==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNOGLOBULIN 14-MAR-98 12E8 . COMPND 2 MOLECULE: IGG1-KAPPA 2E8 FAB (LIGHT CHAIN); . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR B.RUPP,S.TRAKHANOV . 870 4 8 8 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 39625.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 564 64.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 22 2.5 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 340 39.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 9 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 93 10.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 70 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 21 2.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 5 11 11 4 6 16 2 2 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 1 8 3 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 L D 0 0 140 0, 0.0 2,-0.5 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 137.7 74.7 34.8 -6.7 2 2 L I - 0 0 8 93,-0.5 2,-0.5 91,-0.2 88,-0.1 -0.719 360.0-139.9 -85.3 126.4 72.6 37.2 -4.6 3 3 L V - 0 0 84 -2,-0.5 23,-1.7 94,-0.1 2,-0.6 -0.802 9.9-159.6 -90.7 126.7 69.3 38.0 -6.2 4 4 L M E -A 25 0A 9 94,-0.5 2,-0.6 -2,-0.5 96,-0.4 -0.964 14.9-158.5-106.6 110.6 68.1 41.6 -5.9 5 5 L T E -A 24 0A 69 19,-3.4 19,-2.6 -2,-0.6 2,-0.7 -0.859 21.0-178.6-102.0 122.1 64.3 41.7 -6.5 6 6 L Q - 0 0 8 -2,-0.6 17,-0.1 17,-0.2 94,-0.1 -0.924 10.9-169.0-113.1 95.4 62.4 44.8 -7.6 7 7 L S + 0 0 81 -2,-0.7 2,-0.7 1,-0.1 -1,-0.1 0.708 67.0 71.3 -58.6 -27.7 58.8 43.5 -7.7 8 8 L Q + 0 0 100 14,-0.3 -1,-0.1 1,-0.2 3,-0.1 -0.844 51.6 178.8 -99.5 111.3 57.4 46.5 -9.4 9 9 L K S S+ 0 0 115 -2,-0.7 94,-2.9 1,-0.2 2,-0.3 0.861 75.7 19.2 -74.3 -36.2 58.4 46.9 -13.1 10 10 L F E -d 103 0B 83 92,-0.2 2,-0.3 75,-0.0 94,-0.2 -0.972 59.9-174.5-136.2 151.5 56.3 50.1 -13.4 11 11 L M E -d 104 0B 46 92,-2.0 94,-3.0 -2,-0.3 2,-0.4 -0.973 19.4-139.9-146.5 124.9 54.8 52.8 -11.1 12 12 L S E +d 105 0B 54 -2,-0.3 2,-0.3 92,-0.2 94,-0.2 -0.734 33.3 168.1 -85.6 137.0 52.5 55.6 -12.2 13 13 L T E -d 106 0B 2 92,-3.1 94,-2.1 -2,-0.4 2,-0.3 -0.834 26.3-138.3-141.4 172.9 53.3 58.9 -10.3 14 14 L S > - 0 0 39 -2,-0.3 3,-2.4 92,-0.2 64,-0.2 -0.972 39.1 -91.7-136.2 147.1 52.5 62.6 -10.4 15 15 L V T 3 S+ 0 0 77 -2,-0.3 64,-0.2 1,-0.3 3,-0.1 -0.401 117.3 32.6 -55.7 129.2 54.7 65.6 -9.9 16 16 L G T 3 S+ 0 0 40 62,-2.3 61,-0.4 1,-0.4 -1,-0.3 0.056 92.3 124.7 104.9 -19.1 54.5 66.4 -6.1 17 17 L D < - 0 0 62 -3,-2.4 61,-2.9 60,-0.2 2,-0.6 -0.307 62.1-126.7 -74.3 156.3 54.0 62.8 -5.0 18 18 L R - 0 0 78 435,-0.6 2,-0.4 59,-0.2 58,-0.2 -0.879 35.8-168.6 -99.5 124.8 56.1 60.9 -2.5 19 19 L V E - B 0 75A 5 56,-2.9 56,-3.0 -2,-0.6 2,-0.4 -0.945 12.4-166.2-124.0 138.9 57.3 57.7 -4.2 20 20 L S E - B 0 74A 39 -2,-0.4 2,-0.4 54,-0.2 54,-0.2 -0.986 1.5-165.1-124.8 136.9 58.9 54.7 -2.8 21 21 L I E - B 0 73A 2 52,-2.8 52,-2.7 -2,-0.4 2,-0.2 -0.975 14.8-144.9-122.6 128.4 60.6 51.9 -4.7 22 22 L T E - B 0 72A 65 -2,-0.4 2,-0.3 50,-0.2 -14,-0.3 -0.634 16.5-178.9 -98.9 154.5 61.4 48.5 -3.1 23 23 L a E - B 0 71A 1 48,-2.2 48,-2.6 -2,-0.2 2,-0.4 -0.963 8.9-161.7-149.7 127.7 64.3 46.1 -3.5 24 24 L K E -AB 5 70A 119 -19,-2.6 -19,-3.4 -2,-0.3 2,-0.3 -0.929 7.1-149.3-118.1 134.2 64.7 42.8 -1.7 25 25 L A E -A 4 0A 4 44,-2.6 -21,-0.2 -2,-0.4 4,-0.1 -0.782 13.2-140.8 -99.1 146.2 68.0 40.9 -1.3 26 26 L S S S+ 0 0 70 -23,-1.7 2,-0.3 -2,-0.3 -22,-0.1 0.590 88.3 32.2 -80.5 -10.6 67.9 37.1 -1.1 27 27 L Q S S- 0 0 101 -24,-0.2 2,-0.6 41,-0.1 -2,-0.1 -0.905 104.0 -79.9-139.4 161.9 70.7 37.2 1.5 28 28 L N + 0 0 114 -2,-0.3 41,-0.2 1,-0.2 3,-0.1 -0.580 47.0 169.5 -67.3 114.7 71.8 39.6 4.3 29 29 L V > - 0 0 3 39,-2.5 3,-1.9 -2,-0.6 2,-0.3 0.303 30.6-144.0-109.7 8.8 73.7 42.4 2.5 30 30 L G T 3 - 0 0 31 1,-0.3 38,-1.3 38,-0.2 -1,-0.3 -0.502 64.5 -41.7 66.4-127.4 74.0 44.7 5.5 31 31 L T T 3 S+ 0 0 74 -2,-0.3 2,-2.5 36,-0.2 -1,-0.3 0.146 102.4 124.4-116.4 16.5 73.7 48.3 4.2 32 32 L A < + 0 0 6 -3,-1.9 59,-2.2 18,-0.2 60,-0.4 -0.381 42.8 115.9 -78.1 63.9 75.9 47.5 1.2 33 33 L V E -E 90 0B 0 -2,-2.5 18,-2.8 57,-0.2 2,-0.3 -0.971 42.7-167.5-135.5 145.5 73.4 48.6 -1.4 34 34 L A E -E 89 0B 0 55,-2.3 55,-1.5 -2,-0.3 2,-0.4 -0.933 11.1-143.5-130.5 154.7 73.4 51.4 -3.9 35 35 L W E -EF 88 48B 0 13,-2.6 12,-3.0 -2,-0.3 13,-1.4 -0.971 14.6-164.3-120.9 137.9 70.6 52.9 -6.1 36 36 L Y E -EF 87 46B 0 51,-2.7 51,-2.2 -2,-0.4 2,-0.4 -0.938 9.5-147.0-122.8 148.4 71.0 54.1 -9.7 37 37 L Q E -EF 86 45B 18 8,-2.4 8,-1.7 -2,-0.3 2,-0.4 -0.905 19.1-176.6-111.7 135.9 68.9 56.3 -12.0 38 38 L Q E -E 85 0B 14 47,-3.1 47,-3.1 -2,-0.4 5,-0.1 -0.990 11.9-167.8-139.6 136.2 68.8 55.8 -15.7 39 39 L K > - 0 0 54 -2,-0.4 3,-2.1 4,-0.3 45,-0.1 -0.771 51.2 -67.1-115.7 158.1 67.1 57.5 -18.6 40 40 L P T 3 S- 0 0 114 0, 0.0 -1,-0.1 0, 0.0 3,-0.1 -0.155 116.1 -6.8 -55.1 126.9 66.8 56.1 -22.2 41 41 L G T 3 S+ 0 0 65 1,-0.2 2,-0.3 -3,-0.0 286,-0.1 0.642 112.5 111.6 65.7 12.3 70.1 55.8 -24.0 42 42 L Q S < S- 0 0 91 -3,-2.1 -1,-0.2 284,-0.1 284,-0.1 -0.798 76.5 -89.4-117.1 161.5 72.0 57.6 -21.2 43 43 L S - 0 0 23 -2,-0.3 -4,-0.3 282,-0.2 284,-0.2 -0.445 44.8-113.3 -63.8 136.8 74.5 56.5 -18.5 44 44 L P - 0 0 3 0, 0.0 281,-0.5 0, 0.0 2,-0.4 -0.202 35.5-143.2 -55.7 160.2 73.1 55.2 -15.3 45 45 L K E -F 37 0B 95 -8,-1.7 -8,-2.4 279,-0.1 2,-0.4 -0.999 11.8-114.7-137.1 139.0 73.9 57.5 -12.3 46 46 L L E +F 36 0B 6 277,-0.5 -10,-0.2 -2,-0.4 3,-0.1 -0.503 41.1 159.2 -72.9 124.6 74.7 56.9 -8.7 47 47 L M E + 0 0 11 -12,-3.0 8,-2.5 1,-0.4 2,-0.3 0.821 62.4 13.1-110.8 -50.5 72.0 58.2 -6.4 48 48 L I E +FG 35 54B 0 -13,-1.4 -13,-2.6 6,-0.2 -1,-0.4 -0.964 61.0 179.7-130.8 146.6 72.2 56.4 -3.1 49 49 L Y E > + G 0 53B 54 4,-2.5 4,-2.5 -2,-0.3 3,-0.4 -0.928 66.9 31.4-142.6 161.5 75.0 54.3 -1.6 50 50 L S T 4 S- 0 0 38 -2,-0.3 2,-1.7 1,-0.3 -18,-0.2 0.831 122.2 -81.2 56.6 35.5 75.4 52.4 1.7 51 51 L A T 4 S+ 0 0 0 -18,-2.8 -1,-0.3 1,-0.2 21,-0.2 -0.123 126.1 31.1 64.0 -31.8 71.6 52.1 1.6 52 52 L S T 4 S+ 0 0 68 -2,-1.7 2,-0.5 -3,-0.4 12,-0.4 0.392 88.4 104.6-137.2 4.1 70.8 55.6 2.9 53 53 L N E < -G 49 0B 73 -4,-2.5 -4,-2.5 10,-0.1 2,-0.3 -0.783 61.8-132.8 -97.1 133.7 73.6 58.0 1.9 54 54 L R E -G 48 0B 89 -2,-0.5 -6,-0.2 -6,-0.2 3,-0.1 -0.604 23.7-124.6 -82.4 131.4 73.0 60.5 -0.9 55 55 L Y > - 0 0 39 -8,-2.5 3,-1.8 -2,-0.3 -1,-0.1 -0.399 45.8 -74.5 -73.0 153.8 75.8 60.6 -3.5 56 56 L T T 3 S+ 0 0 120 1,-0.2 -1,-0.2 -2,-0.1 -10,-0.0 -0.208 115.9 10.4 -49.0 136.9 77.4 64.1 -4.1 57 57 L G T 3 S+ 0 0 82 1,-0.2 -1,-0.2 -3,-0.1 3,-0.1 0.282 89.1 129.2 79.2 -16.9 75.2 66.4 -6.1 58 58 L V < - 0 0 15 -3,-1.8 -1,-0.2 -11,-0.1 -4,-0.1 -0.571 68.3-100.3 -75.8 140.2 72.0 64.4 -6.0 59 59 L P > - 0 0 48 0, 0.0 3,-1.6 0, 0.0 -1,-0.1 -0.180 25.2-115.0 -63.7 155.1 69.1 66.5 -4.9 60 60 L D T 3 S+ 0 0 128 1,-0.3 -2,-0.1 -3,-0.1 16,-0.0 0.563 104.9 78.9 -67.4 -6.5 67.9 66.3 -1.3 61 61 L R T 3 S+ 0 0 48 16,-0.1 15,-2.4 14,-0.1 2,-0.5 0.597 79.6 80.8 -78.7 -8.5 64.5 64.8 -2.4 62 62 L F E < +C 75 0A 2 -3,-1.6 2,-0.3 13,-0.2 13,-0.2 -0.833 56.4 158.4 -97.1 130.3 66.1 61.4 -2.8 63 63 L T E -C 74 0A 51 11,-2.3 11,-3.0 -2,-0.5 2,-0.3 -0.938 19.7-157.6-141.1 165.0 66.7 59.3 0.3 64 64 L G E +C 73 0A 8 -12,-0.4 2,-0.3 -2,-0.3 9,-0.2 -0.970 11.5 173.7-143.4 157.6 67.1 55.6 0.9 65 65 L S E +C 72 0A 59 7,-2.0 7,-2.3 -2,-0.3 2,-0.1 -0.935 25.5 80.3-153.7 173.1 66.6 53.3 3.8 66 66 L G E -C 71 0A 35 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.397 36.4-156.8 113.6 173.0 66.7 49.6 4.8 67 67 L S E > +C 70 0A 37 3,-1.4 3,-1.3 -2,-0.1 2,-1.2 -0.936 61.9 29.0-176.7 166.3 69.2 47.0 5.8 68 68 L G T 3 S- 0 0 16 -38,-1.3 -39,-2.5 -2,-0.3 -38,-0.2 -0.600 130.7 -25.0 74.0 -96.3 69.5 43.2 5.8 69 69 L T T 3 S+ 0 0 52 -2,-1.2 -44,-2.6 -41,-0.2 2,-0.5 0.523 122.0 73.2-129.2 -11.3 67.2 42.2 3.0 70 70 L D E < +BC 24 67A 88 -3,-1.3 -3,-1.4 -46,-0.2 2,-0.3 -0.944 54.8 170.0-116.4 122.4 64.7 45.0 2.6 71 71 L F E -BC 23 66A 2 -48,-2.6 -48,-2.2 -2,-0.5 2,-0.3 -0.941 11.9-164.7-129.7 152.9 65.6 48.3 1.0 72 72 L T E -BC 22 65A 34 -7,-2.3 -7,-2.0 -2,-0.3 2,-0.5 -0.997 11.9-155.1-144.1 132.3 63.7 51.3 -0.2 73 73 L L E -BC 21 64A 0 -52,-2.7 -52,-2.8 -2,-0.3 2,-0.4 -0.921 22.5-163.8-102.8 128.2 64.5 54.4 -2.4 74 74 L T E -BC 20 63A 7 -11,-3.0 -11,-2.3 -2,-0.5 2,-0.6 -0.954 13.9-166.2-120.3 130.7 62.2 57.3 -1.6 75 75 L I E -BC 19 62A 0 -56,-3.0 -56,-2.9 -2,-0.4 2,-0.4 -0.977 12.2-159.5-111.5 118.0 61.6 60.4 -3.7 76 76 L S S S+ 0 0 40 -15,-2.4 -58,-0.2 -2,-0.6 -59,-0.1 -0.836 76.3 4.9 -93.5 142.6 59.9 63.2 -1.8 77 77 L N S S- 0 0 96 -61,-0.4 -1,-0.3 -2,-0.4 -59,-0.2 0.935 92.6-138.4 48.9 61.8 58.1 65.8 -3.9 78 78 L M - 0 0 0 -61,-2.9 -62,-2.3 -3,-0.4 2,-0.3 -0.110 17.6-154.1 -52.5 141.5 58.7 64.0 -7.2 79 79 L Q > - 0 0 64 -64,-0.2 3,-2.0 -63,-0.1 4,-0.2 -0.778 29.2-105.1-116.2 162.9 59.7 66.3 -10.1 80 80 L S G > S+ 0 0 81 1,-0.3 3,-1.6 -2,-0.3 -1,-0.1 0.849 119.3 58.4 -54.4 -40.6 59.3 65.8 -13.9 81 81 L E G 3 S+ 0 0 117 1,-0.3 -1,-0.3 3,-0.0 -3,-0.0 0.669 92.5 71.6 -64.0 -17.5 63.1 65.1 -14.3 82 82 L D G < + 0 0 2 -3,-2.0 2,-0.8 2,-0.0 -1,-0.3 0.469 65.4 110.4 -75.8 -7.6 62.6 62.1 -11.9 83 83 L L < + 0 0 48 -3,-1.6 2,-0.3 -4,-0.2 22,-0.1 -0.669 55.3 96.3 -72.2 112.1 60.7 60.1 -14.4 84 84 L A S S- 0 0 6 -2,-0.8 20,-2.7 18,-0.1 2,-0.3 -0.955 77.4 -68.6-175.0-178.5 63.2 57.4 -15.2 85 85 L D E -EH 38 103B 43 -47,-3.1 -47,-3.1 -2,-0.3 2,-0.4 -0.705 42.7-164.3 -91.9 144.7 64.3 53.8 -14.5 86 86 L Y E +EH 37 102B 0 16,-2.1 16,-1.8 -2,-0.3 2,-0.3 -0.989 7.9 180.0-128.6 140.3 65.7 53.0 -11.1 87 87 L F E -E 36 0B 6 -51,-2.2 -51,-2.7 -2,-0.4 2,-0.2 -0.999 16.9-141.7-145.7 142.4 67.7 50.0 -10.0 88 88 L a E -E 35 0B 0 -2,-0.3 11,-2.5 -53,-0.2 2,-0.3 -0.568 15.4-168.8 -92.7 166.4 69.3 48.7 -6.8 89 89 L Q E -EI 34 98B 0 -55,-1.5 -55,-2.3 9,-0.2 2,-0.4 -0.972 12.5-143.4-153.6 140.2 72.6 46.9 -6.6 90 90 L Q E +EI 33 97B 0 7,-2.0 7,-0.9 -2,-0.3 -57,-0.2 -0.858 14.7 179.0-104.9 147.6 74.3 45.0 -3.7 91 91 L Y + 0 0 12 -59,-2.2 -58,-0.1 -2,-0.4 3,-0.1 -0.069 48.1 115.9-134.1 32.7 78.1 45.1 -3.3 92 92 L S S S- 0 0 34 -60,-0.4 2,-0.3 1,-0.3 4,-0.1 0.932 90.5 -26.2 -68.4 -41.8 78.2 43.0 -0.2 93 93 L S S S- 0 0 59 2,-0.2 -1,-0.3 227,-0.0 -91,-0.2 -0.969 91.0 -28.9-163.7 170.9 80.2 40.3 -2.1 94 94 L Y S S+ 0 0 92 -2,-0.3 2,-0.1 -3,-0.1 -3,-0.1 -0.462 104.6 40.6 -87.6 153.3 80.8 38.9 -5.5 95 95 L P S S- 0 0 23 0, 0.0 -93,-0.5 0, 0.0 -2,-0.2 0.546 86.0-135.3 -75.9 148.1 79.5 38.6 -8.0 96 96 L L - 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