==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=12-OCT-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSPORT PROTEIN 26-JAN-12 4AGE . COMPND 2 MOLECULE: SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR C.PLIOTAS,E.BRANNIGAN,J.H.NAISMITH . 1792 7 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 87443.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1473 82.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 42 2.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 315 17.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 7 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 21 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 153 8.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 155 8.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 763 42.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 14 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 8 0 0 0 0 0 0 0 7 7 0 7 0 0 7 0 0 0 0 0 7 0 0 0 0 7 RESIDUES PER ALPHA HELIX . 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 7 7 14 7 7 0 7 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 7 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 25 A L > 0 0 180 0, 0.0 3,-1.4 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 48.8 -59.5 -18.9 82.3 2 26 A S T 3 + 0 0 113 1,-0.3 3,-0.5 2,-0.1 4,-0.1 0.512 360.0 66.7 -77.9 -7.2 -60.6 -15.3 82.6 3 27 A Y T 3 S+ 0 0 193 1,-0.2 -1,-0.3 2,-0.1 3,-0.2 0.303 83.4 75.3 -91.6 5.3 -56.9 -14.4 82.4 4 28 A A S < S+ 0 0 52 -3,-1.4 4,-0.5 1,-0.2 -1,-0.2 0.135 75.8 78.5 -94.4 12.7 -56.9 -15.7 78.8 5 29 A V S > S+ 0 0 93 -3,-0.5 4,-2.0 2,-0.2 3,-0.2 0.700 80.7 66.8 -84.7 -28.2 -58.7 -12.4 77.9 6 30 A N H > S+ 0 0 62 1,-0.2 4,-2.4 -3,-0.2 -2,-0.1 0.779 92.5 60.8 -61.1 -30.1 -55.4 -10.6 78.0 7 31 A I H > S+ 0 0 97 2,-0.2 4,-1.6 1,-0.2 -1,-0.2 0.928 106.7 44.8 -61.0 -46.2 -54.3 -12.7 74.9 8 32 A V H > S+ 0 0 97 -4,-0.5 4,-2.0 1,-0.2 -2,-0.2 0.893 113.1 51.1 -65.2 -40.4 -57.2 -11.1 73.0 9 33 A A H X S+ 0 0 45 -4,-2.0 4,-2.2 1,-0.2 -2,-0.2 0.863 104.8 58.0 -64.0 -37.6 -56.3 -7.6 74.3 10 34 A A H X S+ 0 0 12 -4,-2.4 4,-1.7 2,-0.2 -1,-0.2 0.915 107.2 46.7 -57.7 -47.4 -52.6 -8.2 73.3 11 35 A L H X S+ 0 0 105 -4,-1.6 4,-2.6 2,-0.2 5,-0.3 0.950 108.6 55.4 -59.5 -51.7 -53.7 -8.7 69.7 12 36 A A H X S+ 0 0 51 -4,-2.0 4,-2.6 1,-0.3 5,-0.3 0.902 108.2 48.5 -48.8 -49.1 -56.0 -5.6 69.8 13 37 A I H X S+ 0 0 58 -4,-2.2 4,-2.5 1,-0.2 -1,-0.3 0.877 111.3 50.8 -60.6 -40.4 -53.1 -3.4 70.9 14 38 A I H X S+ 0 0 56 -4,-1.7 4,-3.2 -3,-0.2 -2,-0.2 0.928 113.4 43.0 -65.0 -47.9 -50.8 -4.9 68.1 15 39 A I H X S+ 0 0 105 -4,-2.6 4,-2.3 2,-0.2 5,-0.2 0.953 119.0 43.8 -64.4 -50.8 -53.3 -4.3 65.3 16 40 A V H X S+ 0 0 92 -4,-2.6 4,-2.9 -5,-0.3 -1,-0.2 0.891 116.8 49.2 -57.4 -42.8 -54.3 -0.8 66.6 17 41 A G H X S+ 0 0 3 -4,-2.5 4,-2.4 -5,-0.3 -2,-0.2 0.908 108.8 50.3 -64.5 -47.4 -50.6 -0.1 67.1 18 42 A L H X S+ 0 0 74 -4,-3.2 4,-2.6 2,-0.2 -2,-0.2 0.917 116.9 42.3 -57.7 -45.1 -49.5 -1.3 63.7 19 43 A I H X S+ 0 0 106 -4,-2.3 4,-2.0 2,-0.2 5,-0.2 0.928 113.6 49.9 -68.1 -48.4 -52.1 0.9 62.1 20 44 A I H X S+ 0 0 89 -4,-2.9 4,-1.5 1,-0.2 -2,-0.2 0.864 115.6 45.8 -59.4 -37.2 -51.5 4.0 64.4 21 45 A A H X S+ 0 0 0 -4,-2.4 4,-2.3 -5,-0.2 5,-0.3 0.936 110.8 51.1 -68.9 -51.5 -47.8 3.6 63.6 22 46 A R H X S+ 0 0 150 -4,-2.6 4,-1.4 1,-0.2 -2,-0.2 0.786 114.0 45.1 -58.0 -30.7 -48.3 3.2 59.8 23 47 A M H X S+ 0 0 131 -4,-2.0 4,-1.4 2,-0.2 -1,-0.2 0.753 111.3 49.8 -92.2 -26.5 -50.5 6.3 59.6 24 48 A I H X S+ 0 0 74 -4,-1.5 4,-0.8 -5,-0.2 -2,-0.2 0.959 113.6 47.1 -67.2 -52.6 -48.4 8.6 61.8 25 49 A S H >X S+ 0 0 5 -4,-2.3 4,-2.1 1,-0.3 3,-1.1 0.914 112.8 47.5 -58.8 -49.6 -45.2 7.7 59.8 26 50 A N H 3X S+ 0 0 118 -4,-1.4 4,-0.9 -5,-0.3 -1,-0.3 0.950 113.4 50.8 -49.9 -49.3 -47.0 8.2 56.4 27 51 A A H 3< S+ 0 0 65 -4,-1.4 4,-0.4 1,-0.2 -2,-0.2 0.519 108.3 52.8 -75.8 -6.1 -48.3 11.5 57.8 28 52 A V H S+ 0 0 91 -3,-0.4 4,-0.9 -4,-0.4 -2,-0.2 0.906 114.2 48.5 -61.9 -47.9 -44.9 16.7 55.9 32 56 A M H >X S+ 0 0 46 -4,-2.0 4,-2.0 1,-0.2 3,-1.0 0.935 111.1 48.6 -61.6 -49.5 -41.2 16.4 55.2 33 57 A I H 3< S+ 0 0 95 -4,-1.1 -1,-0.2 1,-0.3 -2,-0.2 0.842 107.3 57.5 -60.9 -34.3 -41.6 15.9 51.4 34 58 A S H 3< S+ 0 0 69 -4,-1.6 -1,-0.3 1,-0.1 -2,-0.2 0.710 107.9 47.3 -71.4 -23.2 -43.9 19.0 51.3 35 59 A R H << S- 0 0 186 -3,-1.0 -2,-0.2 -4,-0.9 -1,-0.1 0.957 116.5-109.9 -74.9 -63.4 -41.1 21.1 52.8 36 60 A K < + 0 0 181 -4,-2.0 3,-0.2 2,-0.0 -3,-0.1 -0.047 67.8 138.5 163.5 -36.3 -38.3 20.0 50.5 37 61 A I S S- 0 0 74 -5,-0.3 2,-0.1 1,-0.1 -4,-0.1 0.340 70.9 -77.1 -9.4 -75.3 -35.9 17.9 52.7 38 62 A D > - 0 0 41 1,-0.1 4,-2.4 2,-0.0 5,-0.2 -0.433 28.8-116.0 158.4 122.1 -35.1 14.9 50.3 39 63 A A H > S+ 0 0 66 1,-0.2 4,-2.0 2,-0.2 5,-0.1 0.775 113.3 43.1 -41.5 -44.2 -37.1 11.8 49.3 40 64 A T H > S+ 0 0 62 2,-0.2 4,-2.8 1,-0.2 -1,-0.2 0.945 112.0 49.8 -71.7 -51.8 -34.6 9.3 50.9 41 65 A V H > S+ 0 0 53 1,-0.2 4,-2.5 2,-0.2 5,-0.3 0.862 112.2 52.4 -57.4 -35.8 -33.9 11.2 54.1 42 66 A A H X S+ 0 0 0 -4,-2.4 4,-3.3 2,-0.2 5,-0.3 0.979 109.6 46.1 -60.5 -59.4 -37.7 11.4 54.5 43 67 A C H X S+ 0 0 73 -4,-2.0 4,-2.6 -5,-0.2 -2,-0.2 0.867 117.5 45.7 -53.3 -42.3 -38.3 7.7 54.1 44 68 A F H X S+ 0 0 54 -4,-2.8 4,-1.1 2,-0.2 -2,-0.2 0.992 115.3 42.3 -66.0 -65.9 -35.5 6.9 56.4 45 69 A L H >X S+ 0 0 71 -4,-2.5 4,-1.1 1,-0.2 3,-0.6 0.882 118.4 50.6 -46.8 -43.5 -36.3 9.4 59.3 46 70 A S H >X S+ 0 0 9 -4,-3.3 4,-2.2 -5,-0.3 3,-0.9 0.949 104.1 55.0 -59.4 -53.2 -39.9 8.4 58.7 47 71 A A H 3X S+ 0 0 30 -4,-2.6 4,-1.8 1,-0.3 -1,-0.2 0.603 102.6 59.5 -64.2 -13.1 -39.3 4.6 58.9 48 72 A L H X S+ 0 0 10 -4,-1.5 4,-1.8 2,-0.2 3,-0.5 0.926 106.2 47.9 -73.7 -48.8 -40.3 3.1 67.0 53 77 A I H 3X S+ 0 0 29 -4,-3.2 4,-2.1 1,-0.3 -2,-0.2 0.881 112.1 48.7 -64.4 -38.2 -44.0 3.3 67.5 54 78 A I H 3X S+ 0 0 44 -4,-1.9 4,-1.7 -5,-0.3 -1,-0.3 0.662 106.2 60.0 -77.2 -14.6 -44.4 -0.5 66.9 55 79 A A H <> S+ 0 0 15 -3,-0.5 4,-1.4 -4,-0.4 -2,-0.2 0.860 107.6 43.8 -72.3 -42.3 -41.6 -0.9 69.4 56 80 A F H X S+ 0 0 33 -4,-1.8 4,-2.1 2,-0.2 5,-0.3 0.900 112.7 53.0 -64.3 -44.1 -43.7 0.8 72.0 57 81 A T H X S+ 0 0 20 -4,-2.1 4,-2.5 1,-0.2 5,-0.2 0.913 107.9 50.3 -59.2 -45.1 -46.7 -1.2 70.9 58 82 A L H X S+ 0 0 56 -4,-1.7 4,-2.0 1,-0.2 5,-0.3 0.844 109.5 51.9 -63.5 -35.2 -44.8 -4.5 71.3 59 83 A I H X S+ 0 0 13 -4,-1.4 4,-1.7 2,-0.2 10,-0.2 0.929 117.1 36.5 -66.1 -48.1 -43.7 -3.5 74.9 60 84 A A H X S+ 0 0 33 -4,-2.1 4,-0.9 2,-0.2 -2,-0.2 0.805 117.8 52.1 -75.9 -32.4 -47.2 -2.6 76.1 61 85 A A H >< S+ 0 0 1 -4,-2.5 3,-0.6 -5,-0.3 4,-0.3 0.935 115.4 38.8 -72.6 -49.9 -48.8 -5.5 74.2 62 86 A L H ><>S+ 0 0 49 -4,-2.0 3,-2.4 -5,-0.2 5,-0.6 0.903 109.4 66.4 -63.0 -41.2 -46.5 -8.2 75.5 63 87 A G H ><5S+ 0 0 34 -4,-1.7 3,-1.0 -5,-0.3 -1,-0.2 0.737 93.4 56.9 -50.4 -32.0 -46.6 -6.4 78.8 64 88 A R T <<5S+ 0 0 150 -4,-0.9 -1,-0.3 -3,-0.6 -2,-0.2 0.569 104.6 54.2 -79.9 -10.2 -50.4 -7.3 79.2 65 89 A V T < 5S- 0 0 69 -3,-2.4 -1,-0.3 -4,-0.3 -2,-0.2 -0.164 128.8 -99.2-108.1 34.5 -49.3 -10.9 78.8 66 90 A G T < 5 + 0 0 54 -3,-1.0 2,-0.2 1,-0.2 -3,-0.2 0.882 68.6 152.9 54.6 51.7 -46.8 -10.5 81.7 67 91 A V < - 0 0 23 -5,-0.6 2,-2.1 -8,-0.2 4,-0.4 -0.478 56.6-120.5-110.2 54.4 -43.6 -10.0 79.7 68 92 A Q - 0 0 110 -2,-0.2 2,-3.3 1,-0.2 -5,-0.1 0.293 24.7-129.2 35.2 9.6 -41.8 -8.0 82.3 69 93 A T S >> S+ 0 0 41 -2,-2.1 4,-1.9 1,-0.3 3,-1.1 -0.172 103.1 64.7 51.0 -63.8 -41.2 -4.8 80.1 70 94 A A T 34 S+ 0 0 55 -2,-3.3 -1,-0.3 1,-0.3 -2,-0.1 0.342 98.0 56.8 -72.5 7.6 -37.5 -4.6 80.8 71 95 A S T 3> S+ 0 0 1 -4,-0.4 4,-2.9 2,-0.1 -1,-0.3 0.620 103.4 52.2-102.5 -26.8 -37.1 -8.0 79.0 72 96 A V H <> S+ 0 0 0 -3,-1.1 4,-2.5 -5,-0.4 5,-0.5 0.924 106.7 51.7 -72.7 -45.5 -38.7 -6.6 75.8 73 97 A I H X S+ 0 0 69 -4,-1.9 4,-1.3 1,-0.2 -1,-0.2 0.892 115.8 45.9 -54.2 -35.4 -36.3 -3.6 75.8 74 98 A A H > S+ 0 0 37 -5,-0.2 4,-2.2 2,-0.2 -2,-0.2 0.957 114.3 42.6 -76.5 -53.8 -33.6 -6.3 76.1 75 99 A V H X S+ 0 0 25 -4,-2.9 4,-3.0 2,-0.2 -2,-0.2 0.962 118.6 45.1 -57.8 -54.3 -34.7 -8.8 73.5 76 100 A L H X S+ 0 0 63 -4,-2.5 4,-3.2 1,-0.2 5,-0.3 0.872 109.4 57.9 -59.2 -37.4 -35.6 -6.1 70.9 77 101 A G H X S+ 0 0 44 -4,-1.3 4,-2.4 -5,-0.5 -1,-0.2 0.940 110.2 43.0 -53.3 -49.8 -32.3 -4.4 71.7 78 102 A A H X S+ 0 0 59 -4,-2.2 4,-3.1 2,-0.2 -2,-0.2 0.957 113.5 50.8 -63.8 -50.4 -30.5 -7.6 70.7 79 103 A A H X S+ 0 0 27 -4,-3.0 4,-2.5 1,-0.2 5,-0.2 0.954 112.9 46.1 -50.9 -57.2 -32.7 -8.2 67.7 80 104 A G H X S+ 0 0 41 -4,-3.2 4,-3.7 1,-0.2 -1,-0.2 0.903 111.4 53.0 -51.1 -47.4 -32.0 -4.6 66.5 81 105 A L H X S+ 0 0 111 -4,-2.4 4,-2.1 -5,-0.3 -1,-0.2 0.938 108.2 49.5 -56.7 -47.4 -28.3 -5.1 67.2 82 106 A A H X S+ 0 0 33 -4,-3.1 4,-1.9 2,-0.2 -2,-0.2 0.928 115.4 43.7 -62.5 -44.9 -28.2 -8.2 65.1 83 107 A V H X S+ 0 0 46 -4,-2.5 4,-2.7 -5,-0.2 -2,-0.2 0.981 108.7 59.7 -56.0 -56.3 -29.9 -6.5 62.2 84 108 A G H X S+ 0 0 36 -4,-3.7 4,-1.4 1,-0.2 -2,-0.2 0.834 108.3 43.9 -41.4 -46.8 -27.8 -3.4 62.7 85 109 A L H < S+ 0 0 85 -4,-2.1 4,-0.5 1,-0.2 -1,-0.2 0.975 111.5 51.9 -66.2 -54.9 -24.6 -5.4 62.1 86 110 A A H < S+ 0 0 15 -4,-1.9 4,-0.2 -5,-0.2 -2,-0.2 0.844 121.4 33.4 -52.1 -40.4 -25.9 -7.3 59.1 87 111 A L H >< S+ 0 0 99 -4,-2.7 3,-2.5 -5,-0.1 2,-0.2 0.973 85.9 96.0 -77.4 -77.1 -27.0 -4.0 57.5 88 112 A Q T 3X S+ 0 0 104 -4,-1.4 4,-3.9 1,-0.3 5,-0.4 0.130 81.4 57.1 17.7 -74.9 -24.5 -1.2 58.4 89 113 A G H 3> S+ 0 0 41 -4,-0.5 4,-2.5 1,-0.2 -1,-0.3 0.920 113.4 45.7 -34.4 -53.5 -22.3 -1.4 55.3 90 114 A S H <> S+ 0 0 12 -3,-2.5 4,-3.5 -4,-0.2 3,-0.3 0.986 110.2 50.7 -51.7 -64.5 -25.5 -0.8 53.5 91 115 A L H > S+ 0 0 38 1,-0.3 4,-2.1 2,-0.2 -2,-0.2 0.898 112.7 48.3 -45.0 -47.2 -26.6 2.0 55.9 92 116 A S H X S+ 0 0 63 -4,-3.9 4,-2.0 1,-0.2 -1,-0.3 0.897 114.3 45.0 -60.4 -45.8 -23.2 3.6 55.3 93 117 A N H X S+ 0 0 26 -4,-2.5 4,-2.2 -5,-0.4 -1,-0.2 0.851 108.9 56.8 -65.1 -38.3 -23.5 3.2 51.6 94 118 A L H X S+ 0 0 48 -4,-3.5 4,-1.9 1,-0.2 -2,-0.2 0.916 108.9 47.2 -59.9 -42.7 -27.1 4.5 51.6 95 119 A A H X S+ 0 0 20 -4,-2.1 4,-2.1 -5,-0.3 -2,-0.2 0.896 108.9 51.8 -67.9 -45.1 -26.0 7.7 53.3 96 120 A A H X S+ 0 0 2 -4,-2.0 4,-3.0 1,-0.2 -1,-0.2 0.914 105.3 60.3 -57.2 -40.1 -23.1 8.2 50.9 97 121 A G H X S+ 0 0 6 -4,-2.2 4,-2.2 29,-0.4 5,-0.3 0.962 105.8 45.0 -48.9 -61.3 -25.7 7.8 48.2 98 122 A V H >X S+ 0 0 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S+ 0 0 69 -2,-0.3 14,-0.2 1,-0.2 3,-0.1 -0.269 120.3 29.3 -49.6 128.8 -24.0 11.4 35.6 109 133 A G T 3 S+ 0 0 51 12,-1.8 -1,-0.2 1,-0.4 2,-0.2 0.227 88.7 118.0 102.5 -14.1 -21.9 11.8 32.4 110 134 A E < - 0 0 17 -3,-2.1 11,-2.6 10,-0.1 2,-0.5 -0.614 65.6-125.0 -82.6 146.2 -18.8 13.3 33.9 111 135 A Y E +A 120 0A 77 9,-0.2 43,-2.1 -2,-0.2 44,-0.7 -0.831 43.6 168.1 -90.8 127.6 -15.5 11.4 33.6 112 136 A V E -AB 119 153A 0 7,-2.4 7,-1.8 -2,-0.5 2,-0.6 -0.946 38.9-130.6-142.7 155.0 -14.0 10.9 37.0 113 137 A D E -AB 118 152A 34 39,-2.8 39,-1.9 -2,-0.3 2,-0.8 -0.950 19.7-171.7-110.4 113.1 -11.3 9.1 39.0 114 138 A L S S- 0 0 3 3,-2.3 3,-0.4 -2,-0.6 37,-0.1 -0.598 70.9 -47.4-106.6 66.5 -12.8 7.5 42.1 115 139 A G S S- 0 0 47 -2,-0.8 2,-0.5 35,-0.2 -1,-0.2 1.000 122.4 -23.6 71.0 72.5 -9.6 6.3 43.9 116 140 A G S S+ 0 0 68 1,-0.1 2,-0.3 -3,-0.1 -1,-0.2 -0.238 124.4 81.0 94.8 -44.0 -7.5 4.5 41.3 117 141 A V - 0 0 28 -2,-0.5 -3,-2.3 -3,-0.4 2,-0.4 -0.741 58.7-173.6 -94.6 146.1 -10.3 3.6 38.9 118 142 A A 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