 0.000 2.305 42 V HA 0.399 4.520 4.120 0.002 0.000 0.275 42 V C -2.113 173.964 176.094 -0.029 0.000 1.020 42 V CA -1.309 60.950 62.300 -0.068 0.000 0.811 42 V CB 1.403 33.127 31.823 -0.165 0.000 1.031 42 V HN 0.713 nan 8.190 nan 0.000 0.439 43 P HA 0.358 nan 4.420 nan 0.000 0.297 43 P C -0.268 177.031 177.300 -0.002 0.000 1.307 43 P CA -0.100 63.000 63.100 -0.001 0.000 0.773 43 P CB 0.844 32.547 31.700 0.005 0.000 1.265 44 D N -2.580 117.817 120.400 -0.004 0.000 2.837 44 D HA -0.118 4.524 4.640 0.002 0.000 0.230 44 D C -0.088 176.212 176.300 -0.001 0.000 1.152 44 D CA 1.020 55.019 54.000 -0.002 0.000 0.736 44 D CB -0.762 40.039 40.800 0.002 0.000 1.084 44 D HN 0.396 nan 8.370 nan 0.000 0.429 45 Q N -0.243 119.556 119.800 -0.002 0.000 2.306 45 Q HA 0.406 4.747 4.340 0.002 0.000 0.241 45 Q C 0.855 176.858 176.000 0.005 0.000 0.948 45 Q CA -0.291 55.512 55.803 -0.000 0.000 0.886 45 Q CB 0.987 29.723 28.738 -0.004 0.000 1.227 45 Q HN 0.340 nan 8.270 nan 0.000 0.457 46 L N 4.202 125.428 121.223 0.006 0.000 2.334 46 L HA 0.336 4.677 4.340 0.002 0.000 0.286 46 L C 0.155 177.033 176.870 0.014 0.000 1.108 46 L CA -0.052 54.793 54.840 0.009 0.000 0.875 46 L CB -0.437 41.627 42.059 0.008 0.000 1.246 46 L HN 0.475 nan 8.230 nan 0.000 0.439 47 M N 2.020 121.632 119.600 0.020 0.000 2.664 47 M HA 0.974 5.455 4.480 0.002 0.000 0.279 47 M C -1.284 175.041 176.300 0.042 0.000 1.275 47 M CA -0.735 54.583 55.300 0.031 0.000 0.829 47 M CB 2.573 35.197 32.600 0.040 0.000 1.727 47 M HN 0.225 nan 8.290 nan 0.000 0.459 48 A N 0.998 123.849 122.820 0.052 0.000 2.515 48 A HA 0.883 5.204 4.320 0.002 0.000 0.298 48 A C -2.275 175.371 177.584 0.103 0.000 1.059 48 A CA -0.564 51.514 52.037 0.069 0.000 0.698 48 A CB 1.762 20.786 19.000 0.040 0.000 1.289 48 A HN 0.888 nan 8.150 nan 0.000 0.404 49 F N 1.422 121.351 119.950 -0.035 0.000 2.539 49 F HA 0.530 5.058 4.527 0.002 0.000 0.328 49 F C 1.040 176.811 175.800 -0.049 0.000 1.148 49 F CA 0.767 58.729 58.000 -0.063 0.000 0.940 49 F CB 1.855 40.780 39.000 -0.124 0.000 1.194 49 F HN 1.472 nan 8.300 nan 0.000 0.438 50 G N 3.273 112.088 108.800 0.026 0.000 2.179 50 G HA2 -0.049 3.912 3.960 0.002 0.000 0.257 50 G HA3 -0.049 3.912 3.960 0.002 0.000 0.257 50 G C 1.066 176.004 174.900 0.064 0.000 1.010 50 G CA 0.512 45.662 45.100 0.084 0.000 0.736 50 G HN 2.146 nan 8.290 nan 0.000 0.513 51 G N -1.863 106.961 108.800 0.040 0.000 2.179 51 G HA2 0.024 3.985 3.960 0.002 0.000 0.260 51 G HA3 0.024 3.985 3.960 0.002 0.000 0.260 51 G C 0.618 175.543 174.900 0.042 0.000 0.977 51 G CA 1.479 46.598 45.100 0.032 0.000 0.641 51 G HN 2.280 nan 8.290 nan 0.000 0.533 52 S N -0.036 115.703 115.700 0.066 0.000 2.525 52 S HA 0.645 5.116 4.470 0.002 0.000 0.290 52 S C 1.290 175.922 174.600 0.053 0.000 1.152 52 S CA 0.746 58.977 58.200 0.052 0.000 1.072 52 S CB 1.524 64.754 63.200 0.049 0.000 1.027 52 S HN 1.289 nan 8.310 nan 0.000 0.500 53 S N 2.561 118.279 115.700 0.030 0.000 2.614 53 S HA 0.262 4.734 4.470 0.002 0.000 0.230 53 S C 0.208 174.814 174.600 0.010 0.000 0.952 53 S CA -0.556 57.658 58.200 0.024 0.000 0.949 53 S CB -0.213 62.996 63.200 0.016 0.000 0.786 53 S HN 0.711 nan 8.310 nan 0.000 0.478 54 E N 2.840 123.041 120.200 0.003 0.000 2.409 54 E HA 0.255 4.606 4.350 0.002 0.000 0.257 54 E C -2.569 174.013 176.600 -0.029 0.000 1.150 54 E CA -2.235 54.154 56.400 -0.018 0.000 0.942 54 E CB -0.323 29.360 29.700 -0.030 0.000 0.979 54 E HN 0.153 nan 8.360 nan 0.000 0.447 55 P HA -0.097 nan 4.420 nan 0.000 0.261 55 P C -0.523 176.728 177.300 -0.081 0.000 1.173 55 P CA 0.470 63.539 63.100 -0.052 0.000 0.760 55 P CB 0.239 31.905 31.700 -0.058 0.000 0.783 56 C N 1.501 120.765 119.300 -0.059 0.000 3.332 56 C HA 0.953 5.414 4.460 0.002 0.000 0.329 56 C C -1.193 173.785 174.990 -0.019 0.000 1.434 56 C CA -0.997 57.970 59.018 -0.085 0.000 1.314 56 C CB 1.220 28.947 27.740 -0.022 0.000 1.664 56 C HN 0.657 nan 8.230 nan 0.000 0.457 57 A N 0.793 123.615 122.820 0.004 0.000 2.547 57 A HA 0.805 5.126 4.320 0.002 0.000 0.297 57 A C -1.596 176.053 177.584 0.107 0.000 1.056 57 A CA -0.438 51.627 52.037 0.045 0.000 0.688 57 A CB 0.999 20.022 19.000 0.039 0.000 1.282 57 A HN 1.107 nan 8.150 nan 0.000 0.400 58 L N 1.688 122.966 121.223 0.091 0.000 2.319 58 L HA 0.575 4.916 4.340 0.002 0.000 0.281 58 L C -0.703 176.185 176.870 0.029 0.000 1.005 58 L CA -0.502 54.410 54.840 0.121 0.000 0.828 58 L CB 1.385 43.511 42.059 0.111 0.000 1.227 58 L HN 0.814 nan 8.230 nan 0.000 0.415 59 C N 0.949 120.267 119.300 0.031 0.000 2.973 59 C HA 0.898 5.359 4.460 0.002 0.000 0.329 59 C C 0.091 175.049 174.990 -0.054 0.000 1.327 59 C CA -0.802 58.165 59.018 -0.086 0.000 1.632 59 C CB 2.012 29.712 27.740 -0.065 0.000 2.098 59 C HN 0.844 nan 8.230 nan 0.000 0.469 60 S N 0.278 115.925 115.700 -0.088 0.000 2.536 60 S HA 0.771 5.243 4.470 0.002 0.000 0.271 60 S C -1.589 173.084 174.600 0.121 0.000 1.134 60 S CA -0.526 57.689 58.200 0.026 0.000 0.897 60 S CB 1.502 64.823 63.200 0.201 0.000 1.094 60 S HN 0.887 nan 8.310 nan 0.000 0.473 61 L N 2.471 123.731 121.223 0.061 0.000 2.404 61 L HA 0.668 5.009 4.340 0.002 0.000 0.272 61 L C -1.688 175.271 176.870 0.149 0.000 0.980 61 L CA -0.128 54.831 54.840 0.198 0.000 0.836 61 L CB 1.201 43.335 42.059 0.124 0.000 1.238 61 L HN 0.965 nan 8.230 nan 0.000 0.408 62 H N 2.520 121.714 119.070 0.207 0.000 2.457 62 H HA 0.837 5.393 4.556 -0.000 0.000 0.335 62 H C -0.666 174.726 175.328 0.107 0.000 1.115 62 H CA -0.308 55.859 56.048 0.198 0.000 1.219 62 H CB 1.897 31.732 29.762 0.121 0.000 1.471 62 H HN 0.595 nan 8.280 nan 0.000 0.491 63 S N 2.129 117.946 115.700 0.196 0.000 2.537 63 S HA 0.415 4.886 4.470 0.002 0.000 0.270 63 S C -1.081 173.551 174.600 0.054 0.000 1.142 63 S CA -0.789 57.459 58.200 0.079 0.000 0.870 63 S CB 0.749 63.970 63.200 0.035 0.000 1.112 63 S HN 0.558 nan 8.310 nan 0.000 0.466 64 I N 4.376 124.928 120.570 -0.029 0.000 2.278 64 I HA 0.487 4.658 4.170 0.002 0.000 0.296 64 I C 1.065 177.156 176.117 -0.044 0.000 1.121 64 I CA 0.545 61.820 61.300 -0.041 0.000 1.267 64 I CB -0.187 37.735 38.000 -0.130 0.000 1.447 64 I HN 1.020 nan 8.210 nan 0.000 0.509 65 G N 5.667 114.468 108.800 0.001 0.000 2.782 65 G HA2 -0.220 3.741 3.960 0.002 0.000 0.228 65 G HA3 -0.220 3.741 3.960 0.002 0.000 0.228 65 G C 0.258 175.171 174.900 0.021 0.000 1.372 65 G CA -0.671 44.429 45.100 0.000 0.000 0.862 65 G HN 0.593 nan 8.290 nan 0.000 0.547 66 K N -2.132 118.283 120.400 0.025 0.000 3.230 66 K HA -0.163 4.158 4.320 0.002 0.000 0.285 66 K C 0.319 177.008 176.600 0.147 0.000 1.196 66 K CA 1.661 57.991 56.287 0.071 0.000 0.838 66 K CB -1.552 31.004 32.500 0.094 0.000 1.262 66 K HN 0.908 nan 8.250 nan 0.000 0.492 67 I N 0.267 120.887 120.570 0.082 0.000 2.436 67 I HA 0.671 4.842 4.170 0.002 0.000 0.289 67 I C 0.874 176.982 176.117 -0.014 0.000 1.010 67 I CA -0.172 61.174 61.300 0.076 0.000 1.098 67 I CB 1.810 39.863 38.000 0.088 0.000 1.266 67 I HN 0.252 nan 8.210 nan 0.000 0.434 68 G N 2.955 111.704 108.800 -0.084 0.000 2.349 68 G HA2 0.419 4.381 3.960 0.002 0.000 0.294 68 G HA3 0.419 4.381 3.960 0.002 0.000 0.294 68 G C 0.415 175.223 174.900 -0.153 0.000 1.380 68 G CA -0.080 44.960 45.100 -0.100 0.000 0.811 68 G HN 0.699 nan 8.290 nan 0.000 0.519 69 G N 0.220 108.950 108.800 -0.118 0.000 2.586 69 G HA2 0.085 4.047 3.960 0.002 0.000 0.218 69 G HA3 0.085 4.047 3.960 0.002 0.000 0.218 69 G C 2.070 176.877 174.900 -0.156 0.000 1.216 69 G CA 2.867 47.895 45.100 -0.120 0.000 0.786 69 G HN 1.677 nan 8.290 nan 0.000 0.583 70 A N 0.022 122.751 122.820 -0.151 0.000 1.898 70 A HA -0.049 4.272 4.320 0.002 0.000 0.216 70 A C 2.389 179.813 177.584 -0.267 0.000 1.181 70 A CA 1.992 53.927 52.037 -0.170 0.000 0.620 70 A CB -0.458 18.460 19.000 -0.137 0.000 0.819 70 A HN 0.512 nan 8.150 nan 0.000 0.442 71 Q N -0.281 119.320 119.800 -0.332 0.000 2.084 71 Q HA -0.179 4.162 4.340 0.002 0.000 0.202 71 Q C 1.779 177.283 176.000 -0.825 0.000 0.978 71 Q CA 1.484 56.930 55.803 -0.596 0.000 0.844 71 Q CB -0.282 28.152 28.738 -0.507 0.000 0.898 71 Q HN 0.622 nan 8.270 nan 0.000 0.426 72 N N 0.619 118.985 118.700 -0.556 0.000 2.223 72 N HA -0.108 4.633 4.740 0.002 0.000 0.185 72 N C 1.536 176.823 175.510 -0.373 0.000 1.016 72 N CA 1.009 53.672 53.050 -0.645 0.000 0.863 72 N CB -0.157 37.886 38.487 -0.740 0.000 0.983 72 N HN 0.204 nan 8.380 nan 0.000 0.429 73 R N 0.018 120.364 120.500 -0.257 0.000 2.075 73 R HA 0.006 4.347 4.340 0.002 0.000 0.232 73 R C 2.247 178.480 176.300 -0.111 0.000 1.126 73 R CA 1.128 57.153 56.100 -0.125 0.000 0.963 73 R CB -0.372 29.866 30.300 -0.103 0.000 0.858 73 R HN 0.150 nan 8.270 nan 0.000 0.435 74 S N -0.030 115.542 115.700 -0.213 0.000 2.368 74 S HA -0.129 4.342 4.470 0.002 0.000 0.224 74 S C 1.820 176.375 174.600 -0.074 0.000 1.029 74 S CA 1.011 59.103 58.200 -0.181 0.000 0.988 74 S CB -0.211 62.818 63.200 -0.284 0.000 0.838 74 S HN 0.384 nan 8.310 nan 0.000 0.462 75 Y N 1.364 121.624 120.300 -0.066 0.000 2.181 75 Y HA -0.124 4.417 4.550 -0.016 0.000 0.288 75 Y C 3.073 179.019 175.900 0.078 0.000 1.146 75 Y CA 0.962 59.060 58.100 -0.003 0.000 1.164 75 Y CB -0.364 38.116 38.460 0.033 0.000 0.982 75 Y HN 0.281 nan 8.280 nan 0.000 0.515 76 S N 0.012 115.883 115.700 0.285 0.000 2.368 76 S HA -0.213 4.258 4.470 0.002 0.000 0.225 76 S C 1.908 176.593 174.600 0.142 0.000 1.030 76 S CA 1.380 59.734 58.200 0.256 0.000 0.999 76 S CB -0.253 63.088 63.200 0.236 0.000 0.844 76 S HN 0.346 nan 8.310 nan 0.000 0.459 77 K N 0.777 121.230 120.400 0.087 0.000 2.026 77 K HA -0.095 4.226 4.320 0.002 0.000 0.208 77 K C 2.186 178.819 176.600 0.055 0.000 1.048 77 K CA 1.143 57.462 56.287 0.054 0.000 0.929 77 K CB -0.290 32.222 32.500 0.021 0.000 0.713 77 K HN 0.231 nan 8.250 nan 0.000 0.439 78 L N 1.550 122.809 121.223 0.060 0.000 1.994 78 L HA -0.160 4.181 4.340 0.002 0.000 0.208 78 L C 1.998 178.890 176.870 0.037 0.000 1.071 78 L CA 1.639 56.506 54.840 0.044 0.000 0.745 78 L CB -0.425 41.664 42.059 0.050 0.000 0.892 78 L HN 0.223 nan 8.230 nan 0.000 0.431 79 L N -1.611 119.635 121.223 0.038 0.000 2.056 79 L HA -0.229 4.112 4.340 0.002 0.000 0.207 79 L C 2.612 179.547 176.870 0.108 0.000 1.078 79 L CA 1.211 56.066 54.840 0.026 0.000 0.749 79 L CB -0.811 41.235 42.059 -0.021 0.000 0.901 79 L HN 0.376 nan 8.230 nan 0.000 0.433 80 C N 0.154 119.518 119.300 0.106 0.000 2.425 80 C HA -0.082 4.379 4.460 0.002 0.000 0.277 80 C C 2.922 177.955 174.990 0.072 0.000 1.280 80 C CA 0.845 59.918 59.018 0.091 0.000 1.744 80 C CB -1.488 26.296 27.740 0.074 0.000 1.989 80 C HN 0.707 nan 8.230 nan 0.000 0.491 81 G N 0.319 109.157 108.800 0.064 0.000 2.418 81 G HA2 -0.176 3.785 3.960 0.002 0.000 0.217 81 G HA3 -0.176 3.785 3.960 0.002 0.000 0.217 81 G C 1.563 176.507 174.900 0.073 0.000 1.158 81 G CA 0.642 45.774 45.100 0.053 0.000 0.771 81 G HN 0.517 nan 8.290 nan 0.000 0.545 82 L N -0.135 121.149 121.223 0.102 0.000 2.056 82 L HA 0.037 4.378 4.340 0.002 0.000 0.207 82 L C 2.889 179.885 176.870 0.209 0.000 1.078 82 L CA 0.505 55.446 54.840 0.168 0.000 0.749 82 L CB -0.316 41.855 42.059 0.187 0.000 0.901 82 L HN 0.179 nan 8.230 nan 0.000 0.433 83 L N -0.698 120.641 121.223 0.193 0.000 2.093 83 L HA -0.174 4.168 4.340 0.002 0.000 0.208 83 L C 2.816 179.711 176.870 0.043 0.000 1.085 83 L CA 1.039 55.967 54.840 0.147 0.000 0.755 83 L CB -0.697 41.450 42.059 0.147 0.000 0.904 83 L HN 0.242 nan 8.230 nan 0.000 0.435 84 A N -0.249 122.594 122.820 0.039 0.000 1.872 84 A HA -0.226 4.095 4.320 0.002 0.000 0.214 84 A C 2.286 179.869 177.584 -0.001 0.000 1.187 84 A CA 1.647 53.687 52.037 0.004 0.000 0.614 84 A CB -0.427 18.576 19.000 0.005 0.000 0.826 84 A HN 0.430 nan 8.150 nan 0.000 0.442 85 E N -0.832 119.380 120.200 0.020 0.000 2.072 85 E HA -0.180 4.171 4.350 0.002 0.000 0.190 85 E C 2.139 178.736 176.600 -0.006 0.000 0.982 85 E CA 0.850 57.259 56.400 0.014 0.000 0.803 85 E CB 0.022 29.744 29.700 0.037 0.000 0.755 85 E HN 0.339 nan 8.360 nan 0.000 0.453 86 R N -0.179 120.313 120.500 -0.013 0.000 2.112 86 R HA 0.106 4.447 4.340 0.002 0.000 0.216 86 R C 2.096 178.265 176.300 -0.217 0.000 1.080 86 R CA 0.584 56.618 56.100 -0.110 0.000 0.996 86 R CB -0.144 30.073 30.300 -0.139 0.000 0.902 86 R HN 0.284 nan 8.270 nan 0.000 0.449 87 L N 0.531 121.635 121.223 -0.199 0.000 2.693 87 L HA 0.280 4.621 4.340 0.002 0.000 0.235 87 L C -0.074 176.734 176.870 -0.104 0.000 1.127 87 L CA -0.302 54.424 54.840 -0.190 0.000 0.914 87 L CB 0.274 42.210 42.059 -0.205 0.000 1.193 87 L HN -0.042 nan 8.230 nan 0.000 0.502 88 R N 0.855 121.310 120.500 -0.075 0.000 3.531 88 R HA -0.125 4.216 4.340 0.002 0.000 0.280 88 R C -0.441 175.825 176.300 -0.056 0.000 1.130 88 R CA 0.670 56.736 56.100 -0.055 0.000 0.757 88 R CB -2.691 27.577 30.300 -0.053 0.000 1.218 88 R HN 0.347 nan 8.270 nan 0.000 0.454 89 I N 0.473 121.011 120.570 -0.055 0.000 2.336 89 I HA 0.123 4.295 4.170 0.002 0.000 0.292 89 I C 1.147 177.221 176.117 -0.073 0.000 0.991 89 I CA -0.454 60.806 61.300 -0.067 0.000 1.227 89 I CB 1.766 39.731 38.000 -0.059 0.000 1.366 89 I HN 0.012 nan 8.210 nan 0.000 0.466 90 S N 7.228 122.867 115.700 -0.101 0.000 2.537 90 S HA 0.140 4.611 4.470 0.002 0.000 0.286 90 S C -1.446 173.076 174.600 -0.129 0.000 1.299 90 S CA -0.959 57.177 58.200 -0.106 0.000 1.067 90 S CB 0.829 63.954 63.200 -0.124 0.000 0.864 90 S HN 0.375 nan 8.310 nan 0.000 0.494 91 P HA -0.135 nan 4.420 nan 0.000 0.217 91 P C 0.613 177.841 177.300 -0.119 0.000 1.148 91 P CA 1.193 64.254 63.100 -0.065 0.000 0.828 91 P CB -0.042 31.645 31.700 -0.021 0.000 0.783 92 D N -1.656 118.650 120.400 -0.157 0.000 2.352 92 D HA -0.081 4.560 4.640 0.002 0.000 0.232 92 D C 0.899 176.886 176.300 -0.521 0.000 1.055 92 D CA 0.410 54.288 54.000 -0.205 0.000 0.891 92 D CB -0.575 40.154 40.800 -0.119 0.000 0.897 92 D HN 0.167 nan 8.370 nan 0.000 0.529 93 R N -0.092 120.007 120.500 -0.669 0.000 2.690 93 R HA 0.333 4.674 4.340 0.002 0.000 0.419 93 R C -0.993 174.738 176.300 -0.947 0.000 1.090 93 R CA -0.254 55.052 56.100 -1.323 0.000 1.064 93 R CB 1.485 31.316 30.300 -0.781 0.000 1.391 93 R HN -0.006 nan 8.270 nan 0.000 0.586 94 V N 0.831 120.423 119.914 -0.537 0.000 2.588 94 V HA 0.405 4.526 4.120 0.002 0.000 0.304 94 V C -0.860 175.298 176.094 0.106 0.000 1.042 94 V CA -0.765 61.462 62.300 -0.122 0.000 0.877 94 V CB 2.181 33.997 31.823 -0.012 0.000 0.996 94 V HN 0.195 nan 8.190 nan 0.000 0.425 95 Y N 4.391 124.827 120.300 0.226 0.000 2.409 95 Y HA 0.682 5.233 4.550 0.001 0.000 0.343 95 Y C -0.182 175.760 175.900 0.069 0.000 0.973 95 Y CA -1.025 57.182 58.100 0.179 0.000 1.064 95 Y CB 2.374 40.943 38.460 0.182 0.000 1.207 95 Y HN 0.457 nan 8.280 nan 0.000 0.452 96 I N 3.682 124.352 120.570 0.167 0.000 2.468 96 I HA 0.228 4.400 4.170 0.002 0.000 0.285 96 I C -1.080 174.842 176.117 -0.325 0.000 1.039 96 I CA -0.480 60.765 61.300 -0.091 0.000 1.074 96 I CB 1.596 39.497 38.000 -0.165 0.000 1.228 96 I HN 0.607 nan 8.210 nan 0.000 0.436 97 N N 5.503 124.017 118.700 -0.311 0.000 2.434 97 N HA 0.398 5.140 4.740 0.002 0.000 0.272 97 N C -1.425 173.675 175.510 -0.684 0.000 1.040 97 N CA -0.437 52.358 53.050 -0.425 0.000 0.956 97 N CB 0.926 39.252 38.487 -0.270 0.000 1.108 97 N HN 0.380 nan 8.380 nan 0.000 0.481 98 Y N 1.837 121.864 120.300 -0.456 0.000 2.342 98 Y HA 0.352 4.901 4.550 -0.002 0.000 0.334 98 Y C -0.714 174.802 175.900 -0.640 0.000 1.067 98 Y CA -0.579 57.304 58.100 -0.361 0.000 1.128 98 Y CB 0.862 39.248 38.460 -0.123 0.000 1.200 98 Y HN 0.428 nan 8.280 nan 0.000 0.464 99 Y N 1.262 121.543 120.300 -0.032 0.000 2.338 99 Y HA 0.223 4.773 4.550 -0.001 0.000 0.333 99 Y C -0.589 175.333 175.900 0.037 0.000 0.968 99 Y CA -1.419 56.650 58.100 -0.052 0.000 1.123 99 Y CB 1.439 39.770 38.460 -0.216 0.000 1.165 99 Y HN 0.500 nan 8.280 nan 0.000 0.452 100 D N 4.462 124.965 120.400 0.171 0.000 2.411 100 D HA 0.202 4.843 4.640 0.002 0.000 0.225 100 D C -0.533 175.852 176.300 0.142 0.000 1.156 100 D CA -0.179 53.899 54.000 0.129 0.000 0.874 100 D CB 0.529 41.377 40.800 0.079 0.000 1.034 100 D HN 0.335 nan 8.370 nan 0.000 0.502 101 M N 2.493 122.183 119.600 0.150 0.000 2.235 101 M HA 0.170 4.651 4.480 0.002 0.000 0.351 101 M C 0.611 176.967 176.300 0.094 0.000 1.178 101 M CA -0.413 54.970 55.300 0.139 0.000 1.143 101 M CB 0.705 33.399 32.600 0.157 0.000 1.530 101 M HN 0.327 nan 8.290 nan 0.000 0.461 102 N N 1.234 119.988 118.700 0.091 0.000 2.514 102 N HA 0.212 4.954 4.740 0.002 0.000 0.277 102 N C 0.611 176.177 175.510 0.094 0.000 1.126 102 N CA 0.166 53.261 53.050 0.076 0.000 0.978 102 N CB 1.444 39.972 38.487 0.069 0.000 1.106 102 N HN 0.718 nan 8.380 nan 0.000 0.461 103 A N 3.931 126.805 122.820 0.090 0.000 1.986 103 A HA -0.159 4.163 4.320 0.002 0.000 0.220 103 A C 2.049 179.740 177.584 0.178 0.000 1.171 103 A CA 2.048 54.172 52.037 0.146 0.000 0.640 103 A CB -0.803 18.274 19.000 0.129 0.000 0.811 103 A HN 0.823 nan 8.150 nan 0.000 0.451 104 A N -0.480 122.412 122.820 0.120 0.000 2.121 104 A HA -0.074 4.247 4.320 0.002 0.000 0.218 104 A C 1.426 179.068 177.584 0.096 0.000 1.154 104 A CA 1.297 53.394 52.037 0.101 0.000 0.679 104 A CB -0.329 18.714 19.000 0.072 0.000 0.795 104 A HN 0.500 nan 8.150 nan 0.000 0.458 105 N N -0.278 118.485 118.700 0.104 0.000 2.251 105 N HA 0.195 4.936 4.740 0.002 0.000 0.217 105 N C -0.870 174.708 175.510 0.114 0.000 1.124 105 N CA 0.300 53.405 53.050 0.092 0.000 0.843 105 N CB 1.018 39.553 38.487 0.080 0.000 1.024 105 N HN 0.144 nan 8.380 nan 0.000 0.501 106 V N 0.228 120.243 119.914 0.169 0.000 2.409 106 V HA 0.562 4.683 4.120 0.002 0.000 0.290 106 V C 0.568 176.787 176.094 0.208 0.000 1.017 106 V CA -1.115 61.322 62.300 0.228 0.000 0.841 106 V CB 1.468 33.517 31.823 0.376 0.000 1.003 106 V HN 0.112 nan 8.190 nan 0.000 0.426 107 G N 3.116 111.993 108.800 0.128 0.000 2.420 107 G HA2 0.568 4.529 3.960 0.002 0.000 0.284 107 G HA3 0.568 4.529 3.960 0.002 0.000 0.284 107 G C -1.467 173.499 174.900 0.111 0.000 1.177 107 G CA -0.386 44.751 45.100 0.061 0.000 0.841 107 G HN 0.886 nan 8.290 nan 0.000 0.527 108 W N 1.823 122.973 121.300 -0.252 0.000 3.901 108 W HA 0.368 5.037 4.660 0.015 0.000 0.274 108 W C -0.171 176.194 176.519 -0.256 0.000 1.278 108 W CA -0.830 56.353 57.345 -0.270 0.000 1.235 108 W CB 0.365 29.531 29.460 -0.489 0.000 1.261 108 W HN 0.836 nan 8.180 nan 0.000 0.546 109 N N 4.736 122.747 118.700 -1.149 0.000 2.714 109 N HA -0.332 4.409 4.740 0.002 0.000 0.253 109 N C 0.317 175.483 175.510 -0.573 0.000 1.024 109 N CA 1.548 53.929 53.050 -1.114 0.000 0.726 109 N CB -0.957 36.317 38.487 -2.021 0.000 0.908 109 N HN 0.865 nan 8.380 nan 0.000 0.542 110 N N -1.888 116.598 118.700 -0.357 0.000 2.863 110 N HA -0.237 4.505 4.740 0.002 0.000 0.245 110 N C -0.437 174.975 175.510 -0.165 0.000 1.001 110 N CA 1.182 54.101 53.050 -0.217 0.000 0.901 110 N CB -0.749 37.621 38.487 -0.194 0.000 1.124 110 N HN 0.506 nan 8.380 nan 0.000 0.582 111 S N -2.094 113.500 115.700 -0.176 0.000 3.287 111 S HA 0.732 5.203 4.470 0.002 0.000 0.324 111 S C -0.705 173.844 174.600 -0.085 0.000 1.205 111 S CA 0.139 58.278 58.200 -0.103 0.000 1.020 111 S CB 1.184 64.334 63.200 -0.084 0.000 1.398 111 S HN 0.279 nan 8.310 nan 0.000 0.679 112 T N -1.216 113.310 114.554 -0.047 0.000 2.858 112 T HA 0.625 4.976 4.350 0.002 0.000 0.285 112 T C -0.047 174.636 174.700 -0.028 0.000 1.052 112 T CA -0.476 61.609 62.100 -0.025 0.000 1.009 112 T CB 0.529 69.429 68.868 0.054 0.000 1.241 112 T HN 0.379 nan 8.240 nan 0.000 0.542 113 F N 0.892 120.933 119.950 0.152 0.000 2.797 113 F HA 0.477 5.010 4.527 0.010 0.000 0.302 113 F C 1.954 177.801 175.800 0.077 0.000 1.130 113 F CA -0.258 57.815 58.000 0.122 0.000 1.387 113 F CB -0.257 38.780 39.000 0.063 0.000 1.107 113 F HN 0.784 nan 8.300 nan 0.000 0.577 114 A N 0.000 122.945 122.820 0.209 0.000 2.254 114 A HA 0.000 4.321 4.320 0.002 0.000 0.244 114 A CA 0.000 52.119 52.037 0.137 0.000 0.836 114 A CB 0.000 19.058 19.000 0.096 0.000 0.831 114 A HN 0.000 nan 8.150 nan 0.000 0.486