==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER MEMBRANE PROTEIN, PROTEIN TRANSPORT 18-JAN-08 3BZO . COMPND 2 MOLECULE: ESCU; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR R.ZARIVACH,W.DENG,M.VUCKOVIC,H.B.FELISE,H.V.NGUYEN,S.I.MILLE . 99 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 6341.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 68 68.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 4 4.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 15 15.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 5 5.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 7 7.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 34 34.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 246 A S 0 0 185 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 154.9 -7.6 -8.9 -25.9 2 247 A G > + 0 0 36 3,-0.0 4,-2.5 4,-0.0 5,-0.3 0.269 360.0 116.9 108.1 -9.3 -10.8 -10.1 -24.2 3 248 A S H > S+ 0 0 80 1,-0.2 4,-2.3 2,-0.2 5,-0.2 0.887 77.8 51.4 -59.5 -40.9 -10.1 -13.8 -23.8 4 249 A L H > S+ 0 0 26 1,-0.2 4,-2.4 2,-0.2 -1,-0.2 0.961 112.2 45.2 -60.2 -51.9 -10.2 -13.6 -20.0 5 250 A A H > S+ 0 0 22 1,-0.2 4,-2.7 2,-0.2 -2,-0.2 0.883 112.9 50.3 -62.8 -40.2 -13.5 -11.8 -19.9 6 251 A N H X S+ 0 0 105 -4,-2.5 4,-1.5 2,-0.2 -1,-0.2 0.876 110.4 50.7 -67.5 -36.1 -15.1 -14.2 -22.5 7 252 A N H X S+ 0 0 39 -4,-2.3 4,-0.9 -5,-0.3 -2,-0.2 0.943 112.1 45.8 -63.7 -46.2 -13.9 -17.2 -20.6 8 253 A I H >< S+ 0 0 0 -4,-2.4 3,-1.1 1,-0.2 -2,-0.2 0.935 108.2 57.9 -62.4 -43.3 -15.4 -15.8 -17.3 9 254 A K H 3< S+ 0 0 131 -4,-2.7 -1,-0.2 1,-0.3 -2,-0.2 0.872 113.4 37.9 -56.4 -37.3 -18.7 -14.9 -19.1 10 255 A K H 3< S+ 0 0 98 -4,-1.5 -1,-0.3 -5,-0.1 -2,-0.2 0.543 94.4 115.6 -90.9 -6.8 -19.3 -18.5 -20.3 11 256 A S << - 0 0 10 -3,-1.1 15,-0.2 -4,-0.9 3,-0.1 -0.132 57.7-144.4 -71.1 164.5 -18.0 -20.2 -17.1 12 257 A T S S- 0 0 25 13,-2.4 2,-0.3 1,-0.3 14,-0.2 0.841 72.0 -11.2 -95.8 -44.7 -20.1 -22.3 -14.8 13 258 A V E -A 25 0A 0 12,-1.8 12,-2.9 45,-0.1 2,-0.4 -0.981 52.8-140.1-160.4 147.4 -18.7 -21.5 -11.3 14 259 A I E -Ab 24 60A 0 45,-2.5 47,-2.9 -2,-0.3 2,-0.5 -0.952 13.6-158.7-110.3 131.3 -15.7 -19.8 -9.7 15 260 A V E +Ab 23 61A 0 8,-2.7 8,-2.5 -2,-0.4 2,-0.3 -0.954 19.4 175.8-107.2 132.2 -14.1 -21.3 -6.6 16 261 A K E Ab 22 62A 39 45,-2.4 47,-2.6 -2,-0.5 51,-0.3 -0.860 360.0 360.0-130.8 160.5 -12.1 -18.9 -4.4 17 262 A N 0 0 42 4,-1.4 47,-0.1 -2,-0.3 3,-0.1 -0.183 360.0 360.0 -95.2 360.0 -10.2 -19.0 -1.2 18 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 19 264 B T 0 0 121 0, 0.0 2,-0.2 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 113.4 -2.6 -15.0 -1.4 20 265 B H - 0 0 98 -3,-0.1 23,-2.1 -4,-0.0 2,-0.3 -0.506 360.0-171.1 -77.3 140.1 -5.9 -14.7 -3.2 21 266 B I E - C 0 42A 15 21,-0.2 -4,-1.4 -2,-0.2 2,-0.3 -0.977 9.8-177.1-131.8 140.8 -6.8 -17.6 -5.6 22 267 B A E -AC 16 41A 0 19,-2.2 19,-2.1 -2,-0.3 2,-0.5 -0.989 5.1-167.4-141.1 135.4 -9.7 -18.0 -8.1 23 268 B I E -AC 15 40A 0 -8,-2.5 -8,-2.7 -2,-0.3 2,-0.6 -0.989 10.0-152.2-124.2 122.4 -10.4 -21.0 -10.2 24 269 B C E -AC 14 39A 0 15,-3.1 14,-2.0 -2,-0.5 15,-1.1 -0.874 13.1-163.8-102.2 116.1 -12.9 -20.8 -13.1 25 270 B L E -AC 13 37A 1 -12,-2.9 -13,-2.4 -2,-0.6 -12,-1.8 -0.834 4.7-168.8-104.3 138.5 -14.7 -24.0 -13.9 26 271 B Y E + C 0 36A 19 10,-2.8 10,-2.6 -2,-0.4 2,-0.4 -0.988 10.8 167.6-126.7 125.4 -16.5 -24.7 -17.1 27 272 B Y + 0 0 76 -2,-0.4 2,-0.4 8,-0.2 -2,-0.0 -0.964 2.7 169.0-139.3 125.2 -18.8 -27.6 -17.8 28 273 B K >> - 0 0 119 -2,-0.4 3,-2.0 6,-0.2 4,-1.5 -0.946 36.0-123.1-134.2 114.6 -21.2 -28.0 -20.7 29 274 B L T 34 S+ 0 0 148 -2,-0.4 -2,-0.0 1,-0.3 5,-0.0 -0.295 99.2 21.7 -56.4 134.3 -22.8 -31.3 -21.3 30 275 B G T 34 S+ 0 0 81 2,-0.3 -1,-0.3 1,-0.1 0, 0.0 -0.060 127.1 51.1 96.9 -31.4 -22.0 -32.5 -24.9 31 276 B E T <4 S+ 0 0 105 -3,-2.0 -2,-0.2 1,-0.3 -1,-0.1 0.866 104.0 44.7-100.2 -69.2 -18.9 -30.3 -25.2 32 277 B T < - 0 0 6 -4,-1.5 -2,-0.3 1,-0.1 -1,-0.3 -0.684 63.1-157.4 -83.8 121.2 -16.6 -30.8 -22.2 33 278 B P S S+ 0 0 92 0, 0.0 -1,-0.1 0, 0.0 -6,-0.0 0.770 84.3 10.0 -68.7 -25.4 -16.2 -34.5 -21.3 34 279 B L S S- 0 0 59 -8,-0.1 -6,-0.2 -6,-0.1 -8,-0.1 -0.950 104.7 -74.5-141.2 157.5 -15.2 -33.4 -17.8 35 280 B P - 0 0 16 0, 0.0 45,-2.4 0, 0.0 2,-0.4 -0.361 54.7-146.5 -54.7 141.4 -15.3 -30.1 -15.9 36 281 B L E -CD 26 79A 28 -10,-2.6 -10,-2.8 43,-0.2 2,-1.0 -0.916 14.2-135.6-118.1 135.9 -12.5 -27.9 -17.3 37 282 B V E +C 25 0A 0 41,-2.5 40,-3.4 -2,-0.4 41,-0.5 -0.830 32.8 170.0 -87.6 106.3 -10.4 -25.3 -15.5 38 283 B I E + 0 0 14 -14,-2.0 2,-0.3 -2,-1.0 -1,-0.2 0.720 60.6 13.8 -97.5 -19.8 -10.5 -22.6 -18.1 39 284 B E E -C 24 0A 1 -15,-1.1 -15,-3.1 -3,-0.1 2,-0.3 -0.990 59.4-179.5-149.7 147.4 -9.0 -19.8 -16.1 40 285 B T E -C 23 0A 24 -2,-0.3 2,-0.3 -17,-0.2 -17,-0.2 -0.952 5.8-177.9-136.8 162.2 -7.1 -19.4 -12.9 41 286 B G E -C 22 0A 2 -19,-2.1 -19,-2.2 -2,-0.3 2,-0.3 -0.985 12.4-163.7-156.7 150.6 -5.6 -16.4 -11.1 42 287 B K E > -C 21 0A 93 -2,-0.3 3,-1.5 -21,-0.2 4,-0.4 -0.930 66.2 -16.4-124.7 155.6 -3.6 -15.4 -8.0 43 288 B D T 3> S+ 0 0 83 -23,-2.1 4,-1.9 -2,-0.3 3,-0.4 -0.239 135.6 5.9 56.5-129.1 -3.3 -12.0 -6.4 44 289 B A H 3> S+ 0 0 76 1,-0.3 4,-2.0 2,-0.2 -1,-0.3 0.859 134.1 53.0 -56.8 -37.3 -4.4 -9.2 -8.8 45 290 B K H <> S+ 0 0 76 -3,-1.5 4,-2.5 1,-0.2 -1,-0.3 0.894 106.2 52.6 -68.1 -38.0 -5.6 -11.6 -11.4 46 291 B A H > S+ 0 0 0 -4,-0.4 4,-2.1 -3,-0.4 -1,-0.2 0.919 108.6 50.9 -60.0 -42.5 -7.8 -13.4 -8.8 47 292 B L H X S+ 0 0 82 -4,-1.9 4,-2.1 1,-0.2 -2,-0.2 0.909 110.8 49.1 -62.3 -39.5 -9.4 -10.1 -7.8 48 293 B Q H X S+ 0 0 95 -4,-2.0 4,-2.4 1,-0.2 -1,-0.2 0.906 107.4 53.6 -69.0 -41.0 -10.1 -9.3 -11.5 49 294 B I H X S+ 0 0 0 -4,-2.5 4,-2.6 1,-0.2 -1,-0.2 0.918 109.3 49.6 -57.6 -42.8 -11.7 -12.8 -12.1 50 295 B I H X S+ 0 0 42 -4,-2.1 4,-2.2 2,-0.2 -1,-0.2 0.895 109.3 51.0 -66.8 -39.5 -14.0 -12.2 -9.1 51 296 B K H X S+ 0 0 142 -4,-2.1 4,-2.1 2,-0.2 -1,-0.2 0.889 112.1 47.5 -62.4 -40.7 -15.0 -8.7 -10.5 52 297 B L H X S+ 0 0 25 -4,-2.4 4,-2.3 2,-0.2 -2,-0.2 0.883 107.5 56.4 -68.4 -38.6 -15.7 -10.2 -13.8 53 298 B A H <>S+ 0 0 0 -4,-2.6 5,-2.7 2,-0.2 4,-0.5 0.926 108.2 47.7 -55.8 -45.8 -17.7 -13.0 -12.2 54 299 B E H ><5S+ 0 0 153 -4,-2.2 3,-1.0 1,-0.2 -2,-0.2 0.930 110.8 52.2 -62.1 -44.3 -19.9 -10.3 -10.5 55 300 B L H 3<5S+ 0 0 111 -4,-2.1 -1,-0.2 1,-0.2 -2,-0.2 0.878 117.6 36.5 -57.5 -41.4 -20.3 -8.5 -13.8 56 301 B Y T 3<5S- 0 0 68 -4,-2.3 -1,-0.2 -5,-0.1 -2,-0.2 0.309 110.6-118.7 -99.1 10.7 -21.4 -11.7 -15.6 57 302 B D T < 5 + 0 0 141 -3,-1.0 -3,-0.2 -4,-0.5 -4,-0.1 0.888 52.9 164.7 53.7 44.8 -23.4 -13.1 -12.6 58 303 B I < - 0 0 19 -5,-2.7 -1,-0.2 -6,-0.2 -45,-0.1 -0.800 44.8-108.0 -90.5 127.7 -21.2 -16.2 -12.4 59 304 B P - 0 0 52 0, 0.0 -45,-2.5 0, 0.0 2,-0.5 -0.328 33.2-159.2 -56.0 133.0 -21.7 -18.0 -9.0 60 305 B V E -b 14 0A 57 -47,-0.2 2,-0.5 -45,-0.0 -45,-0.2 -0.987 7.0-169.7-117.8 123.1 -18.6 -17.6 -6.8 61 306 B I E -b 15 0A 34 -47,-2.9 -45,-2.4 -2,-0.5 2,-0.6 -0.949 15.1-142.0-116.0 128.6 -18.2 -20.1 -4.0 62 307 B E E +b 16 0A 121 -2,-0.5 2,-0.3 -47,-0.2 -45,-0.2 -0.801 37.5 147.8 -90.5 120.7 -15.6 -19.7 -1.2 63 308 B D > - 0 0 66 -47,-2.6 4,-3.0 -2,-0.6 5,-0.2 -0.780 24.3-169.2-153.1 96.8 -14.0 -23.1 -0.3 64 309 B I H > S+ 0 0 57 -2,-0.3 4,-2.6 1,-0.2 5,-0.3 0.937 83.4 46.0 -68.4 -49.4 -10.4 -22.6 0.8 65 310 B P H > S+ 0 0 89 0, 0.0 4,-1.9 0, 0.0 -1,-0.2 0.923 117.7 45.0 -58.4 -40.5 -9.2 -26.2 0.9 66 311 B L H > S+ 0 0 18 2,-0.2 4,-3.0 1,-0.2 5,-0.3 0.927 112.9 50.5 -67.4 -45.1 -10.8 -26.9 -2.5 67 312 B A H X S+ 0 0 0 -4,-3.0 4,-2.5 -51,-0.3 5,-0.2 0.939 112.1 46.8 -60.1 -46.1 -9.5 -23.7 -4.1 68 313 B R H X S+ 0 0 142 -4,-2.6 4,-2.0 -5,-0.2 -1,-0.2 0.910 114.7 47.1 -61.5 -41.7 -6.0 -24.3 -2.9 69 314 B S H X S+ 0 0 45 -4,-1.9 4,-2.4 -5,-0.3 5,-0.3 0.920 113.2 47.0 -69.5 -45.7 -6.0 -27.9 -4.1 70 315 B L H X S+ 0 0 0 -4,-3.0 4,-2.6 1,-0.2 -1,-0.2 0.921 111.1 53.3 -57.6 -45.4 -7.5 -27.1 -7.5 71 316 B Y H < S+ 0 0 60 -4,-2.5 -2,-0.2 -5,-0.3 -1,-0.2 0.900 114.5 40.9 -58.9 -41.7 -5.0 -24.3 -8.0 72 317 B K H < S+ 0 0 162 -4,-2.0 -1,-0.2 -5,-0.2 -2,-0.2 0.892 126.1 31.0 -73.7 -37.0 -2.1 -26.6 -7.2 73 318 B N H < S+ 0 0 85 -4,-2.4 2,-0.7 1,-0.2 -2,-0.2 0.822 111.7 53.7 -96.6 -35.2 -3.3 -29.6 -9.2 74 319 B I S < S- 0 0 2 -4,-2.6 -1,-0.2 -5,-0.3 3,-0.1 -0.893 71.0-159.0-115.6 104.1 -5.3 -28.5 -12.2 75 320 B H > - 0 0 108 -2,-0.7 3,-2.3 1,-0.2 -38,-0.3 -0.270 39.6 -69.3 -80.4 162.4 -3.7 -26.0 -14.4 76 321 B K T 3 S+ 0 0 109 1,-0.3 -38,-0.2 -5,-0.1 -1,-0.2 -0.301 123.9 18.3 -50.8 128.8 -5.3 -23.7 -16.9 77 322 B G T 3 S+ 0 0 51 -40,-3.4 2,-0.3 1,-0.3 -1,-0.3 0.454 99.7 118.9 87.4 3.1 -6.8 -25.7 -19.8 78 323 B Q < - 0 0 87 -3,-2.3 -41,-2.5 -41,-0.5 -1,-0.3 -0.781 63.3-122.7-101.5 143.3 -6.8 -29.0 -17.9 79 324 B Y B -D 36 0A 102 -2,-0.3 -43,-0.2 -43,-0.2 -4,-0.1 -0.530 39.6 -97.3 -73.4 150.0 -9.8 -31.2 -17.0 80 325 B I - 0 0 4 -45,-2.4 2,-0.2 -2,-0.2 -1,-0.1 -0.260 40.4-131.7 -64.0 157.3 -10.3 -32.0 -13.4 81 326 B T > - 0 0 68 -3,-0.1 3,-2.5 -47,-0.0 4,-0.5 -0.613 32.3 -82.4-112.0 173.7 -9.0 -35.3 -12.1 82 327 B E G > S+ 0 0 154 1,-0.3 3,-1.1 -2,-0.2 4,-0.5 0.726 120.7 60.7 -53.3 -37.4 -10.6 -38.0 -10.0 83 328 B D G 3 S+ 0 0 125 1,-0.2 -1,-0.3 2,-0.1 3,-0.1 0.734 111.8 42.4 -61.5 -22.6 -10.0 -36.5 -6.6 84 329 B F G <> S+ 0 0 8 -3,-2.5 4,-2.4 1,-0.1 5,-0.4 0.430 84.3 97.8-102.0 -7.3 -12.1 -33.5 -7.6 85 330 B F H <> S+ 0 0 91 -3,-1.1 4,-1.8 -4,-0.5 -2,-0.1 0.929 92.5 33.7 -55.0 -51.3 -15.0 -35.3 -9.4 86 331 B E H > S+ 0 0 111 -4,-0.5 4,-2.3 2,-0.2 5,-0.2 0.945 116.9 51.3 -75.9 -46.1 -17.4 -35.2 -6.6 87 332 B P H > S+ 0 0 44 0, 0.0 4,-1.3 0, 0.0 -1,-0.2 0.901 115.2 44.9 -57.4 -37.5 -16.5 -31.9 -4.9 88 333 B V H X S+ 0 0 0 -4,-2.4 4,-2.5 2,-0.2 -2,-0.2 0.880 108.2 57.2 -73.3 -35.1 -16.8 -30.2 -8.2 89 334 B A H X S+ 0 0 26 -4,-1.8 4,-2.8 -5,-0.4 -1,-0.2 0.909 104.3 53.2 -57.2 -41.6 -20.0 -32.0 -9.0 90 335 B Q H X S+ 0 0 115 -4,-2.3 4,-1.4 1,-0.2 -1,-0.2 0.879 109.3 48.8 -62.9 -37.7 -21.5 -30.5 -5.8 91 336 B L H X S+ 0 0 9 -4,-1.3 4,-2.1 -5,-0.2 -1,-0.2 0.904 111.0 50.0 -65.3 -44.4 -20.4 -27.0 -7.0 92 337 B I H X S+ 0 0 24 -4,-2.5 4,-2.8 1,-0.2 -2,-0.2 0.936 105.9 56.4 -63.1 -42.9 -22.0 -27.6 -10.4 93 338 B R H < S+ 0 0 186 -4,-2.8 -1,-0.2 1,-0.2 -2,-0.2 0.879 114.7 38.1 -57.5 -38.8 -25.2 -28.7 -8.8 94 339 B I H < S+ 0 0 123 -4,-1.4 -1,-0.2 -5,-0.2 -2,-0.2 0.790 121.9 43.3 -81.7 -26.1 -25.6 -25.5 -6.8 95 340 B A H < S+ 0 0 10 -4,-2.1 -2,-0.2 -5,-0.1 -3,-0.2 0.713 81.9 115.3 -94.8 -22.0 -24.2 -23.2 -9.5 96 341 B I < - 0 0 74 -4,-2.8 2,-0.7 -5,-0.2 3,-0.0 -0.177 63.8-132.8 -57.2 136.9 -26.0 -24.5 -12.6 97 342 B D - 0 0 133 1,-0.1 -1,-0.1 2,-0.1 -2,-0.1 -0.846 11.9-161.0 -89.5 116.7 -28.5 -22.1 -14.2 98 343 B L - 0 0 114 -2,-0.7 -1,-0.1 2,-0.2 -2,-0.1 0.638 29.6-132.9 -78.5 -13.7 -31.6 -24.2 -14.8 99 344 B D 0 0 145 1,-0.2 -2,-0.1 -3,-0.0 -1,-0.0 0.761 360.0 360.0 74.0 28.4 -32.7 -21.5 -17.3 100 345 B Y 0 0 261 0, 0.0 -1,-0.2 0, 0.0 -2,-0.2 -0.959 360.0 360.0-138.9 360.0 -36.3 -21.0 -16.3