==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-NOV-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNOGLOBULIN 15-MAY-97 1AJ7 . COMPND 2 MOLECULE: IMMUNOGLOBULIN 48G7 FAB (LIGHT CHAIN); . SOURCE 2 FRAGMENT: CONSTANT DOMAINS OF LIGHT AND HEAVY CHAINS; . AUTHOR G.J.WEDEMAYER,L.H.WANG,P.A.PATTEN,P.G.SCHULTZ,R.C.STEVENS . 431 2 5 4 1 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19542.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 280 65.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 10 2.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 167 38.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 5 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 49 11.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 35 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 12 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 4 3 5 4 9 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 1 4 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 L D 0 0 93 0, 0.0 2,-0.7 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 143.3 12.9 -6.9 36.9 2 2 L I - 0 0 11 93,-0.4 2,-0.4 91,-0.1 88,-0.1 -0.851 360.0-139.7 -91.8 117.2 9.8 -4.8 36.0 3 3 L Q - 0 0 104 -2,-0.7 23,-1.9 94,-0.0 2,-0.7 -0.637 8.4-155.4 -82.0 131.0 8.8 -5.6 32.4 4 4 L M E -A 25 0A 13 94,-0.5 2,-0.7 -2,-0.4 96,-0.5 -0.940 7.8-163.0-107.5 109.3 7.7 -2.7 30.2 5 5 L T E -A 24 0A 91 19,-2.5 19,-2.4 -2,-0.7 2,-0.4 -0.865 9.3-168.9 -97.3 110.3 5.4 -4.0 27.5 6 6 L Q E -A 23 0A 18 -2,-0.7 17,-0.2 17,-0.2 94,-0.0 -0.846 12.8-129.7-104.4 133.5 5.1 -1.4 24.7 7 7 L S E S+A 22 0A 66 15,-2.1 15,-2.1 -2,-0.4 2,-0.1 -0.989 70.9 23.2-142.4 145.2 2.6 -1.6 21.9 8 8 L P - 0 0 50 0, 0.0 3,-0.1 0, 0.0 -1,-0.1 0.686 60.0-153.3 -84.6 172.3 2.4 -1.4 19.0 9 9 L S S S+ 0 0 90 1,-0.2 94,-2.8 93,-0.2 2,-0.3 0.847 86.7 18.6 -70.7 -36.5 5.9 -2.3 17.8 10 10 L S E -d 103 0B 59 92,-0.2 2,-0.4 75,-0.0 -1,-0.2 -0.990 67.8-171.8-136.8 144.8 5.3 -0.2 14.7 11 11 L L E -d 104 0B 42 92,-2.8 94,-3.4 -2,-0.3 2,-0.5 -0.999 7.1-160.6-140.4 129.9 2.7 2.5 13.9 12 12 L S E +d 105 0B 54 -2,-0.4 2,-0.3 92,-0.2 94,-0.2 -0.964 22.7 169.7-112.3 128.0 2.0 4.1 10.5 13 13 L A E -d 106 0B 6 92,-2.2 94,-2.7 -2,-0.5 2,-0.1 -0.999 31.1-119.5-143.9 146.6 0.2 7.4 10.7 14 14 L S E > -d 107 0B 57 -2,-0.3 3,-1.7 92,-0.2 64,-0.3 -0.392 47.2 -87.2 -78.5 157.0 -0.7 10.3 8.4 15 15 L L T 3 S+ 0 0 77 92,-1.1 64,-0.2 1,-0.2 -1,-0.1 -0.368 115.0 23.8 -64.3 138.6 0.5 13.9 9.0 16 16 L G T 3 S+ 0 0 49 62,-2.8 -1,-0.2 1,-0.3 2,-0.1 0.328 93.4 133.7 87.9 -8.5 -1.8 15.8 11.3 17 17 L E < - 0 0 104 -3,-1.7 61,-2.4 61,-0.2 2,-0.7 -0.410 60.0-122.7 -76.3 151.2 -3.2 12.6 12.9 18 18 L R - 0 0 151 59,-0.2 2,-0.3 -2,-0.1 58,-0.2 -0.860 39.9-180.0 -95.5 113.6 -3.5 12.2 16.6 19 19 L V E - B 0 75A 11 56,-2.0 56,-2.8 -2,-0.7 2,-0.3 -0.853 13.6-164.8-118.7 156.4 -1.5 9.1 17.6 20 20 L S E - B 0 74A 58 -2,-0.3 2,-0.3 54,-0.2 54,-0.2 -0.987 5.1-162.5-141.3 145.3 -0.8 7.3 20.9 21 21 L L E - B 0 73A 0 52,-2.4 52,-2.4 -2,-0.3 2,-0.3 -0.938 16.1-152.9-123.1 148.6 1.7 4.7 22.1 22 22 L T E -AB 7 72A 43 -15,-2.1 -15,-2.1 -2,-0.3 2,-0.4 -0.910 12.5-169.4-129.8 151.3 1.2 2.7 25.3 23 23 L a E -AB 6 71A 0 48,-2.3 48,-2.8 -2,-0.3 2,-0.5 -0.996 9.4-157.4-136.7 129.1 3.4 0.9 27.8 24 24 L R E -AB 5 70A 130 -19,-2.4 -19,-2.5 -2,-0.4 2,-0.3 -0.959 11.3-145.3-112.9 120.8 2.2 -1.5 30.5 25 25 L A E -A 4 0A 3 44,-1.9 -21,-0.2 -2,-0.5 4,-0.1 -0.677 5.6-148.1 -82.7 136.3 4.3 -2.0 33.6 26 26 L S S S+ 0 0 58 -23,-1.9 2,-0.3 -2,-0.3 -1,-0.1 0.661 84.7 35.7 -76.8 -15.5 4.3 -5.5 35.0 27 27 L Q S S- 0 0 102 -24,-0.2 2,-0.4 42,-0.1 -2,-0.1 -0.853 103.7 -81.9-131.0 167.0 4.8 -4.0 38.5 28 28 L E + 0 0 120 -2,-0.3 -2,-0.1 1,-0.2 41,-0.1 -0.573 45.0 170.3 -71.4 120.3 3.7 -0.8 40.3 29 29 L I > - 0 0 0 -2,-0.4 3,-2.5 -4,-0.1 62,-0.2 0.256 36.0-136.6-114.6 6.4 6.1 1.9 39.2 30 30 L S T 3 - 0 0 67 1,-0.3 3,-0.1 2,-0.1 -2,-0.1 0.745 66.8 -52.8 42.3 43.3 4.1 4.8 40.8 31 31 L G T 3 S+ 0 0 12 1,-0.2 2,-2.2 20,-0.1 -1,-0.3 0.412 97.1 135.8 80.5 -2.6 4.4 7.2 37.9 32 32 L Y < + 0 0 123 -3,-2.5 59,-2.4 18,-0.2 60,-0.4 -0.475 36.1 123.2 -80.4 73.4 8.2 7.0 37.6 33 33 L L E -E 90 0B 0 -2,-2.2 18,-2.7 57,-0.2 2,-0.3 -0.997 40.9-164.8-138.6 139.2 8.2 6.7 33.9 34 34 L S E -E 89 0B 1 55,-2.4 55,-2.6 -2,-0.4 2,-0.5 -0.868 14.3-139.3-120.8 153.9 9.9 8.7 31.1 35 35 L W E -EF 88 48B 0 13,-2.1 12,-3.0 -2,-0.3 13,-1.5 -0.976 22.1-168.7-114.6 125.0 9.3 8.8 27.4 36 36 L L E -EF 87 46B 0 51,-3.2 51,-2.4 -2,-0.5 2,-0.5 -0.879 8.5-150.3-114.2 149.0 12.4 9.0 25.2 37 37 L Q E -EF 86 45B 14 8,-3.1 8,-2.0 -2,-0.3 2,-0.6 -0.978 6.5-168.0-123.7 127.4 12.7 9.7 21.5 38 38 L Q E -EF 85 44B 17 47,-2.9 47,-2.6 -2,-0.5 6,-0.2 -0.958 11.5-155.3-117.3 113.2 15.4 8.3 19.2 39 39 L K > - 0 0 47 4,-2.2 3,-2.5 -2,-0.6 45,-0.2 -0.359 37.7 -92.5 -79.0 166.5 15.6 9.9 15.8 40 40 L P T 3 S+ 0 0 46 0, 0.0 -1,-0.1 0, 0.0 44,-0.1 0.783 123.1 62.1 -48.9 -34.4 17.2 8.1 12.7 41 41 L D T 3 S- 0 0 117 2,-0.1 3,-0.1 1,-0.1 -2,-0.0 0.415 121.0-104.1 -76.3 2.2 20.6 9.6 13.4 42 42 L G S < S+ 0 0 13 -3,-2.5 2,-0.3 1,-0.3 -1,-0.1 0.301 71.5 147.4 93.5 -8.7 20.8 7.8 16.8 43 43 L T - 0 0 71 -5,-0.1 -4,-2.2 -4,-0.0 2,-0.4 -0.450 30.1-162.1 -66.9 123.0 20.0 11.0 18.8 44 44 L I E +F 38 0B 11 276,-0.3 2,-0.3 -2,-0.3 -6,-0.2 -0.906 13.3 172.0-109.2 136.4 18.1 10.2 22.0 45 45 L K E -F 37 0B 100 -8,-2.0 -8,-3.1 -2,-0.4 2,-0.5 -0.994 31.6-120.2-143.9 143.9 16.2 12.9 23.9 46 46 L R E +F 36 0B 10 271,-0.4 -10,-0.2 -2,-0.3 3,-0.1 -0.756 28.2 172.9 -88.2 129.7 13.8 12.8 26.9 47 47 L L E + 0 0 4 -12,-3.0 8,-2.4 -2,-0.5 2,-0.3 0.796 65.0 16.1-101.0 -40.5 10.3 14.2 26.1 48 48 L I E +FG 35 54B 0 -13,-1.5 -13,-2.1 6,-0.2 -1,-0.3 -0.957 56.8 177.4-139.1 152.5 8.4 13.3 29.3 49 49 L Y E > + G 0 53B 60 4,-2.2 4,-2.4 -2,-0.3 3,-0.4 -0.917 67.9 37.9-144.6 167.2 9.1 12.2 32.9 50 50 L A T 4 S- 0 0 37 -2,-0.3 2,-1.8 1,-0.3 -18,-0.2 0.834 122.2 -81.7 55.0 29.8 7.0 11.5 36.0 51 51 L A T 4 S+ 0 0 4 -18,-2.7 -1,-0.3 1,-0.2 21,-0.1 -0.152 124.7 26.6 72.0 -41.2 4.6 10.0 33.5 52 52 L S T 4 S+ 0 0 72 -2,-1.8 2,-0.6 -3,-0.4 12,-0.3 0.339 87.4 109.7-134.8 8.4 3.0 13.3 32.4 53 53 L T E < -G 49 0B 57 -4,-2.4 -4,-2.2 10,-0.1 2,-0.2 -0.794 58.6-140.6 -93.9 118.5 5.5 16.1 32.9 54 54 L L E -G 48 0B 57 -2,-0.6 -6,-0.2 -6,-0.2 3,-0.1 -0.542 19.3-119.3 -76.1 139.6 6.8 17.6 29.7 55 55 L D > - 0 0 22 -8,-2.4 3,-1.8 -2,-0.2 -1,-0.1 -0.335 44.8 -77.9 -73.1 160.7 10.5 18.5 29.6 56 56 L S T 3 S+ 0 0 123 1,-0.3 -1,-0.1 -2,-0.0 0, 0.0 -0.367 117.9 12.6 -61.1 132.9 11.5 22.1 29.0 57 57 L G T 3 S+ 0 0 75 1,-0.3 -1,-0.3 -3,-0.1 3,-0.1 0.358 88.3 139.2 83.1 -6.8 11.2 23.0 25.3 58 58 L V < - 0 0 15 -3,-1.8 -1,-0.3 -11,-0.1 -4,-0.1 -0.552 62.8-103.2 -73.4 130.7 9.3 19.9 24.4 59 59 L P > - 0 0 48 0, 0.0 3,-2.1 0, 0.0 -1,-0.1 -0.285 22.5-122.5 -55.7 139.2 6.5 20.8 21.9 60 60 L K T 3 S+ 0 0 195 1,-0.3 16,-0.1 -3,-0.1 -2,-0.1 0.635 103.7 78.1 -57.2 -18.7 3.0 21.0 23.4 61 61 L R T 3 S+ 0 0 44 14,-0.1 15,-2.3 16,-0.1 2,-0.3 0.709 82.9 78.6 -66.8 -18.7 1.8 18.4 20.9 62 62 L F E < +C 75 0A 5 -3,-2.1 2,-0.3 13,-0.2 13,-0.2 -0.714 59.1 161.8 -91.4 139.6 3.4 15.7 23.1 63 63 L S E -C 74 0A 45 11,-1.6 11,-3.2 -2,-0.3 2,-0.3 -0.982 22.5-150.2-156.2 147.6 1.6 14.6 26.3 64 64 L G E +C 73 0A 12 -12,-0.3 2,-0.3 -2,-0.3 9,-0.2 -0.900 15.3 178.3-121.4 151.7 1.8 11.7 28.6 65 65 L S E -C 72 0A 57 7,-2.1 7,-2.1 -2,-0.3 2,-0.3 -0.947 14.0-146.6-146.8 164.1 -0.9 10.1 30.8 66 66 L R E +C 71 0A 107 -2,-0.3 2,-0.2 5,-0.2 5,-0.2 -0.990 14.9 174.4-136.4 145.1 -1.4 7.2 33.2 67 67 L S E > S-C 70 0A 76 3,-2.4 3,-2.0 -2,-0.3 2,-0.2 -0.769 71.2 -61.4-148.4 91.1 -4.3 4.9 34.0 68 68 L G T 3 S- 0 0 45 1,-0.3 -1,-0.2 -2,-0.2 0, 0.0 -0.452 121.2 -11.5 67.5-126.2 -3.2 2.3 36.4 69 69 L S T 3 S+ 0 0 37 -2,-0.2 -44,-1.9 -3,-0.1 2,-0.6 0.389 118.1 96.9 -87.0 2.8 -0.4 0.2 34.9 70 70 L D E < -BC 24 67A 54 -3,-2.0 -3,-2.4 -46,-0.2 2,-0.5 -0.848 51.5-176.6 -98.8 122.2 -1.1 1.8 31.4 71 71 L Y E -BC 23 66A 2 -48,-2.8 -48,-2.3 -2,-0.6 2,-0.3 -0.982 7.8-168.6-118.4 127.8 1.2 4.7 30.5 72 72 L S E -BC 22 65A 23 -7,-2.1 -7,-2.1 -2,-0.5 2,-0.4 -0.885 17.9-154.9-123.2 150.5 0.5 6.5 27.2 73 73 L L E -BC 21 64A 0 -52,-2.4 -52,-2.4 -2,-0.3 2,-0.4 -0.978 20.2-164.6-117.2 130.9 2.3 9.0 25.0 74 74 L T E -BC 20 63A 12 -11,-3.2 -11,-1.6 -2,-0.4 2,-0.5 -0.965 13.8-164.1-124.8 136.5 0.1 11.1 22.8 75 75 L I E -BC 19 62A 0 -56,-2.8 -56,-2.0 -2,-0.4 3,-0.3 -0.982 8.4-159.7-118.0 113.2 1.1 13.3 19.8 76 76 L S S S+ 0 0 40 -15,-2.3 2,-0.5 -2,-0.5 -1,-0.2 0.938 81.2 13.7 -58.3 -56.4 -1.8 15.7 18.8 77 77 L S S S- 0 0 62 -59,-0.1 -1,-0.3 -58,-0.0 -59,-0.2 -0.899 84.4-133.7-129.0 100.1 -0.8 16.5 15.2 78 78 L L - 0 0 2 -61,-2.4 -62,-2.8 -2,-0.5 2,-0.3 -0.293 26.9-170.4 -55.4 127.9 1.9 14.2 13.8 79 79 L E > - 0 0 87 -64,-0.2 3,-1.5 -63,-0.1 4,-0.1 -0.795 37.0-108.1-118.0 162.1 4.6 16.1 12.0 80 80 L S G > S+ 0 0 18 -2,-0.3 3,-1.6 1,-0.3 90,-0.1 0.828 117.9 62.2 -58.8 -32.9 7.5 14.9 9.8 81 81 L E G 3 S+ 0 0 62 1,-0.3 -1,-0.3 88,-0.2 88,-0.1 0.731 90.0 70.4 -65.9 -21.2 9.9 15.7 12.6 82 82 L D G < + 0 0 2 -3,-1.5 2,-0.6 86,-0.1 -1,-0.3 0.474 64.7 120.9 -77.7 -1.1 8.1 13.1 14.8 83 83 L F < + 0 0 19 -3,-1.6 2,-0.3 85,-0.2 22,-0.1 -0.523 48.6 100.4 -65.3 111.6 9.5 10.2 12.7 84 84 L A E S- H 0 104B 8 20,-0.6 20,-2.7 -2,-0.6 2,-0.5 -0.979 76.1 -86.9-175.4 178.1 11.4 8.2 15.3 85 85 L D E -EH 38 103B 30 -47,-2.6 -47,-2.9 -2,-0.3 2,-0.4 -0.931 40.5-154.5-106.5 126.5 11.4 5.3 17.7 86 86 L Y E -EH 37 102B 0 16,-2.2 16,-1.9 -2,-0.5 2,-0.4 -0.895 10.3-171.6-107.7 134.6 9.9 6.1 21.1 87 87 L Y E -E 36 0B 7 -51,-2.4 -51,-3.2 -2,-0.4 2,-0.3 -0.977 15.3-143.6-126.6 135.5 10.8 4.2 24.3 88 88 L a E -E 35 0B 0 -2,-0.4 11,-2.3 -53,-0.2 2,-0.4 -0.708 19.0-154.9 -92.5 149.8 9.3 4.3 27.8 89 89 L L E -EI 34 98B 20 -55,-2.6 -55,-2.4 -2,-0.3 2,-0.5 -0.996 6.0-148.2-132.9 127.1 11.7 4.0 30.7 90 90 L Q E +EI 33 97B 0 7,-2.7 7,-1.0 -2,-0.4 -57,-0.2 -0.816 22.0 167.4 -93.2 123.3 10.9 2.8 34.2 91 91 L Y + 0 0 32 -59,-2.4 -58,-0.1 -2,-0.5 -1,-0.1 0.006 49.1 98.5-127.6 32.1 13.0 4.4 36.9 92 92 L A S S- 0 0 42 -60,-0.4 2,-0.3 1,-0.2 -1,-0.1 0.807 94.2 -0.1 -85.1 -34.9 11.1 3.3 40.0 93 93 L S S S- 0 0 49 2,-0.2 -1,-0.2 -91,-0.1 4,-0.2 -0.913 90.7 -51.7-148.9 172.3 13.4 0.4 40.9 94 94 L Y S S+ 0 0 71 -2,-0.3 2,-0.1 2,-0.1 -3,-0.1 -0.804 104.0 42.0-101.5 143.7 16.6 -1.4 39.8 95 95 L P S S- 0 0 7 0, 0.0 -93,-0.4 0, 0.0 -2,-0.2 0.531 89.2-129.3 -78.0 155.3 17.4 -2.6 37.2 96 96 L R - 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