==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=28-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM/PEPTIDE 23-MAY-05 2BSS . COMPND 2 MOLECULE: HLA CLASS I HISTOCOMPATIBILITY ANTIGEN B-27 . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR G.B.E.STEWART-JONES,K.DI GLERIA,S.KOLLNBERGER,A.J.MCMICHAEL, . 386 3 3 3 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19395.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 256 66.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 135 35.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 25 6.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 31 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 59 15.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 7 2 2 0 4 0 2 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 2 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G 0 0 71 0, 0.0 2,-0.2 0, 0.0 102,-0.0 0.000 360.0 360.0 360.0-130.9 2.3 26.1 29.6 2 2 A S - 0 0 70 102,-0.1 2,-0.3 104,-0.1 102,-0.2 -0.547 360.0-167.9 -90.5 156.3 5.9 25.2 28.9 3 3 A H E -A 103 0A 32 100,-2.4 100,-2.4 -2,-0.2 2,-0.3 -0.883 4.3-156.7-137.8 168.7 7.1 21.9 27.5 4 4 A S E -A 102 0A 24 -2,-0.3 25,-2.7 98,-0.2 2,-0.4 -0.977 7.7-156.0-147.6 158.3 10.3 19.9 27.0 5 5 A M E +AB 101 28A 0 96,-2.3 96,-0.9 -2,-0.3 2,-0.4 -0.998 20.1 178.4-134.8 135.2 11.9 17.2 25.0 6 6 A R E -AB 100 27A 33 21,-2.7 21,-3.1 -2,-0.4 2,-0.5 -1.000 20.9-158.3-143.7 142.8 14.8 15.1 26.1 7 7 A Y E -AB 99 26A 0 92,-2.4 92,-1.6 -2,-0.4 2,-0.4 -0.982 16.8-164.0-117.9 126.1 16.9 12.2 24.9 8 8 A F E -AB 98 25A 30 17,-2.9 17,-1.8 -2,-0.5 2,-0.4 -0.928 6.3-174.0-113.2 134.6 18.8 10.2 27.5 9 9 A H E -AB 97 24A 4 88,-2.9 88,-2.5 -2,-0.4 2,-0.4 -0.992 6.3-179.2-133.6 136.9 21.7 7.8 26.7 10 10 A T E -AB 96 23A 4 13,-2.4 13,-2.5 -2,-0.4 2,-0.4 -0.999 3.6-176.0-135.6 131.9 23.7 5.4 28.9 11 11 A S E -AB 95 22A 2 84,-2.3 84,-2.5 -2,-0.4 2,-0.5 -0.999 6.8-172.2-126.5 128.8 26.5 3.2 27.9 12 12 A V E -AB 94 21A 13 9,-2.2 9,-2.4 -2,-0.4 82,-0.2 -0.973 14.8-141.7-126.5 117.3 28.0 0.8 30.4 13 13 A S - 0 0 16 80,-2.4 6,-0.1 -2,-0.5 5,-0.0 -0.414 14.2-174.1 -74.0 153.2 31.2 -1.2 29.8 14 14 A R > - 0 0 42 4,-0.5 3,-2.5 78,-0.1 78,-0.1 -0.543 16.7-149.8-148.7 71.4 31.3 -4.8 31.1 15 15 A P T 3 S+ 0 0 58 0, 0.0 4,-0.1 0, 0.0 77,-0.1 -0.126 83.5 18.0 -47.0 132.5 34.9 -6.2 30.4 16 16 A G T 3 S+ 0 0 88 2,-0.1 3,-0.1 1,-0.0 74,-0.0 0.310 115.3 76.4 86.4 -9.4 34.8 -10.0 29.9 17 17 A R S < S- 0 0 157 -3,-2.5 2,-0.1 1,-0.5 -1,-0.0 -0.137 105.4 -91.2-126.4 38.0 31.1 -9.9 29.2 18 18 A G S S- 0 0 56 -5,-0.0 -1,-0.5 3,-0.0 -4,-0.5 -0.340 70.8 -22.0 88.7-171.1 30.9 -8.6 25.6 19 19 A E S S- 0 0 88 1,-0.2 -7,-0.1 -6,-0.1 56,-0.0 -0.253 84.0 -74.6 -72.4 161.9 30.6 -5.0 24.3 20 20 A P - 0 0 13 0, 0.0 2,-0.3 0, 0.0 -7,-0.3 -0.228 48.2-117.4 -58.1 141.9 29.2 -2.1 26.3 21 21 A R E -B 12 0A 83 -9,-2.4 -9,-2.2 -3,-0.1 2,-0.4 -0.618 29.0-159.7 -81.3 142.6 25.4 -2.1 26.9 22 22 A F E +B 11 0A 4 -2,-0.3 16,-3.2 -11,-0.2 2,-0.4 -0.985 14.7 171.4-130.7 126.0 23.6 0.9 25.4 23 23 A I E +BC 10 37A 14 -13,-2.5 -13,-2.4 -2,-0.4 2,-0.4 -0.997 2.3 172.8-134.6 131.0 20.2 2.2 26.4 24 24 A T E -BC 9 36A 0 12,-2.4 12,-1.9 -2,-0.4 2,-0.3 -0.995 4.6-176.9-140.2 144.4 18.5 5.4 25.3 25 25 A V E -BC 8 35A 0 -17,-1.8 -17,-2.9 -2,-0.4 2,-0.4 -0.955 14.3-143.1-137.5 153.6 15.0 6.8 25.8 26 26 A G E -BC 7 34A 0 8,-2.5 7,-2.8 -2,-0.3 8,-1.2 -0.959 16.6-172.0-121.5 141.8 13.2 9.9 24.6 27 27 A Y E -BC 6 32A 33 -21,-3.1 -21,-2.7 -2,-0.4 2,-0.5 -0.962 21.4-157.8-132.0 149.1 10.7 11.9 26.6 28 28 A V E > S-BC 5 31A 0 3,-2.0 3,-2.6 -2,-0.3 -23,-0.3 -0.999 82.7 -43.7-120.0 118.0 8.4 14.8 25.9 29 29 A D T 3 S- 0 0 28 -25,-2.7 -23,-0.1 -2,-0.5 -26,-0.1 -0.386 125.2 -26.8 54.8-122.6 7.8 16.4 29.3 30 30 A D T 3 S+ 0 0 63 -2,-0.1 2,-0.6 -3,-0.1 -1,-0.3 0.143 115.3 103.9-107.8 21.3 7.2 13.3 31.6 31 31 A T E < -C 28 0A 22 -3,-2.6 -3,-2.0 148,-0.0 2,-0.3 -0.904 65.4-139.6-107.5 119.9 5.9 11.0 28.9 32 32 A L E +C 27 0A 48 -2,-0.6 -5,-0.2 -5,-0.2 3,-0.1 -0.587 30.7 164.5 -73.6 130.3 8.3 8.3 27.5 33 33 A F E + 0 0 0 -7,-2.8 15,-2.6 1,-0.4 16,-0.8 0.651 55.6 24.2-124.1 -20.0 7.8 8.0 23.8 34 34 A V E -CD 26 47A 0 -8,-1.2 -8,-2.5 13,-0.3 -1,-0.4 -0.995 57.2-178.2-146.1 150.5 10.8 6.1 22.3 35 35 A R E -CD 25 46A 30 11,-1.8 11,-2.8 -2,-0.3 2,-0.3 -0.975 7.1-174.6-145.2 156.6 13.3 3.5 23.5 36 36 A F E -C 24 0A 3 -12,-1.9 -12,-2.4 -2,-0.3 2,-0.4 -0.953 2.2-176.3-153.0 132.1 16.3 1.6 22.2 37 37 A D E > -C 23 0A 9 -2,-0.3 3,-2.3 7,-0.3 -14,-0.2 -0.962 21.2-149.8-135.6 115.9 18.3 -1.1 24.0 38 38 A S T 3 S+ 0 0 27 -16,-3.2 -15,-0.1 -2,-0.4 -1,-0.1 0.666 98.4 59.9 -58.6 -15.4 21.4 -2.7 22.3 39 39 A D T 3 S+ 0 0 75 -17,-0.3 -1,-0.3 3,-0.1 -16,-0.1 0.493 80.0 110.4 -92.1 -4.3 20.6 -5.9 24.2 40 40 A A S < S- 0 0 29 -3,-2.3 -4,-0.0 1,-0.1 0, 0.0 -0.278 85.2-102.5 -67.5 158.3 17.2 -6.4 22.6 41 41 A A S S+ 0 0 106 1,-0.3 -1,-0.1 3,-0.0 -3,-0.0 0.932 113.5 7.1 -44.9 -65.5 16.7 -9.3 20.2 42 42 A S S S- 0 0 105 2,-0.1 -1,-0.3 0, 0.0 -3,-0.1 -0.696 95.0-132.3-124.0 76.9 16.9 -7.2 17.0 43 43 A P + 0 0 35 0, 0.0 2,-0.3 0, 0.0 -5,-0.1 0.064 46.3 143.8 -33.8 116.0 17.8 -3.7 18.3 44 44 A R - 0 0 100 -7,-0.2 2,-0.4 -9,-0.1 -7,-0.3 -0.976 56.6 -97.9-154.5 149.1 15.5 -1.0 16.8 45 45 A E - 0 0 5 15,-0.4 -9,-0.2 -2,-0.3 19,-0.1 -0.626 46.7-161.1 -74.8 129.9 14.0 2.2 18.2 46 46 A E E -D 35 0A 66 -11,-2.8 -11,-1.8 -2,-0.4 2,-0.1 -0.845 17.5-113.0-115.2 150.0 10.5 1.4 19.4 47 47 A P E +D 34 0A 41 0, 0.0 -13,-0.3 0, 0.0 -14,-0.1 -0.434 26.0 178.1 -75.1 150.2 7.5 3.6 20.1 48 48 A R + 0 0 108 -15,-2.6 -14,-0.2 1,-0.2 -16,-0.1 0.246 67.1 54.8-134.1 6.0 6.3 3.9 23.7 49 49 A A S >> S- 0 0 4 -16,-0.8 3,-1.1 1,-0.1 4,-0.7 -0.989 78.9-128.3-139.3 145.3 3.5 6.4 23.2 50 50 A P H >> S+ 0 0 93 0, 0.0 3,-1.0 0, 0.0 4,-0.5 0.880 102.9 61.5 -62.3 -40.2 0.6 6.2 20.8 51 51 A W H 34 S+ 0 0 12 1,-0.2 125,-0.1 2,-0.2 123,-0.1 0.579 102.5 52.2 -66.9 -8.4 1.0 9.6 19.2 52 52 A I H X4 S+ 0 0 0 -3,-1.1 3,-2.1 1,-0.1 -1,-0.2 0.666 87.7 79.8 -98.8 -19.7 4.5 8.6 17.8 53 53 A E H << S+ 0 0 123 -3,-1.0 -2,-0.2 -4,-0.7 -1,-0.1 0.804 80.4 68.9 -57.0 -31.7 3.4 5.4 16.1 54 54 A Q T 3< S+ 0 0 125 -4,-0.5 -1,-0.3 2,-0.0 -2,-0.1 0.645 74.6 110.3 -64.5 -14.4 2.0 7.4 13.2 55 55 A E S < S- 0 0 14 -3,-2.1 -3,-0.0 1,-0.1 5,-0.0 -0.333 73.6-111.2 -61.8 146.0 5.6 8.3 12.2 56 56 A G >> - 0 0 21 1,-0.1 4,-1.8 3,-0.0 3,-1.1 -0.284 23.1 -99.2 -82.2 164.9 6.7 6.6 9.0 57 57 A P H 3> S+ 0 0 84 0, 0.0 4,-2.4 0, 0.0 5,-0.2 0.798 123.2 58.3 -47.4 -35.0 9.3 3.9 8.3 58 58 A E H 3> S+ 0 0 134 2,-0.2 4,-2.2 1,-0.2 5,-0.2 0.913 104.4 51.1 -64.3 -42.1 11.8 6.5 7.2 59 59 A Y H <> S+ 0 0 18 -3,-1.1 4,-2.4 1,-0.2 -1,-0.2 0.964 113.4 44.2 -58.4 -52.8 11.5 8.1 10.7 60 60 A W H X S+ 0 0 28 -4,-1.8 4,-2.4 1,-0.2 -15,-0.4 0.823 111.4 52.4 -64.9 -33.5 12.1 4.8 12.4 61 61 A D H X S+ 0 0 79 -4,-2.4 4,-2.3 -5,-0.2 -1,-0.2 0.920 112.1 45.5 -68.8 -43.4 15.0 3.8 10.2 62 62 A R H X S+ 0 0 95 -4,-2.2 4,-2.8 2,-0.2 -2,-0.2 0.929 114.1 49.2 -64.7 -44.9 16.8 7.1 10.7 63 63 A E H X S+ 0 0 1 -4,-2.4 4,-2.6 -5,-0.2 -2,-0.2 0.929 112.3 47.6 -60.0 -46.6 16.2 7.0 14.5 64 64 A T H X S+ 0 0 5 -4,-2.4 4,-2.3 1,-0.2 -1,-0.2 0.904 111.8 51.3 -61.6 -41.7 17.5 3.4 14.7 65 65 A Q H X S+ 0 0 133 -4,-2.3 4,-2.3 2,-0.2 -2,-0.2 0.929 110.4 48.3 -61.1 -46.6 20.5 4.4 12.6 66 66 A I H X S+ 0 0 9 -4,-2.8 4,-2.8 1,-0.2 5,-0.2 0.919 109.8 52.5 -61.2 -43.8 21.3 7.3 14.9 67 67 A C H X S+ 0 0 0 -4,-2.6 4,-2.4 1,-0.2 -1,-0.2 0.921 110.9 46.8 -59.4 -45.2 20.9 5.1 18.0 68 68 A K H X S+ 0 0 111 -4,-2.3 4,-2.2 2,-0.2 -1,-0.2 0.885 111.5 51.2 -65.2 -39.0 23.4 2.6 16.6 69 69 A A H X S+ 0 0 41 -4,-2.3 4,-2.7 2,-0.2 5,-0.2 0.941 111.0 48.2 -63.4 -46.1 25.8 5.3 15.6 70 70 A K H X S+ 0 0 30 -4,-2.8 4,-2.2 1,-0.2 -2,-0.2 0.912 109.0 54.4 -59.4 -43.0 25.7 6.8 19.1 71 71 A A H X S+ 0 0 10 -4,-2.4 4,-1.6 -5,-0.2 -1,-0.2 0.914 110.6 46.7 -57.7 -42.6 26.2 3.3 20.6 72 72 A Q H X S+ 0 0 81 -4,-2.2 4,-2.0 1,-0.2 -2,-0.2 0.916 110.4 50.0 -67.0 -45.0 29.3 2.9 18.5 73 73 A T H X S+ 0 0 23 -4,-2.7 4,-2.7 1,-0.2 315,-0.3 0.841 108.2 55.3 -64.7 -30.7 30.8 6.3 19.3 74 74 A D H X S+ 0 0 2 -4,-2.2 4,-2.7 -5,-0.2 -1,-0.2 0.906 104.9 52.6 -67.9 -40.1 30.2 5.6 23.0 75 75 A R H X S+ 0 0 63 -4,-1.6 4,-1.5 2,-0.2 -2,-0.2 0.939 112.8 44.7 -59.7 -46.6 32.2 2.3 22.7 76 76 A E H X S+ 0 0 70 -4,-2.0 4,-2.3 1,-0.2 -2,-0.2 0.929 113.7 50.2 -62.8 -44.9 35.1 4.3 21.2 77 77 A D H X S+ 0 0 3 -4,-2.7 4,-3.0 1,-0.2 -2,-0.2 0.851 104.8 56.7 -64.3 -35.9 34.8 7.1 23.8 78 78 A L H X S+ 0 0 9 -4,-2.7 4,-1.1 2,-0.2 -1,-0.2 0.904 110.2 45.6 -61.4 -40.4 34.8 4.6 26.7 79 79 A R H X S+ 0 0 124 -4,-1.5 4,-1.0 -5,-0.2 3,-0.3 0.917 113.1 51.1 -67.3 -43.0 38.2 3.3 25.4 80 80 A T H >X S+ 0 0 17 -4,-2.3 4,-2.1 1,-0.2 3,-0.7 0.917 106.7 52.1 -60.7 -46.0 39.4 6.9 24.9 81 81 A L H 3X S+ 0 0 0 -4,-3.0 4,-2.2 1,-0.3 -1,-0.2 0.778 98.2 65.4 -65.8 -24.3 38.5 8.0 28.5 82 82 A L H 3<>S+ 0 0 27 -4,-1.1 5,-3.2 -3,-0.3 -1,-0.3 0.904 107.5 43.5 -61.6 -37.9 40.4 5.0 29.9 83 83 A R H X<5S+ 0 0 153 -4,-1.0 3,-2.0 -3,-0.7 -2,-0.2 0.955 109.7 54.3 -69.5 -52.4 43.5 6.8 28.5 84 84 A Y H 3<5S+ 0 0 44 -4,-2.1 -2,-0.2 1,-0.3 -1,-0.2 0.823 118.5 36.3 -51.1 -36.7 42.5 10.3 29.6 85 85 A Y T 3<5S- 0 0 61 -4,-2.2 -1,-0.3 -5,-0.2 -2,-0.2 0.283 106.8-122.5-103.6 11.2 42.1 9.0 33.2 86 86 A N T < 5 + 0 0 155 -3,-2.0 2,-0.3 1,-0.2 -3,-0.2 0.940 59.1 156.9 47.5 53.9 45.0 6.5 33.1 87 87 A Q < - 0 0 62 -5,-3.2 -1,-0.2 -6,-0.1 2,-0.1 -0.780 43.2-108.9-110.6 156.3 42.6 3.7 34.0 88 88 A S > - 0 0 78 -2,-0.3 3,-0.6 1,-0.1 -1,-0.0 -0.423 19.8-125.9 -80.1 157.3 42.8 -0.0 33.5 89 89 A E T 3 S+ 0 0 160 1,-0.2 -1,-0.1 -2,-0.1 -7,-0.0 0.320 96.1 82.7 -83.9 9.2 40.6 -1.9 31.0 90 90 A A T 3 S+ 0 0 81 2,-0.1 -1,-0.2 -76,-0.0 2,-0.1 0.773 77.2 73.0 -85.7 -27.9 39.5 -4.2 33.8 91 91 A G S < S- 0 0 30 -3,-0.6 2,-0.4 -77,-0.1 -77,-0.0 -0.424 80.7-110.5 -91.5 164.7 36.7 -2.2 35.4 92 92 A S - 0 0 42 -2,-0.1 2,-0.3 -78,-0.1 -78,-0.1 -0.758 37.8-173.9 -90.2 134.6 33.2 -1.2 34.3 93 93 A H - 0 0 16 -2,-0.4 -80,-2.4 2,-0.0 2,-0.4 -0.904 12.9-138.0-130.3 158.3 32.7 2.4 33.4 94 94 A T E -A 12 0A 1 -2,-0.3 25,-3.0 -82,-0.2 2,-0.5 -0.932 9.7-170.5-123.1 139.8 29.7 4.6 32.5 95 95 A L E -AE 11 118A 1 -84,-2.5 -84,-2.3 -2,-0.4 2,-0.4 -0.993 14.3-173.4-125.4 124.8 29.1 7.3 29.9 96 96 A Q E -AE 10 117A 1 21,-2.7 21,-2.2 -2,-0.5 2,-0.4 -0.946 4.4-179.5-122.2 142.9 25.9 9.3 30.1 97 97 A N E +AE 9 116A 2 -88,-2.5 -88,-2.9 -2,-0.4 2,-0.4 -0.996 5.7 175.8-144.5 138.2 24.4 11.9 27.8 98 98 A M E +AE 8 115A 20 17,-2.2 17,-2.6 -2,-0.4 2,-0.3 -0.996 9.1 161.0-137.9 139.6 21.3 14.0 27.7 99 99 A Y E +AE 7 114A 8 -92,-1.6 -92,-2.4 -2,-0.4 2,-0.3 -0.980 15.3 108.3-152.4 160.2 20.3 16.7 25.3 100 100 A G E -AE 6 113A 0 13,-1.9 13,-2.7 -2,-0.3 2,-0.3 -0.949 46.3 -90.9 158.0-174.9 17.1 18.5 24.3 101 101 A a E -AE 5 112A 0 -96,-0.9 -96,-2.3 -2,-0.3 2,-0.4 -0.896 11.7-144.6-134.7 159.9 15.1 21.7 24.4 102 102 A D E -AE 4 111A 30 9,-2.3 8,-3.5 -2,-0.3 9,-1.3 -0.959 19.7-166.8-120.0 138.4 12.4 23.6 26.4 103 103 A V E -AE 3 109A 1 -100,-2.4 -100,-2.4 -2,-0.4 6,-0.2 -0.962 15.0-127.0-128.3 146.8 9.8 25.7 24.7 104 104 A G > - 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