==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=18-JAN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSFERASE 23-SEP-12 4BBH . COMPND 2 MOLECULE: GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE; . SOURCE 2 ORGANISM_SCIENTIFIC: PLASMODIUM VIVAX; . AUTHOR M.D.RACKHAM,J.A.BRANNIGAN,D.K.MOSS,Z.YU,A.J.WILKINSON,A.A.HO . 1135 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 53257.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 737 64.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 28 2.5 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 257 22.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 6 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 3 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 7 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 89 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 115 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 216 19.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 17 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 3 1 7 0 0 0 3 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 7 12 8 1 9 6 1 2 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 3 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 27 A D 0 0 163 0, 0.0 2,-0.3 0, 0.0 3,-0.1 0.000 360.0 360.0 360.0 153.8 48.0 40.8 54.5 2 28 A Y > + 0 0 72 1,-0.1 4,-2.7 2,-0.1 5,-0.1 -0.811 360.0 175.4-135.3 83.5 47.1 39.9 58.0 3 29 A K T 4 S+ 0 0 153 -2,-0.3 4,-0.1 2,-0.2 -1,-0.1 0.770 80.4 34.2 -73.9 -32.0 50.3 40.3 59.8 4 30 A F T >4 S+ 0 0 19 2,-0.1 3,-1.9 -3,-0.1 4,-0.4 0.915 117.6 50.4 -84.5 -52.3 48.9 39.6 63.2 5 31 A W G >4 S+ 0 0 31 1,-0.3 3,-1.8 2,-0.2 5,-0.3 0.858 103.9 62.0 -54.1 -38.3 46.2 37.1 62.3 6 32 A Y G 3< S+ 0 0 84 -4,-2.7 -1,-0.3 1,-0.3 -3,-0.1 0.683 96.9 56.8 -65.1 -14.1 48.7 35.0 60.4 7 33 A T G < S+ 0 0 97 -3,-1.9 -1,-0.3 -4,-0.1 -2,-0.2 0.614 97.9 85.4 -86.8 -4.5 50.8 34.4 63.5 8 34 A Q S < S- 0 0 27 -3,-1.8 2,-2.2 -4,-0.4 -3,-0.0 -0.511 94.6-102.3-101.7 147.7 47.7 32.9 65.2 9 35 A P S S+ 0 0 26 0, 0.0 359,-2.2 0, 0.0 360,-0.4 -0.505 84.4 110.2 -69.9 66.8 46.2 29.2 65.0 10 36 A V S S- 0 0 4 -2,-2.2 168,-0.1 -5,-0.3 357,-0.1 -0.879 77.3 -80.5-130.6 165.6 43.4 30.2 62.5 11 37 A P - 0 0 13 0, 0.0 2,-0.1 0, 0.0 -3,-0.1 -0.379 42.3-116.5 -65.7 146.8 43.1 29.2 58.8 12 38 A K > - 0 0 84 165,-0.1 3,-0.9 1,-0.1 166,-0.2 -0.363 38.5 -97.3 -70.2 163.3 45.0 31.0 56.1 13 39 A I T 3 S+ 0 0 45 1,-0.3 -1,-0.1 164,-0.1 165,-0.0 0.750 125.0 40.5 -57.9 -24.5 42.9 33.0 53.6 14 40 A N T 3 S+ 0 0 141 2,-0.0 -1,-0.3 164,-0.0 2,-0.0 0.594 84.1 119.3 -99.7 -12.7 43.1 30.0 51.1 15 41 A D < + 0 0 54 -3,-0.9 2,-0.4 1,-0.0 163,-0.1 -0.275 34.5 175.7 -56.3 131.2 42.7 27.1 53.5 16 42 A E - 0 0 71 356,-0.1 2,-0.3 354,-0.0 356,-0.1 -0.973 12.4-156.6-138.8 126.5 39.6 24.9 52.8 17 43 A F - 0 0 43 354,-0.4 356,-0.2 -2,-0.4 685,-0.1 -0.820 17.1-113.0-109.8 143.8 38.9 21.7 54.8 18 44 A N > - 0 0 41 -2,-0.3 3,-0.9 1,-0.1 354,-0.5 -0.195 34.4-110.7 -54.0 156.0 37.0 18.5 54.1 19 45 A E T 3 S+ 0 0 105 683,-0.4 -1,-0.1 1,-0.2 353,-0.1 0.705 118.6 61.7 -63.8 -17.1 33.9 17.8 56.2 20 46 A S T 3 S+ 0 0 97 680,-0.1 2,-0.3 682,-0.1 -1,-0.2 0.788 83.8 86.6 -83.8 -26.2 35.8 15.0 57.8 21 47 A V < + 0 0 35 -3,-0.9 351,-0.7 1,-0.0 2,-0.1 -0.607 46.5 150.1 -69.8 124.8 38.5 17.4 59.3 22 48 A N E +A 371 0A 15 -2,-0.3 349,-0.2 349,-0.2 102,-0.0 -0.526 30.7 96.7-161.7 84.6 37.3 18.7 62.7 23 49 A E E S-A 370 0A 82 347,-2.2 347,-3.6 -2,-0.1 -2,-0.0 -0.982 70.1 -70.7-166.4 165.1 40.1 19.4 65.1 24 50 A P - 0 0 20 0, 0.0 345,-0.3 0, 0.0 3,-0.2 -0.336 28.2-135.8 -66.7 154.9 42.4 22.0 66.7 25 51 A F S S+ 0 0 56 1,-0.3 2,-0.4 -16,-0.3 344,-0.1 0.823 103.2 29.2 -68.9 -33.7 45.2 23.6 64.7 26 52 A I S S- 0 0 87 342,-0.2 -1,-0.3 140,-0.2 3,-0.2 -0.960 81.5-179.5-135.7 107.0 47.4 23.0 67.8 27 53 A S + 0 0 69 -2,-0.4 -4,-0.0 -3,-0.2 341,-0.0 -0.462 55.8 42.2 -97.9 177.7 46.6 20.1 70.1 28 54 A D S S+ 0 0 162 -2,-0.2 -1,-0.2 1,-0.1 2,-0.2 0.921 72.2 174.2 54.0 48.3 48.1 18.8 73.3 29 55 A N - 0 0 43 -3,-0.2 2,-0.5 135,-0.1 -1,-0.1 -0.534 21.2-140.1 -81.3 157.9 48.6 22.2 74.8 30 56 A K >> - 0 0 135 -2,-0.2 3,-1.7 1,-0.1 4,-0.5 -0.969 11.2-153.4-129.3 120.9 49.8 22.4 78.4 31 57 A V G >4 S+ 0 0 49 -2,-0.5 3,-1.5 1,-0.3 -1,-0.1 0.875 96.4 65.9 -54.8 -38.4 48.5 24.9 81.0 32 58 A E G 34 S+ 0 0 160 1,-0.3 -1,-0.3 -3,-0.1 130,-0.0 0.692 100.3 50.3 -57.6 -20.5 51.9 24.6 82.8 33 59 A D G <4 S+ 0 0 126 -3,-1.7 -1,-0.3 2,-0.1 -2,-0.2 0.519 81.0 117.1-100.2 -6.5 53.7 26.3 79.8 34 60 A V S << S- 0 0 16 -3,-1.5 2,-0.1 -4,-0.5 124,-0.0 -0.333 78.0-101.5 -65.0 137.5 51.3 29.3 79.5 35 61 A R - 0 0 132 1,-0.1 -1,-0.1 2,-0.1 127,-0.1 -0.438 26.6-160.1 -60.5 138.0 53.0 32.7 80.1 36 62 A K + 0 0 130 -2,-0.1 2,-0.3 -3,-0.1 -1,-0.1 0.599 65.8 88.1 -93.9 -13.7 52.4 34.1 83.6 37 63 A D S S- 0 0 84 1,-0.1 3,-0.1 3,-0.0 -2,-0.1 -0.621 81.9-116.8 -90.0 142.6 53.3 37.6 82.8 38 64 A E - 0 0 102 -2,-0.3 -2,-0.1 1,-0.1 3,-0.1 -0.431 41.9 -94.3 -71.5 152.7 50.7 40.1 81.3 39 65 A Y - 0 0 67 112,-0.2 2,-0.3 1,-0.1 -1,-0.1 -0.345 45.5-102.7 -66.5 152.4 51.4 41.4 77.8 40 66 A K - 0 0 181 -3,-0.1 -1,-0.1 6,-0.0 6,-0.1 -0.545 35.7-159.5 -83.0 128.2 53.3 44.7 77.7 41 67 A L - 0 0 19 -2,-0.3 3,-0.1 4,-0.2 4,-0.1 -0.692 35.1 -80.3 -98.5 161.4 51.2 47.7 76.9 42 68 A P > - 0 0 51 0, 0.0 3,-1.4 0, 0.0 -1,-0.1 -0.220 67.4 -73.8 -56.8 155.0 52.5 51.0 75.5 43 69 A P T 3 S+ 0 0 123 0, 0.0 3,-0.1 0, 0.0 -3,-0.0 -0.229 120.7 24.0 -51.8 134.9 54.1 53.5 78.0 44 70 A G T 3 S+ 0 0 34 1,-0.3 62,-2.7 -3,-0.1 63,-0.5 0.483 101.1 100.2 87.8 7.9 51.4 55.2 80.2 45 71 A Y E < -B 105 0B 43 -3,-1.4 2,-0.3 60,-0.2 -1,-0.3 -0.930 49.7-170.0-123.2 142.7 48.7 52.5 79.8 46 72 A S E -B 104 0B 52 58,-2.2 58,-3.0 -2,-0.3 -6,-0.0 -0.992 27.3-107.1-135.2 148.8 47.8 49.8 82.4 47 73 A W E -B 103 0B 37 -2,-0.3 2,-0.4 56,-0.2 56,-0.2 -0.349 35.8-167.1 -62.6 147.7 45.7 46.6 82.6 48 74 A Y E -B 102 0B 68 54,-2.0 54,-2.8 -2,-0.0 2,-0.8 -0.985 18.7-136.4-141.2 131.6 42.7 47.1 84.8 49 75 A V E -B 101 0B 59 -2,-0.4 2,-0.3 52,-0.2 52,-0.2 -0.772 25.9-146.3 -89.5 112.6 40.5 44.3 86.1 50 76 A C - 0 0 1 50,-1.7 2,-0.9 -2,-0.8 50,-0.2 -0.597 5.4-149.1 -79.1 133.2 36.8 45.4 85.6 51 77 A D > - 0 0 50 -2,-0.3 3,-3.0 1,-0.2 7,-0.6 -0.809 11.3-172.4 -96.8 91.0 34.2 44.4 88.2 52 78 A V T 3 S+ 0 0 3 -2,-0.9 -1,-0.2 1,-0.3 6,-0.1 0.797 84.4 61.4 -54.1 -27.9 31.1 44.1 85.9 53 79 A K T 3 S+ 0 0 121 -3,-0.1 2,-0.6 4,-0.0 -1,-0.3 0.566 87.1 84.5 -74.8 -5.4 29.1 43.6 89.2 54 80 A D S <> S- 0 0 68 -3,-3.0 4,-2.6 1,-0.2 5,-0.3 -0.876 77.5-141.4 -99.1 126.8 30.1 47.0 90.5 55 81 A E H > S+ 0 0 124 -2,-0.6 4,-2.3 1,-0.2 -1,-0.2 0.897 99.4 48.0 -51.8 -45.4 27.8 49.8 89.1 56 82 A K H > S+ 0 0 146 2,-0.2 4,-1.5 1,-0.2 -1,-0.2 0.944 113.3 43.8 -67.2 -45.0 30.7 52.2 88.7 57 83 A D H >> S+ 0 0 18 -6,-0.2 4,-1.8 1,-0.2 3,-0.6 0.950 114.8 52.9 -61.1 -46.7 33.2 49.9 86.8 58 84 A R H 3X S+ 0 0 40 -4,-2.6 4,-2.5 -7,-0.6 -1,-0.2 0.867 107.0 51.3 -58.6 -39.6 30.2 48.7 84.8 59 85 A S H 3X S+ 0 0 38 -4,-2.3 4,-2.2 -5,-0.3 -1,-0.3 0.805 105.4 55.4 -70.3 -25.8 29.4 52.3 83.9 60 86 A E H - 0 0 46 5,-1.3 3,-1.4 -2,-0.3 -1,-0.1 -0.274 44.2 -94.1 -75.9 173.4 27.4 50.9 60.5 73 99 A D T 3 S+ 0 0 110 1,-0.3 -1,-0.1 2,-0.1 -2,-0.0 0.745 127.5 49.3 -66.2 -27.5 29.3 51.8 57.2 74 100 A D T 3 S- 0 0 112 3,-0.0 -1,-0.3 0, 0.0 -2,-0.0 0.538 104.7-133.4 -85.9 1.1 27.5 55.1 56.8 75 101 A N S < S+ 0 0 91 -3,-1.4 -2,-0.1 2,-0.1 3,-0.1 0.911 71.4 121.4 35.7 55.5 28.2 56.0 60.5 76 102 A I S S+ 0 0 53 1,-0.2 220,-3.4 219,-0.0 2,-0.4 0.590 70.9 43.4 -96.4 -26.8 24.7 57.0 61.2 77 103 A F E - R 0 295C 22 218,-0.2 -5,-1.3 226,-0.0 2,-0.4 -0.943 59.5-170.7-123.8 142.2 24.2 54.5 63.9 78 104 A R E -QR 71 294C 29 216,-2.5 216,-3.0 -2,-0.4 -7,-0.2 -0.998 34.9-109.0-128.7 133.6 26.3 53.3 66.8 79 105 A F E - R 0 293C 52 -9,-2.6 -10,-0.3 -2,-0.4 214,-0.3 -0.369 30.4-169.4 -56.0 139.0 25.5 50.2 69.0 80 106 A N - 0 0 22 212,-3.1 -1,-0.1 -13,-0.0 -14,-0.1 -0.521 3.7-173.1-133.1 65.0 24.4 51.4 72.5 81 107 A Y - 0 0 7 210,-0.1 210,-0.1 -2,-0.1 -15,-0.1 -0.341 27.1-122.8 -60.7 133.0 24.3 48.3 74.7 82 108 A S > - 0 0 28 208,-0.3 4,-2.1 187,-0.2 5,-0.2 -0.342 15.0-113.3 -78.3 159.3 22.8 49.3 78.0 83 109 A A H > S+ 0 0 31 2,-0.2 4,-2.3 1,-0.2 5,-0.1 0.878 118.3 51.7 -54.5 -43.8 24.6 48.8 81.3 84 110 A E H > S+ 0 0 98 1,-0.2 4,-2.1 2,-0.2 -1,-0.2 0.909 108.4 53.0 -70.5 -35.2 22.1 46.2 82.5 85 111 A F H > S+ 0 0 0 1,-0.2 4,-3.0 2,-0.2 5,-0.2 0.935 107.9 51.0 -57.3 -46.0 22.5 44.3 79.2 86 112 A L H X S+ 0 0 1 -4,-2.1 4,-1.9 2,-0.2 -2,-0.2 0.921 106.5 54.5 -64.4 -35.8 26.3 44.2 79.7 87 113 A L H X S+ 0 0 52 -4,-2.3 4,-1.2 1,-0.2 3,-0.4 0.979 112.4 42.6 -60.8 -53.1 25.8 42.9 83.2 88 114 A W H < S+ 0 0 7 -4,-2.1 3,-0.3 1,-0.2 -2,-0.2 0.915 114.7 51.3 -52.3 -45.8 23.7 39.9 81.8 89 115 A A H < S+ 0 0 4 -4,-3.0 3,-0.3 1,-0.2 -1,-0.2 0.817 117.2 37.9 -62.4 -31.7 26.1 39.3 78.9 90 116 A L H < S+ 0 0 2 -4,-1.9 -1,-0.2 -3,-0.4 -2,-0.2 0.573 109.9 57.4-103.0 -9.5 29.2 39.1 81.1 91 117 A T < + 0 0 22 -4,-1.2 -1,-0.2 -3,-0.3 3,-0.1 -0.050 67.5 140.0-118.8 29.4 27.9 37.3 84.2 92 118 A S > - 0 0 2 -3,-0.3 3,-2.1 1,-0.2 167,-0.1 -0.150 69.3 -52.4 -59.0 170.6 26.6 34.0 82.8 93 119 A P T 3 S+ 0 0 29 0, 0.0 -1,-0.2 0, 0.0 166,-0.0 -0.157 126.4 4.0 -55.0 130.2 27.2 30.7 84.8 94 120 A N T 3 S+ 0 0 142 1,-0.2 -2,-0.1 -3,-0.1 26,-0.0 0.556 88.2 162.8 75.1 12.0 30.8 30.2 85.6 95 121 A Y < - 0 0 67 -3,-2.1 2,-0.5 23,-0.1 -1,-0.2 -0.166 31.8-134.7 -58.8 154.5 32.0 33.5 84.2 96 122 A L > - 0 0 34 21,-0.3 3,-1.6 1,-0.1 21,-0.2 -0.978 6.0-149.1-117.5 123.8 35.4 34.7 85.3 97 123 A K G > S+ 0 0 100 -2,-0.5 3,-2.0 1,-0.2 -47,-0.2 0.794 98.7 64.0 -47.4 -35.9 36.0 38.3 86.5 98 124 A T G 3 S+ 0 0 65 1,-0.3 -1,-0.2 -49,-0.1 61,-0.1 0.539 91.9 63.7 -82.4 -1.1 39.6 38.0 85.1 99 125 A W G < S+ 0 0 0 -3,-1.6 18,-1.4 18,-0.1 2,-0.6 0.217 83.9 82.3 -95.3 11.8 38.2 37.6 81.6 100 126 A H E < S- C 0 116B 1 -3,-2.0 -50,-1.7 16,-0.2 2,-0.5 -0.899 70.9-178.6-116.0 90.6 36.7 41.1 81.8 101 127 A I E +BC 49 115B 0 14,-2.2 14,-1.8 -2,-0.6 2,-0.2 -0.828 11.5 179.9-108.7 118.3 39.9 43.1 80.8 102 128 A G E -BC 48 114B 0 -54,-2.8 -54,-2.0 -2,-0.5 2,-0.4 -0.664 16.3-147.4-103.0 172.5 40.2 46.9 80.6 103 129 A V E -BC 47 113B 3 10,-1.9 9,-2.7 -56,-0.2 10,-1.1 -0.997 17.9-162.6-137.5 129.6 43.1 49.1 79.7 104 130 A K E -BC 46 111B 14 -58,-3.0 -58,-2.2 -2,-0.4 2,-0.7 -0.842 24.0-124.3-110.6 155.6 43.4 52.6 81.3 105 131 A Y E >> -BC 45 110B 84 5,-1.9 4,-2.5 -2,-0.3 5,-0.7 -0.889 26.0-146.6 -92.0 119.1 45.4 55.6 80.5 106 132 A D T 45S+ 0 0 80 -62,-2.7 -1,-0.2 -2,-0.7 -61,-0.1 0.898 90.6 55.7 -57.6 -45.0 47.3 56.2 83.7 107 133 A A T 45S+ 0 0 84 -63,-0.5 -1,-0.2 1,-0.2 -62,-0.1 0.857 122.7 24.7 -63.1 -40.4 47.4 60.0 83.5 108 134 A S T 45S- 0 0 57 -3,-0.3 -1,-0.2 2,-0.1 -2,-0.2 0.636 94.2-133.9 -94.2 -14.5 43.5 60.5 83.3 109 135 A N T <5 + 0 0 104 -4,-2.5 2,-0.3 1,-0.2 -3,-0.2 0.685 58.0 147.4 64.9 22.2 42.6 57.1 85.0 110 136 A K E < -C 105 0B 96 -5,-0.7 -5,-1.9 1,-0.0 2,-0.4 -0.609 56.0-117.5 -93.6 139.4 40.1 56.8 82.1 111 137 A L E +C 104 0B 10 -2,-0.3 -7,-0.2 -7,-0.2 3,-0.1 -0.682 41.4 162.8 -75.8 123.0 39.1 53.4 80.5 112 138 A I E + 0 0 4 -9,-2.7 28,-2.8 -2,-0.4 2,-0.3 0.347 59.1 31.8-125.3 1.2 40.3 53.5 76.9 113 139 A G E +CD 103 139B 1 -10,-1.1 -10,-1.9 26,-0.2 2,-0.3 -0.980 57.6 173.7-157.8 151.5 40.2 49.7 75.9 114 140 A F E +CD 102 138B 0 24,-1.8 24,-2.4 -2,-0.3 2,-0.3 -0.981 6.8 174.3-155.4 153.1 38.2 46.6 76.8 115 141 A I E -C 101 0B 2 -14,-1.8 -14,-2.2 -2,-0.3 2,-0.3 -0.947 13.8-170.0-155.7 144.0 37.9 42.9 75.8 116 142 A S E -CD 100 135B 1 19,-1.9 19,-1.8 -2,-0.3 2,-0.3 -0.924 10.7-173.9-134.1 158.2 35.7 40.2 77.3 117 143 A A E - 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