==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 03-MAR-08 3CFE . COMPND 2 MOLECULE: PURPLE-FLUORESCENT ANTIBODY EP2-25C10-KAPPA LIGHT . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR E.W.DEBLER,A.HEINE,I.A.WILSON . 864 4 8 8 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 40472.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 553 64.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 16 1.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 358 41.4 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 6 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 73 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 64 7.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 28 3.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 7 9 2 10 15 3 4 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 2 10 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 L D 0 0 130 0, 0.0 2,-0.4 0, 0.0 93,-0.0 0.000 360.0 360.0 360.0 124.9 12.9 -4.4 77.3 2 2 L I - 0 0 3 93,-0.4 2,-0.5 91,-0.1 88,-0.1 -0.595 360.0-137.7 -70.1 126.0 13.7 -0.8 76.6 3 3 L Q - 0 0 130 -2,-0.4 23,-2.2 94,-0.0 2,-0.4 -0.777 14.5-156.4 -87.9 129.2 10.4 0.9 76.0 4 4 L M E -A 25 0A 12 94,-0.5 2,-0.4 -2,-0.5 21,-0.2 -0.877 8.9-169.6-107.0 143.7 10.1 3.4 73.1 5 5 L T E -A 24 0A 36 19,-2.2 19,-1.9 -2,-0.4 2,-0.8 -0.982 19.3-172.2-138.4 118.9 7.6 6.2 73.0 6 6 L Q - 0 0 15 -2,-0.4 17,-0.1 17,-0.2 -2,-0.0 -0.880 16.0-161.2-108.5 98.5 6.6 8.5 70.2 7 7 L T S S+ 0 0 107 -2,-0.8 2,-0.5 15,-0.1 -1,-0.1 0.724 74.9 60.6 -59.6 -22.1 4.3 10.9 72.2 8 8 L T + 0 0 66 14,-0.3 14,-0.1 1,-0.1 3,-0.1 -0.933 51.4 169.3-107.5 131.5 2.7 12.0 68.9 9 9 L S + 0 0 73 -2,-0.5 94,-3.0 1,-0.2 2,-0.4 0.527 67.8 54.1-109.5 -14.9 0.9 9.6 66.7 10 10 L S E +d 103 0B 90 92,-0.2 2,-0.4 94,-0.0 94,-0.2 -0.985 59.2 170.1-126.4 136.7 -0.7 12.1 64.4 11 11 L L E -d 104 0B 46 92,-1.2 94,-3.4 -2,-0.4 2,-0.4 -0.996 7.8-174.4-148.3 133.0 1.0 14.8 62.4 12 12 L S E +d 105 0B 39 -2,-0.4 2,-0.3 92,-0.2 94,-0.2 -0.997 15.4 152.9-135.5 128.6 -0.3 17.0 59.6 13 13 L A E -d 106 0B 6 92,-1.9 94,-3.3 -2,-0.4 2,-0.1 -0.982 38.5-103.0-152.2 161.0 1.7 19.5 57.5 14 14 L S > - 0 0 47 -2,-0.3 3,-1.6 92,-0.2 64,-0.2 -0.357 50.0 -86.0 -80.3 159.6 1.8 21.3 54.2 15 15 L L T 3 S+ 0 0 86 1,-0.2 64,-0.2 92,-0.2 -1,-0.2 -0.348 117.0 28.3 -60.5 149.5 4.1 20.4 51.3 16 16 L G T 3 S+ 0 0 45 62,-2.0 61,-0.8 1,-0.2 -1,-0.2 0.157 96.7 126.2 82.9 -19.5 7.4 22.1 51.6 17 17 L D < - 0 0 70 -3,-1.6 61,-1.4 60,-0.2 2,-0.4 -0.088 60.0-123.5 -69.0 167.7 7.2 22.2 55.4 18 18 L R - 0 0 189 58,-0.2 2,-0.4 59,-0.2 58,-0.3 -0.892 31.0-175.3-106.4 142.0 9.6 20.9 58.0 19 19 L V E - B 0 75A 14 56,-3.0 56,-2.0 -2,-0.4 2,-0.4 -0.995 13.2-151.7-141.6 142.3 8.3 18.4 60.5 20 20 L T E - B 0 74A 75 -2,-0.4 2,-0.5 54,-0.2 54,-0.2 -0.962 3.9-167.4-115.9 134.0 9.8 16.7 63.5 21 21 L I E - B 0 73A 3 52,-2.4 52,-3.1 -2,-0.4 2,-0.3 -0.981 18.8-150.5-113.8 131.3 8.9 13.3 64.9 22 22 L S E - B 0 72A 51 -2,-0.5 2,-0.4 50,-0.2 -14,-0.3 -0.788 11.0-166.7-110.6 151.2 10.2 12.7 68.4 23 23 L a E - B 0 71A 2 48,-3.7 48,-3.0 -2,-0.3 2,-0.4 -0.999 2.1-166.5-140.0 134.9 11.2 9.4 70.0 24 24 L R E -AB 5 70A 136 -19,-1.9 -19,-2.2 -2,-0.4 2,-0.3 -0.981 7.2-148.8-130.8 138.0 12.0 8.6 73.7 25 25 L A E -A 4 0A 5 44,-2.5 -21,-0.2 -2,-0.4 4,-0.1 -0.793 21.6-130.5-109.8 141.2 13.6 5.6 75.4 26 26 L S S S+ 0 0 69 -23,-2.2 2,-0.3 -2,-0.3 -22,-0.1 0.761 95.0 9.6 -58.0 -24.8 13.0 4.2 78.8 27 27 L Q S S- 0 0 103 -24,-0.2 -1,-0.1 41,-0.1 -25,-0.0 -0.983 103.1 -64.0-149.9 163.7 16.8 4.2 79.4 28 28 L D + 0 0 94 -2,-0.3 41,-0.2 1,-0.1 -2,-0.1 -0.207 42.4 177.8 -48.9 125.6 20.0 5.6 77.8 29 29 L I > - 0 0 0 39,-1.8 3,-1.9 -4,-0.1 62,-0.2 0.153 34.5-130.6-121.5 11.3 20.5 3.9 74.4 30 30 L S T 3 - 0 0 56 1,-0.3 3,-0.1 38,-0.1 39,-0.1 0.785 65.5 -54.4 51.7 41.8 23.7 5.7 73.4 31 31 L N T 3 S+ 0 0 56 37,-0.4 2,-1.9 1,-0.2 -1,-0.3 0.326 95.7 131.7 77.2 -8.5 22.7 6.8 69.9 32 32 L Y < + 0 0 70 -3,-1.9 59,-1.9 18,-0.2 2,-0.4 -0.587 34.7 135.1 -72.2 78.1 21.8 3.4 68.6 33 33 L L E -E 90 0B 0 -2,-1.9 18,-2.0 57,-0.2 2,-0.3 -1.000 33.8-170.0-131.2 133.0 18.5 4.6 67.1 34 34 L N E -E 89 0B 0 55,-2.5 55,-3.2 -2,-0.4 2,-0.4 -0.822 10.5-145.8-118.1 155.6 17.1 3.8 63.7 35 35 L W E -EF 88 48B 0 13,-2.8 12,-3.8 -2,-0.3 13,-1.2 -0.997 13.8-169.3-128.4 131.1 14.2 5.2 61.9 36 36 L Y E -EF 87 46B 6 51,-3.7 51,-2.7 -2,-0.4 2,-0.5 -0.886 12.3-146.8-118.8 143.4 11.9 3.3 59.6 37 37 L Q E -EF 86 45B 13 8,-3.7 8,-2.6 -2,-0.3 2,-0.5 -0.956 11.2-166.4-107.6 125.1 9.2 4.5 57.1 38 38 L Q E -EF 85 44B 13 47,-2.8 47,-2.4 -2,-0.5 6,-0.2 -0.953 11.5-147.3-110.2 122.0 6.2 2.3 56.6 39 39 L K > - 0 0 43 4,-2.3 3,-3.5 -2,-0.5 45,-0.2 -0.391 31.9-103.5 -80.5 160.1 3.9 3.1 53.6 40 40 L P T 3 S+ 0 0 63 0, 0.0 -1,-0.1 0, 0.0 44,-0.1 0.886 124.3 60.3 -56.1 -35.0 0.2 2.5 53.7 41 41 L D T 3 S- 0 0 114 1,-0.1 -2,-0.0 2,-0.0 -3,-0.0 0.584 120.4-113.3 -67.0 -6.4 0.7 -0.6 51.6 42 42 L G < + 0 0 10 -3,-3.5 2,-0.2 1,-0.3 -1,-0.1 0.519 67.0 144.3 90.1 6.0 2.9 -1.9 54.5 43 43 L T - 0 0 40 -4,-0.0 -4,-2.3 284,-0.0 2,-0.4 -0.590 38.7-146.8 -74.3 145.0 6.3 -1.9 52.7 44 44 L V E -F 38 0B 18 281,-0.4 2,-0.4 -6,-0.2 280,-0.3 -0.953 17.7-177.7-123.4 136.5 9.0 -0.8 55.2 45 45 L K E -F 37 0B 135 -8,-2.6 -8,-3.7 -2,-0.4 2,-0.3 -0.995 26.0-125.1-137.8 138.1 12.1 1.1 54.6 46 46 L L E +F 36 0B 5 -2,-0.4 -10,-0.3 -10,-0.2 3,-0.1 -0.645 28.1 175.0 -79.5 135.4 15.0 2.2 56.8 47 47 L L E + 0 0 7 -12,-3.8 8,-2.0 1,-0.4 2,-0.4 0.841 61.0 3.9-104.0 -56.5 15.7 5.9 56.7 48 48 L I E -FG 35 54B 0 -13,-1.2 -13,-2.8 6,-0.2 -1,-0.4 -0.975 61.2-161.0-138.0 141.5 18.4 6.8 59.2 49 49 L Y E > + G 0 53B 38 4,-3.2 4,-1.7 -2,-0.4 -15,-0.2 -0.907 67.5 19.6-123.6 156.0 20.5 4.7 61.5 50 50 L Y T >4 S- 0 0 63 -2,-0.3 2,-2.8 268,-0.3 3,-0.6 0.952 124.4 -65.4 52.8 63.5 22.6 5.4 64.6 51 51 L T T 34 S+ 0 0 6 -18,-2.0 -1,-0.3 1,-0.2 21,-0.1 -0.326 130.4 15.9 61.9 -60.8 20.8 8.6 65.4 52 52 L S T 34 S+ 0 0 72 -2,-2.8 12,-0.4 -3,-0.2 2,-0.3 0.569 87.1 114.0-124.1 -11.9 21.8 10.6 62.3 53 53 L R E << -G 49 0B 106 -4,-1.7 -4,-3.2 -3,-0.6 2,-0.5 -0.502 57.7-131.7 -75.1 132.2 23.3 8.5 59.5 54 54 L L E -G 48 0B 62 -2,-0.3 -6,-0.2 -6,-0.2 3,-0.1 -0.645 17.6-125.7 -79.7 123.7 21.4 8.3 56.2 55 55 L H > - 0 0 40 -8,-2.0 3,-1.8 -2,-0.5 -1,-0.1 -0.426 46.4 -83.3 -60.7 137.2 20.8 4.8 54.7 56 56 L S T 3 S+ 0 0 111 1,-0.2 -1,-0.1 -2,-0.1 0, 0.0 -0.221 118.5 23.1 -39.0 122.3 22.1 4.6 51.1 57 57 L G T 3 S+ 0 0 79 1,-0.3 -1,-0.2 -3,-0.1 -10,-0.0 -0.183 89.2 125.1 108.8 -37.7 19.3 6.0 48.8 58 58 L V < - 0 0 22 -3,-1.8 -1,-0.3 -11,-0.2 4,-0.1 -0.357 67.2-109.8 -64.6 121.7 17.5 8.2 51.4 59 59 L P > - 0 0 57 0, 0.0 3,-1.9 0, 0.0 -1,-0.1 -0.218 19.8-125.5 -48.5 134.6 17.1 11.9 50.4 60 60 L S T 3 S+ 0 0 106 1,-0.3 -2,-0.1 -6,-0.1 -6,-0.0 0.723 103.9 81.9 -56.6 -23.6 19.2 14.3 52.5 61 61 L R T 3 S+ 0 0 57 16,-0.1 15,-2.1 14,-0.1 2,-0.4 0.510 81.7 82.5 -61.2 -3.2 16.0 16.2 53.4 62 62 L F E < +C 75 0A 7 -3,-1.9 2,-0.3 13,-0.2 13,-0.2 -0.919 62.2 157.0 -98.8 133.4 15.8 13.3 56.0 63 63 L S E -C 74 0A 51 11,-1.6 11,-3.8 -2,-0.4 2,-0.3 -0.998 23.6-154.2-156.2 152.5 17.9 13.9 59.2 64 64 L G E -C 73 0A 11 -12,-0.4 2,-0.3 -2,-0.3 9,-0.2 -0.947 8.7-179.2-134.5 155.5 18.1 12.8 62.8 65 65 L S E +C 72 0A 58 7,-2.4 7,-2.7 -2,-0.3 2,-0.2 -0.950 21.6 104.2-144.4 157.3 19.4 14.1 66.1 66 66 L G E -C 71 0A 30 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.621 30.0-150.4 149.4 161.3 19.6 13.0 69.7 67 67 L S E > -C 70 0A 83 3,-1.5 3,-1.9 -2,-0.2 2,-0.2 -0.958 63.6 -4.1-149.0 167.8 21.4 11.7 72.7 68 68 L G T 3 S- 0 0 25 -2,-0.3 -39,-1.8 1,-0.3 -37,-0.4 -0.299 128.5 -20.3 55.4-113.0 20.7 9.7 75.9 69 69 L T T 3 S+ 0 0 51 -2,-0.2 -44,-2.5 -41,-0.2 2,-0.4 0.603 122.3 79.0 -97.1 -17.5 17.1 8.9 76.0 70 70 L D E < +BC 24 67A 97 -3,-1.9 -3,-1.5 -46,-0.2 2,-0.3 -0.838 51.3 162.5-110.0 130.4 15.7 11.6 73.6 71 71 L Y E -BC 23 66A 9 -48,-3.0 -48,-3.7 -2,-0.4 2,-0.3 -0.966 15.1-161.5-142.9 155.6 15.8 11.5 69.9 72 72 L S E -BC 22 65A 24 -7,-2.7 -7,-2.4 -2,-0.3 2,-0.4 -0.998 10.6-148.9-141.1 137.8 14.2 13.2 66.9 73 73 L L E -BC 21 64A 0 -52,-3.1 -52,-2.4 -2,-0.3 2,-0.5 -0.823 17.5-158.9 -93.5 140.2 13.6 12.6 63.1 74 74 L T E -BC 20 63A 22 -11,-3.8 -11,-1.6 -2,-0.4 2,-0.5 -0.950 11.7-177.9-128.1 115.2 13.4 15.7 60.8 75 75 L I E +BC 19 62A 0 -56,-2.0 -56,-3.0 -2,-0.5 2,-0.2 -0.940 15.7 178.5-103.8 123.3 11.8 15.6 57.4 76 76 L S + 0 0 27 -15,-2.1 -58,-0.2 -2,-0.5 -59,-0.2 -0.686 58.9 34.5-120.1 176.2 12.0 19.0 55.7 77 77 L N S S- 0 0 112 -61,-0.8 -1,-0.2 -2,-0.2 -60,-0.2 0.940 95.4-140.5 36.9 65.6 10.9 20.1 52.2 78 78 L L - 0 0 3 -61,-1.4 -62,-2.0 -64,-0.2 2,-0.3 -0.116 19.5-150.3 -67.6 154.3 8.1 17.8 52.8 79 79 L D > - 0 0 59 -64,-0.2 3,-2.8 -63,-0.1 4,-0.2 -0.930 28.0-120.0-136.2 148.5 6.8 15.8 49.9 80 80 L Q G > S+ 0 0 51 -2,-0.3 3,-0.9 1,-0.3 26,-0.1 0.805 115.8 57.3 -51.7 -32.3 3.7 14.1 48.5 81 81 L D G 3 S+ 0 0 98 1,-0.2 -1,-0.3 88,-0.0 87,-0.0 0.472 86.9 79.6 -79.0 -3.0 5.5 10.8 48.7 82 82 L D G < + 0 0 2 -3,-2.8 2,-0.8 2,-0.1 -1,-0.2 0.485 54.1 123.3 -91.8 -1.6 6.1 11.2 52.4 83 83 L I < + 0 0 14 -3,-0.9 2,-0.3 -4,-0.2 22,-0.1 -0.469 47.8 102.6 -56.5 102.4 2.7 10.1 53.5 84 84 L A S S- 0 0 7 -2,-0.8 20,-2.0 20,-0.4 2,-0.4 -0.928 74.7 -92.5-163.7-176.2 3.6 7.2 55.8 85 85 L T E -EH 38 103B 17 -47,-2.4 -47,-2.8 -2,-0.3 2,-0.5 -0.964 37.5-142.4-113.7 138.8 4.0 6.2 59.4 86 86 L Y E -EH 37 102B 0 16,-2.3 16,-1.2 -2,-0.4 2,-0.4 -0.916 15.0-167.9-116.5 128.0 7.4 6.7 61.0 87 87 L F E -E 36 0B 3 -51,-2.7 -51,-3.7 -2,-0.5 2,-0.4 -0.921 9.4-151.7-121.3 129.4 8.9 4.3 63.4 88 88 L a E -E 35 0B 0 -2,-0.4 11,-1.8 -53,-0.2 2,-0.4 -0.829 14.4-162.0-100.4 139.7 11.9 4.7 65.7 89 89 L Q E -EI 34 98B 7 -55,-3.2 -55,-2.5 -2,-0.4 2,-0.4 -0.970 13.6-140.8-129.0 123.2 13.9 1.6 66.7 90 90 L Q E -EI 33 97B 0 7,-2.1 7,-1.0 -2,-0.4 -57,-0.2 -0.682 17.4-178.6 -79.7 127.3 16.4 1.1 69.6 91 91 L G + 0 0 14 -59,-1.9 -58,-0.1 -2,-0.4 -1,-0.1 -0.221 53.7 93.9-116.1 39.4 19.3 -1.1 68.5 92 92 L T S S+ 0 0 51 1,-0.3 2,-0.3 -60,-0.3 -1,-0.1 0.819 90.3 8.6-104.1 -37.0 21.4 -1.3 71.7 93 93 L T S S- 0 0 65 2,-0.2 -1,-0.3 -3,-0.1 4,-0.1 -0.906 91.6 -65.0-135.2 163.1 20.2 -4.5 73.5 94 94 L L S S+ 0 0 72 -2,-0.3 -92,-0.1 1,-0.1 3,-0.1 -0.646 106.7 46.4 -87.4 150.9 18.0 -7.4 72.6 95 95 L P S S- 0 0 4 0, 0.0 -93,-0.4 0, 0.0 -2,-0.2 0.703 86.8-136.8 -73.9 149.5 15.2 -7.6 72.0 96 96 L P - 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