REMARK SPARTA+ Protein Chemical Shift Prediction Table REMARK REMARK All chemical shifts are reported in ppm: REMARK REMARK SS_SHIFT is the predicted secondary chemical shift. REMARK SHIFT is the predicted chemical shift. REMARK RC_SHIFT is the random coil chemical shift. REMARK HM_SHIFT is the ring current shift; a correction REMARK of 0.6 times this value is applied. REMARK EF_SHIFT is the adjustment for electric field effect, REMARK applied to proton shifts only. REMARK SIGMA is the estimated prediction error. REMARK REMARK Reference: REMARK Y. Shen and A. Bax: SPARTA+: a modest improvement in empirical NMR REMARK chemical shift prediction by means of an artificial neural network. REMARK J. Biomol. NMR 48, 13-22 (2010). DATA SOURCE 1zc4_1_B DATA FIRST_RESID 171 DATA SEQUENCE GQYLVYNGDL VEYEADHMAQ LQRVHGFLMN DCLLVATWLP QRRGMYRYNA DATA SEQUENCE LYPLDRLAVV NVKDNPPMKD MFKLLMFPES RIFQAENAKI KREWLEVLEE DATA SEQUENCE TKRALSDKRR REQ VARS RESID RESNAME ATOMNAME SS_SHIFT SHIFT RC_SHIFT HM_SHIFT EF_SHIFT SIGMA FORMAT %4d %4s %4s %9.3f %9.3f %9.3f %9.3f %9.3f %9.3f 171 G HA2 0.000 nan 3.960 nan 0.000 0.244 171 G HA3 0.000 3.960 3.960 -0.001 0.000 0.244 171 G C 0.000 174.980 174.900 0.133 0.000 0.946 171 G CA 0.000 45.125 45.100 0.042 0.000 0.502 172 Q N 0.903 120.785 119.800 0.136 0.000 2.304 172 Q HA 0.657 4.996 4.340 -0.001 0.000 0.260 172 Q C -0.686 175.479 176.000 0.276 0.000 0.965 172 Q CA -0.176 55.736 55.803 0.182 0.000 0.898 172 Q CB 0.594 29.389 28.738 0.094 0.000 1.196 172 Q HN 0.981 nan 8.270 nan 0.000 0.402 173 Y N 1.521 121.835 120.300 0.024 0.000 2.689 173 Y HA 0.522 5.072 4.550 -0.001 0.000 0.333 173 Y C -2.133 173.792 175.900 0.042 0.000 1.208 173 Y CA -1.826 56.289 58.100 0.026 0.000 1.055 173 Y CB 0.380 38.852 38.460 0.020 0.000 1.304 173 Y HN 0.476 nan 8.280 nan 0.000 0.455 174 L N 2.761 123.942 121.223 -0.070 0.000 2.295 174 L HA 0.416 4.756 4.340 -0.001 0.000 0.288 174 L C 0.137 176.858 176.870 -0.248 0.000 1.079 174 L CA 0.018 54.785 54.840 -0.123 0.000 0.830 174 L CB 0.576 42.657 42.059 0.037 0.000 1.200 174 L HN 0.812 nan 8.230 nan 0.000 0.438 175 V N 5.688 125.361 119.914 -0.401 0.000 2.488 175 V HA -0.041 4.079 4.120 -0.001 0.000 0.246 175 V C 0.137 176.222 176.094 -0.015 0.000 1.046 175 V CA 0.968 63.092 62.300 -0.293 0.000 1.053 175 V CB -0.718 30.933 31.823 -0.287 0.000 0.679 175 V HN 0.784 nan 8.190 nan 0.000 0.458 176 Y N 0.431 120.619 120.300 -0.186 0.000 2.662 176 Y HA 0.473 5.023 4.550 -0.000 0.000 0.334 176 Y C -1.327 174.422 175.900 -0.250 0.000 1.185 176 Y CA -1.461 56.474 58.100 -0.274 0.000 1.074 176 Y CB 1.323 39.508 38.460 -0.459 0.000 1.330 176 Y HN 0.275 nan 8.280 nan 0.000 0.458 177 N N 2.079 120.413 118.700 -0.609 0.000 2.961 177 N HA 0.732 5.471 4.740 -0.001 0.000 0.245 177 N C -1.292 173.928 175.510 -0.484 0.000 1.404 177 N CA -0.269 52.575 53.050 -0.344 0.000 0.880 177 N CB 1.981 40.395 38.487 -0.121 0.000 1.461 177 N HN 1.212 nan 8.380 nan 0.000 0.510 178 G N 0.393 108.927 108.800 -0.443 0.000 2.325 178 G HA2 0.290 4.250 3.960 -0.001 0.000 0.297 178 G HA3 0.290 4.250 3.960 -0.001 0.000 0.297 178 G C -2.211 172.421 174.900 -0.446 0.000 1.448 178 G CA -0.806 44.107 45.100 -0.312 0.000 0.838 178 G HN 0.598 nan 8.290 nan 0.000 0.579 179 D N -0.579 119.762 120.400 -0.099 0.000 2.313 179 D HA 0.728 5.367 4.640 -0.001 0.000 0.247 179 D C 0.390 176.732 176.300 0.070 0.000 1.094 179 D CA 0.226 54.247 54.000 0.036 0.000 0.925 179 D CB 1.505 42.433 40.800 0.214 0.000 1.188 179 D HN 0.401 nan 8.370 nan 0.000 0.430 180 L N 0.204 121.435 121.223 0.013 0.000 2.491 180 L HA 0.550 4.890 4.340 -0.001 0.000 0.254 180 L C -1.302 175.589 176.870 0.036 0.000 1.048 180 L CA -1.212 53.636 54.840 0.013 0.000 0.855 180 L CB 2.023 43.994 42.059 -0.146 0.000 1.466 180 L HN 0.086 nan 8.230 nan 0.000 0.409 181 V N 0.628 120.599 119.914 0.096 0.000 2.409 181 V HA 0.299 4.418 4.120 -0.001 0.000 0.291 181 V C -0.146 176.035 176.094 0.145 0.000 1.020 181 V CA -0.546 61.797 62.300 0.073 0.000 0.848 181 V CB 1.480 33.367 31.823 0.108 0.000 0.990 181 V HN 0.715 nan 8.190 nan 0.000 0.430 182 E N 3.410 123.624 120.200 0.024 0.000 2.373 182 E HA 0.434 4.784 4.350 -0.001 0.000 0.263 182 E C -1.673 174.820 176.600 -0.179 0.000 1.073 182 E CA -0.272 56.072 56.400 -0.093 0.000 0.894 182 E CB 0.886 30.512 29.700 -0.123 0.000 1.008 182 E HN 0.584 nan 8.360 nan 0.000 0.420 183 Y N 0.789 120.907 120.300 -0.303 0.000 2.553 183 Y HA 0.191 4.741 4.550 -0.000 0.000 0.347 183 Y C -0.185 175.587 175.900 -0.214 0.000 1.019 183 Y CA -0.972 57.004 58.100 -0.207 0.000 1.032 183 Y CB 1.695 40.048 38.460 -0.178 0.000 1.284 183 Y HN 0.443 nan 8.280 nan 0.000 0.466 184 E N 1.660 121.863 120.200 0.004 0.000 2.223 184 E HA 0.306 4.656 4.350 -0.001 0.000 0.282 184 E C 0.472 177.066 176.600 -0.010 0.000 1.046 184 E CA 0.303 56.690 56.400 -0.020 0.000 0.857 184 E CB 1.595 31.302 29.700 0.012 0.000 1.055 184 E HN 0.901 nan 8.360 nan 0.000 0.409 185 A N 5.646 128.425 122.820 -0.068 0.000 1.869 185 A HA -0.268 4.051 4.320 -0.001 0.000 0.218 185 A C 1.539 179.068 177.584 -0.091 0.000 1.203 185 A CA 2.161 54.144 52.037 -0.090 0.000 0.638 185 A CB -0.378 18.557 19.000 -0.108 0.000 0.831 185 A HN 0.680 nan 8.150 nan 0.000 0.450 186 D N -1.126 119.205 120.400 -0.116 0.000 2.097 186 D HA -0.087 4.553 4.640 -0.001 0.000 0.197 186 D C 1.494 177.597 176.300 -0.327 0.000 0.984 186 D CA 1.729 55.582 54.000 -0.244 0.000 0.826 186 D CB -0.460 40.136 40.800 -0.339 0.000 0.973 186 D HN 0.748 nan 8.370 nan 0.000 0.460 187 H N -1.330 117.727 119.070 -0.022 0.000 2.551 187 H HA 0.300 4.856 4.556 -0.000 0.000 0.271 187 H C 0.425 175.754 175.328 0.002 0.000 0.984 187 H CA -0.079 55.961 56.048 -0.013 0.000 1.164 187 H CB 0.382 30.135 29.762 -0.014 0.000 1.437 187 H HN -0.169 nan 8.280 nan 0.000 0.550 188 M N 0.435 120.086 119.600 0.086 0.000 2.302 188 M HA -0.205 4.275 4.480 -0.001 0.000 0.200 188 M C -0.512 175.912 176.300 0.206 0.000 0.366 188 M CA 0.428 55.793 55.300 0.107 0.000 0.440 188 M CB -1.234 31.383 32.600 0.029 0.000 1.475 188 M HN 0.318 nan 8.290 nan 0.000 0.905 189 A N 1.094 124.003 122.820 0.149 0.000 2.301 189 A HA 0.552 4.871 4.320 -0.001 0.000 0.298 189 A C 0.264 177.802 177.584 -0.076 0.000 1.185 189 A CA -0.366 51.709 52.037 0.064 0.000 0.830 189 A CB 0.524 19.529 19.000 0.007 0.000 1.112 189 A HN 0.595 nan 8.150 nan 0.000 0.508 190 Q N 1.961 121.604 119.800 -0.262 0.000 2.315 190 Q HA 0.292 4.632 4.340 -0.001 0.000 0.289 190 Q C -0.449 175.276 176.000 -0.459 0.000 1.044 190 Q CA 0.321 55.653 55.803 -0.784 0.000 0.920 190 Q CB 0.357 28.720 28.738 -0.625 0.000 1.214 190 Q HN 0.773 nan 8.270 nan 0.000 0.392 191 L N 1.471 122.398 121.223 -0.492 0.000 2.644 191 L HA 0.319 4.659 4.340 -0.001 0.000 0.213 191 L C 0.162 176.877 176.870 -0.259 0.000 1.622 191 L CA -0.791 53.884 54.840 -0.276 0.000 2.969 191 L CB 0.065 42.000 42.059 -0.206 0.000 2.760 191 L HN 0.712 nan 8.230 nan 0.000 0.871 192 Q N 0.585 120.249 119.800 -0.227 0.000 2.633 192 Q HA 0.435 4.775 4.340 -0.001 0.000 0.292 192 Q C -1.107 174.711 176.000 -0.302 0.000 1.089 192 Q CA -0.875 54.775 55.803 -0.255 0.000 0.811 192 Q CB 1.483 30.075 28.738 -0.244 0.000 1.472 192 Q HN 0.242 nan 8.270 nan 0.000 0.464 193 R N -0.104 120.122 120.500 -0.457 0.000 2.490 193 R HA 0.590 4.930 4.340 -0.001 0.000 0.278 193 R C 0.184 176.054 176.300 -0.718 0.000 1.069 193 R CA -0.258 55.534 56.100 -0.512 0.000 1.080 193 R CB 1.057 30.995 30.300 -0.602 0.000 1.030 193 R HN 0.580 nan 8.270 nan 0.000 0.491 194 V N -1.146 118.580 119.914 -0.314 0.000 3.181 194 V HA 0.523 4.643 4.120 -0.001 0.000 0.308 194 V C -1.304 174.865 176.094 0.125 0.000 1.214 194 V CA -1.007 61.219 62.300 -0.123 0.000 1.053 194 V CB 2.141 33.946 31.823 -0.030 0.000 1.069 194 V HN 0.834 nan 8.190 nan 0.000 0.441 195 H N 0.078 119.238 119.070 0.151 0.000 2.529 195 H HA 0.797 5.352 4.556 -0.000 0.000 0.348 195 H C 0.106 175.325 175.328 -0.182 0.000 1.079 195 H CA 0.627 56.672 56.048 -0.005 0.000 1.198 195 H CB 1.842 31.606 29.762 0.003 0.000 1.521 195 H HN 1.289 nan 8.280 nan 0.000 0.514 196 G N 3.573 112.101 108.800 -0.454 0.000 2.379 196 G HA2 0.465 4.425 3.960 -0.001 0.000 0.327 196 G HA3 0.465 4.425 3.960 -0.001 0.000 0.327 196 G C -1.400 173.416 174.900 -0.139 0.000 1.145 196 G CA -0.481 44.514 45.100 -0.176 0.000 0.905 196 G HN 0.433 nan 8.290 nan 0.000 0.466 197 F N 1.664 121.758 119.950 0.240 0.000 2.460 197 F HA 0.364 4.891 4.527 -0.001 0.000 0.341 197 F C 0.118 176.025 175.800 0.178 0.000 1.130 197 F CA -0.768 57.352 58.000 0.201 0.000 0.962 197 F CB 2.236 41.322 39.000 0.143 0.000 1.171 197 F HN 0.152 nan 8.300 nan 0.000 0.436 198 L N 5.093 126.506 121.223 0.318 0.000 2.257 198 L HA 0.506 4.845 4.340 -0.001 0.000 0.290 198 L C -0.670 176.237 176.870 0.062 0.000 1.044 198 L CA -0.346 54.615 54.840 0.202 0.000 0.810 198 L CB 0.979 43.144 42.059 0.176 0.000 1.193 198 L HN 0.726 nan 8.230 nan 0.000 0.425 199 M N 2.827 122.449 119.600 0.035 0.000 2.679 199 M HA 0.240 4.720 4.480 -0.001 0.000 0.287 199 M C 1.172 177.417 176.300 -0.091 0.000 1.202 199 M CA -0.323 54.940 55.300 -0.061 0.000 0.911 199 M CB 0.981 33.576 32.600 -0.008 0.000 1.556 199 M HN 0.583 nan 8.290 nan 0.000 0.511 200 N N -0.164 118.471 118.700 -0.107 0.000 2.512 200 N HA -0.127 4.613 4.740 -0.001 0.000 0.183 200 N C -0.042 175.481 175.510 0.022 0.000 1.073 200 N CA 1.382 54.410 53.050 -0.038 0.000 0.911 200 N CB -0.295 38.233 38.487 0.068 0.000 0.964 200 N HN 0.662 nan 8.380 nan 0.000 0.447 201 D N -1.600 118.817 120.400 0.029 0.000 2.538 201 D HA 0.132 4.772 4.640 -0.001 0.000 0.241 201 D C -0.122 176.212 176.300 0.058 0.000 1.297 201 D CA -0.462 53.565 54.000 0.045 0.000 0.804 201 D CB -0.768 40.059 40.800 0.046 0.000 1.122 201 D HN 0.402 nan 8.370 nan 0.000 0.519 202 C N -1.009 118.330 119.300 0.064 0.000 3.307 202 C HA 0.804 5.264 4.460 -0.001 0.000 0.333 202 C C -1.685 173.379 174.990 0.124 0.000 1.291 202 C CA -1.261 57.814 59.018 0.094 0.000 1.273 202 C CB 0.637 28.422 27.740 0.075 0.000 1.580 202 C HN 0.213 nan 8.230 nan 0.000 0.481 203 L N 1.968 123.298 121.223 0.178 0.000 2.333 203 L HA 0.777 5.116 4.340 -0.001 0.000 0.280 203 L C -0.982 176.029 176.870 0.234 0.000 1.004 203 L CA -0.759 54.216 54.840 0.225 0.000 0.820 203 L CB 1.396 43.598 42.059 0.239 0.000 1.247 203 L HN 0.958 nan 8.230 nan 0.000 0.416 204 L N 6.101 127.469 121.223 0.241 0.000 2.296 204 L HA 0.637 4.976 4.340 -0.001 0.000 0.286 204 L C -1.247 175.775 176.870 0.254 0.000 1.023 204 L CA -0.689 54.279 54.840 0.213 0.000 0.812 204 L CB 1.718 43.913 42.059 0.226 0.000 1.223 204 L HN 0.474 nan 8.230 nan 0.000 0.421 205 V N 4.576 124.579 119.914 0.148 0.000 2.378 205 V HA 0.559 4.679 4.120 -0.001 0.000 0.288 205 V C 0.186 176.316 176.094 0.060 0.000 1.016 205 V CA -0.572 61.777 62.300 0.082 0.000 0.840 205 V CB 1.459 33.319 31.823 0.063 0.000 0.994 205 V HN 0.850 nan 8.190 nan 0.000 0.431 206 A N 3.752 126.682 122.820 0.183 0.000 2.288 206 A HA 0.827 5.147 4.320 -0.001 0.000 0.320 206 A C 0.089 177.844 177.584 0.284 0.000 1.217 206 A CA -0.390 51.781 52.037 0.224 0.000 0.840 206 A CB 0.865 20.097 19.000 0.386 0.000 1.179 206 A HN 0.714 nan 8.150 nan 0.000 0.504 207 T N 3.300 117.974 114.554 0.200 0.000 2.856 207 T HA 0.617 4.967 4.350 -0.001 0.000 0.283 207 T C -0.998 173.908 174.700 0.344 0.000 1.008 207 T CA -0.213 61.988 62.100 0.168 0.000 0.997 207 T CB 0.711 69.649 68.868 0.117 0.000 0.992 207 T HN 0.630 nan 8.240 nan 0.000 0.454 208 W N 3.321 124.646 121.300 0.042 0.000 2.391 208 W HA 0.543 5.203 4.660 -0.000 0.000 0.312 208 W C -1.599 174.947 176.519 0.045 0.000 1.003 208 W CA -1.767 55.591 57.345 0.022 0.000 1.375 208 W CB -0.693 28.781 29.460 0.024 0.000 1.253 208 W HN 0.352 nan 8.180 nan 0.000 0.416 209 L N 5.469 126.808 121.223 0.194 0.000 2.283 209 L HA 0.347 4.686 4.340 -0.001 0.000 0.281 209 L C -2.109 174.863 176.870 0.170 0.000 1.033 209 L CA -1.795 53.140 54.840 0.158 0.000 0.848 209 L CB 0.856 43.039 42.059 0.207 0.000 1.226 209 L HN -0.125 nan 8.230 nan 0.000 0.429 210 P HA 0.135 nan 4.420 nan 0.000 0.268 210 P C -0.834 176.527 177.300 0.102 0.000 1.205 210 P CA 0.022 63.195 63.100 0.122 0.000 0.771 210 P CB 0.711 32.473 31.700 0.103 0.000 0.858 211 Q N 1.219 121.077 119.800 0.097 0.000 2.456 211 Q HA 0.319 4.659 4.340 -0.001 0.000 0.283 211 Q C 0.864 176.897 176.000 0.054 0.000 1.084 211 Q CA -0.878 54.970 55.803 0.074 0.000 0.801 211 Q CB 2.035 30.826 28.738 0.088 0.000 1.434 211 Q HN 0.103 nan 8.270 nan 0.000 0.419 212 R N 0.664 121.186 120.500 0.037 0.000 2.143 212 R HA -0.117 4.222 4.340 -0.001 0.000 0.239 212 R C 0.315 176.633 176.300 0.030 0.000 1.126 212 R CA 2.019 58.136 56.100 0.028 0.000 0.927 212 R CB 0.216 30.526 30.300 0.017 0.000 0.860 212 R HN 0.301 nan 8.270 nan 0.000 0.433 213 R N -0.586 119.933 120.500 0.030 0.000 2.983 213 R HA 0.383 4.723 4.340 -0.001 0.000 0.300 213 R C -0.846 175.479 176.300 0.042 0.000 1.367 213 R CA 0.391 56.509 56.100 0.031 0.000 1.564 213 R CB 0.547 30.860 30.300 0.021 0.000 1.314 213 R HN 0.498 nan 8.270 nan 0.000 0.622 214 G N 1.672 110.506 108.800 0.057 0.000 2.496 214 G HA2 -0.113 3.847 3.960 -0.001 0.000 0.220 214 G HA3 -0.113 3.847 3.960 -0.001 0.000 0.220 214 G C -0.354 174.616 174.900 0.117 0.000 3.253 214 G CA -1.005 44.142 45.100 0.078 0.000 0.843 214 G HN 0.300 nan 8.290 nan 0.000 0.512 215 M N 2.558 122.221 119.600 0.105 0.000 2.603 215 M HA 0.270 4.750 4.480 -0.001 0.000 0.399 215 M C -0.447 175.952 176.300 0.164 0.000 1.745 215 M CA -0.125 55.251 55.300 0.125 0.000 1.055 215 M CB -0.319 32.337 32.600 0.093 0.000 2.144 215 M HN 0.487 nan 8.290 nan 0.000 0.483 216 Y N 7.041 127.391 120.300 0.083 0.000 2.383 216 Y HA 0.456 5.005 4.550 -0.001 0.000 0.344 216 Y C -0.182 175.802 175.900 0.140 0.000 0.986 216 Y CA -0.666 57.489 58.100 0.092 0.000 1.175 216 Y CB 0.431 38.931 38.460 0.068 0.000 1.152 216 Y HN 0.745 nan 8.280 nan 0.000 0.511 217 R N 5.785 125.911 120.500 -0.624 0.000 2.346 217 R HA 0.356 4.696 4.340 -0.001 0.000 0.311 217 R C -1.866 174.096 176.300 -0.562 0.000 0.983 217 R CA -0.777 55.095 56.100 -0.380 0.000 0.880 217 R CB 1.160 31.378 30.300 -0.137 0.000 1.100 217 R HN 0.801 nan 8.270 nan 0.000 0.453 218 Y N 3.734 123.846 120.300 -0.313 0.000 2.334 218 Y HA 0.383 4.932 4.550 -0.001 0.000 0.336 218 Y C -1.240 174.660 175.900 -0.001 0.000 0.960 218 Y CA -1.108 56.912 58.100 -0.133 0.000 1.164 218 Y CB 1.491 39.975 38.460 0.040 0.000 1.155 218 Y HN 0.667 nan 8.280 nan 0.000 0.478 219 N N 5.406 124.003 118.700 -0.172 0.000 2.407 219 N HA 0.558 5.297 4.740 -0.001 0.000 0.277 219 N C -1.384 173.922 175.510 -0.340 0.000 0.995 219 N CA -0.386 52.513 53.050 -0.252 0.000 0.903 219 N CB 2.016 40.455 38.487 -0.080 0.000 1.218 219 N HN 0.678 nan 8.380 nan 0.000 0.487 220 A N 2.606 125.159 122.820 -0.445 0.000 2.355 220 A HA 0.609 4.928 4.320 -0.001 0.000 0.317 220 A C -0.807 176.517 177.584 -0.434 0.000 1.094 220 A CA -0.674 51.095 52.037 -0.447 0.000 0.764 220 A CB 1.237 19.906 19.000 -0.551 0.000 1.230 220 A HN 0.623 nan 8.150 nan 0.000 0.448 221 L N 2.398 123.350 121.223 -0.451 0.000 2.313 221 L HA 0.544 4.884 4.340 -0.001 0.000 0.283 221 L C -1.678 174.935 176.870 -0.430 0.000 1.013 221 L CA -0.554 54.110 54.840 -0.294 0.000 0.816 221 L CB 1.303 43.285 42.059 -0.128 0.000 1.236 221 L HN 0.827 nan 8.230 nan 0.000 0.419 222 Y N 4.599 124.934 120.300 0.059 0.000 2.388 222 Y HA 0.350 4.899 4.550 -0.000 0.000 0.328 222 Y C -2.093 173.846 175.900 0.065 0.000 0.963 222 Y CA -2.510 55.632 58.100 0.068 0.000 1.240 222 Y CB 1.300 39.807 38.460 0.077 0.000 1.118 222 Y HN 0.483 nan 8.280 nan 0.000 0.484 223 P HA 0.063 nan 4.420 nan 0.000 0.267 223 P C 0.607 177.986 177.300 0.133 0.000 1.205 223 P CA 0.190 63.365 63.100 0.126 0.000 0.765 223 P CB 0.851 32.610 31.700 0.097 0.000 0.828 224 L N 2.296 123.582 121.223 0.105 0.000 2.456 224 L HA -0.121 4.219 4.340 -0.001 0.000 0.224 224 L C 1.368 178.280 176.870 0.071 0.000 1.148 224 L CA 1.191 56.083 54.840 0.087 0.000 0.825 224 L CB -0.574 41.526 42.059 0.069 0.000 0.937 224 L HN 0.453 nan 8.230 nan 0.000 0.450 225 D N -1.040 119.401 120.400 0.069 0.000 2.325 225 D HA -0.053 4.587 4.640 -0.001 0.000 0.225 225 D C 1.596 177.934 176.300 0.062 0.000 1.096 225 D CA 0.161 54.195 54.000 0.057 0.000 0.844 225 D CB 0.204 41.034 40.800 0.049 0.000 0.925 225 D HN 0.247 nan 8.370 nan 0.000 0.513 226 R N -0.995 119.554 120.500 0.081 0.000 2.612 226 R HA 0.192 4.532 4.340 -0.001 0.000 0.260 226 R C -0.109 176.251 176.300 0.100 0.000 0.943 226 R CA -0.576 55.578 56.100 0.090 0.000 1.036 226 R CB 0.626 30.991 30.300 0.109 0.000 1.520 226 R HN 0.038 nan 8.270 nan 0.000 0.563 227 L N 2.219 123.504 121.223 0.104 0.000 2.361 227 L HA 0.336 4.675 4.340 -0.001 0.000 0.278 227 L C -0.373 176.528 176.870 0.051 0.000 1.113 227 L CA -0.126 54.765 54.840 0.085 0.000 0.849 227 L CB 0.934 43.049 42.059 0.094 0.000 1.155 227 L HN 0.057 nan 8.230 nan 0.000 0.452 228 A N 5.768 128.611 122.820 0.038 0.000 2.260 228 A HA 0.640 4.960 4.320 -0.001 0.000 0.308 228 A C -0.713 176.888 177.584 0.027 0.000 1.254 228 A CA -0.540 51.516 52.037 0.031 0.000 0.874 228 A CB 0.548 19.568 19.000 0.033 0.000 1.153 228 A HN 0.531 nan 8.150 nan 0.000 0.527 229 V N 3.792 123.725 119.914 0.032 0.000 2.398 229 V HA 0.457 4.576 4.120 -0.001 0.000 0.286 229 V C -0.243 175.880 176.094 0.049 0.000 1.026 229 V CA -0.385 61.946 62.300 0.052 0.000 0.868 229 V CB 1.444 33.277 31.823 0.017 0.000 0.982 229 V HN 0.628 nan 8.190 nan 0.000 0.443 230 V N 3.936 123.900 119.914 0.083 0.000 2.540 230 V HA 0.430 4.550 4.120 -0.001 0.000 0.302 230 V C -0.056 176.090 176.094 0.086 0.000 1.035 230 V CA -0.888 61.448 62.300 0.059 0.000 0.873 230 V CB 2.078 33.922 31.823 0.035 0.000 0.992 230 V HN 0.854 nan 8.190 nan 0.000 0.428 231 N N 3.603 122.334 118.700 0.051 0.000 2.411 231 N HA 0.190 4.930 4.740 -0.001 0.000 0.259 231 N C -0.659 174.875 175.510 0.040 0.000 1.103 231 N CA -0.021 53.062 53.050 0.056 0.000 0.954 231 N CB 1.426 39.928 38.487 0.024 0.000 1.085 231 N HN 0.428 nan 8.380 nan 0.000 0.485 232 V N 3.979 123.922 119.914 0.047 0.000 2.408 232 V HA 0.081 4.201 4.120 -0.001 0.000 0.267 232 V C 0.710 176.802 176.094 -0.004 0.000 1.047 232 V CA -0.729 61.573 62.300 0.003 0.000 0.937 232 V CB 0.486 32.292 31.823 -0.029 0.000 0.999 232 V HN 0.566 nan 8.190 nan 0.000 0.472 233 K N 4.365 124.758 120.400 -0.011 0.000 2.448 233 K HA 0.065 4.384 4.320 -0.001 0.000 0.278 233 K C -0.020 176.566 176.600 -0.024 0.000 1.009 233 K CA 0.055 56.334 56.287 -0.013 0.000 0.995 233 K CB 0.165 32.657 32.500 -0.013 0.000 0.917 233 K HN 0.617 nan 8.250 nan 0.000 0.481 234 D N 2.150 122.538 120.400 -0.020 0.000 2.449 234 D HA 0.068 4.708 4.640 -0.001 0.000 0.236 234 D C -0.496 175.786 176.300 -0.030 0.000 1.149 234 D CA 0.937 54.920 54.000 -0.028 0.000 0.878 234 D CB 0.234 41.024 40.800 -0.018 0.000 1.198 234 D HN 0.571 nan 8.370 nan 0.000 0.446 235 N N 1.292 119.969 118.700 -0.038 0.000 3.127 235 N HA 0.121 4.860 4.740 -0.001 0.000 0.239 235 N C -2.487 173.001 175.510 -0.038 0.000 1.200 235 N CA -0.959 52.072 53.050 -0.033 0.000 0.924 235 N CB 1.234 39.700 38.487 -0.035 0.000 1.583 235 N HN -0.019 nan 8.380 nan 0.000 0.645 236 P HA -0.306 nan 4.420 nan 0.000 0.229 236 P C -1.653 175.629 177.300 -0.030 0.000 0.812 236 P CA 2.414 65.500 63.100 -0.023 0.000 1.111 236 P CB -0.867 30.823 31.700 -0.016 0.000 0.658 237 P HA -0.013 nan 4.420 nan 0.000 0.251 237 P C 0.279 177.528 177.300 -0.084 0.000 1.251 237 P CA 1.267 64.346 63.100 -0.036 0.000 0.763 237 P CB -0.652 31.034 31.700 -0.023 0.000 1.067 238 M N -2.528 117.012 119.600 -0.099 0.000 2.523 238 M HA 0.551 5.031 4.480 -0.001 0.000 0.287 238 M C -1.732 174.499 176.300 -0.116 0.000 1.160 238 M CA -0.908 54.294 55.300 -0.163 0.000 0.902 238 M CB 2.870 35.349 32.600 -0.203 0.000 1.752 238 M HN -0.344 nan 8.290 nan 0.000 0.504 239 K N 1.017 121.341 120.400 -0.127 0.000 2.546 239 K HA 0.371 4.690 4.320 -0.001 0.000 0.264 239 K C -1.280 175.286 176.600 -0.057 0.000 0.937 239 K CA -0.304 55.942 56.287 -0.068 0.000 0.833 239 K CB 1.560 34.037 32.500 -0.038 0.000 1.378 239 K HN 0.923 nan 8.250 nan 0.000 0.432 240 D N 2.046 122.439 120.400 -0.010 0.000 2.689 240 D HA -0.215 4.425 4.640 -0.001 0.000 0.237 240 D C -0.622 175.706 176.300 0.048 0.000 1.148 240 D CA 1.294 55.316 54.000 0.037 0.000 0.656 240 D CB -0.897 39.937 40.800 0.055 0.000 1.050 240 D HN 0.466 nan 8.370 nan 0.000 0.426 241 M N -0.093 119.514 119.600 0.011 0.000 2.528 241 M HA 0.640 5.120 4.480 -0.001 0.000 0.321 241 M C 0.055 176.414 176.300 0.098 0.000 1.153 241 M CA -1.002 54.283 55.300 -0.026 0.000 0.951 241 M CB 1.973 34.468 32.600 -0.175 0.000 1.705 241 M HN 0.065 nan 8.290 nan 0.000 0.451 242 F N -0.331 119.589 119.950 -0.050 0.000 2.603 242 F HA 0.854 5.381 4.527 -0.001 0.000 0.317 242 F C -1.195 174.582 175.800 -0.039 0.000 1.066 242 F CA -1.106 56.868 58.000 -0.042 0.000 0.941 242 F CB 1.380 40.357 39.000 -0.038 0.000 1.291 242 F HN 0.470 nan 8.300 nan 0.000 0.472 243 K N 2.604 123.069 120.400 0.108 0.000 2.371 243 K HA 0.689 5.009 4.320 -0.001 0.000 0.251 243 K C -2.022 174.615 176.600 0.062 0.000 0.934 243 K CA -0.708 55.556 56.287 -0.038 0.000 0.798 243 K CB 1.911 34.366 32.500 -0.074 0.000 1.204 243 K HN 0.915 nan 8.250 nan 0.000 0.427 244 L N 5.385 126.600 121.223 -0.013 0.000 2.307 244 L HA 0.486 4.825 4.340 -0.001 0.000 0.284 244 L C -0.763 176.056 176.870 -0.086 0.000 1.023 244 L CA -1.017 53.802 54.840 -0.036 0.000 0.810 244 L CB 1.224 43.215 42.059 -0.114 0.000 1.231 244 L HN 0.522 nan 8.230 nan 0.000 0.423 245 L N 5.247 126.441 121.223 -0.049 0.000 2.343 245 L HA 0.609 4.949 4.340 -0.001 0.000 0.278 245 L C -0.495 176.392 176.870 0.029 0.000 0.996 245 L CA -0.152 54.683 54.840 -0.010 0.000 0.831 245 L CB 1.894 43.964 42.059 0.018 0.000 1.232 245 L HN 0.672 nan 8.230 nan 0.000 0.413 246 M N 5.253 124.898 119.600 0.076 0.000 2.291 246 M HA 0.274 4.754 4.480 -0.001 0.000 0.202 246 M C -0.886 175.550 176.300 0.228 0.000 0.985 246 M CA -0.571 54.836 55.300 0.178 0.000 0.993 246 M CB 1.078 33.830 32.600 0.253 0.000 2.723 246 M HN 0.432 nan 8.290 nan 0.000 0.402 247 F N 7.129 127.129 119.950 0.083 0.000 2.569 247 F HA 0.066 4.593 4.527 -0.000 0.000 0.372 247 F C -1.332 174.525 175.800 0.095 0.000 1.053 247 F CA 0.206 58.250 58.000 0.074 0.000 1.282 247 F CB 0.557 39.588 39.000 0.052 0.000 0.956 247 F HN 0.491 nan 8.300 nan 0.000 0.591 248 P HA 0.021 nan 4.420 nan 0.000 0.253 248 P C -0.677 176.416 177.300 -0.345 0.000 1.260 248 P CA 0.490 62.878 63.100 -1.186 0.000 0.800 248 P CB 0.047 31.007 31.700 -1.233 0.000 1.162 249 E N -0.088 120.042 120.200 -0.118 0.000 2.280 249 E HA 0.472 4.821 4.350 -0.001 0.000 0.264 249 E C -0.441 176.157 176.600 -0.005 0.000 1.064 249 E CA -0.676 55.714 56.400 -0.017 0.000 0.900 249 E CB 0.997 30.758 29.700 0.101 0.000 1.123 249 E HN -0.152 nan 8.360 nan 0.000 0.418 250 S N 1.195 116.875 115.700 -0.034 0.000 2.689 250 S HA 0.396 4.865 4.470 -0.001 0.000 0.274 250 S C -1.414 173.111 174.600 -0.125 0.000 1.176 250 S CA -0.736 57.424 58.200 -0.066 0.000 1.014 250 S CB 0.405 63.572 63.200 -0.054 0.000 1.071 250 S HN 0.450 nan 8.310 nan 0.000 0.478 251 R N 3.597 123.974 120.500 -0.206 0.000 2.628 251 R HA 0.609 4.949 4.340 -0.001 0.000 0.288 251 R C -0.977 174.947 176.300 -0.627 0.000 0.980 251 R CA -0.720 55.132 56.100 -0.414 0.000 0.891 251 R CB 1.882 31.908 30.300 -0.457 0.000 1.188 251 R HN 0.524 nan 8.270 nan 0.000 0.450 252 I N 2.949 123.103 120.570 -0.692 0.000 2.412 252 I HA 0.425 4.595 4.170 -0.001 0.000 0.296 252 I C -0.922 174.653 176.117 -0.902 0.000 0.987 252 I CA -0.621 60.302 61.300 -0.628 0.000 1.180 252 I CB 1.071 38.875 38.000 -0.327 0.000 1.340 252 I HN 0.381 nan 8.210 nan 0.000 0.455 253 F N 3.662 123.283 119.950 -0.548 0.000 2.540 253 F HA 0.421 4.947 4.527 -0.001 0.000 0.317 253 F C 0.068 175.567 175.800 -0.502 0.000 1.104 253 F CA -0.670 56.976 58.000 -0.591 0.000 0.913 253 F CB 1.719 40.109 39.000 -1.017 0.000 1.170 253 F HN 0.341 nan 8.300 nan 0.000 0.450 254 Q N 2.338 122.120 119.800 -0.031 0.000 2.322 254 Q HA 0.786 5.125 4.340 -0.001 0.000 0.265 254 Q C -1.082 174.964 176.000 0.078 0.000 0.985 254 Q CA -0.783 55.014 55.803 -0.011 0.000 0.849 254 Q CB 1.785 30.530 28.738 0.012 0.000 1.274 254 Q HN 0.865 nan 8.270 nan 0.000 0.449 255 A N 2.954 125.852 122.820 0.130 0.000 2.286 255 A HA 0.139 4.459 4.320 -0.001 0.000 0.286 255 A C 0.681 178.355 177.584 0.149 0.000 1.097 255 A CA -0.255 51.903 52.037 0.203 0.000 0.821 255 A CB 0.779 19.961 19.000 0.303 0.000 1.076 255 A HN 1.081 nan 8.150 nan 0.000 0.490 256 E N 0.352 120.645 120.200 0.155 0.000 2.267 256 E HA -0.211 4.139 4.350 -0.001 0.000 0.197 256 E C 0.138 176.795 176.600 0.096 0.000 0.998 256 E CA 1.593 58.062 56.400 0.116 0.000 0.830 256 E CB -0.136 29.632 29.700 0.114 0.000 0.751 256 E HN 0.810 nan 8.360 nan 0.000 0.491 257 N N -3.590 115.174 118.700 0.106 0.000 3.227 257 N HA 0.202 4.941 4.740 -0.001 0.000 0.241 257 N C 0.008 175.571 175.510 0.088 0.000 1.480 257 N CA -0.082 53.017 53.050 0.081 0.000 0.886 257 N CB 0.378 38.905 38.487 0.067 0.000 1.406 257 N HN -0.119 nan 8.380 nan 0.000 0.514 258 A N 0.046 122.904 122.820 0.064 0.000 1.978 258 A HA -0.211 4.109 4.320 -0.001 0.000 0.220 258 A C 1.863 179.484 177.584 0.061 0.000 1.170 258 A CA 1.993 54.065 52.037 0.058 0.000 0.636 258 A CB -0.769 18.254 19.000 0.038 0.000 0.810 258 A HN 0.732 nan 8.150 nan 0.000 0.448 259 K N -0.407 120.028 120.400 0.058 0.000 2.002 259 K HA -0.124 4.196 4.320 -0.001 0.000 0.209 259 K C 1.794 178.435 176.600 0.068 0.000 1.048 259 K CA 1.599 57.916 56.287 0.050 0.000 0.930 259 K CB -0.262 32.264 32.500 0.044 0.000 0.714 259 K HN 0.358 nan 8.250 nan 0.000 0.438 260 I N 2.103 122.739 120.570 0.111 0.000 2.264 260 I HA -0.269 3.900 4.170 -0.001 0.000 0.248 260 I C 2.408 178.665 176.117 0.234 0.000 1.111 260 I CA 1.443 62.847 61.300 0.173 0.000 1.382 260 I CB -1.183 36.961 38.000 0.241 0.000 1.060 260 I HN 0.329 nan 8.210 nan 0.000 0.418 261 K N 1.253 121.784 120.400 0.218 0.000 2.032 261 K HA -0.230 4.090 4.320 -0.001 0.000 0.209 261 K C 2.393 179.040 176.600 0.077 0.000 1.048 261 K CA 1.575 57.990 56.287 0.213 0.000 0.927 261 K CB -0.115 32.487 32.500 0.170 0.000 0.712 261 K HN 0.118 nan 8.250 nan 0.000 0.441 262 R N 0.826 121.348 120.500 0.035 0.000 2.105 262 R HA -0.153 4.186 4.340 -0.001 0.000 0.239 262 R C 1.897 178.146 176.300 -0.086 0.000 1.135 262 R CA 1.923 58.009 56.100 -0.023 0.000 0.967 262 R CB -0.048 30.245 30.300 -0.011 0.000 0.861 262 R HN 0.347 nan 8.270 nan 0.000 0.442 263 E N -0.779 119.356 120.200 -0.109 0.000 2.077 263 E HA -0.226 4.123 4.350 -0.001 0.000 0.193 263 E C 1.737 178.022 176.600 -0.525 0.000 0.989 263 E CA 1.547 57.780 56.400 -0.279 0.000 0.800 263 E CB -0.189 29.368 29.700 -0.239 0.000 0.746 263 E HN 0.464 nan 8.360 nan 0.000 0.452 264 W N 1.068 122.055 121.300 -0.522 0.000 2.355 264 W HA -0.141 4.518 4.660 -0.001 0.000 0.309 264 W C 2.143 178.320 176.519 -0.570 0.000 1.206 264 W CA 0.642 57.590 57.345 -0.662 0.000 1.284 264 W CB -0.277 28.773 29.460 -0.683 0.000 1.145 264 W HN 0.051 nan 8.180 nan 0.000 0.502 265 L N -0.086 121.002 121.223 -0.225 0.000 2.046 265 L HA -0.216 4.124 4.340 -0.001 0.000 0.208 265 L C 2.221 178.997 176.870 -0.156 0.000 1.077 265 L CA 1.450 56.165 54.840 -0.209 0.000 0.747 265 L CB -0.841 41.127 42.059 -0.153 0.000 0.896 265 L HN 0.087 nan 8.230 nan 0.000 0.432 266 E N -0.059 120.043 120.200 -0.162 0.000 2.028 266 E HA -0.183 4.166 4.350 -0.001 0.000 0.191 266 E C 2.225 178.735 176.600 -0.149 0.000 0.988 266 E CA 1.619 57.939 56.400 -0.133 0.000 0.799 266 E CB -0.207 29.420 29.700 -0.122 0.000 0.755 266 E HN 0.458 nan 8.360 nan 0.000 0.447 267 V N 0.030 119.800 119.914 -0.240 0.000 2.343 267 V HA -0.244 3.875 4.120 -0.001 0.000 0.247 267 V C 2.321 178.336 176.094 -0.130 0.000 1.051 267 V CA 1.435 63.605 62.300 -0.217 0.000 1.036 267 V CB -0.902 30.686 31.823 -0.391 0.000 0.654 267 V HN 0.148 nan 8.190 nan 0.000 0.451 268 L N 0.119 121.268 121.223 -0.123 0.000 2.012 268 L HA -0.169 4.170 4.340 -0.001 0.000 0.210 268 L C 2.871 179.716 176.870 -0.041 0.000 1.073 268 L CA 2.251 57.060 54.840 -0.052 0.000 0.748 268 L CB -0.611 41.413 42.059 -0.059 0.000 0.891 268 L HN 0.452 nan 8.230 nan 0.000 0.431 269 E N 0.546 120.711 120.200 -0.058 0.000 2.028 269 E HA -0.271 4.079 4.350 -0.001 0.000 0.191 269 E C 2.000 178.586 176.600 -0.024 0.000 0.988 269 E CA 1.739 58.117 56.400 -0.037 0.000 0.799 269 E CB -0.136 29.538 29.700 -0.043 0.000 0.755 269 E HN 0.421 nan 8.360 nan 0.000 0.447 270 E N -0.754 119.426 120.200 -0.034 0.000 2.058 270 E HA -0.221 4.129 4.350 -0.001 0.000 0.194 270 E C 1.898 178.499 176.600 0.001 0.000 0.997 270 E CA 1.800 58.190 56.400 -0.017 0.000 0.801 270 E CB -0.397 29.288 29.700 -0.024 0.000 0.746 270 E HN 0.333 nan 8.360 nan 0.000 0.450 271 T N -0.140 114.411 114.554 -0.004 0.000 2.915 271 T HA -0.124 4.225 4.350 -0.001 0.000 0.269 271 T C 1.786 176.500 174.700 0.022 0.000 1.071 271 T CA 1.505 63.613 62.100 0.014 0.000 1.132 271 T CB -0.157 68.710 68.868 -0.003 0.000 0.878 271 T HN 0.133 nan 8.240 nan 0.000 0.479 272 K N 0.194 120.603 120.400 0.015 0.000 2.116 272 K HA 0.001 4.321 4.320 -0.001 0.000 0.203 272 K C 2.597 179.210 176.600 0.021 0.000 1.052 272 K CA 0.396 56.696 56.287 0.021 0.000 0.952 272 K CB -0.214 32.296 32.500 0.017 0.000 0.729 272 K HN 0.117 nan 8.250 nan 0.000 0.446 273 R N 0.993 121.501 120.500 0.014 0.000 2.073 273 R HA -0.102 4.238 4.340 -0.001 0.000 0.234 273 R C 2.002 178.314 176.300 0.021 0.000 1.134 273 R CA 1.642 57.750 56.100 0.014 0.000 0.952 273 R CB -0.558 29.747 30.300 0.008 0.000 0.850 273 R HN 0.283 nan 8.270 nan 0.000 0.433 274 A N 1.302 124.138 122.820 0.027 0.000 1.940 274 A HA -0.181 4.138 4.320 -0.001 0.000 0.219 274 A C 2.178 179.785 177.584 0.038 0.000 1.176 274 A CA 1.383 53.441 52.037 0.036 0.000 0.631 274 A CB -0.611 18.420 19.000 0.052 0.000 0.814 274 A HN 0.373 nan 8.150 nan 0.000 0.446 275 L N -0.243 121.003 121.223 0.039 0.000 2.083 275 L HA -0.087 4.253 4.340 -0.001 0.000 0.209 275 L C 2.477 179.366 176.870 0.032 0.000 1.083 275 L CA 2.478 57.341 54.840 0.039 0.000 0.752 275 L CB -0.569 41.514 42.059 0.040 0.000 0.899 275 L HN 0.275 nan 8.230 nan 0.000 0.433 276 S N -0.370 115.347 115.700 0.027 0.000 2.355 276 S HA -0.158 4.312 4.470 -0.001 0.000 0.222 276 S C 1.560 176.172 174.600 0.021 0.000 1.031 276 S CA 1.196 59.410 58.200 0.023 0.000 0.993 276 S CB -0.427 62.785 63.200 0.019 0.000 0.859 276 S HN 0.541 nan 8.310 nan 0.000 0.453 277 D N 1.334 121.746 120.400 0.020 0.000 2.106 277 D HA -0.149 4.490 4.640 -0.001 0.000 0.191 277 D C 1.911 178.222 176.300 0.019 0.000 0.997 277 D CA 1.140 55.151 54.000 0.018 0.000 0.834 277 D CB -0.363 40.448 40.800 0.018 0.000 0.956 277 D HN 0.367 nan 8.370 nan 0.000 0.448 278 K N 0.870 121.285 120.400 0.024 0.000 2.074 278 K HA -0.176 4.144 4.320 -0.001 0.000 0.209 278 K C 2.109 178.722 176.600 0.022 0.000 1.048 278 K CA 1.226 57.528 56.287 0.024 0.000 0.926 278 K CB 0.076 32.595 32.500 0.032 0.000 0.713 278 K HN 0.023 nan 8.250 nan 0.000 0.444 279 R N -0.168 120.346 120.500 0.022 0.000 2.148 279 R HA -0.077 4.263 4.340 -0.001 0.000 0.223 279 R C 2.508 178.818 176.300 0.017 0.000 1.088 279 R CA 1.182 57.294 56.100 0.020 0.000 0.985 279 R CB -0.178 30.136 30.300 0.022 0.000 0.880 279 R HN 0.233 nan 8.270 nan 0.000 0.451 280 R N 1.283 121.793 120.500 0.016 0.000 2.115 280 R HA -0.010 4.330 4.340 -0.001 0.000 0.226 280 R C 1.267 177.574 176.300 0.011 0.000 1.100 280 R CA 0.916 57.024 56.100 0.013 0.000 0.980 280 R CB 0.192 30.499 30.300 0.012 0.000 0.875 280 R HN 0.057 nan 8.270 nan 0.000 0.445 281 R N 0.149 120.657 120.500 0.012 0.000 2.404 281 R HA 0.021 4.361 4.340 -0.001 0.000 0.236 281 R C 0.478 176.785 176.300 0.011 0.000 1.044 281 R CA 0.382 56.488 56.100 0.011 0.000 1.133 281 R CB 0.254 30.561 30.300 0.011 0.000 1.142 281 R HN 0.401 nan 8.270 nan 0.000 0.512 282 E N -0.348 119.859 120.200 0.011 0.000 2.399 282 E HA 0.053 4.403 4.350 -0.001 0.000 0.206 282 E C 0.241 176.847 176.600 0.010 0.000 0.812 282 E CA 0.080 56.486 56.400 0.011 0.000 1.138 282 E CB 0.680 30.389 29.700 0.014 0.000 1.140 282 E HN 0.220 nan 8.360 nan 0.000 0.536 283 Q N 0.000 119.806 119.800 0.010 0.000 2.315 283 Q HA 0.000 4.340 4.340 -0.001 0.000 0.214 283 Q CA 0.000 55.809 55.803 0.009 0.000 1.022 283 Q CB 0.000 28.744 28.738 0.010 0.000 1.108 283 Q HN 0.000 nan 8.270 nan 0.000 0.481