==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=19-JUL-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 01-JUN-05 2BTJ . COMPND 2 MOLECULE: GREEN TO RED PHOTOCONVERTIBLE GPF-LIKE PROTEIN EO . SOURCE 2 ORGANISM_SCIENTIFIC: LOBOPHYLLIA HEMPRICHII; . AUTHOR H.NAR,K.NIENHAUS,J.WIEDENMANN,G.U.NIENHAUS . 876 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33024.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 643 73.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 46.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 73 8.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 78 8.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 16 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 8 0 4 4 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G 0 0 104 0, 0.0 2,-1.1 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 -84.1 -6.2 11.2 2.4 2 2 A G + 0 0 60 2,-0.5 0, 0.0 1,-0.2 0, 0.0 -0.612 360.0 47.2 98.6 -75.3 -6.2 10.6 6.1 3 3 A A S S+ 0 0 31 -2,-1.1 2,-0.4 78,-0.1 -1,-0.2 0.901 113.7 51.5 -68.1 -42.2 -9.9 9.8 6.8 4 4 A I S S- 0 0 6 77,-0.1 -2,-0.5 74,-0.1 -1,-0.1 -0.842 80.2-142.9-102.8 133.2 -10.0 7.3 3.9 5 5 A K - 0 0 78 -2,-0.4 29,-0.2 -4,-0.1 -2,-0.0 -0.596 23.9-116.3 -91.6 153.7 -7.4 4.5 3.5 6 6 A P S S+ 0 0 92 0, 0.0 2,-0.5 0, 0.0 29,-0.2 0.750 110.8 43.7 -57.5 -24.9 -5.9 3.3 0.2 7 7 A D S S+ 0 0 97 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.975 78.0 175.7-127.0 114.7 -7.5 -0.1 1.0 8 8 A M E -A 31 0A 0 23,-3.0 23,-2.7 -2,-0.5 2,-0.2 -0.920 21.6-137.7-123.4 148.2 -11.1 -0.0 2.4 9 9 A K E -Ab 30 111A 106 101,-3.6 103,-2.8 -2,-0.3 2,-0.4 -0.641 17.3-142.9 -97.8 159.2 -13.6 -2.8 3.3 10 10 A I E -Ab 29 112A 2 19,-2.9 19,-2.3 101,-0.2 2,-0.4 -0.978 14.5-175.4-132.5 125.0 -17.3 -2.6 2.4 11 11 A N E +Ab 28 113A 75 101,-3.5 103,-2.8 -2,-0.4 2,-0.3 -0.950 19.8 170.5-114.7 132.8 -20.3 -3.8 4.4 12 12 A L E -Ab 27 114A 2 15,-2.2 15,-3.0 -2,-0.4 2,-0.4 -0.997 24.4-176.2-150.7 148.3 -23.8 -3.6 2.8 13 13 A R E -Ab 26 115A 89 101,-2.3 103,-2.8 -2,-0.3 2,-0.4 -0.979 12.2-169.2-141.4 126.2 -27.4 -4.5 3.1 14 14 A M E -Ab 25 116A 0 11,-3.2 11,-2.9 -2,-0.4 2,-0.4 -0.938 5.1-180.0-118.5 139.2 -30.1 -3.8 0.5 15 15 A E E +Ab 24 117A 55 101,-2.0 103,-1.7 -2,-0.4 2,-0.3 -0.993 22.6 127.8-134.8 140.1 -33.8 -4.2 1.0 16 16 A G E -Ab 23 118A 2 7,-1.9 7,-1.6 -2,-0.4 2,-0.3 -0.958 44.0-118.9-169.4-170.8 -36.4 -3.4 -1.7 17 17 A N E -Ab 22 119A 26 101,-1.7 103,-2.6 -2,-0.3 2,-0.5 -0.923 16.7-171.8-154.6 122.6 -39.4 -4.3 -3.8 18 18 A V E > S-Ab 21 120A 0 3,-2.1 3,-2.3 -2,-0.3 103,-0.2 -0.975 77.3 -23.0-122.9 120.2 -39.6 -4.5 -7.6 19 19 A N T 3 S- 0 0 23 101,-2.3 102,-0.1 -2,-0.5 -1,-0.1 0.739 131.1 -46.4 52.7 27.8 -43.0 -5.0 -9.3 20 20 A G T 3 S+ 0 0 37 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.329 110.5 122.8 101.1 -7.0 -44.3 -6.5 -6.1 21 21 A H E < -A 18 0A 56 -3,-2.3 -3,-2.1 2,-0.0 -1,-0.2 -0.811 48.6-151.6 -96.5 116.9 -41.4 -8.8 -5.4 22 22 A H E +A 17 0A 74 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.483 22.3 165.2 -82.8 152.3 -39.6 -8.3 -2.0 23 23 A F E -A 16 0A 0 -7,-1.6 -7,-1.9 -2,-0.2 2,-0.4 -0.989 27.0-152.2-164.7 157.4 -36.0 -9.1 -1.4 24 24 A V E -AC 15 46A 3 22,-1.8 21,-2.7 -2,-0.3 22,-1.7 -1.000 16.0-173.0-135.7 132.2 -33.0 -8.6 0.9 25 25 A I E -AC 14 44A 0 -11,-2.9 -11,-3.2 -2,-0.4 2,-0.3 -0.987 7.2-157.5-134.9 127.6 -29.4 -8.7 -0.3 26 26 A D E -AC 13 43A 59 17,-2.3 17,-2.2 -2,-0.4 2,-0.4 -0.742 11.2-162.3-100.6 149.7 -26.2 -8.6 1.8 27 27 A G E +AC 12 42A 8 -15,-3.0 -15,-2.2 -2,-0.3 2,-0.4 -0.992 15.6 179.3-138.7 143.0 -22.8 -7.5 0.5 28 28 A D E +AC 11 41A 108 13,-1.9 13,-2.9 -2,-0.4 2,-0.2 -0.979 29.4 130.3-139.3 120.2 -19.2 -7.9 1.6 29 29 A G E -AC 10 40A 5 -19,-2.3 -19,-2.9 -2,-0.4 2,-0.3 -0.839 38.8-127.7-153.0-169.2 -16.4 -6.4 -0.5 30 30 A T E -AC 9 39A 56 9,-1.9 9,-2.4 -21,-0.3 2,-0.3 -0.957 20.8-177.0-146.9 161.4 -13.2 -4.3 -0.7 31 31 A G E -AC 8 38A 3 -23,-2.7 -23,-3.0 -2,-0.3 7,-0.3 -0.996 30.9-131.9-161.2 162.6 -12.0 -1.3 -2.7 32 32 A K E >>> - C 0 37A 97 5,-2.6 5,-1.5 -2,-0.3 3,-1.3 -0.864 23.5-171.1-118.0 90.0 -9.2 1.1 -3.5 33 33 A P G >45S+ 0 0 0 0, 0.0 34,-0.8 0, 0.0 3,-0.6 0.850 84.1 51.2 -52.2 -41.4 -10.8 4.5 -3.4 34 34 A F G 345S+ 0 0 85 1,-0.2 -27,-0.1 -28,-0.2 32,-0.1 0.709 114.1 45.5 -72.8 -16.3 -7.8 6.4 -4.8 35 35 A E G <45S- 0 0 86 -3,-1.3 33,-0.3 -29,-0.2 -1,-0.2 0.497 108.9-126.8 -99.6 -8.3 -7.6 3.9 -7.7 36 36 A G T <<5 + 0 0 0 -4,-0.9 176,-2.5 -3,-0.6 2,-0.3 0.839 66.1 122.2 67.2 35.5 -11.4 4.1 -8.3 37 37 A K E < +CD 32 211A 76 -5,-1.5 -5,-2.6 174,-0.2 2,-0.3 -0.963 29.1 163.5-131.9 148.0 -12.0 0.4 -8.1 38 38 A Q E -CD 31 210A 18 172,-1.8 172,-2.2 -2,-0.3 2,-0.3 -0.988 12.8-167.1-157.5 154.2 -14.1 -1.9 -5.9 39 39 A S E -CD 30 209A 38 -9,-2.4 -9,-1.9 -2,-0.3 2,-0.3 -0.976 9.8-174.8-142.4 152.4 -15.5 -5.5 -5.9 40 40 A M E -CD 29 208A 6 168,-2.0 168,-3.2 -2,-0.3 2,-0.6 -0.997 23.3-135.1-153.2 145.4 -18.1 -7.1 -3.8 41 41 A D E -CD 28 207A 95 -13,-2.9 -13,-1.9 -2,-0.3 2,-0.3 -0.905 29.0-163.5-101.8 122.8 -19.7 -10.5 -3.1 42 42 A L E -CD 27 206A 6 164,-3.8 164,-2.0 -2,-0.6 2,-0.4 -0.832 9.8-172.4-109.1 146.8 -23.5 -10.3 -3.0 43 43 A E E -CD 26 205A 86 -17,-2.2 -17,-2.3 -2,-0.3 2,-0.6 -0.971 24.5-130.3-140.7 122.2 -25.9 -12.9 -1.6 44 44 A V E +C 25 0A 15 160,-2.6 -19,-0.3 -2,-0.4 3,-0.1 -0.602 25.9 179.8 -71.3 116.3 -29.7 -12.8 -1.9 45 45 A K E + 0 0 95 -21,-2.7 2,-0.3 -2,-0.6 -20,-0.2 0.777 66.1 13.4 -89.8 -30.7 -30.9 -13.4 1.7 46 46 A E E S+C 24 0A 100 -22,-1.7 -22,-1.8 1,-0.0 -1,-0.3 -0.988 116.1 33.2-144.1 150.8 -34.6 -13.2 1.0 47 47 A G S S- 0 0 26 -2,-0.3 -24,-0.1 1,-0.3 -1,-0.0 0.395 84.0-173.8 87.4 -4.5 -36.8 -13.2 -2.1 48 48 A G + 0 0 41 -24,-0.1 -1,-0.3 1,-0.1 2,-0.1 -0.448 58.0 46.0 -73.7 145.4 -34.5 -15.5 -4.1 49 49 A P S S- 0 0 73 0, 0.0 154,-0.1 0, 0.0 -1,-0.1 0.517 88.8-138.0 -82.9 143.4 -34.6 -16.4 -6.8 50 50 A L - 0 0 6 152,-0.5 4,-0.1 1,-0.1 -2,-0.1 -0.429 3.2-149.8 -66.8 133.7 -35.2 -12.9 -8.2 51 51 A P S S+ 0 0 49 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.276 76.5 60.6 -85.8 8.6 -37.9 -12.8 -11.0 52 52 A F S S- 0 0 2 79,-0.1 78,-0.2 80,-0.1 2,-0.2 -0.896 101.6 -72.1-134.1 164.8 -36.2 -9.8 -12.6 53 53 A A > - 0 0 3 76,-2.2 3,-1.5 -2,-0.3 4,-0.3 -0.353 32.0-146.6 -60.1 122.3 -32.8 -8.9 -14.1 54 54 A F G >> S+ 0 0 7 1,-0.3 3,-1.5 -2,-0.2 4,-1.0 0.785 90.3 78.4 -59.6 -27.2 -30.1 -8.6 -11.4 55 55 A D G 34 S+ 0 0 9 1,-0.3 4,-0.4 2,-0.2 -1,-0.3 0.760 79.7 65.5 -56.0 -29.3 -28.5 -5.9 -13.6 56 56 A I G <4 S+ 0 0 0 -3,-1.5 -1,-0.3 1,-0.2 3,-0.2 0.808 105.2 45.3 -66.7 -24.4 -31.0 -3.2 -12.5 57 57 A L T X4 S+ 0 0 0 -3,-1.5 3,-2.7 -4,-0.3 4,-0.3 0.763 86.5 89.4 -87.0 -30.1 -29.7 -3.4 -8.9 58 58 A T G >< S+ 0 0 4 -4,-1.0 3,-1.2 1,-0.3 -1,-0.1 0.742 83.7 52.6 -43.0 -42.6 -25.9 -3.4 -9.5 59 59 A T G 3 S+ 0 0 27 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.626 101.9 64.4 -73.9 -8.8 -25.3 0.4 -9.5 60 60 A A G < 0 0 1 -3,-2.7 -1,-0.2 1,-0.1 -2,-0.2 0.499 360.0 360.0 -91.0 -3.0 -27.1 0.6 -6.1 61 61 A X < 0 0 10 -3,-1.2 -1,-0.1 -4,-0.3 -3,-0.1 -0.406 360.0 360.0 -80.8 360.0 -24.6 -1.5 -4.2 62 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 63 65 A N > 0 0 7 0, 0.0 3,-2.4 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 103.6 -20.3 4.8 -3.7 64 66 A R T 3 + 0 0 12 1,-0.3 13,-0.2 13,-0.0 14,-0.2 0.428 360.0 87.6 -64.1 2.6 -18.6 7.5 -5.7 65 67 A V T 3 S+ 0 0 0 1,-0.2 2,-1.8 12,-0.2 -1,-0.3 0.858 77.7 70.3 -65.9 -34.0 -15.9 7.4 -3.1 66 68 A F S < S+ 0 0 1 -3,-2.4 2,-0.3 -30,-0.1 -1,-0.2 -0.557 78.2 101.3 -86.3 75.9 -14.5 4.7 -5.3 67 69 A A S S- 0 0 0 -2,-1.8 2,-0.8 -34,-0.8 -31,-0.3 -0.944 76.5-115.6-156.8 133.2 -13.5 6.9 -8.2 68 70 A E B -e 213 0A 86 144,-2.3 146,-2.6 -33,-0.3 -34,-0.1 -0.649 35.5-165.4 -75.9 110.2 -10.1 8.2 -9.1 69 71 A Y - 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0 0 0 -4,-2.8 94,-2.3 22,-0.1 95,-0.6 -0.561 4.7-118.2 104.1-168.7 -20.2 10.1 5.5 85 87 A Y E -FG 105 177A 0 20,-1.4 20,-2.7 92,-0.3 2,-0.3 -0.971 9.9-132.7-165.4 170.7 -23.0 11.0 3.0 86 88 A S E -FG 104 176A 20 90,-2.1 90,-2.7 -2,-0.3 2,-0.3 -0.924 13.5-160.2-133.4 157.7 -26.1 9.7 1.4 87 89 A W E -FG 103 175A 1 16,-1.9 16,-2.1 -2,-0.3 2,-0.3 -0.965 3.4-159.7-137.5 155.3 -27.4 9.6 -2.2 88 90 A E E -FG 102 174A 6 86,-1.9 86,-2.6 -2,-0.3 2,-0.3 -0.961 14.4-171.6-130.8 149.1 -30.8 9.1 -3.9 89 91 A R E -FG 101 173A 4 12,-2.7 12,-2.5 -2,-0.3 2,-0.4 -0.999 18.3-154.6-147.1 146.2 -31.3 8.0 -7.5 90 92 A S E -FG 100 172A 6 82,-1.9 82,-1.4 -2,-0.3 2,-0.6 -0.976 10.9-161.1-118.3 132.2 -34.1 7.7 -10.0 91 93 A L E -FG 99 171A 1 8,-3.1 8,-2.3 -2,-0.4 2,-0.8 -0.937 7.2-169.9-116.1 104.3 -33.7 5.2 -12.8 92 94 A T E -FG 98 170A 50 78,-2.5 78,-2.7 -2,-0.6 2,-0.2 -0.842 7.5-159.1-100.6 104.1 -36.1 6.0 -15.6 93 95 A F E > - G 0 169A 2 4,-2.4 3,-1.8 -2,-0.8 76,-0.2 -0.563 25.3-118.5 -82.2 146.2 -36.1 3.1 -18.1 94 96 A E T 3 S+ 0 0 80 74,-2.6 75,-0.1 1,-0.3 -1,-0.1 0.666 109.3 56.7 -59.2 -20.7 -37.3 3.8 -21.7 95 97 A D T 3 S- 0 0 35 73,-0.3 -1,-0.3 2,-0.1 3,-0.1 0.202 125.8 -91.0 -98.1 17.6 -40.2 1.4 -21.5 96 98 A G S < S+ 0 0 14 -3,-1.8 -2,-0.1 1,-0.3 2,-0.1 0.206 82.1 135.5 99.0 -18.0 -41.8 3.0 -18.4 97 99 A G - 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