==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 20-JAN-10 3ADF . COMPND 2 MOLECULE: MONOMERIC AZAMI GREEN; . SOURCE 2 ORGANISM_SCIENTIFIC: GALAXEA FASCICULARIS; . AUTHOR T.EBISAWA,A.YAMAMURA,Y.KAMEDA,K.HAYAKAWA,K.NAGATA,M.TANOKURA . 419 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 18385.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 315 75.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 26 6.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 202 48.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 36 8.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 35 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 13 3.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 2 0 4 0 2 2 6 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A I 0 0 58 0, 0.0 4,-0.1 0, 0.0 106,-0.1 0.000 360.0 360.0 360.0 97.4 -13.8 -13.0 -1.4 2 5 A K - 0 0 115 2,-0.2 29,-0.1 1,-0.1 0, 0.0 -0.247 360.0-111.8 -72.5 162.6 -16.4 -11.6 -3.8 3 6 A P S S+ 0 0 96 0, 0.0 2,-0.4 0, 0.0 28,-0.3 0.761 113.4 41.6 -65.2 -30.9 -15.6 -10.2 -7.3 4 7 A E S S+ 0 0 92 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.986 78.8 179.3-113.0 134.3 -16.6 -6.8 -5.9 5 8 A M E -A 28 0A 1 23,-2.6 23,-2.4 -2,-0.4 103,-0.2 -0.982 20.6-131.3-130.8 145.2 -15.4 -6.0 -2.4 6 9 A K E -Ab 27 108A 90 101,-3.5 103,-3.1 -2,-0.3 2,-0.4 -0.582 17.3-149.8 -87.7 158.6 -15.9 -2.9 -0.2 7 10 A I E +Ab 26 109A 3 19,-3.1 19,-2.2 101,-0.2 2,-0.4 -0.993 18.5 169.9-129.1 135.7 -13.1 -1.1 1.7 8 11 A K E +Ab 25 110A 119 101,-1.8 103,-1.9 -2,-0.4 2,-0.3 -0.976 15.5 162.5-139.4 133.7 -13.2 0.9 4.9 9 12 A L E -Ab 24 111A 7 15,-1.9 15,-1.9 -2,-0.4 2,-0.4 -0.961 28.2-151.0-141.9 161.8 -10.0 2.0 6.7 10 13 A C E -Ab 23 112A 36 101,-1.8 103,-2.5 -2,-0.3 2,-0.5 -0.978 12.3-162.7-131.4 126.0 -8.7 4.4 9.3 11 14 A M E -Ab 22 113A 0 11,-3.9 11,-2.5 -2,-0.4 2,-0.4 -0.937 4.7-172.4-104.7 125.9 -5.1 5.7 9.2 12 15 A R E +Ab 21 114A 120 101,-2.6 103,-1.9 -2,-0.5 2,-0.3 -0.955 27.0 145.7-114.4 131.4 -3.6 7.2 12.4 13 16 A G E -Ab 20 115A 3 7,-2.4 7,-2.1 -2,-0.4 2,-0.3 -0.967 40.9-145.1-160.6 172.0 -0.3 8.9 11.9 14 17 A T E -Ab 19 116A 5 101,-1.8 103,-2.3 -2,-0.3 2,-0.3 -0.988 11.5-174.1-146.2 143.4 2.5 11.4 12.5 15 18 A V E > S-Ab 18 117A 2 3,-1.4 3,-1.9 -2,-0.3 103,-0.2 -0.937 79.9 -15.0-144.0 118.0 4.9 13.0 10.1 16 19 A N T 3 S- 0 0 23 101,-2.4 102,-0.1 -2,-0.3 3,-0.1 0.909 132.2 -52.7 49.7 40.8 7.7 15.2 11.3 17 20 A G T 3 S+ 0 0 24 100,-0.5 2,-0.6 1,-0.2 -1,-0.3 0.287 108.3 128.1 86.3 -11.9 5.8 15.5 14.5 18 21 A H E < -A 15 0A 65 -3,-1.9 -3,-1.4 30,-0.0 -1,-0.2 -0.704 50.5-143.0 -86.8 119.1 2.4 16.5 12.9 19 22 A N E +A 14 0A 97 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.400 26.9 167.1 -77.4 152.7 -0.5 14.3 14.0 20 23 A F E -A 13 0A 3 -7,-2.1 -7,-2.4 -2,-0.1 2,-0.4 -0.972 25.4-153.7-164.8 148.0 -3.2 13.3 11.6 21 24 A V E -AC 12 43A 6 22,-1.9 21,-2.6 -2,-0.3 22,-1.6 -0.992 13.0-177.5-127.4 139.9 -6.2 11.0 11.0 22 25 A I E -AC 11 41A 0 -11,-2.5 -11,-3.9 -2,-0.4 2,-0.3 -0.995 4.4-168.1-137.4 127.8 -7.5 9.9 7.6 23 26 A E E -AC 10 40A 104 17,-2.4 17,-3.3 -2,-0.4 2,-0.3 -0.816 11.3-176.9-109.1 154.4 -10.5 7.7 7.0 24 27 A G E -AC 9 39A 8 -15,-1.9 -15,-1.9 -2,-0.3 2,-0.3 -0.989 14.2-172.6-146.6 158.8 -11.4 6.2 3.7 25 28 A E E +AC 8 38A 134 13,-2.0 13,-3.2 -2,-0.3 2,-0.3 -0.987 25.4 125.1-145.1 150.5 -14.0 4.1 2.0 26 29 A G E -AC 7 37A 13 -19,-2.2 -19,-3.1 -2,-0.3 2,-0.3 -0.949 39.8-106.9-174.5-163.3 -14.1 2.5 -1.4 27 30 A K E +AC 6 36A 115 9,-1.8 9,-2.7 -2,-0.3 2,-0.3 -0.983 24.8 176.2-147.0 153.5 -14.6 -0.6 -3.6 28 31 A G E -AC 5 35A 4 -23,-2.4 -23,-2.6 -2,-0.3 7,-0.3 -0.988 34.2-120.1-159.2 170.7 -12.5 -2.9 -5.8 29 32 A N E >>> - C 0 34A 63 5,-1.9 5,-1.5 -2,-0.3 4,-1.2 -0.890 24.3-168.3-117.9 93.2 -12.4 -6.0 -7.9 30 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.8 0, 0.0 -1,-0.2 0.814 87.0 50.1 -53.4 -39.3 -9.9 -8.3 -6.2 31 34 A Y T 345S+ 0 0 104 -28,-0.3 35,-0.1 1,-0.2 -27,-0.1 0.709 113.7 45.5 -80.3 -17.7 -9.7 -10.7 -9.2 32 35 A E T <45S- 0 0 110 -3,-1.1 33,-0.3 -29,-0.3 -1,-0.2 0.530 108.2-124.8 -95.2 -11.4 -9.1 -7.8 -11.6 33 36 A G T <5 + 0 0 0 -4,-1.2 175,-2.1 31,-0.3 2,-0.3 0.728 68.4 117.8 78.7 25.0 -6.5 -6.1 -9.3 34 37 A T E < +CD 29 207A 23 -5,-1.5 -5,-1.9 173,-0.2 2,-0.3 -0.859 31.4 164.4-129.8 156.2 -8.2 -2.7 -9.2 35 38 A Q E -CD 28 206A 10 171,-1.2 171,-1.6 -2,-0.3 2,-0.4 -0.958 21.4-149.0-167.9 146.0 -9.7 -0.5 -6.5 36 39 A I E -CD 27 205A 51 -9,-2.7 -9,-1.8 -2,-0.3 2,-0.4 -0.991 15.7-171.8-122.3 136.8 -10.8 3.0 -5.8 37 40 A L E -CD 26 204A 1 167,-1.8 167,-2.0 -2,-0.4 2,-0.5 -0.983 15.3-150.2-125.9 134.5 -10.7 4.6 -2.4 38 41 A D E -CD 25 203A 58 -13,-3.2 -13,-2.0 -2,-0.4 2,-0.4 -0.934 23.6-161.5-101.2 124.6 -12.1 8.0 -1.3 39 42 A L E -CD 24 202A 1 163,-3.6 163,-2.1 -2,-0.5 2,-0.5 -0.926 10.4-171.0-116.9 134.3 -9.9 9.4 1.5 40 43 A N E -CD 23 201A 47 -17,-3.3 -17,-2.4 -2,-0.4 2,-0.4 -0.966 20.3-137.3-128.0 119.7 -10.8 12.1 4.0 41 44 A V E +C 22 0A 19 159,-2.2 -19,-0.3 -2,-0.5 3,-0.1 -0.575 26.0 172.6 -70.2 124.9 -8.5 13.8 6.4 42 45 A T E + 0 0 60 -21,-2.6 2,-0.3 -2,-0.4 -20,-0.2 0.462 64.5 27.2-114.0 -5.0 -10.4 14.0 9.7 43 46 A E E S+C 21 0A 105 -22,-1.6 -22,-1.9 1,-0.0 -1,-0.3 -0.963 113.4 25.3-151.4 138.9 -7.6 15.4 12.0 44 47 A G S S- 0 0 28 -2,-0.3 -22,-0.0 1,-0.3 -3,-0.0 0.598 84.3-164.0 90.5 9.3 -4.5 17.4 11.3 45 48 A A + 0 0 51 1,-0.2 -1,-0.3 2,-0.1 154,-0.1 -0.468 63.1 45.0 -75.4 145.4 -5.7 19.2 8.2 46 49 A P S S- 0 0 70 0, 0.0 -1,-0.2 0, 0.0 153,-0.1 0.444 86.8-137.6 -87.0 134.9 -4.2 20.7 6.1 47 50 A L - 0 0 3 151,-0.4 2,-0.2 1,-0.2 -2,-0.1 -0.247 6.5-152.8 -52.7 128.3 -1.4 18.1 5.8 48 51 A P + 0 0 54 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 -0.147 68.7 63.0 -99.3 45.7 1.8 19.9 5.6 49 52 A F S S- 0 0 4 -2,-0.2 78,-0.2 79,-0.1 2,-0.2 -0.984 98.2 -71.1-157.0 163.1 3.6 17.2 3.6 50 53 A A > - 0 0 3 76,-1.9 3,-1.3 -2,-0.3 4,-0.2 -0.370 34.5-149.1 -65.7 125.3 3.4 15.4 0.2 51 54 A Y T >> S+ 0 0 8 1,-0.2 3,-2.6 -2,-0.2 4,-0.6 0.810 88.2 77.5 -63.7 -32.0 0.3 13.1 0.2 52 55 A D H >> S+ 0 0 8 1,-0.3 3,-0.9 2,-0.2 4,-0.5 0.804 79.8 71.1 -49.3 -29.0 2.1 10.6 -2.1 53 56 A I H <4 S+ 0 0 0 -3,-1.3 -1,-0.3 1,-0.2 -2,-0.2 0.724 104.7 41.0 -62.9 -15.8 4.1 9.3 0.9 54 57 A L H X> S+ 0 0 0 -3,-2.6 4,-1.6 -4,-0.2 3,-1.4 0.602 90.0 88.3-100.5 -21.2 0.8 7.7 2.1 55 58 A T H << S+ 0 0 3 -3,-0.9 -2,-0.1 -4,-0.6 -1,-0.1 0.844 87.6 46.9 -64.4 -40.2 -0.8 6.3 -1.0 56 59 A T T 3< S+ 0 0 28 -4,-0.5 -1,-0.3 2,-0.2 -2,-0.1 0.615 108.7 58.1 -75.4 -10.0 0.8 2.9 -1.1 57 60 A V T <4 0 0 2 -3,-1.4 -2,-0.2 1,-0.1 -1,-0.2 0.849 360.0 360.0 -87.9 -34.2 -0.1 2.4 2.6 58 61 A F < 0 0 8 -4,-1.6 -2,-0.2 -3,-0.1 -3,-0.1 0.911 360.0 360.0 -71.7 360.0 -3.8 3.0 1.7 59 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 60 65 A N > 0 0 10 0, 0.0 3,-2.5 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 92.4 -4.0 -4.5 0.4 61 66 A R T 3 + 0 0 11 1,-0.3 14,-0.2 13,-0.0 13,-0.2 0.388 360.0 87.9 -62.7 1.2 -2.4 -7.1 -1.9 62 67 A A T 3 S+ 0 0 0 1,-0.2 2,-1.7 12,-0.2 -1,-0.3 0.829 77.5 70.5 -62.3 -32.4 -5.8 -8.9 -1.9 63 68 A F S < S+ 0 0 0 -3,-2.5 2,-0.3 -30,-0.1 -1,-0.2 -0.585 77.0 110.7 -85.2 75.6 -6.4 -6.5 -4.8 64 69 A T - 0 0 0 -2,-1.7 2,-0.7 -34,-0.8 -31,-0.3 -0.986 68.3-126.6-145.9 138.9 -4.1 -8.2 -7.2 65 70 A K B -e 209 0A 101 143,-2.2 145,-3.0 -2,-0.3 -34,-0.1 -0.829 32.5-164.5 -89.9 121.3 -4.9 -10.2 -10.3 66 71 A Y - 0 0 32 -2,-0.7 145,-0.1 143,-0.2 6,-0.1 -0.953 18.7-122.4-112.3 123.5 -3.1 -13.5 -9.9 67 72 A P > - 0 0 49 0, 0.0 3,-1.1 0, 0.0 144,-0.0 -0.083 35.0-108.1 -48.1 159.3 -2.5 -16.1 -12.8 68 73 A A T 3 S+ 0 0 115 1,-0.2 4,-0.1 3,-0.1 -2,-0.0 0.795 117.4 50.8 -64.1 -28.3 -4.0 -19.5 -12.0 69 74 A D T 3 S+ 0 0 136 2,-0.1 2,-0.5 3,-0.0 -1,-0.2 0.176 90.7 83.4-102.3 9.3 -0.6 -21.0 -11.5 70 75 A I S < S- 0 0 32 -3,-1.1 2,-0.1 140,-0.1 140,-0.1 -0.983 88.8-116.1-112.8 120.8 0.8 -18.4 -9.0 71 76 A Q - 0 0 50 -2,-0.5 2,-1.2 1,-0.1 -2,-0.1 -0.356 21.3-143.7 -56.7 121.2 -0.3 -19.2 -5.5 72 77 A D > + 0 0 18 1,-0.2 4,-1.2 -4,-0.1 -1,-0.1 -0.689 25.9 172.8 -92.4 82.2 -2.5 -16.3 -4.3 73 78 A Y T 4 S+ 0 0 5 -2,-1.2 4,-0.3 1,-0.2 -1,-0.2 0.876 71.3 55.6 -51.2 -45.2 -1.4 -16.0 -0.6 74 79 A F T >4 S+ 0 0 0 1,-0.2 3,-1.6 2,-0.2 4,-0.3 0.925 106.1 45.5 -67.9 -49.1 -3.4 -12.9 0.1 75 80 A K G >4 S+ 0 0 19 1,-0.3 3,-1.0 -14,-0.2 -1,-0.2 0.820 110.3 59.9 -59.7 -29.7 -6.9 -13.9 -1.0 76 81 A Q G 3< S+ 0 0 92 -4,-1.2 -1,-0.3 1,-0.2 -2,-0.2 0.486 86.7 72.2 -80.9 -2.8 -6.3 -17.1 1.0 77 82 A T G <> S+ 0 0 1 -3,-1.6 4,-3.2 -4,-0.3 99,-0.3 0.567 87.3 133.4 -85.8 -8.3 -5.7 -15.4 4.4 78 83 A F T <4 + 0 0 21 -3,-1.0 25,-0.0 -4,-0.3 96,-0.0 -0.660 47.4 26.1 -94.1 153.1 -9.4 -14.6 4.5 79 84 A P T 4 S+ 0 0 93 0, 0.0 25,-0.4 0, 0.0 -1,-0.2 -0.928 131.9 41.7 -76.0 -21.0 -11.8 -14.7 6.2 80 85 A E T 4 S- 0 0 60 1,-0.2 -2,-0.3 23,-0.1 2,-0.2 0.844 100.7-153.4 -60.0 -40.6 -9.4 -14.4 9.2 81 86 A G < - 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