REMARK SPARTA+ Protein Chemical Shift Prediction Table REMARK REMARK All chemical shifts are reported in ppm: REMARK REMARK SS_SHIFT is the predicted secondary chemical shift. REMARK SHIFT is the predicted chemical shift. REMARK RC_SHIFT is the random coil chemical shift. REMARK HM_SHIFT is the ring current shift; a correction REMARK of 0.6 times this value is applied. REMARK EF_SHIFT is the adjustment for electric field effect, REMARK applied to proton shifts only. REMARK SIGMA is the estimated prediction error. REMARK REMARK Reference: REMARK Y. Shen and A. Bax: SPARTA+: a modest improvement in empirical NMR REMARK chemical shift prediction by means of an artificial neural network. REMARK J. Biomol. NMR 48, 13-22 (2010). DATA SOURCE 2ooz_1_B DATA FIRST_RESID 1 DATA SEQUENCE PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG DATA SEQUENCE GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD DATA SEQUENCE MNAANVGWNN STFA VARS RESID RESNAME ATOMNAME SS_SHIFT SHIFT RC_SHIFT HM_SHIFT EF_SHIFT SIGMA FORMAT %4d %4s %4s %9.3f %9.3f %9.3f %9.3f %9.3f %9.3f 1 P HA 0.000 nan 4.420 nan 0.000 0.216 1 P C 0.000 177.345 177.300 0.075 0.000 1.155 1 P CA 0.000 63.111 63.100 0.018 0.000 0.800 1 P CB 0.000 31.683 31.700 -0.028 0.000 0.726 2 M N 0.617 120.302 119.600 0.141 0.000 2.327 2 M HA 0.777 5.257 4.480 0.000 0.000 0.298 2 M C -1.666 174.794 176.300 0.266 0.000 1.065 2 M CA -0.658 54.742 55.300 0.167 0.000 0.916 2 M CB 2.709 35.375 32.600 0.110 0.000 1.630 2 M HN 0.246 nan 8.290 nan 0.000 0.442 3 F N 4.343 124.336 119.950 0.071 0.000 2.507 3 F HA 0.816 5.343 4.527 0.000 0.000 0.325 3 F C -1.817 174.030 175.800 0.078 0.000 1.116 3 F CA -1.089 56.947 58.000 0.060 0.000 0.930 3 F CB 1.336 40.333 39.000 -0.006 0.000 1.146 3 F HN 0.651 nan 8.300 nan 0.000 0.447 4 I N 6.895 127.035 120.570 -0.716 0.000 2.498 4 I HA 0.513 4.683 4.170 0.000 0.000 0.290 4 I C -1.309 174.299 176.117 -0.848 0.000 1.032 4 I CA -1.238 59.715 61.300 -0.578 0.000 1.073 4 I CB 2.039 39.889 38.000 -0.250 0.000 1.251 4 I HN 0.429 nan 8.210 nan 0.000 0.426 5 V N 7.002 126.574 119.914 -0.571 0.000 2.483 5 V HA 0.502 4.623 4.120 0.000 0.000 0.297 5 V C -1.023 174.960 176.094 -0.184 0.000 1.027 5 V CA -0.303 61.801 62.300 -0.327 0.000 0.855 5 V CB 1.807 33.582 31.823 -0.082 0.000 0.995 5 V HN 0.721 nan 8.190 nan 0.000 0.424 6 N N 4.063 122.687 118.700 -0.127 0.000 2.408 6 N HA 0.534 5.274 4.740 0.000 0.000 0.280 6 N C -0.801 174.692 175.510 -0.027 0.000 1.002 6 N CA -0.160 52.839 53.050 -0.085 0.000 0.907 6 N CB 2.072 40.516 38.487 -0.072 0.000 1.161 6 N HN 0.772 nan 8.380 nan 0.000 0.488 7 T N 0.123 114.666 114.554 -0.019 0.000 2.894 7 T HA 0.185 4.535 4.350 0.000 0.000 0.309 7 T C 0.364 175.059 174.700 -0.009 0.000 1.208 7 T CA -0.701 61.395 62.100 -0.006 0.000 1.016 7 T CB 0.743 69.608 68.868 -0.006 0.000 1.192 7 T HN 0.559 nan 8.240 nan 0.000 0.491 8 N N 2.076 120.772 118.700 -0.007 0.000 2.398 8 N HA 0.049 4.789 4.740 0.000 0.000 0.188 8 N C 0.502 176.001 175.510 -0.017 0.000 1.122 8 N CA 0.035 53.081 53.050 -0.007 0.000 0.866 8 N CB -0.370 38.116 38.487 -0.001 0.000 0.970 8 N HN 0.317 nan 8.380 nan 0.000 0.462 9 V N 3.343 123.241 119.914 -0.028 0.000 2.763 9 V HA 0.117 4.237 4.120 0.000 0.000 0.306 9 V C -1.762 174.311 176.094 -0.034 0.000 1.059 9 V CA -1.051 61.225 62.300 -0.040 0.000 1.138 9 V CB 0.758 32.544 31.823 -0.062 0.000 0.940 9 V HN 0.234 nan 8.190 nan 0.000 0.489 10 P HA 0.131 nan 4.420 nan 0.000 0.274 10 P C 0.462 177.744 177.300 -0.029 0.000 1.231 10 P CA -0.418 62.667 63.100 -0.025 0.000 0.790 10 P CB 0.871 32.558 31.700 -0.022 0.000 0.951 11 R N 2.870 123.360 120.500 -0.015 0.000 2.103 11 R HA -0.201 4.139 4.340 0.000 0.000 0.242 11 R C 2.032 178.321 176.300 -0.017 0.000 1.142 11 R CA 2.222 58.317 56.100 -0.009 0.000 0.960 11 R CB -1.018 29.285 30.300 0.006 0.000 0.858 11 R HN 0.546 nan 8.270 nan 0.000 0.439 12 A N -0.495 122.315 122.820 -0.018 0.000 2.125 12 A HA -0.060 4.260 4.320 0.000 0.000 0.219 12 A C 1.931 179.494 177.584 -0.035 0.000 1.156 12 A CA 1.627 53.652 52.037 -0.019 0.000 0.671 12 A CB -0.171 18.820 19.000 -0.015 0.000 0.794 12 A HN 0.406 nan 8.150 nan 0.000 0.459 13 S N -0.772 114.897 115.700 -0.051 0.000 2.558 13 S HA 0.136 4.606 4.470 0.000 0.000 0.217 13 S C 0.423 174.948 174.600 -0.125 0.000 0.975 13 S CA -0.047 58.108 58.200 -0.076 0.000 0.912 13 S CB 0.051 63.205 63.200 -0.076 0.000 0.776 13 S HN 0.231 nan 8.310 nan 0.000 0.526 14 V N 5.556 125.396 119.914 -0.123 0.000 2.389 14 V HA 0.223 4.343 4.120 0.000 0.000 0.264 14 V C -1.896 174.131 176.094 -0.111 0.000 1.049 14 V CA -1.973 60.210 62.300 -0.196 0.000 0.932 14 V CB 0.208 31.964 31.823 -0.112 0.000 1.011 14 V HN 0.226 nan 8.190 nan 0.000 0.475 15 P HA 0.154 nan 4.420 nan 0.000 0.271 15 P C -0.474 176.863 177.300 0.061 0.000 1.218 15 P CA -0.455 62.630 63.100 -0.025 0.000 0.780 15 P CB 0.786 32.470 31.700 -0.026 0.000 0.901 16 D N 1.046 121.479 120.400 0.056 0.000 2.472 16 D HA 0.227 4.867 4.640 0.000 0.000 0.237 16 D C 1.649 178.011 176.300 0.104 0.000 1.141 16 D CA 1.840 55.885 54.000 0.074 0.000 0.875 16 D CB -0.183 40.646 40.800 0.048 0.000 1.192 16 D HN 0.713 nan 8.370 nan 0.000 0.450 17 G N 1.626 110.494 108.800 0.114 0.000 2.179 17 G HA2 -0.372 3.588 3.960 0.000 0.000 0.260 17 G HA3 -0.372 3.588 3.960 0.000 0.000 0.260 17 G C 0.870 175.866 174.900 0.160 0.000 0.977 17 G CA 0.291 45.456 45.100 0.110 0.000 0.641 17 G HN 0.467 nan 8.290 nan 0.000 0.533 18 F N 0.997 120.968 119.950 0.034 0.000 2.102 18 F HA 0.151 4.678 4.527 0.001 0.000 0.298 18 F C 2.526 178.360 175.800 0.057 0.000 1.105 18 F CA 2.064 60.090 58.000 0.043 0.000 1.239 18 F CB -0.357 38.672 39.000 0.048 0.000 0.991 18 F HN 0.209 nan 8.300 nan 0.000 0.474 19 L N -1.023 120.219 121.223 0.031 0.000 2.083 19 L HA -0.226 4.114 4.340 0.000 0.000 0.209 19 L C 2.721 179.548 176.870 -0.072 0.000 1.083 19 L CA 1.562 56.365 54.840 -0.062 0.000 0.752 19 L CB -1.020 41.065 42.059 0.044 0.000 0.899 19 L HN 0.199 nan 8.230 nan 0.000 0.433 20 S N -0.361 115.327 115.700 -0.020 0.000 2.383 20 S HA -0.244 4.226 4.470 0.000 0.000 0.227 20 S C 1.933 176.506 174.600 -0.045 0.000 1.026 20 S CA 1.640 59.830 58.200 -0.018 0.000 0.981 20 S CB -0.054 63.152 63.200 0.010 0.000 0.818 20 S HN 0.468 nan 8.310 nan 0.000 0.472 21 E N 0.287 120.451 120.200 -0.060 0.000 2.072 21 E HA -0.092 4.258 4.350 0.000 0.000 0.191 21 E C 2.108 178.623 176.600 -0.142 0.000 0.985 21 E CA 1.095 57.453 56.400 -0.070 0.000 0.801 21 E CB -0.216 29.471 29.700 -0.021 0.000 0.750 21 E HN 0.533 nan 8.360 nan 0.000 0.452 22 L N 0.443 121.509 121.223 -0.261 0.000 2.046 22 L HA -0.189 4.152 4.340 0.000 0.000 0.208 22 L C 2.666 179.440 176.870 -0.160 0.000 1.077 22 L CA 1.647 56.320 54.840 -0.278 0.000 0.747 22 L CB -0.605 41.248 42.059 -0.344 0.000 0.896 22 L HN 0.238 nan 8.230 nan 0.000 0.432 23 T N -0.874 113.618 114.554 -0.103 0.000 2.684 23 T HA -0.259 4.091 4.350 0.000 0.000 0.267 23 T C 1.890 176.559 174.700 -0.052 0.000 1.036 23 T CA 1.502 63.570 62.100 -0.054 0.000 1.148 23 T CB -0.206 68.646 68.868 -0.027 0.000 0.863 23 T HN 0.394 nan 8.240 nan 0.000 0.436 24 Q N 0.286 120.056 119.800 -0.050 0.000 2.046 24 Q HA -0.095 4.245 4.340 0.000 0.000 0.200 24 Q C 2.746 178.720 176.000 -0.045 0.000 0.975 24 Q CA 0.957 56.738 55.803 -0.036 0.000 0.836 24 Q CB -0.111 28.612 28.738 -0.026 0.000 0.896 24 Q HN 0.420 nan 8.270 nan 0.000 0.428 25 Q N 0.476 120.236 119.800 -0.065 0.000 2.119 25 Q HA -0.086 4.254 4.340 0.000 0.000 0.201 25 Q C 2.181 178.133 176.000 -0.080 0.000 0.972 25 Q CA 1.078 56.841 55.803 -0.067 0.000 0.847 25 Q CB -0.171 28.517 28.738 -0.084 0.000 0.903 25 Q HN 0.424 nan 8.270 nan 0.000 0.433 26 L N 0.025 121.177 121.223 -0.119 0.000 2.141 26 L HA -0.114 4.226 4.340 0.000 0.000 0.209 26 L C 2.383 179.200 176.870 -0.088 0.000 1.094 26 L CA 0.853 55.607 54.840 -0.144 0.000 0.763 26 L CB -0.517 41.438 42.059 -0.174 0.000 0.908 26 L HN 0.111 nan 8.230 nan 0.000 0.437 27 A N -0.844 121.943 122.820 -0.055 0.000 1.898 27 A HA -0.212 4.108 4.320 0.000 0.000 0.216 27 A C 2.264 179.836 177.584 -0.020 0.000 1.181 27 A CA 1.222 53.242 52.037 -0.029 0.000 0.620 27 A CB -0.363 18.629 19.000 -0.012 0.000 0.819 27 A HN 0.328 nan 8.150 nan 0.000 0.442 28 Q N -0.527 119.263 119.800 -0.017 0.000 2.050 28 Q HA -0.119 4.221 4.340 0.000 0.000 0.202 28 Q C 2.461 178.470 176.000 0.015 0.000 0.980 28 Q CA 1.717 57.517 55.803 -0.004 0.000 0.840 28 Q CB -0.650 28.084 28.738 -0.006 0.000 0.898 28 Q HN 0.635 nan 8.270 nan 0.000 0.424 29 A N 0.491 123.326 122.820 0.026 0.000 1.898 29 A HA -0.145 4.175 4.320 0.000 0.000 0.216 29 A C 2.219 179.911 177.584 0.180 0.000 1.181 29 A CA 2.283 54.387 52.037 0.112 0.000 0.620 29 A CB -0.764 18.321 19.000 0.141 0.000 0.819 29 A HN 0.534 nan 8.150 nan 0.000 0.442 30 T N -4.625 109.972 114.554 0.071 0.000 3.067 30 T HA 0.375 4.725 4.350 0.000 0.000 0.257 30 T C 1.492 176.208 174.700 0.026 0.000 1.105 30 T CA 1.150 63.291 62.100 0.068 0.000 1.104 30 T CB 0.004 68.840 68.868 -0.054 0.000 0.925 30 T HN 1.734 nan 8.240 nan 0.000 0.498 31 G N 1.715 110.519 108.800 0.006 0.000 2.162 31 G HA2 -0.240 3.720 3.960 0.000 0.000 0.260 31 G HA3 -0.240 3.720 3.960 0.000 0.000 0.260 31 G C -0.004 174.872 174.900 -0.040 0.000 0.976 31 G CA 0.254 45.346 45.100 -0.014 0.000 0.655 31 G HN 0.666 nan 8.290 nan 0.000 0.533 32 K N 0.592 120.960 120.400 -0.054 0.000 2.098 32 K HA 0.463 4.783 4.320 0.000 0.000 0.261 32 K C -2.608 173.978 176.600 -0.023 0.000 0.987 32 K CA -1.937 54.297 56.287 -0.088 0.000 0.916 32 K CB 1.255 33.675 32.500 -0.134 0.000 1.039 32 K HN -0.020 nan 8.250 nan 0.000 0.455 33 P HA -0.009 nan 4.420 nan 0.000 0.267 33 P C -1.823 175.545 177.300 0.114 0.000 1.200 33 P CA -0.975 62.184 63.100 0.098 0.000 0.772 33 P CB 0.168 31.988 31.700 0.200 0.000 0.855 34 P HA -0.171 nan 4.420 nan 0.000 0.222 34 P C 1.047 178.366 177.300 0.033 0.000 1.147 34 P CA 1.438 64.561 63.100 0.037 0.000 0.790 34 P CB 0.155 31.865 31.700 0.016 0.000 0.780 35 Q N -1.008 118.806 119.800 0.025 0.000 2.234 35 Q HA -0.148 4.192 4.340 0.000 0.000 0.206 35 Q C 1.653 177.548 176.000 -0.175 0.000 0.980 35 Q CA 1.500 57.254 55.803 -0.082 0.000 0.869 35 Q CB -0.879 27.769 28.738 -0.150 0.000 0.912 35 Q HN 0.433 nan 8.270 nan 0.000 0.436 36 Y N -0.731 119.527 120.300 -0.070 0.000 2.482 36 Y HA 0.091 4.642 4.550 0.000 0.000 0.270 36 Y C 0.234 176.085 175.900 -0.082 0.000 1.152 36 Y CA -0.443 57.586 58.100 -0.118 0.000 1.292 36 Y CB 0.634 38.980 38.460 -0.190 0.000 1.070 36 Y HN -0.001 nan 8.280 nan 0.000 0.528 37 I N 1.173 121.789 120.570 0.077 0.000 2.416 37 I HA 0.294 4.464 4.170 0.000 0.000 0.288 37 I C 0.362 176.507 176.117 0.048 0.000 1.051 37 I CA -0.576 60.753 61.300 0.049 0.000 1.375 37 I CB 0.279 38.293 38.000 0.024 0.000 1.407 37 I HN -0.054 nan 8.210 nan 0.000 0.516 38 A N 7.203 130.063 122.820 0.067 0.000 2.342 38 A HA 0.799 5.119 4.320 0.000 0.000 0.323 38 A C -0.473 177.174 177.584 0.105 0.000 1.125 38 A CA -0.496 51.584 52.037 0.072 0.000 0.785 38 A CB 1.682 20.725 19.000 0.072 0.000 1.221 38 A HN 0.593 nan 8.150 nan 0.000 0.463 39 V N 0.074 120.049 119.914 0.102 0.000 2.876 39 V HA 0.773 4.893 4.120 0.000 0.000 0.312 39 V C -0.700 175.494 176.094 0.166 0.000 1.085 39 V CA -0.532 61.857 62.300 0.149 0.000 0.945 39 V CB 1.542 33.436 31.823 0.119 0.000 1.017 39 V HN 1.087 nan 8.190 nan 0.000 0.428 40 H N 1.802 120.881 119.070 0.015 0.000 3.017 40 H HA 0.764 5.320 4.556 0.000 0.000 0.340 40 H C -2.116 173.206 175.328 -0.011 0.000 1.014 40 H CA -0.615 55.431 56.048 -0.003 0.000 1.341 40 H CB 2.329 32.074 29.762 -0.028 0.000 1.739 40 H HN 0.718 nan 8.280 nan 0.000 0.506 41 V N 6.117 126.139 119.914 0.179 0.000 2.448 41 V HA 0.255 4.375 4.120 0.000 0.000 0.295 41 V C -0.424 175.686 176.094 0.027 0.000 1.025 41 V CA -0.716 61.634 62.300 0.083 0.000 0.859 41 V CB 1.678 33.651 31.823 0.249 0.000 0.988 41 V HN 0.533 nan 8.190 nan 0.000 0.431 42 V N 7.512 127.375 119.914 -0.084 0.000 2.305 42 V HA 0.385 4.505 4.120 0.000 0.000 0.275 42 V C -2.076 174.004 176.094 -0.024 0.000 1.020 42 V CA -1.279 60.984 62.300 -0.063 0.000 0.811 42 V CB 1.362 33.092 31.823 -0.156 0.000 1.031 42 V HN 0.713 nan 8.190 nan 0.000 0.439 43 P HA 0.348 nan 4.420 nan 0.000 0.297 43 P C -0.246 177.053 177.300 -0.002 0.000 1.307 43 P CA -0.100 62.999 63.100 -0.001 0.000 0.773 43 P CB 0.822 32.523 31.700 0.001 0.000 1.265 44 D N -2.636 117.761 120.400 -0.006 0.000 2.911 44 D HA -0.121 4.519 4.640 0.000 0.000 0.227 44 D C -0.027 176.273 176.300 -0.000 0.000 1.164 44 D CA 1.052 55.051 54.000 -0.003 0.000 0.782 44 D CB -0.748 40.052 40.800 0.001 0.000 1.094 44 D HN 0.386 nan 8.370 nan 0.000 0.425 45 Q N -0.042 119.757 119.800 -0.002 0.000 2.306 45 Q HA 0.367 4.707 4.340 0.000 0.000 0.241 45 Q C 0.733 176.736 176.000 0.005 0.000 0.948 45 Q CA -0.338 55.465 55.803 0.001 0.000 0.886 45 Q CB 1.224 29.961 28.738 -0.002 0.000 1.227 45 Q HN 0.363 nan 8.270 nan 0.000 0.457 46 L N 4.598 125.825 121.223 0.008 0.000 2.302 46 L HA 0.310 4.650 4.340 0.000 0.000 0.285 46 L C -0.485 176.394 176.870 0.015 0.000 1.090 46 L CA 0.048 54.894 54.840 0.010 0.000 0.866 46 L CB -0.185 41.880 42.059 0.009 0.000 1.244 46 L HN 0.606 nan 8.230 nan 0.000 0.435 47 M N 3.419 123.031 119.600 0.021 0.000 2.622 47 M HA 0.886 5.367 4.480 0.000 0.000 0.276 47 M C -1.451 174.875 176.300 0.043 0.000 1.265 47 M CA -0.724 54.596 55.300 0.033 0.000 0.850 47 M CB 2.268 34.893 32.600 0.042 0.000 1.720 47 M HN 0.241 nan 8.290 nan 0.000 0.465 48 A N 1.077 123.930 122.820 0.056 0.000 2.539 48 A HA 0.902 5.222 4.320 0.000 0.000 0.296 48 A C -2.234 175.419 177.584 0.116 0.000 1.073 48 A CA -0.586 51.495 52.037 0.073 0.000 0.700 48 A CB 1.786 20.812 19.000 0.043 0.000 1.296 48 A HN 0.903 nan 8.150 nan 0.000 0.405 49 F N 1.309 121.237 119.950 -0.037 0.000 2.539 49 F HA 0.529 5.056 4.527 0.000 0.000 0.328 49 F C 1.013 176.784 175.800 -0.048 0.000 1.148 49 F CA 0.740 58.701 58.000 -0.065 0.000 0.940 49 F CB 1.842 40.764 39.000 -0.129 0.000 1.194 49 F HN 1.474 nan 8.300 nan 0.000 0.438 50 G N 3.265 112.043 108.800 -0.036 0.000 2.179 50 G HA2 -0.035 3.925 3.960 0.000 0.000 0.257 50 G HA3 -0.035 3.925 3.960 0.000 0.000 0.257 50 G C 1.017 175.951 174.900 0.058 0.000 1.010 50 G CA 0.470 45.601 45.100 0.050 0.000 0.736 50 G HN 2.198 nan 8.290 nan 0.000 0.513 51 G N -2.041 106.782 108.800 0.038 0.000 2.143 51 G HA2 0.090 4.050 3.960 0.000 0.000 0.249 51 G HA3 0.090 4.050 3.960 0.000 0.000 0.249 51 G C 0.406 175.333 174.900 0.044 0.000 0.981 51 G CA 1.427 46.546 45.100 0.033 0.000 0.665 51 G HN 2.239 nan 8.290 nan 0.000 0.528 52 S N -0.663 115.078 115.700 0.068 0.000 2.536 52 S HA 0.713 5.184 4.470 0.000 0.000 0.298 52 S C 1.092 175.724 174.600 0.053 0.000 1.083 52 S CA 0.707 58.939 58.200 0.054 0.000 0.995 52 S CB 1.719 64.950 63.200 0.052 0.000 1.058 52 S HN 1.305 nan 8.310 nan 0.000 0.488 53 S N 2.078 117.796 115.700 0.030 0.000 2.582 53 S HA 0.298 4.769 4.470 0.000 0.000 0.234 53 S C 0.246 174.850 174.600 0.006 0.000 0.961 53 S CA -0.516 57.697 58.200 0.022 0.000 0.953 53 S CB -0.167 63.042 63.200 0.015 0.000 0.800 53 S HN 0.676 nan 8.310 nan 0.000 0.471 54 E N 2.841 123.040 120.200 -0.001 0.000 2.410 54 E HA 0.270 4.620 4.350 0.000 0.000 0.255 54 E C -2.555 174.022 176.600 -0.038 0.000 1.194 54 E CA -2.104 54.282 56.400 -0.023 0.000 0.955 54 E CB -0.313 29.367 29.700 -0.034 0.000 0.988 54 E HN 0.153 nan 8.360 nan 0.000 0.461 55 P HA -0.073 nan 4.420 nan 0.000 0.262 55 P C -0.574 176.666 177.300 -0.100 0.000 1.182 55 P CA 0.417 63.479 63.100 -0.063 0.000 0.761 55 P CB 0.274 31.935 31.700 -0.066 0.000 0.795 56 C N 1.459 120.710 119.300 -0.082 0.000 3.332 56 C HA 0.953 5.413 4.460 0.000 0.000 0.329 56 C C -1.210 173.751 174.990 -0.049 0.000 1.434 56 C CA -1.016 57.928 59.018 -0.123 0.000 1.314 56 C CB 1.207 28.896 27.740 -0.084 0.000 1.664 56 C HN 0.652 nan 8.230 nan 0.000 0.457 57 A N 0.840 123.638 122.820 -0.035 0.000 2.547 57 A HA 0.815 5.135 4.320 0.000 0.000 0.297 57 A C -1.583 176.052 177.584 0.084 0.000 1.056 57 A CA -0.446 51.607 52.037 0.027 0.000 0.688 57 A CB 1.023 20.046 19.000 0.038 0.000 1.282 57 A HN 1.111 nan 8.150 nan 0.000 0.400 58 L N 1.691 122.962 121.223 0.081 0.000 2.319 58 L HA 0.563 4.903 4.340 0.000 0.000 0.281 58 L C -0.748 176.139 176.870 0.029 0.000 1.005 58 L CA -0.497 54.412 54.840 0.115 0.000 0.828 58 L CB 1.414 43.541 42.059 0.113 0.000 1.227 58 L HN 0.807 nan 8.230 nan 0.000 0.415 59 C N 0.961 120.279 119.300 0.031 0.000 2.913 59 C HA 0.889 5.349 4.460 0.000 0.000 0.322 59 C C 0.122 175.065 174.990 -0.078 0.000 1.292 59 C CA -0.800 58.161 59.018 -0.095 0.000 1.649 59 C CB 2.006 29.729 27.740 -0.028 0.000 2.139 59 C HN 0.841 nan 8.230 nan 0.000 0.475 60 S N 0.366 115.979 115.700 -0.145 0.000 2.536 60 S HA 0.776 5.246 4.470 0.000 0.000 0.271 60 S C -1.543 173.092 174.600 0.058 0.000 1.134 60 S CA -0.530 57.657 58.200 -0.020 0.000 0.897 60 S CB 1.497 64.801 63.200 0.173 0.000 1.094 60 S HN 0.826 nan 8.310 nan 0.000 0.473 61 L N 2.586 123.832 121.223 0.040 0.000 2.372 61 L HA 0.654 4.995 4.340 0.000 0.000 0.274 61 L C -1.657 175.310 176.870 0.161 0.000 0.988 61 L CA -0.132 54.834 54.840 0.210 0.000 0.833 61 L CB 1.137 43.321 42.059 0.207 0.000 1.236 61 L HN 0.960 nan 8.230 nan 0.000 0.410 62 H N 2.480 121.684 119.070 0.223 0.000 2.457 62 H HA 0.840 5.396 4.556 0.000 0.000 0.335 62 H C -0.652 174.752 175.328 0.126 0.000 1.115 62 H CA -0.324 55.853 56.048 0.215 0.000 1.219 62 H CB 1.910 31.753 29.762 0.135 0.000 1.471 62 H HN 0.588 nan 8.280 nan 0.000 0.491 63 S N 2.004 117.833 115.700 0.215 0.000 2.537 63 S HA 0.422 4.892 4.470 0.000 0.000 0.270 63 S C -1.117 173.522 174.600 0.065 0.000 1.142 63 S CA -0.783 57.473 58.200 0.094 0.000 0.870 63 S CB 0.770 64.006 63.200 0.061 0.000 1.112 63 S HN 0.559 nan 8.310 nan 0.000 0.466 64 I N 4.292 124.848 120.570 -0.023 0.000 2.269 64 I HA 0.496 4.666 4.170 0.000 0.000 0.293 64 I C 1.025 177.118 176.117 -0.039 0.000 1.106 64 I CA 0.529 61.807 61.300 -0.037 0.000 1.248 64 I CB -0.124 37.798 38.000 -0.129 0.000 1.444 64 I HN 1.021 nan 8.210 nan 0.000 0.497 65 G N 5.533 114.337 108.800 0.007 0.000 2.760 65 G HA2 -0.217 3.743 3.960 0.000 0.000 0.246 65 G HA3 -0.217 3.743 3.960 0.000 0.000 0.246 65 G C 0.273 175.190 174.900 0.028 0.000 1.359 65 G CA -0.661 44.442 45.100 0.006 0.000 0.861 65 G HN 0.574 nan 8.290 nan 0.000 0.541 66 K N -2.148 118.269 120.400 0.029 0.000 3.281 66 K HA -0.167 4.153 4.320 0.000 0.000 0.295 66 K C 0.350 177.038 176.600 0.146 0.000 1.233 66 K CA 1.665 57.998 56.287 0.076 0.000 0.866 66 K CB -1.510 31.049 32.500 0.099 0.000 1.265 66 K HN 0.876 nan 8.250 nan 0.000 0.482 67 I N 0.399 121.015 120.570 0.078 0.000 2.436 67 I HA 0.650 4.821 4.170 0.000 0.000 0.289 67 I C 0.894 176.990 176.117 -0.036 0.000 1.010 67 I CA -0.179 61.159 61.300 0.063 0.000 1.098 67 I CB 1.715 39.770 38.000 0.092 0.000 1.266 67 I HN 0.246 nan 8.210 nan 0.000 0.434 68 G N 2.988 111.714 108.800 -0.123 0.000 2.451 68 G HA2 0.430 4.391 3.960 0.000 0.000 0.292 68 G HA3 0.430 4.391 3.960 0.000 0.000 0.292 68 G C 0.428 175.217 174.900 -0.186 0.000 1.427 68 G CA -0.077 44.946 45.100 -0.129 0.000 0.792 68 G HN 0.689 nan 8.290 nan 0.000 0.498 69 G N 0.249 108.966 108.800 -0.139 0.000 2.586 69 G HA2 0.074 4.034 3.960 0.000 0.000 0.218 69 G HA3 0.074 4.034 3.960 0.000 0.000 0.218 69 G C 2.060 176.853 174.900 -0.179 0.000 1.216 69 G CA 2.834 47.851 45.100 -0.139 0.000 0.786 69 G HN 1.657 nan 8.290 nan 0.000 0.583 70 A N -0.041 122.677 122.820 -0.170 0.000 1.898 70 A HA -0.037 4.283 4.320 0.000 0.000 0.216 70 A C 2.386 179.795 177.584 -0.292 0.000 1.181 70 A CA 1.977 53.902 52.037 -0.187 0.000 0.620 70 A CB -0.447 18.466 19.000 -0.145 0.000 0.819 70 A HN 0.509 nan 8.150 nan 0.000 0.442 71 Q N -0.298 119.282 119.800 -0.367 0.000 2.124 71 Q HA -0.173 4.167 4.340 0.000 0.000 0.202 71 Q C 1.761 177.176 176.000 -0.976 0.000 0.977 71 Q CA 1.481 56.893 55.803 -0.652 0.000 0.850 71 Q CB -0.243 28.155 28.738 -0.566 0.000 0.901 71 Q HN 0.617 nan 8.270 nan 0.000 0.429 72 N N 0.533 118.819 118.700 -0.690 0.000 2.188 72 N HA -0.095 4.646 4.740 0.000 0.000 0.184 72 N C 1.508 176.740 175.510 -0.464 0.000 1.018 72 N CA 0.985 53.562 53.050 -0.788 0.000 0.858 72 N CB -0.121 37.895 38.487 -0.786 0.000 0.989 72 N HN 0.185 nan 8.380 nan 0.000 0.426 73 R N -0.001 120.315 120.500 -0.307 0.000 2.092 73 R HA 0.008 4.348 4.340 0.000 0.000 0.231 73 R C 2.231 178.451 176.300 -0.135 0.000 1.119 73 R CA 1.094 57.102 56.100 -0.153 0.000 0.970 73 R CB -0.349 29.879 30.300 -0.121 0.000 0.864 73 R HN 0.138 nan 8.270 nan 0.000 0.440 74 S N -0.070 115.487 115.700 -0.238 0.000 2.368 74 S HA -0.125 4.345 4.470 0.000 0.000 0.224 74 S C 1.798 176.367 174.600 -0.052 0.000 1.029 74 S CA 1.016 59.111 58.200 -0.175 0.000 0.988 74 S CB -0.202 62.844 63.200 -0.256 0.000 0.838 74 S HN 0.394 nan 8.310 nan 0.000 0.462 75 Y N 1.247 121.507 120.300 -0.068 0.000 2.224 75 Y HA -0.106 4.444 4.550 0.001 0.000 0.289 75 Y C 3.030 178.976 175.900 0.077 0.000 1.146 75 Y CA 0.806 58.905 58.100 -0.001 0.000 1.182 75 Y CB -0.306 38.181 38.460 0.046 0.000 0.983 75 Y HN 0.279 nan 8.280 nan 0.000 0.524 76 S N 0.024 115.881 115.700 0.261 0.000 2.383 76 S HA -0.186 4.284 4.470 0.000 0.000 0.227 76 S C 1.911 176.590 174.600 0.130 0.000 1.026 76 S CA 1.203 59.547 58.200 0.241 0.000 0.981 76 S CB -0.208 63.127 63.200 0.226 0.000 0.818 76 S HN 0.351 nan 8.310 nan 0.000 0.472 77 K N 0.829 121.277 120.400 0.079 0.000 2.057 77 K HA -0.080 4.240 4.320 0.000 0.000 0.206 77 K C 2.184 178.812 176.600 0.047 0.000 1.050 77 K CA 1.071 57.387 56.287 0.048 0.000 0.935 77 K CB -0.266 32.246 32.500 0.020 0.000 0.715 77 K HN 0.237 nan 8.250 nan 0.000 0.439 78 L N 1.539 122.794 121.223 0.053 0.000 1.994 78 L HA -0.144 4.197 4.340 0.000 0.000 0.208 78 L C 2.013 178.889 176.870 0.011 0.000 1.071 78 L CA 1.594 56.453 54.840 0.032 0.000 0.745 78 L CB -0.409 41.674 42.059 0.040 0.000 0.892 78 L HN 0.208 nan 8.230 nan 0.000 0.431 79 L N -1.505 119.722 121.223 0.007 0.000 2.056 79 L HA -0.233 4.107 4.340 0.000 0.000 0.207 79 L C 2.604 179.508 176.870 0.058 0.000 1.078 79 L CA 1.214 56.034 54.840 -0.034 0.000 0.749 79 L CB -0.766 41.234 42.059 -0.099 0.000 0.901 79 L HN 0.386 nan 8.230 nan 0.000 0.433 80 C N 0.079 119.425 119.300 0.077 0.000 2.425 80 C HA -0.077 4.383 4.460 0.000 0.000 0.277 80 C C 2.921 177.944 174.990 0.055 0.000 1.280 80 C CA 0.778 59.840 59.018 0.072 0.000 1.744 80 C CB -1.513 26.265 27.740 0.063 0.000 1.989 80 C HN 0.706 nan 8.230 nan 0.000 0.491 81 G N 0.465 109.293 108.800 0.047 0.000 2.446 81 G HA2 -0.191 3.769 3.960 0.000 0.000 0.217 81 G HA3 -0.191 3.769 3.960 0.000 0.000 0.217 81 G C 1.557 176.491 174.900 0.056 0.000 1.168 81 G CA 0.713 45.837 45.100 0.039 0.000 0.771 81 G HN 0.524 nan 8.290 nan 0.000 0.551 82 L N -0.181 121.087 121.223 0.075 0.000 2.056 82 L HA 0.054 4.394 4.340 0.000 0.000 0.207 82 L C 2.904 179.889 176.870 0.193 0.000 1.078 82 L CA 0.439 55.361 54.840 0.136 0.000 0.749 82 L CB -0.346 41.787 42.059 0.125 0.000 0.901 82 L HN 0.176 nan 8.230 nan 0.000 0.433 83 L N -0.538 120.789 121.223 0.173 0.000 2.083 83 L HA -0.188 4.152 4.340 0.000 0.000 0.209 83 L C 2.826 179.724 176.870 0.047 0.000 1.083 83 L CA 1.092 56.019 54.840 0.145 0.000 0.752 83 L CB -0.671 41.471 42.059 0.139 0.000 0.899 83 L HN 0.251 nan 8.230 nan 0.000 0.433 84 A N -0.185 122.656 122.820 0.035 0.000 1.872 84 A HA -0.231 4.089 4.320 0.000 0.000 0.214 84 A C 2.273 179.857 177.584 0.000 0.000 1.187 84 A CA 1.649 53.687 52.037 0.003 0.000 0.614 84 A CB -0.454 18.546 19.000 0.001 0.000 0.826 84 A HN 0.441 nan 8.150 nan 0.000 0.442 85 E N -0.812 119.401 120.200 0.021 0.000 2.072 85 E HA -0.198 4.153 4.350 0.000 0.000 0.191 85 E C 2.139 178.741 176.600 0.003 0.000 0.985 85 E CA 0.961 57.372 56.400 0.018 0.000 0.801 85 E CB -0.001 29.721 29.700 0.036 0.000 0.750 85 E HN 0.333 nan 8.360 nan 0.000 0.452 86 R N -0.145 120.361 120.500 0.010 0.000 2.112 86 R HA 0.103 4.443 4.340 0.000 0.000 0.216 86 R C 2.114 178.301 176.300 -0.188 0.000 1.080 86 R CA 0.647 56.704 56.100 -0.072 0.000 0.996 86 R CB -0.159 30.113 30.300 -0.046 0.000 0.902 86 R HN 0.301 nan 8.270 nan 0.000 0.449 87 L N 0.737 121.855 121.223 -0.175 0.000 2.766 87 L HA 0.262 4.602 4.340 0.000 0.000 0.242 87 L C -0.178 176.631 176.870 -0.101 0.000 1.136 87 L CA -0.306 54.425 54.840 -0.182 0.000 0.933 87 L CB 0.287 42.224 42.059 -0.204 0.000 1.241 87 L HN -0.050 nan 8.230 nan 0.000 0.522 88 R N 0.490 120.948 120.500 -0.070 0.000 3.531 88 R HA -0.129 4.211 4.340 0.000 0.000 0.280 88 R C -0.524 175.742 176.300 -0.057 0.000 1.130 88 R CA 0.675 56.742 56.100 -0.054 0.000 0.757 88 R CB -3.007 27.261 30.300 -0.053 0.000 1.218 88 R HN 0.342 nan 8.270 nan 0.000 0.454 89 I N 0.758 121.295 120.570 -0.055 0.000 2.336 89 I HA 0.136 4.307 4.170 0.000 0.000 0.292 89 I C 0.697 176.768 176.117 -0.076 0.000 0.991 89 I CA -0.470 60.788 61.300 -0.071 0.000 1.227 89 I CB 1.706 39.668 38.000 -0.063 0.000 1.366 89 I HN -0.042 nan 8.210 nan 0.000 0.466 90 S N 7.925 123.562 115.700 -0.106 0.000 2.533 90 S HA 0.070 4.541 4.470 0.000 0.000 0.282 90 S C -1.527 172.994 174.600 -0.131 0.000 1.304 90 S CA -0.811 57.325 58.200 -0.108 0.000 1.063 90 S CB 0.809 63.934 63.200 -0.125 0.000 0.881 90 S HN 0.467 nan 8.310 nan 0.000 0.493 91 P HA -0.158 nan 4.420 nan 0.000 0.217 91 P C 0.855 178.091 177.300 -0.107 0.000 1.148 91 P CA 1.038 64.100 63.100 -0.062 0.000 0.828 91 P CB -0.005 31.684 31.700 -0.018 0.000 0.783 92 D N -1.621 118.694 120.400 -0.141 0.000 2.349 92 D HA -0.082 4.558 4.640 0.000 0.000 0.224 92 D C 0.931 176.943 176.300 -0.480 0.000 1.029 92 D CA 0.424 54.323 54.000 -0.169 0.000 0.879 92 D CB -0.565 40.186 40.800 -0.082 0.000 0.906 92 D HN 0.169 nan 8.370 nan 0.000 0.528 93 R N 0.015 120.112 120.500 -0.673 0.000 2.700 93 R HA 0.351 4.691 4.340 0.000 0.000 0.377 93 R C -0.938 174.749 176.300 -1.021 0.000 1.130 93 R CA -0.246 55.015 56.100 -1.398 0.000 1.055 93 R CB 1.403 31.204 30.300 -0.830 0.000 1.387 93 R HN -0.013 nan 8.270 nan 0.000 0.580 94 V N 0.809 120.378 119.914 -0.574 0.000 2.638 94 V HA 0.395 4.515 4.120 0.000 0.000 0.306 94 V C -0.884 175.260 176.094 0.084 0.000 1.052 94 V CA -0.806 61.399 62.300 -0.158 0.000 0.885 94 V CB 2.165 33.971 31.823 -0.028 0.000 0.999 94 V HN 0.201 nan 8.190 nan 0.000 0.424 95 Y N 4.399 124.826 120.300 0.211 0.000 2.409 95 Y HA 0.690 5.241 4.550 0.001 0.000 0.343 95 Y C -0.143 175.795 175.900 0.064 0.000 0.973 95 Y CA -1.050 57.149 58.100 0.166 0.000 1.064 95 Y CB 2.400 40.962 38.460 0.169 0.000 1.207 95 Y HN 0.452 nan 8.280 nan 0.000 0.452 96 I N 3.526 124.193 120.570 0.162 0.000 2.468 96 I HA 0.228 4.399 4.170 0.000 0.000 0.284 96 I C -1.089 174.826 176.117 -0.336 0.000 1.038 96 I CA -0.501 60.744 61.300 -0.090 0.000 1.083 96 I CB 1.558 39.461 38.000 -0.162 0.000 1.223 96 I HN 0.613 nan 8.210 nan 0.000 0.443 97 N N 5.594 124.103 118.700 -0.319 0.000 2.444 97 N HA 0.381 5.121 4.740 0.000 0.000 0.271 97 N C -1.398 173.669 175.510 -0.737 0.000 1.069 97 N CA -0.419 52.359 53.050 -0.454 0.000 0.965 97 N CB 0.898 39.215 38.487 -0.283 0.000 1.092 97 N HN 0.394 nan 8.380 nan 0.000 0.476 98 Y N 1.879 121.886 120.300 -0.488 0.000 2.342 98 Y HA 0.340 4.891 4.550 0.001 0.000 0.334 98 Y C -0.710 174.804 175.900 -0.644 0.000 1.067 98 Y CA -0.586 57.293 58.100 -0.368 0.000 1.128 98 Y CB 0.871 39.259 38.460 -0.120 0.000 1.200 98 Y HN 0.433 nan 8.280 nan 0.000 0.464 99 Y N 1.375 121.677 120.300 0.003 0.000 2.346 99 Y HA 0.213 4.763 4.550 0.000 0.000 0.332 99 Y C -0.654 175.282 175.900 0.059 0.000 0.985 99 Y CA -1.409 56.676 58.100 -0.025 0.000 1.112 99 Y CB 1.442 39.791 38.460 -0.184 0.000 1.170 99 Y HN 0.500 nan 8.280 nan 0.000 0.447 100 D N 4.465 124.979 120.400 0.190 0.000 2.380 100 D HA 0.225 4.865 4.640 0.000 0.000 0.230 100 D C -0.550 175.840 176.300 0.151 0.000 1.154 100 D CA -0.147 53.938 54.000 0.141 0.000 0.859 100 D CB 0.581 41.434 40.800 0.089 0.000 1.045 100 D HN 0.339 nan 8.370 nan 0.000 0.495 101 M N 2.250 121.942 119.600 0.154 0.000 2.314 101 M HA 0.266 4.746 4.480 0.000 0.000 0.342 101 M C 0.464 176.822 176.300 0.096 0.000 1.171 101 M CA -0.427 54.958 55.300 0.141 0.000 1.098 101 M CB 0.739 33.435 32.600 0.159 0.000 1.559 101 M HN 0.223 nan 8.290 nan 0.000 0.459 102 N N 0.419 119.174 118.700 0.093 0.000 2.529 102 N HA 0.297 5.037 4.740 0.000 0.000 0.278 102 N C 0.786 176.355 175.510 0.098 0.000 1.146 102 N CA -0.274 52.823 53.050 0.078 0.000 0.980 102 N CB 1.446 39.976 38.487 0.072 0.000 1.124 102 N HN 0.753 nan 8.380 nan 0.000 0.458 103 A N 2.209 125.086 122.820 0.096 0.000 1.978 103 A HA -0.174 4.146 4.320 0.000 0.000 0.220 103 A C 2.021 179.716 177.584 0.186 0.000 1.170 103 A CA 1.912 54.042 52.037 0.156 0.000 0.636 103 A CB -0.588 18.496 19.000 0.140 0.000 0.810 103 A HN 0.775 nan 8.150 nan 0.000 0.448 104 A N -0.554 122.341 122.820 0.125 0.000 2.119 104 A HA -0.039 4.281 4.320 0.000 0.000 0.217 104 A C 1.382 179.024 177.584 0.096 0.000 1.153 104 A CA 1.177 53.276 52.037 0.103 0.000 0.692 104 A CB -0.295 18.748 19.000 0.073 0.000 0.799 104 A HN 0.473 nan 8.150 nan 0.000 0.458 105 N N -0.309 118.454 118.700 0.105 0.000 2.251 105 N HA 0.199 4.939 4.740 0.000 0.000 0.217 105 N C -0.874 174.703 175.510 0.112 0.000 1.124 105 N CA 0.303 53.407 53.050 0.091 0.000 0.843 105 N CB 1.028 39.562 38.487 0.079 0.000 1.024 105 N HN 0.133 nan 8.380 nan 0.000 0.501 106 V N 0.226 120.241 119.914 0.168 0.000 2.409 106 V HA 0.567 4.688 4.120 0.000 0.000 0.290 106 V C 0.576 176.789 176.094 0.197 0.000 1.017 106 V CA -1.139 61.296 62.300 0.226 0.000 0.841 106 V CB 1.436 33.486 31.823 0.378 0.000 1.003 106 V HN 0.112 nan 8.190 nan 0.000 0.426 107 G N 3.166 112.038 108.800 0.120 0.000 2.395 107 G HA2 0.542 4.502 3.960 0.000 0.000 0.283 107 G HA3 0.542 4.502 3.960 0.000 0.000 0.283 107 G C -1.450 173.511 174.900 0.101 0.000 1.178 107 G CA -0.321 44.812 45.100 0.054 0.000 0.837 107 G HN 0.846 nan 8.290 nan 0.000 0.518 108 W N 2.159 123.303 121.300 -0.260 0.000 3.645 108 W HA 0.380 5.040 4.660 0.000 0.000 0.285 108 W C -0.106 176.261 176.519 -0.253 0.000 1.266 108 W CA -0.851 56.328 57.345 -0.276 0.000 1.212 108 W CB 0.500 29.657 29.460 -0.505 0.000 1.306 108 W HN 0.803 nan 8.180 nan 0.000 0.552 109 N N 4.765 122.817 118.700 -1.079 0.000 2.705 109 N HA -0.331 4.409 4.740 0.000 0.000 0.255 109 N C 0.271 175.458 175.510 -0.539 0.000 1.008 109 N CA 1.607 54.022 53.050 -1.058 0.000 0.742 109 N CB -0.963 36.387 38.487 -1.894 0.000 0.906 109 N HN 0.895 nan 8.380 nan 0.000 0.541 110 N N -1.857 116.641 118.700 -0.338 0.000 2.863 110 N HA -0.233 4.507 4.740 0.000 0.000 0.245 110 N C -0.391 175.023 175.510 -0.159 0.000 1.001 110 N CA 1.190 54.115 53.050 -0.208 0.000 0.901 110 N CB -0.764 37.609 38.487 -0.188 0.000 1.124 110 N HN 0.520 nan 8.380 nan 0.000 0.582 111 S N -2.008 113.590 115.700 -0.171 0.000 3.287 111 S HA 0.725 5.195 4.470 0.000 0.000 0.324 111 S C -0.715 173.832 174.600 -0.089 0.000 1.205 111 S CA 0.131 58.269 58.200 -0.103 0.000 1.020 111 S CB 1.219 64.368 63.200 -0.086 0.000 1.398 111 S HN 0.279 nan 8.310 nan 0.000 0.679 112 T N -1.212 113.310 114.554 -0.053 0.000 2.858 112 T HA 0.622 4.972 4.350 0.000 0.000 0.285 112 T C -0.004 174.668 174.700 -0.046 0.000 1.052 112 T CA -0.480 61.599 62.100 -0.037 0.000 1.009 112 T CB 0.526 69.423 68.868 0.048 0.000 1.241 112 T HN 0.382 nan 8.240 nan 0.000 0.542 113 F N 0.918 120.959 119.950 0.153 0.000 2.797 113 F HA 0.460 4.987 4.527 0.000 0.000 0.302 113 F C 1.960 177.809 175.800 0.082 0.000 1.130 113 F CA -0.224 57.852 58.000 0.127 0.000 1.387 113 F CB -0.294 38.749 39.000 0.072 0.000 1.107 113 F HN 0.781 nan 8.300 nan 0.000 0.577 114 A N 0.000 122.944 122.820 0.207 0.000 2.254 114 A HA 0.000 4.320 4.320 0.000 0.000 0.244 114 A CA 0.000 52.120 52.037 0.138 0.000 0.836 114 A CB 0.000 19.057 19.000 0.096 0.000 0.831 114 A HN 0.000 nan 8.150 nan 0.000 0.486