==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=4-OCT-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 08-JUL-13 4BX5 . COMPND 2 MOLECULE: STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR M.FAIRHEAD,D.KRNDIJA,E.D.LOWE,M.HOWARTH . 480 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 20610.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 326 67.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 247 51.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 46 9.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 29 6.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 3 0 4 4 4 4 0 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 14 A E 0 0 181 0, 0.0 2,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 81.6 -25.5 5.5 11.0 2 15 A A + 0 0 55 3,-0.0 3,-0.2 0, 0.0 18,-0.1 0.491 360.0 159.3 -96.2 148.8 -22.7 5.7 10.5 3 16 A G + 0 0 15 1,-0.1 85,-0.0 -2,-0.0 0, 0.0 0.130 58.2 90.7-104.9 15.9 -21.5 2.2 11.2 4 17 A I + 0 0 3 16,-0.1 14,-0.2 78,-0.1 -1,-0.1 0.863 60.8 95.4 -77.8 -40.3 -18.3 2.7 9.2 5 18 A T S S+ 0 0 69 15,-0.2 2,-0.3 -3,-0.2 14,-0.2 -0.286 71.1 44.5 -54.4 132.3 -16.2 4.0 12.1 6 19 A G E S-A 18 0A 23 12,-2.8 12,-2.1 2,-0.0 2,-0.4 -0.927 93.5 -27.6 135.2-155.1 -14.1 1.2 13.7 7 20 A T E -A 17 0A 54 -2,-0.3 2,-0.4 10,-0.2 10,-0.2 -0.805 47.9-167.2-110.6 133.9 -11.9 -1.7 12.8 8 21 A W E -A 16 0A 1 8,-3.1 8,-2.4 -2,-0.4 2,-0.4 -0.943 5.3-153.3-128.0 142.2 -12.1 -3.7 9.6 9 22 A Y E -AB 15 117A 52 108,-2.7 108,-2.3 -2,-0.4 6,-0.2 -0.957 5.0-149.4-122.3 130.0 -10.6 -7.0 8.7 10 23 A A > - 0 0 2 4,-2.9 3,-1.3 -2,-0.4 106,-0.2 -0.517 32.3-105.8 -89.9 165.9 -9.7 -8.4 5.2 11 24 A Q T 3 S+ 0 0 125 104,-0.5 -1,-0.1 1,-0.3 105,-0.1 0.860 115.7 44.4 -59.8 -39.1 -9.7 -12.1 4.3 12 25 A L T 3 S- 0 0 134 21,-0.0 -1,-0.3 1,-0.0 21,-0.0 0.177 127.5 -84.4-101.5 17.8 -5.9 -12.5 4.2 13 26 A G S < S+ 0 0 42 -3,-1.3 -2,-0.1 1,-0.4 2,-0.1 0.272 79.6 135.3 108.0 -11.5 -5.1 -10.7 7.4 14 27 A D - 0 0 37 19,-0.1 -4,-2.9 18,-0.1 -1,-0.4 -0.411 33.1-169.1 -63.3 149.5 -4.8 -7.0 6.6 15 28 A T E -AC 9 31A 35 16,-2.1 16,-3.4 -6,-0.2 2,-0.3 -0.998 8.5-174.7-140.5 138.2 -6.6 -4.7 9.2 16 29 A F E -AC 8 30A 4 -8,-2.4 -8,-3.1 -2,-0.3 2,-0.4 -0.967 14.3-150.8-133.2 158.6 -7.5 -1.1 9.2 17 30 A I E +AC 7 29A 89 12,-2.4 12,-1.9 -2,-0.3 2,-0.3 -0.947 24.4 177.8-120.6 138.6 -9.0 1.3 11.7 18 31 A V E -AC 6 28A 3 -12,-2.1 -12,-2.8 -2,-0.4 2,-0.5 -0.996 25.3-148.9-143.4 146.6 -11.0 4.3 10.3 19 32 A T E - C 0 27A 79 8,-2.8 8,-1.7 -2,-0.3 2,-0.8 -0.988 14.7-150.6-110.7 124.5 -13.0 7.2 11.6 20 33 A A E - C 0 26A 14 -2,-0.5 6,-0.2 6,-0.2 -15,-0.2 -0.864 22.1-147.4 -93.4 104.1 -15.9 8.3 9.4 21 34 A G > - 0 0 18 4,-2.8 3,-1.9 -2,-0.8 25,-0.1 -0.292 21.2-115.4 -73.9 155.2 -16.2 12.1 10.1 22 35 A A T 3 S+ 0 0 104 1,-0.3 -1,-0.1 2,-0.1 26,-0.0 0.701 114.8 59.0 -64.5 -17.4 -19.5 13.9 10.0 23 36 A D T 3 S- 0 0 108 2,-0.1 -1,-0.3 22,-0.0 25,-0.1 0.220 127.4 -91.9 -93.9 10.7 -18.2 15.9 7.0 24 37 A G S < S+ 0 0 0 -3,-1.9 22,-2.1 1,-0.3 2,-0.2 0.459 79.7 142.7 93.6 4.9 -17.5 12.9 4.7 25 38 A A E - D 0 45A 22 20,-0.2 -4,-2.8 23,-0.0 2,-0.5 -0.548 35.5-161.9 -82.4 141.3 -13.9 12.3 5.8 26 39 A L E +CD 20 44A 4 18,-2.5 18,-2.4 -2,-0.2 2,-0.3 -0.991 21.4 165.4-112.3 126.2 -12.3 8.9 6.2 27 40 A T E +CD 19 43A 73 -8,-1.7 -8,-2.8 -2,-0.5 2,-0.3 -0.992 17.6 93.0-136.5 146.8 -9.1 9.0 8.2 28 41 A G E -CD 18 42A 19 14,-1.8 14,-2.3 -2,-0.3 2,-0.4 -0.984 60.3 -66.8 170.0-153.5 -7.2 6.1 9.8 29 42 A T E -CD 17 41A 56 -12,-1.9 -12,-2.4 -2,-0.3 2,-0.4 -0.997 26.5-153.6-140.6 135.9 -4.5 3.6 9.6 30 43 A Y E -CD 16 40A 3 10,-2.9 10,-2.5 -2,-0.4 2,-0.6 -0.935 7.4-159.9-114.0 129.7 -4.0 0.5 7.5 31 44 A E E -CD 15 39A 69 -16,-3.4 -16,-2.1 -2,-0.4 2,-0.4 -0.937 9.6-148.3-115.6 112.6 -1.9 -2.4 8.7 32 45 A A 0 0 25 6,-2.2 -18,-0.1 -2,-0.6 -19,-0.0 -0.643 360.0 360.0 -80.7 133.6 -0.6 -4.8 6.0 33 46 A A 0 0 97 -2,-0.4 -19,-0.1 -21,-0.0 -1,-0.1 -0.422 360.0 360.0 -63.1 360.0 -0.3 -8.4 7.1 34 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 35 49 A G > 0 0 67 0, 0.0 3,-1.8 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-168.8 4.2 -3.8 14.2 36 50 A A G > + 0 0 43 1,-0.3 3,-1.2 2,-0.2 33,-0.2 0.622 360.0 75.3 -69.7 -14.1 4.2 -0.1 13.3 37 51 A E G 3 S+ 0 0 97 1,-0.3 -1,-0.3 32,-0.1 32,-0.1 0.707 99.6 44.1 -69.5 -18.0 6.6 -1.0 10.5 38 52 A S G < S+ 0 0 22 -3,-1.8 -6,-2.2 31,-0.0 2,-0.4 0.182 93.7 103.3-113.4 18.5 3.6 -2.3 8.6 39 53 A R E < +D 31 0A 117 -3,-1.2 2,-0.3 -8,-0.2 -8,-0.2 -0.847 45.4 179.4-107.8 134.7 1.2 0.6 9.4 40 54 A Y E -D 30 0A 24 -10,-2.5 -10,-2.9 -2,-0.4 2,-0.2 -0.954 33.8 -98.0-133.7 149.1 0.3 3.3 6.9 41 55 A V E -D 29 0A 65 25,-0.4 23,-0.5 -2,-0.3 2,-0.4 -0.466 34.6-160.5 -66.4 135.5 -1.8 6.4 6.8 42 56 A L E +DE 28 63A 1 -14,-2.3 -14,-1.8 -2,-0.2 2,-0.3 -0.967 14.4 171.4-115.0 134.8 -5.3 5.9 5.2 43 57 A T E +DE 27 62A 21 19,-1.9 19,-2.2 -2,-0.4 2,-0.2 -0.988 14.0 121.6-147.2 133.7 -7.3 8.8 3.9 44 58 A G E -DE 26 61A 0 -18,-2.4 -18,-2.5 -2,-0.3 2,-0.3 -0.830 43.8-100.0-162.1-160.5 -10.5 9.0 1.9 45 59 A R E -DE 25 60A 22 15,-1.9 15,-2.0 -20,-0.3 2,-0.3 -0.971 22.7-172.7-141.0 151.4 -14.1 10.2 1.5 46 60 A Y E - E 0 59A 16 -22,-2.1 2,-0.7 -2,-0.3 13,-0.2 -0.959 40.6 -90.7-141.7 160.3 -17.6 8.6 1.9 47 61 A D - 0 0 2 11,-2.3 -23,-0.1 -2,-0.3 149,-0.0 -0.658 35.1-168.3 -69.0 106.9 -21.2 9.6 1.3 48 62 A S S S+ 0 0 35 -2,-0.7 -1,-0.2 1,-0.3 -24,-0.1 0.505 82.5 44.1 -80.6 -6.7 -22.2 11.2 4.6 49 63 A A S S+ 0 0 45 -25,-0.0 -1,-0.3 -3,-0.0 145,-0.1 -0.487 82.4 175.8-133.4 65.5 -25.9 11.3 3.6 50 64 A P - 0 0 17 0, 0.0 7,-0.1 0, 0.0 2,-0.1 -0.156 36.8 -93.6 -70.5 162.6 -26.6 7.8 2.0 51 65 A A - 0 0 27 5,-0.3 2,-0.3 1,-0.1 3,-0.2 -0.398 21.2-145.0 -68.0 150.5 -29.9 6.6 0.9 52 66 A T S S+ 0 0 139 1,-0.1 -1,-0.1 -2,-0.1 4,-0.1 -0.577 73.3 101.5-111.5 63.5 -31.9 4.5 3.4 53 67 A D S S- 0 0 108 -2,-0.3 -1,-0.1 2,-0.2 3,-0.1 0.083 106.7 -84.2-130.7 15.3 -33.4 2.3 0.7 54 68 A G S S+ 0 0 65 1,-0.3 169,-0.3 -3,-0.2 2,-0.3 0.388 91.7 124.6 93.1 -4.8 -31.2 -0.9 1.0 55 69 A S - 0 0 20 167,-0.1 -1,-0.3 1,-0.1 167,-0.3 -0.642 63.4-103.7 -89.1 151.5 -28.4 0.4 -1.1 56 70 A G - 0 0 8 165,-2.2 2,-0.6 -2,-0.3 -5,-0.3 -0.047 30.3-106.3 -66.0 171.4 -24.8 0.6 0.1 57 71 A T E - F 0 82A 15 25,-2.5 25,-2.4 -7,-0.1 -1,-0.1 -0.888 37.4-128.4-103.7 113.4 -22.9 3.7 1.2 58 72 A A E + F 0 81A 1 -2,-0.6 -11,-2.3 23,-0.2 2,-0.3 -0.346 41.1 151.5 -67.3 146.1 -20.4 4.7 -1.4 59 73 A L E -EF 46 80A 0 21,-2.2 21,-2.3 -13,-0.2 2,-0.3 -0.959 21.8-171.9-161.3 169.9 -16.8 5.3 -0.3 60 74 A G E -EF 45 79A 0 -15,-2.0 -15,-1.9 -2,-0.3 2,-0.3 -0.957 5.0-179.1-164.1 156.2 -13.2 5.3 -1.3 61 75 A W E -EF 44 78A 3 17,-2.0 17,-2.6 -2,-0.3 2,-0.4 -0.987 14.3-138.9-155.2 165.7 -9.7 5.7 0.2 62 76 A T E -EF 43 77A 0 -19,-2.2 -19,-1.9 -2,-0.3 2,-0.4 -0.959 6.7-169.7-124.6 144.2 -6.0 5.7 -0.6 63 77 A V E -EF 42 76A 0 13,-2.5 13,-2.5 -2,-0.4 2,-0.5 -0.988 11.2-155.9-127.6 122.8 -2.9 4.3 1.1 64 78 A A E - F 0 75A 3 -23,-0.5 2,-1.8 -2,-0.4 11,-0.3 -0.868 26.0-127.6 -91.8 127.1 0.5 5.4 -0.2 65 79 A W E S+ 0 0 33 9,-2.5 7,-2.4 -2,-0.5 2,-0.4 -0.249 73.9 105.3 -80.9 47.2 2.8 2.6 0.8 66 80 A K E + F 0 71A 54 -2,-1.8 -25,-0.4 5,-0.2 5,-0.2 -0.991 34.8 152.5-119.5 136.0 5.4 4.8 2.5 67 81 A N - 0 0 21 3,-2.5 -27,-0.1 -2,-0.4 -30,-0.1 -0.677 64.2 -75.2-138.5-163.4 5.8 5.1 6.2 68 82 A N S S+ 0 0 150 -2,-0.2 -31,-0.1 1,-0.2 3,-0.1 0.642 129.0 35.0 -71.4 -11.8 8.8 6.0 8.4 69 83 A Y S S+ 0 0 130 1,-0.2 2,-0.3 -33,-0.2 -1,-0.2 0.643 120.4 19.6-114.3 -26.1 10.4 2.6 7.9 70 84 A R - 0 0 118 -33,-0.0 -3,-2.5 0, 0.0 2,-0.3 -0.938 48.5-164.6-150.6 160.8 9.6 1.4 4.4 71 85 A N E +F 66 0A 46 -2,-0.3 -5,-0.2 -5,-0.2 -6,-0.0 -0.802 9.8 179.9-158.5 115.0 8.5 2.6 0.9 72 86 A A E - 0 0 60 -7,-2.4 27,-0.2 -2,-0.3 -6,-0.1 0.377 45.2-125.4 -93.3 -2.4 7.2 0.5 -2.0 73 87 A H E S+ 0 0 60 -8,-0.3 26,-2.6 1,-0.2 2,-0.3 0.945 76.2 96.1 54.8 53.4 6.8 3.6 -4.3 74 88 A S E - G 0 98A 8 24,-0.3 -9,-2.5 105,-0.1 2,-0.3 -0.963 56.3-149.8-160.2 171.3 3.2 2.7 -4.9 75 89 A A E -FG 64 97A 0 22,-2.1 22,-2.5 -2,-0.3 2,-0.4 -0.985 6.1-149.2-154.2 139.5 -0.3 3.5 -3.8 76 90 A T E -FG 63 96A 6 -13,-2.5 -13,-2.5 -2,-0.3 2,-0.4 -0.915 6.8-165.1-108.8 135.6 -3.6 1.6 -3.6 77 91 A T E -FG 62 95A 0 18,-2.3 18,-2.3 -2,-0.4 2,-0.4 -0.992 6.1-158.3-114.3 136.4 -7.0 3.1 -3.9 78 92 A W E -FG 61 94A 7 -17,-2.6 -17,-2.0 -2,-0.4 2,-0.4 -0.949 7.1-172.5-112.7 128.4 -10.1 1.2 -2.9 79 93 A S E +FG 60 93A 0 14,-3.0 14,-2.4 -2,-0.4 2,-0.3 -0.986 33.8 100.0-120.0 128.9 -13.5 2.1 -4.3 80 94 A G E -FG 59 92A 2 -21,-2.3 -21,-2.2 -2,-0.4 2,-0.3 -0.882 57.3 -82.2-171.7-157.6 -16.6 0.4 -2.9 81 95 A Q E -FG 58 91A 9 10,-2.0 10,-2.6 -2,-0.3 2,-0.4 -1.000 22.7-129.9-143.1 144.5 -19.6 0.5 -0.7 82 96 A Y E -FG 57 90A 31 -25,-2.4 -25,-2.5 -2,-0.3 2,-0.5 -0.780 20.5-162.4 -88.0 131.1 -20.4 -0.2 3.0 83 97 A V E - 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