==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 03-MAR-08 3CFA . COMPND 2 MOLECULE: GFP-LIKE FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: ANEMONIA SULCATA; . AUTHOR G.S.KACHALOVA,S.GUNDEL,J.WIEDENMANN,H.D.BARTUNIK . 896 10 2 0 2 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32632.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 650 72.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 406 45.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 71 7.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 79 8.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 31 3.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 0 0 0 3 0 8 5 8 1 14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 5 L L 0 0 76 0, 0.0 2,-0.3 0, 0.0 106,-0.0 0.000 360.0 360.0 360.0-172.3 -3.1 1.7 0.3 2 6 L T - 0 0 91 105,-0.1 3,-0.2 1,-0.0 29,-0.1 -0.801 360.0-104.3-112.6 161.9 -6.1 3.5 -1.2 3 7 L E S S+ 0 0 131 -2,-0.3 2,-0.2 1,-0.2 29,-0.1 0.443 113.0 39.7 -73.5 2.1 -6.5 7.3 -1.3 4 8 L T S S+ 0 0 74 25,-0.2 -1,-0.2 26,-0.0 25,-0.2 -0.698 75.8 178.5-145.6 95.5 -9.1 7.3 1.5 5 9 L M E -A 28 0A 7 23,-2.1 23,-2.1 -3,-0.2 2,-0.2 -0.827 17.2-144.3-100.2 130.1 -8.3 4.9 4.3 6 10 L P E -A 27 0A 36 0, 0.0 103,-2.2 0, 0.0 2,-0.3 -0.549 18.6-169.2 -83.8 157.6 -10.4 4.5 7.4 7 11 L F E -Ab 26 109A 1 19,-2.7 19,-2.3 101,-0.2 2,-0.3 -0.991 12.5-154.8-145.0 154.3 -8.9 3.7 10.8 8 12 L R E -Ab 25 110A 92 101,-2.8 103,-3.4 -2,-0.3 2,-0.4 -0.977 13.0-163.2-129.6 147.2 -10.0 2.7 14.3 9 13 L M E -Ab 24 111A 4 15,-2.5 15,-2.3 -2,-0.3 2,-0.4 -0.970 12.9-171.7-134.1 141.0 -8.3 3.3 17.7 10 14 L T E -Ab 23 112A 63 101,-1.8 103,-2.6 -2,-0.4 2,-0.4 -0.987 15.5-175.3-123.9 143.5 -8.4 2.0 21.2 11 15 L M E -Ab 22 113A 5 11,-2.4 11,-2.4 -2,-0.4 2,-0.4 -0.999 10.1-179.0-142.2 134.0 -6.4 3.7 24.0 12 16 L E E +Ab 21 114A 94 101,-2.1 103,-2.2 -2,-0.4 2,-0.3 -0.973 22.6 138.5-126.0 146.5 -5.8 3.0 27.7 13 17 L G E -Ab 20 115A 7 7,-2.1 7,-2.3 -2,-0.4 2,-0.4 -0.997 41.5-130.3-170.1 175.1 -3.8 5.0 30.1 14 18 L T E -Ab 19 116A 3 101,-1.4 103,-3.2 -2,-0.3 2,-0.4 -0.992 19.8-176.7-136.7 127.2 -3.1 6.6 33.5 15 19 L V E > S-Ab 18 117A 0 3,-2.2 3,-2.3 -2,-0.4 103,-0.2 -0.988 76.8 -19.9-127.2 120.8 -2.1 10.3 33.8 16 20 L N T 3 S- 0 0 12 101,-1.9 102,-0.1 -2,-0.4 3,-0.1 0.852 130.8 -48.9 50.3 38.9 -1.3 11.7 37.3 17 21 L G T 3 S+ 0 0 24 100,-0.4 2,-0.6 1,-0.2 -1,-0.3 0.299 110.9 124.3 87.9 -9.5 -3.2 8.7 38.8 18 22 L H E < -A 15 0A 78 -3,-2.3 -3,-2.2 30,-0.0 2,-0.3 -0.778 49.8-150.8 -88.9 117.2 -6.3 9.2 36.7 19 23 L H E +A 14 0A 94 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.679 25.2 163.7 -84.3 140.0 -7.2 6.0 34.7 20 24 L F E -A 13 0A 4 -7,-2.3 -7,-2.1 -2,-0.3 2,-0.3 -0.973 25.8-153.3-151.2 161.7 -9.0 6.5 31.4 21 25 L K E -AC 12 43A 60 22,-2.1 21,-1.9 -2,-0.3 22,-1.6 -0.995 14.4-173.0-137.4 140.5 -10.0 4.7 28.1 22 26 L C E -AC 11 41A 2 -11,-2.4 -11,-2.4 -2,-0.3 2,-0.3 -0.943 9.5-159.1-129.6 151.9 -10.6 6.3 24.7 23 27 L T E -AC 10 40A 46 17,-2.4 17,-2.8 -2,-0.3 2,-0.3 -0.926 13.7-168.4-121.7 156.6 -11.9 5.0 21.4 24 28 L G E -AC 9 39A 10 -15,-2.3 -15,-2.5 -2,-0.3 2,-0.3 -0.987 11.3-177.3-144.4 152.9 -11.4 6.7 18.0 25 29 L K E +AC 8 38A 118 13,-2.1 13,-2.7 -2,-0.3 2,-0.3 -0.971 24.2 124.3-150.4 134.4 -12.6 6.5 14.5 26 30 L G E -AC 7 37A 16 -19,-2.3 -19,-2.7 -2,-0.3 2,-0.3 -0.937 35.7-130.9-165.0-171.7 -11.6 8.4 11.4 27 31 L E E +AC 6 36A 110 9,-1.8 9,-2.4 -21,-0.3 2,-0.3 -0.978 21.1 171.8-151.6 159.6 -10.3 8.4 7.8 28 32 L G E -AC 5 35A 7 -23,-2.1 -23,-2.1 -2,-0.3 7,-0.3 -0.960 41.2 -95.0-158.1 176.1 -7.7 10.2 5.8 29 33 L N E >> - C 0 34A 19 5,-2.4 4,-1.8 -2,-0.3 5,-1.2 -0.883 32.9-164.1-100.3 101.5 -5.8 10.5 2.5 30 34 L P T 45S+ 0 0 0 0, 0.0 34,-1.0 0, 0.0 -1,-0.2 0.850 87.8 38.5 -57.8 -40.9 -2.6 8.4 3.0 31 35 L F T 45S+ 0 0 106 1,-0.2 35,-0.2 32,-0.1 -28,-0.1 0.707 118.4 48.2 -85.4 -21.3 -0.7 9.8 0.0 32 36 L E T 45S- 0 0 109 -3,-0.3 33,-0.4 2,-0.2 -1,-0.2 0.590 105.0-129.6 -91.9 -14.3 -1.9 13.4 0.5 33 37 L G T <5 + 0 0 1 -4,-1.8 177,-2.2 31,-0.3 2,-0.3 0.748 62.1 125.5 74.5 28.5 -1.1 13.3 4.3 34 38 L T E < +CD 29 209A 40 -5,-1.2 -5,-2.4 175,-0.2 2,-0.3 -0.900 28.5 164.8-119.0 147.8 -4.4 14.6 5.6 35 39 L Q E -CD 28 208A 10 173,-1.9 173,-2.3 -2,-0.3 2,-0.3 -0.992 19.5-161.1-160.0 154.5 -6.7 12.9 8.2 36 40 L D E +CD 27 207A 69 -9,-2.4 -9,-1.8 -2,-0.3 2,-0.3 -0.984 15.8 174.4-135.8 144.1 -9.7 13.5 10.5 37 41 L M E -CD 26 206A 10 169,-2.0 169,-2.6 -2,-0.3 2,-0.4 -0.990 24.7-138.3-149.2 156.9 -10.8 11.5 13.6 38 42 L K E -CD 25 205A 142 -13,-2.7 -13,-2.1 -2,-0.3 2,-0.4 -0.949 20.7-162.7-111.6 135.1 -13.4 11.5 16.4 39 43 L I E -CD 24 204A 1 165,-2.8 165,-2.6 -2,-0.4 2,-0.4 -0.976 2.9-167.0-120.7 132.5 -12.2 10.5 19.9 40 44 L E E -CD 23 203A 71 -17,-2.8 -17,-2.4 -2,-0.4 2,-0.8 -0.980 19.6-137.1-118.8 132.6 -14.6 9.5 22.7 41 45 L V E +C 22 0A 8 161,-2.6 -19,-0.3 -2,-0.4 4,-0.1 -0.789 27.8 173.4 -83.0 111.4 -13.6 9.1 26.3 42 46 L I E + 0 0 80 -21,-1.9 2,-0.3 -2,-0.8 -20,-0.2 0.629 64.2 27.3 -99.0 -18.0 -15.4 5.9 27.3 43 47 L E E S+C 21 0A 95 -22,-1.6 -22,-2.1 1,-0.1 -1,-0.2 -0.993 118.0 22.5-143.2 142.3 -13.9 5.5 30.8 44 48 L G S S- 0 0 43 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.1 0.642 94.0-173.0 79.2 16.5 -12.6 8.0 33.3 45 49 L G + 0 0 46 2,-0.1 -1,-0.3 -4,-0.1 2,-0.1 -0.549 56.8 54.0 -94.4 162.4 -14.5 10.9 31.8 46 50 L P S S- 0 0 95 0, 0.0 156,-0.2 0, 0.0 155,-0.2 0.544 98.5-117.5 -64.1 140.1 -14.9 13.8 31.8 47 51 L L - 0 0 6 154,-2.1 -2,-0.1 1,-0.2 4,-0.1 -0.283 17.4-150.4 -54.7 125.2 -11.2 13.9 31.3 48 52 L P S S+ 0 0 45 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.127 75.9 47.2 -86.8 20.3 -9.6 15.6 34.3 49 53 L F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 2,-0.2 -0.965 103.8 -70.6-151.1 164.9 -6.6 16.9 32.2 50 54 L A > - 0 0 2 76,-2.3 3,-1.4 -2,-0.3 4,-0.2 -0.378 33.3-146.0 -61.0 124.0 -6.1 18.7 28.9 51 55 L F G >> S+ 0 0 7 1,-0.3 3,-2.9 -2,-0.2 4,-0.6 0.781 88.9 84.5 -60.5 -26.5 -6.8 16.3 26.0 52 56 L D G >4 S+ 0 0 17 1,-0.3 3,-0.9 2,-0.2 4,-0.4 0.778 78.9 63.2 -46.8 -33.8 -4.0 18.1 24.1 53 57 L I G <4 S+ 0 0 2 -3,-1.4 -1,-0.3 1,-0.2 -2,-0.2 0.648 103.8 49.2 -69.7 -13.0 -1.4 15.8 25.9 54 58 L L G X> S+ 0 0 0 -3,-2.9 3,-1.9 -4,-0.2 4,-0.6 0.563 84.4 93.9 -97.8 -13.4 -3.0 12.8 24.1 55 59 L S G X< S+ 0 0 2 -3,-0.9 3,-1.1 -4,-0.6 -2,-0.1 0.838 82.4 50.3 -51.4 -47.6 -3.1 14.3 20.6 56 60 L T G 34 S+ 0 0 27 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.520 110.1 53.7 -73.8 -3.7 0.2 12.8 19.4 57 61 L S G <4 0 0 2 -3,-1.9 -1,-0.2 3,-0.0 -2,-0.2 0.554 360.0 360.0-102.1 -10.6 -0.9 9.4 20.5 58 62 L C << 0 0 6 -3,-1.1 -19,-0.0 -4,-0.6 -3,-0.0 -0.273 360.0 360.0 -75.2 360.0 -4.3 9.2 18.7 59 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 60 66 A S > 0 0 15 0, 0.0 3,-1.5 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 68.5 -1.1 8.0 12.7 61 67 A K T 3 + 0 0 12 1,-0.3 13,-0.2 13,-0.0 14,-0.2 0.410 360.0 79.1 -78.3 -1.2 2.0 7.9 10.7 62 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.1 12,-0.1 -1,-0.3 0.735 82.9 71.6 -74.2 -21.5 0.5 6.2 7.6 63 69 A F < + 0 0 0 -3,-1.5 2,-0.4 -30,-0.1 -1,-0.2 -0.655 68.1 123.7-101.8 79.0 -0.9 9.6 6.6 64 70 A I - 0 0 3 -2,-1.1 2,-0.8 -34,-1.0 -31,-0.3 -0.999 56.0-134.6-136.1 134.9 2.1 11.5 5.4 65 71 A K E -e 211 0A 89 145,-2.3 147,-2.7 -2,-0.4 2,-0.4 -0.818 26.2-156.8 -86.6 111.3 2.7 13.2 2.1 66 72 A Y E -e 212 0A 41 -2,-0.8 2,-0.2 -35,-0.2 3,-0.2 -0.781 13.7-177.4 -92.2 131.3 6.3 12.3 1.0 67 73 A V > + 0 0 56 145,-2.2 3,-1.0 -2,-0.4 144,-0.0 -0.761 54.2 34.0-119.3 169.2 8.1 14.6 -1.4 68 74 A S T 3 S- 0 0 73 -2,-0.2 -1,-0.1 1,-0.2 145,-0.1 0.690 123.2 -66.6 63.8 25.0 11.6 14.6 -3.2 69 75 A G T 3 S+ 0 0 76 1,-0.2 -1,-0.2 -3,-0.2 144,-0.1 0.817 82.4 161.6 67.2 33.3 11.7 10.7 -3.6 70 76 A I < - 0 0 10 -3,-1.0 -1,-0.2 142,-0.2 142,-0.1 -0.741 42.6-115.3 -83.7 127.0 12.0 10.0 0.2 71 77 A P - 0 0 31 0, 0.0 2,-0.9 0, 0.0 -1,-0.1 -0.360 21.6-126.6 -64.9 146.0 11.2 6.4 1.0 72 78 A D > - 0 0 26 1,-0.2 4,-2.3 111,-0.1 5,-0.2 -0.802 22.0-172.6-100.8 94.2 8.1 5.9 3.2 73 79 A Y H > S+ 0 0 5 -2,-0.9 4,-0.5 1,-0.2 -1,-0.2 0.816 83.4 47.0 -54.8 -35.2 9.1 3.8 6.2 74 80 A F H >4 S+ 0 0 0 -13,-0.2 3,-0.7 2,-0.2 4,-0.3 0.925 112.5 43.8 -81.0 -48.1 5.5 3.4 7.4 75 81 A K H >4 S+ 0 0 28 1,-0.2 3,-1.2 -14,-0.2 -2,-0.2 0.860 110.3 58.2 -67.2 -35.0 3.7 2.5 4.1 76 82 A Q H 3< S+ 0 0 52 -4,-2.3 -1,-0.2 1,-0.2 -2,-0.2 0.755 93.3 71.3 -57.9 -28.2 6.5 0.0 3.3 77 83 A S T - G 0 166A 14 4,-3.0 3,-1.8 -2,-0.5 76,-0.2 -0.686 18.9-125.8 -94.4 146.4 7.4 18.9 29.3 91 97 A E T 3 S+ 0 0 72 74,-2.5 75,-0.1 -2,-0.3 -1,-0.1 0.673 108.9 55.7 -65.7 -19.1 9.3 22.0 30.4 92 98 A D T 3 S- 0 0 40 73,-0.2 -1,-0.3 2,-0.1 32,-0.1 0.235 129.2 -88.6 -99.6 12.5 7.4 22.2 33.6 93 99 A G S < S+ 0 0 26 -3,-1.8 -2,-0.1 1,-0.3 27,-0.1 0.232 82.7 131.2 108.9 -12.0 8.2 18.6 34.7 94 100 A G - 0 0 0 -5,-0.1 -4,-3.0 27,-0.0 2,-0.4 -0.357 36.4-160.2 -71.1 150.7 5.4 16.5 33.2 95 101 A F E -FH 89 118A 11 23,-2.4 23,-2.4 -6,-0.2 2,-0.4 -0.996 6.9-169.0-129.9 139.6 6.2 13.3 31.2 96 102 A L E -FH 88 117A 3 -8,-2.5 -8,-2.7 -2,-0.4 2,-0.4 -0.940 10.5-174.8-130.7 111.9 3.9 11.5 28.7 97 103 A T E +FH 87 116A 0 19,-3.0 19,-2.8 -2,-0.4 2,-0.3 -0.850 6.0 175.3-109.9 138.3 4.9 8.1 27.6 98 104 A A E -FH 86 115A 2 -12,-2.6 -12,-2.4 -2,-0.4 2,-0.4 -0.983 21.0-163.8-141.7 148.9 3.2 6.0 24.9 99 105 A H E -FH 85 114A 8 15,-2.0 15,-2.6 -2,-0.3 2,-0.4 -0.996 19.1-169.9-128.3 135.1 3.5 2.8 23.0 100 106 A Q E -FH 84 113A 0 -16,-2.9 -16,-2.1 -2,-0.4 2,-0.5 -0.970 13.1-158.3-129.5 139.2 1.4 2.3 19.8 101 107 A D E -FH 83 112A 21 11,-2.5 11,-2.0 -2,-0.4 2,-0.5 -0.972 10.0-164.4-115.1 127.8 0.7 -0.7 17.6 102 108 A T E +FH 82 111A 0 -20,-3.0 -20,-1.4 -2,-0.5 2,-0.3 -0.964 14.9 168.7-114.6 125.2 -0.4 -0.2 14.0 103 109 A S E - 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0 0 4 -25,-0.2 -1,-0.2 1,-0.1 -27,-0.1 -0.591 49.9-108.5 -71.0 129.1 2.3 17.1 35.6 121 127 A P > - 0 0 19 0, 0.0 3,-1.6 0, 0.0 6,-0.6 -0.379 15.7-136.0 -63.3 134.9 2.1 20.1 37.9 122 128 A A T 3 S+ 0 0 43 1,-0.3 -2,-0.0 5,-0.1 -3,-0.0 0.794 107.4 46.6 -59.2 -27.4 -1.3 20.5 39.6 123 129 A D T 3 S+ 0 0 108 4,-0.1 -1,-0.3 5,-0.1 -3,-0.0 0.450 94.1 103.4 -94.0 -6.7 -1.2 24.2 38.9 124 130 A G S <> S- 0 0 5 -3,-1.6 4,-2.4 1,-0.1 5,-0.2 -0.302 86.9-103.5 -80.7 164.7 -0.1 23.9 35.2 125 131 A P T 4>S+ 0 0 26 0, 0.0 5,-2.7 0, 0.0 6,-0.5 0.738 116.8 58.0 -59.6 -25.4 -2.2 24.3 32.1 126 132 A V T >45S+ 0 0 1 3,-0.2 -76,-2.3 2,-0.2 3,-0.9 0.981 114.3 32.5 -68.5 -57.7 -2.4 20.5 31.6 127 133 A M T 345S+ 0 0 29 -6,-0.6 -1,-0.1 1,-0.3 -5,-0.1 0.797 119.5 53.3 -72.8 -28.1 -4.0 19.5 35.0 128 134 A Q T 3<5S- 0 0 94 -4,-2.4 -1,-0.3 -7,-0.2 -2,-0.2 0.514 107.8-130.7 -80.1 -6.1 -6.0 22.8 35.2 129 135 A N T < 5 + 0 0 46 -3,-0.9 -3,-0.2 -4,-0.2 -2,-0.1 0.906 68.4 127.7 56.1 47.6 -7.3 21.9 31.7 130 136 A K < + 0 0 117 -5,-2.7 2,-0.2 -6,-0.1 -4,-0.2 -0.074 27.7 132.4-122.9 31.6 -6.5 25.4 30.3 131 137 A A - 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