REMARK SPARTA+ Protein Chemical Shift Prediction Table REMARK REMARK All chemical shifts are reported in ppm: REMARK REMARK SS_SHIFT is the predicted secondary chemical shift. REMARK SHIFT is the predicted chemical shift. REMARK RC_SHIFT is the random coil chemical shift. REMARK HM_SHIFT is the ring current shift; a correction REMARK of 0.6 times this value is applied. REMARK EF_SHIFT is the adjustment for electric field effect, REMARK applied to proton shifts only. REMARK SIGMA is the estimated prediction error. REMARK REMARK Reference: REMARK Y. Shen and A. Bax: SPARTA+: a modest improvement in empirical NMR REMARK chemical shift prediction by means of an artificial neural network. REMARK J. Biomol. NMR 48, 13-22 (2010). DATA SOURCE 1ovo_1_A DATA FIRST_RESID 1 DATA SEQUENCE LAAVSVDcSE YPKPAcPKDY RPVcGSDNKT YSNKcNFcNA VVESNGTLTL DATA SEQUENCE NHFGKc VARS RESID RESNAME ATOMNAME SS_SHIFT SHIFT RC_SHIFT HM_SHIFT EF_SHIFT SIGMA FORMAT %4d %4s %4s %9.3f %9.3f %9.3f %9.3f %9.3f %9.3f 1 L HA 0.000 nan 4.340 nan 0.000 0.249 1 L C 0.000 176.879 176.870 0.015 0.000 1.165 1 L CA 0.000 54.847 54.840 0.012 0.000 0.813 1 L CB 0.000 42.066 42.059 0.012 0.000 0.961 2 A N 3.123 125.953 122.820 0.016 0.000 2.318 2 A HA 1.045 5.365 4.320 -0.000 0.000 0.324 2 A C -0.632 176.968 177.584 0.025 0.000 1.170 2 A CA 0.278 52.326 52.037 0.019 0.000 0.810 2 A CB 1.451 20.460 19.000 0.015 0.000 1.198 2 A HN 1.394 nan 8.150 nan 0.000 0.484 3 A N 1.310 124.150 122.820 0.033 0.000 2.532 3 A HA 0.791 5.111 4.320 -0.000 0.000 0.290 3 A C -1.244 176.378 177.584 0.062 0.000 1.143 3 A CA -0.531 51.533 52.037 0.046 0.000 0.728 3 A CB 1.331 20.359 19.000 0.047 0.000 1.317 3 A HN 1.280 nan 8.150 nan 0.000 0.414 4 V N 1.566 121.537 119.914 0.095 0.000 2.417 4 V HA 0.688 4.808 4.120 -0.000 0.000 0.291 4 V C 0.150 176.382 176.094 0.231 0.000 1.024 4 V CA 0.482 62.873 62.300 0.151 0.000 0.861 4 V CB 1.506 33.438 31.823 0.181 0.000 0.985 4 V HN 1.361 nan 8.190 nan 0.000 0.436 5 S N 5.105 120.950 115.700 0.241 0.000 2.661 5 S HA 0.871 5.341 4.470 -0.000 0.000 0.285 5 S C -1.150 173.646 174.600 0.326 0.000 1.138 5 S CA -0.741 57.593 58.200 0.225 0.000 0.855 5 S CB 2.173 65.446 63.200 0.122 0.000 1.136 5 S HN 0.533 nan 8.310 nan 0.000 0.484 6 V N 1.403 121.463 119.914 0.243 0.000 2.540 6 V HA 0.537 4.657 4.120 -0.000 0.000 0.302 6 V C -0.989 175.195 176.094 0.151 0.000 1.035 6 V CA -0.721 61.720 62.300 0.235 0.000 0.873 6 V CB 1.640 33.607 31.823 0.239 0.000 0.992 6 V HN 0.981 nan 8.190 nan 0.000 0.428 7 D N 2.394 122.877 120.400 0.138 0.000 2.359 7 D HA 0.291 4.931 4.640 -0.000 0.000 0.230 7 D C 0.139 176.528 176.300 0.148 0.000 1.118 7 D CA -0.199 53.872 54.000 0.118 0.000 0.844 7 D CB 1.598 42.462 40.800 0.106 0.000 1.059 7 D HN 0.655 nan 8.370 nan 0.000 0.493 8 c N 2.678 121.348 118.600 0.117 0.000 2.688 8 c HA 0.130 4.700 4.570 -0.000 0.000 0.297 8 c C 2.262 176.492 174.090 0.235 0.000 1.308 8 c CA -0.313 56.108 56.329 0.154 0.000 1.726 8 c CB -1.272 41.224 42.510 -0.024 0.000 1.982 8 c HN 0.589 nan 8.230 nan 0.000 0.604 9 S N 1.335 117.133 115.700 0.162 0.000 2.382 9 S HA -0.165 4.305 4.470 -0.000 0.000 0.228 9 S C 1.561 176.191 174.600 0.050 0.000 1.027 9 S CA 1.365 59.618 58.200 0.088 0.000 0.991 9 S CB -0.165 63.066 63.200 0.052 0.000 0.823 9 S HN 0.727 nan 8.310 nan 0.000 0.469 10 E N -0.520 119.689 120.200 0.015 0.000 2.511 10 E HA 0.049 4.399 4.350 -0.000 0.000 0.196 10 E C -0.627 175.705 176.600 -0.447 0.000 1.066 10 E CA 0.174 56.435 56.400 -0.232 0.000 0.871 10 E CB 0.152 29.646 29.700 -0.343 0.000 0.863 10 E HN 0.526 nan 8.360 nan 0.000 0.520 11 Y N 0.391 120.682 120.300 -0.016 0.000 2.534 11 Y HA 0.298 4.848 4.550 -0.000 0.000 0.329 11 Y C -1.851 174.029 175.900 -0.033 0.000 1.154 11 Y CA -2.922 55.162 58.100 -0.026 0.000 1.192 11 Y CB 0.350 38.785 38.460 -0.041 0.000 1.275 11 Y HN -0.087 nan 8.280 nan 0.000 0.491 12 P HA 0.251 nan 4.420 nan 0.000 0.274 12 P C -1.500 175.838 177.300 0.063 0.000 1.237 12 P CA -0.630 62.559 63.100 0.149 0.000 0.793 12 P CB 0.760 32.506 31.700 0.077 0.000 0.977 13 K N 1.783 122.258 120.400 0.126 0.000 2.426 13 K HA 0.292 4.612 4.320 -0.000 0.000 0.254 13 K C -1.857 174.785 176.600 0.069 0.000 0.936 13 K CA -1.633 54.681 56.287 0.045 0.000 0.801 13 K CB 1.843 34.359 32.500 0.026 0.000 1.139 13 K HN 0.215 nan 8.250 nan 0.000 0.424 14 P HA -0.128 nan 4.420 nan 0.000 0.222 14 P C -0.347 176.980 177.300 0.045 0.000 1.147 14 P CA 0.804 63.926 63.100 0.036 0.000 0.790 14 P CB 0.346 32.058 31.700 0.020 0.000 0.780 15 A N -1.382 121.468 122.820 0.049 0.000 2.386 15 A HA 0.598 4.917 4.320 -0.000 0.000 0.311 15 A C -0.733 176.899 177.584 0.079 0.000 1.068 15 A CA -0.424 51.647 52.037 0.055 0.000 0.743 15 A CB 1.082 20.106 19.000 0.040 0.000 1.258 15 A HN 0.087 nan 8.150 nan 0.000 0.429 16 c N 1.819 120.471 118.600 0.085 0.000 2.667 16 c HA 0.804 5.374 4.570 -0.000 0.000 0.323 16 c C -2.103 172.039 174.090 0.088 0.000 1.214 16 c CA -0.834 55.558 56.329 0.106 0.000 1.721 16 c CB 1.184 43.764 42.510 0.117 0.000 2.275 16 c HN 0.827 nan 8.230 nan 0.000 0.491 17 P HA 0.241 nan 4.420 nan 0.000 0.272 17 P C -0.200 177.151 177.300 0.085 0.000 1.230 17 P CA 0.001 63.150 63.100 0.080 0.000 0.788 17 P CB 0.498 32.247 31.700 0.082 0.000 0.949 18 K N -0.124 120.321 120.400 0.076 0.000 2.387 18 K HA 0.102 4.422 4.320 -0.000 0.000 0.198 18 K C -0.352 176.309 176.600 0.101 0.000 1.022 18 K CA -0.166 56.169 56.287 0.081 0.000 1.128 18 K CB 0.063 32.598 32.500 0.059 0.000 0.853 18 K HN 0.445 nan 8.250 nan 0.000 0.523 19 D N 1.337 121.797 120.400 0.099 0.000 2.531 19 D HA -0.137 4.503 4.640 -0.000 0.000 0.239 19 D C -0.801 175.597 176.300 0.164 0.000 1.144 19 D CA 0.555 54.622 54.000 0.113 0.000 0.869 19 D CB 0.170 41.019 40.800 0.081 0.000 1.160 19 D HN 0.132 nan 8.370 nan 0.000 0.484 20 Y N 3.041 123.362 120.300 0.035 0.000 2.518 20 Y HA 0.278 4.828 4.550 -0.000 0.000 0.344 20 Y C 0.071 175.993 175.900 0.038 0.000 0.982 20 Y CA -0.641 57.481 58.100 0.037 0.000 1.234 20 Y CB 0.330 38.807 38.460 0.028 0.000 1.114 20 Y HN 0.209 nan 8.280 nan 0.000 0.515 21 R N 6.980 127.334 120.500 -0.243 0.000 2.644 21 R HA 0.246 4.586 4.340 -0.000 0.000 0.271 21 R C -2.860 173.286 176.300 -0.256 0.000 1.687 21 R CA -1.873 54.084 56.100 -0.238 0.000 1.655 21 R CB 0.573 30.828 30.300 -0.075 0.000 1.285 21 R HN 0.463 nan 8.270 nan 0.000 0.643 22 P HA -0.004 nan 4.420 nan 0.000 0.266 22 P C -0.341 176.885 177.300 -0.124 0.000 1.195 22 P CA 0.015 62.931 63.100 -0.307 0.000 0.768 22 P CB 0.983 32.467 31.700 -0.359 0.000 0.838 23 V N 2.916 122.760 119.914 -0.116 0.000 2.588 23 V HA 0.232 4.352 4.120 -0.000 0.000 0.304 23 V C 0.063 175.988 176.094 -0.283 0.000 1.042 23 V CA -0.614 61.517 62.300 -0.282 0.000 0.877 23 V CB 2.163 33.655 31.823 -0.552 0.000 0.996 23 V HN 0.735 nan 8.190 nan 0.000 0.425 24 c N 4.888 123.223 118.600 -0.442 0.000 2.303 24 c HA 0.779 5.349 4.570 -0.000 0.000 0.341 24 c C 1.063 174.941 174.090 -0.353 0.000 1.244 24 c CA -0.143 55.942 56.329 -0.406 0.000 1.765 24 c CB -0.771 41.249 42.510 -0.817 0.000 2.379 24 c HN 1.086 nan 8.230 nan 0.000 0.530 25 G N 3.576 112.371 108.800 -0.008 0.000 2.528 25 G HA2 0.385 4.345 3.960 -0.000 0.000 0.289 25 G HA3 0.385 4.345 3.960 -0.000 0.000 0.289 25 G C 0.847 175.783 174.900 0.060 0.000 1.192 25 G CA 0.257 45.447 45.100 0.150 0.000 0.921 25 G HN 1.176 nan 8.290 nan 0.000 0.512 26 S N -0.991 114.755 115.700 0.076 0.000 2.607 26 S HA -0.076 4.394 4.470 -0.000 0.000 0.224 26 S C 1.155 175.782 174.600 0.044 0.000 0.969 26 S CA 1.028 59.247 58.200 0.032 0.000 0.927 26 S CB 0.011 63.227 63.200 0.026 0.000 0.772 26 S HN 0.632 nan 8.310 nan 0.000 0.533 27 D N 0.885 121.333 120.400 0.081 0.000 2.319 27 D HA 0.032 4.672 4.640 -0.000 0.000 0.230 27 D C -0.036 176.291 176.300 0.046 0.000 1.094 27 D CA -0.265 53.777 54.000 0.069 0.000 0.856 27 D CB -1.609 39.253 40.800 0.104 0.000 0.915 27 D HN 0.453 nan 8.370 nan 0.000 0.517 28 N N -0.185 118.534 118.700 0.032 0.000 2.710 28 N HA -0.213 4.527 4.740 -0.000 0.000 0.249 28 N C -0.903 174.600 175.510 -0.010 0.000 1.059 28 N CA 0.771 53.823 53.050 0.003 0.000 0.720 28 N CB -0.805 37.686 38.487 0.006 0.000 0.983 28 N HN 0.408 nan 8.380 nan 0.000 0.544 29 K N -0.034 120.364 120.400 -0.003 0.000 2.203 29 K HA 0.469 4.789 4.320 -0.000 0.000 0.251 29 K C 0.045 176.559 176.600 -0.144 0.000 0.944 29 K CA -0.697 55.529 56.287 -0.102 0.000 0.829 29 K CB 1.917 34.303 32.500 -0.190 0.000 1.125 29 K HN 0.045 nan 8.250 nan 0.000 0.430 30 T N 1.827 116.270 114.554 -0.185 0.000 2.743 30 T HA 0.321 4.671 4.350 -0.000 0.000 0.292 30 T C -0.980 173.604 174.700 -0.194 0.000 0.972 30 T CA -0.465 61.562 62.100 -0.122 0.000 0.967 30 T CB -0.042 68.801 68.868 -0.041 0.000 0.926 30 T HN 0.290 nan 8.240 nan 0.000 0.459 31 Y N 3.242 123.544 120.300 0.004 0.000 2.313 31 Y HA 0.266 4.816 4.550 -0.000 0.000 0.332 31 Y C 2.029 177.947 175.900 0.031 0.000 1.071 31 Y CA -0.382 57.749 58.100 0.052 0.000 1.169 31 Y CB 1.486 40.023 38.460 0.127 0.000 1.192 31 Y HN 0.850 nan 8.280 nan 0.000 0.487 32 S N 1.162 116.981 115.700 0.198 0.000 2.423 32 S HA -0.089 4.381 4.470 -0.000 0.000 0.231 32 S C 0.166 174.823 174.600 0.096 0.000 1.014 32 S CA 1.190 59.508 58.200 0.196 0.000 0.965 32 S CB -0.604 62.752 63.200 0.259 0.000 0.785 32 S HN 0.868 nan 8.310 nan 0.000 0.495 33 N N -0.478 118.296 118.700 0.123 0.000 3.179 33 N HA 0.252 4.992 4.740 -0.000 0.000 0.250 33 N C -0.058 175.511 175.510 0.097 0.000 1.507 33 N CA -0.851 52.244 53.050 0.076 0.000 0.883 33 N CB 0.751 39.288 38.487 0.082 0.000 1.435 33 N HN -0.076 nan 8.380 nan 0.000 0.532 34 K N -0.579 119.861 120.400 0.067 0.000 2.103 34 K HA -0.158 4.162 4.320 -0.000 0.000 0.207 34 K C 1.438 177.978 176.600 -0.101 0.000 1.048 34 K CA 1.758 58.072 56.287 0.045 0.000 0.930 34 K CB -0.438 32.092 32.500 0.049 0.000 0.716 34 K HN 0.593 nan 8.250 nan 0.000 0.444 35 c N 1.274 119.843 118.600 -0.051 0.000 2.446 35 c HA -0.034 4.536 4.570 -0.000 0.000 0.277 35 c C 2.121 176.173 174.090 -0.064 0.000 1.275 35 c CA 1.384 57.663 56.329 -0.082 0.000 1.727 35 c CB -1.314 41.309 42.510 0.188 0.000 2.010 35 c HN 0.587 nan 8.230 nan 0.000 0.486 36 N N -0.532 118.198 118.700 0.051 0.000 2.188 36 N HA -0.067 4.673 4.740 -0.000 0.000 0.184 36 N C 1.557 177.077 175.510 0.017 0.000 1.018 36 N CA 1.593 54.711 53.050 0.115 0.000 0.858 36 N CB -0.398 38.241 38.487 0.252 0.000 0.989 36 N HN 0.682 nan 8.380 nan 0.000 0.426 37 F N 0.941 120.764 119.950 -0.212 0.000 2.102 37 F HA -0.221 4.306 4.527 0.000 0.000 0.298 37 F C 1.989 177.487 175.800 -0.503 0.000 1.105 37 F CA 1.115 58.773 58.000 -0.571 0.000 1.239 37 F CB -0.263 38.498 39.000 -0.398 0.000 0.991 37 F HN 0.054 nan 8.300 nan 0.000 0.474 38 c N 0.729 118.976 118.600 -0.588 0.000 2.440 38 c HA -0.133 4.437 4.570 -0.000 0.000 0.278 38 c C 2.458 176.293 174.090 -0.425 0.000 1.295 38 c CA 1.426 57.352 56.329 -0.673 0.000 1.738 38 c CB -1.849 40.094 42.510 -0.946 0.000 1.987 38 c HN 0.577 nan 8.230 nan 0.000 0.492 39 N N 1.005 119.543 118.700 -0.270 0.000 2.104 39 N HA -0.139 4.601 4.740 -0.000 0.000 0.190 39 N C 1.864 177.286 175.510 -0.147 0.000 1.024 39 N CA 1.394 54.392 53.050 -0.087 0.000 0.853 39 N CB -0.173 38.314 38.487 -0.000 0.000 1.008 39 N HN 0.531 nan 8.380 nan 0.000 0.424 40 A N 0.661 123.338 122.820 -0.239 0.000 1.898 40 A HA -0.066 4.254 4.320 -0.000 0.000 0.216 40 A C 2.391 179.774 177.584 -0.335 0.000 1.181 40 A CA 0.972 52.863 52.037 -0.243 0.000 0.620 40 A CB -0.705 18.127 19.000 -0.279 0.000 0.819 40 A HN 0.091 nan 8.150 nan 0.000 0.442 41 V N -0.209 119.369 119.914 -0.560 0.000 2.255 41 V HA -0.264 3.856 4.120 -0.000 0.000 0.247 41 V C 2.569 178.505 176.094 -0.262 0.000 1.051 41 V CA 2.217 64.218 62.300 -0.498 0.000 1.018 41 V CB -0.863 30.541 31.823 -0.699 0.000 0.641 41 V HN 0.377 nan 8.190 nan 0.000 0.445 42 V N -0.292 119.499 119.914 -0.205 0.000 2.515 42 V HA -0.222 3.898 4.120 -0.000 0.000 0.250 42 V C 2.280 178.326 176.094 -0.079 0.000 1.058 42 V CA 1.875 64.111 62.300 -0.106 0.000 1.064 42 V CB -0.677 31.116 31.823 -0.051 0.000 0.675 42 V HN 0.626 nan 8.190 nan 0.000 0.461 43 E N 0.515 120.666 120.200 -0.083 0.000 2.358 43 E HA -0.095 4.255 4.350 -0.000 0.000 0.195 43 E C 2.151 178.719 176.600 -0.054 0.000 1.010 43 E CA 1.041 57.410 56.400 -0.053 0.000 0.856 43 E CB -0.091 29.586 29.700 -0.039 0.000 0.795 43 E HN 0.721 nan 8.360 nan 0.000 0.504 44 S N 0.477 116.128 115.700 -0.081 0.000 2.607 44 S HA -0.078 4.392 4.470 -0.000 0.000 0.224 44 S C 0.450 175.020 174.600 -0.051 0.000 0.969 44 S CA -0.112 58.049 58.200 -0.065 0.000 0.927 44 S CB -0.554 62.592 63.200 -0.089 0.000 0.772 44 S HN 0.225 nan 8.310 nan 0.000 0.533 45 N N 1.639 120.308 118.700 -0.052 0.000 2.722 45 N HA -0.207 4.533 4.740 -0.000 0.000 0.274 45 N C 0.796 176.283 175.510 -0.039 0.000 0.987 45 N CA 0.242 53.267 53.050 -0.042 0.000 0.817 45 N CB -1.247 37.223 38.487 -0.028 0.000 0.921 45 N HN 0.851 nan 8.380 nan 0.000 0.565 46 G N -1.163 107.607 108.800 -0.051 0.000 2.259 46 G HA2 -0.330 3.630 3.960 -0.000 0.000 0.217 46 G HA3 -0.330 3.630 3.960 -0.000 0.000 0.217 46 G C 0.824 175.701 174.900 -0.039 0.000 1.001 46 G CA 0.639 45.714 45.100 -0.041 0.000 0.627 46 G HN 0.626 nan 8.290 nan 0.000 0.501 47 T N -1.588 112.942 114.554 -0.040 0.000 3.065 47 T HA 0.564 4.914 4.350 -0.000 0.000 0.252 47 T C 0.798 175.480 174.700 -0.030 0.000 1.099 47 T CA 1.015 63.098 62.100 -0.027 0.000 1.063 47 T CB 0.528 69.385 68.868 -0.018 0.000 0.948 47 T HN 0.974 nan 8.240 nan 0.000 0.506 48 L N 1.545 122.732 121.223 -0.060 0.000 2.307 48 L HA 0.748 5.088 4.340 -0.000 0.000 0.284 48 L C -0.246 176.575 176.870 -0.082 0.000 1.023 48 L CA -0.009 54.791 54.840 -0.067 0.000 0.810 48 L CB 1.978 43.962 42.059 -0.126 0.000 1.231 48 L HN 0.151 nan 8.230 nan 0.000 0.423 49 T N 3.328 117.882 114.554 -0.001 0.000 2.693 49 T HA 0.509 4.859 4.350 -0.000 0.000 0.278 49 T C -1.349 173.465 174.700 0.190 0.000 0.994 49 T CA -0.620 61.510 62.100 0.050 0.000 1.033 49 T CB 0.885 69.763 68.868 0.018 0.000 1.342 49 T HN 0.564 nan 8.240 nan 0.000 0.538 50 L N 2.922 124.237 121.223 0.153 0.000 2.331 50 L HA 0.470 4.810 4.340 -0.000 0.000 0.278 50 L C 0.907 177.695 176.870 -0.137 0.000 1.106 50 L CA 0.476 55.274 54.840 -0.069 0.000 0.824 50 L CB 0.353 42.294 42.059 -0.195 0.000 1.142 50 L HN 0.801 nan 8.230 nan 0.000 0.443 51 N N 1.427 119.986 118.700 -0.235 0.000 2.414 51 N HA 0.110 4.850 4.740 -0.000 0.000 0.189 51 N C -0.819 174.601 175.510 -0.149 0.000 1.039 51 N CA 0.513 53.459 53.050 -0.174 0.000 0.889 51 N CB 0.249 38.600 38.487 -0.225 0.000 1.085 51 N HN 0.877 nan 8.380 nan 0.000 0.442 52 H N -3.066 115.848 119.070 -0.260 0.000 2.967 52 H HA 0.291 4.847 4.556 -0.000 0.000 0.318 52 H C -1.593 173.502 175.328 -0.387 0.000 1.375 52 H CA -1.033 54.846 56.048 -0.281 0.000 1.132 52 H CB 0.044 29.742 29.762 -0.106 0.000 1.848 52 H HN -0.151 nan 8.280 nan 0.000 0.524 53 F N 1.139 121.125 119.950 0.061 0.000 2.418 53 F HA 0.501 5.028 4.527 -0.000 0.000 0.341 53 F C 1.548 177.414 175.800 0.110 0.000 1.120 53 F CA 1.727 59.713 58.000 -0.022 0.000 1.232 53 F CB 1.058 40.032 39.000 -0.043 0.000 1.175 53 F HN 1.148 nan 8.300 nan 0.000 0.569 54 G N 2.035 110.943 108.800 0.181 0.000 2.661 54 G HA2 -0.167 3.793 3.960 -0.000 0.000 0.685 54 G HA3 -0.167 3.793 3.960 -0.000 0.000 0.685 54 G C -0.984 173.957 174.900 0.068 0.000 1.298 54 G CA -1.080 44.089 45.100 0.115 0.000 0.855 54 G HN 0.792 nan 8.290 nan 0.000 0.560 55 K N -0.477 119.962 120.400 0.065 0.000 2.436 55 K HA 0.382 4.701 4.320 -0.000 0.000 0.275 55 K C 1.185 177.869 176.600 0.140 0.000 0.999 55 K CA -0.093 56.233 56.287 0.065 0.000 0.980 55 K CB -0.052 32.501 32.500 0.088 0.000 0.919 55 K HN 0.679 nan 8.250 nan 0.000 0.484 56 c N 0.000 118.629 118.600 0.048 0.000 0.000 56 c HA 0.000 4.570 4.570 -0.000 0.000 0.000 56 c CA 0.000 56.372 56.329 0.072 0.000 0.000 56 c CB 0.000 42.502 42.510 -0.013 0.000 0.000 56 c HN 0.000 nan 8.230 nan 0.000 0.000