==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 03-MAR-08 3CFF . COMPND 2 MOLECULE: GFP-LIKE PHOTOSWITCHABLE FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: ANEMONIA SULCATA; . AUTHOR G.S.KACHALOVA,S.GUNDEL,H.D.BARTUNIK,J.WIEDENMANN . 896 8 2 0 2 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32478.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 654 73.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 412 46.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 72 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 76 8.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 30 3.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 0 0 0 4 0 8 4 8 0 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 5 L L 0 0 77 0, 0.0 2,-0.2 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 160.4 -3.5 1.7 0.0 2 6 L T - 0 0 87 2,-0.1 29,-0.1 1,-0.1 0, 0.0 -0.562 360.0-112.4 -80.9 151.1 -6.8 3.3 -1.2 3 7 L E S S+ 0 0 134 -2,-0.2 2,-0.4 1,-0.2 29,-0.2 0.562 110.4 42.2 -67.1 -8.6 -6.9 7.1 -1.5 4 8 L T S S+ 0 0 69 25,-0.2 25,-0.2 26,-0.1 -1,-0.2 -0.948 76.4 179.3-134.6 120.9 -9.5 7.1 1.4 5 9 L M E -A 28 0A 7 23,-2.9 23,-2.0 -2,-0.4 2,-0.2 -0.969 16.2-145.5-125.2 128.1 -8.7 4.7 4.3 6 10 L P E -A 27 0A 39 0, 0.0 103,-2.2 0, 0.0 2,-0.3 -0.575 17.9-171.3 -87.8 157.7 -10.8 4.3 7.5 7 11 L F E -Ab 26 109A 1 19,-2.8 19,-2.6 101,-0.2 2,-0.3 -0.989 12.0-156.6-143.8 154.9 -9.3 3.5 10.9 8 12 L R E -Ab 25 110A 85 101,-2.4 103,-3.0 -2,-0.3 2,-0.3 -0.978 12.7-163.3-131.9 149.6 -10.4 2.5 14.4 9 13 L M E -Ab 24 111A 4 15,-2.5 15,-2.3 -2,-0.3 2,-0.4 -0.960 13.2-170.3-136.2 141.3 -8.6 3.0 17.7 10 14 L T E -Ab 23 112A 78 101,-1.9 103,-2.8 -2,-0.3 2,-0.4 -0.994 15.8-175.0-126.5 141.2 -8.7 1.7 21.3 11 15 L M E -Ab 22 113A 6 11,-2.8 11,-1.9 -2,-0.4 2,-0.4 -0.987 10.1-175.7-134.6 140.7 -6.7 3.4 24.1 12 16 L E E +Ab 21 114A 97 101,-2.1 103,-2.2 -2,-0.4 2,-0.3 -0.982 22.0 146.0-128.6 148.3 -6.1 2.6 27.7 13 17 L G E -Ab 20 115A 6 7,-2.1 7,-2.6 -2,-0.4 2,-0.4 -0.995 39.3-138.9-168.0 167.7 -4.2 4.7 30.2 14 18 L T E -Ab 19 116A 6 101,-1.5 103,-3.0 -2,-0.3 2,-0.4 -0.993 20.7-178.0-132.4 128.2 -3.5 6.1 33.6 15 19 L V E > S-Ab 18 117A 0 3,-2.2 3,-2.1 -2,-0.4 103,-0.2 -0.981 74.5 -19.4-128.7 119.5 -2.5 9.8 34.0 16 20 L N T 3 S- 0 0 19 101,-2.3 102,-0.1 -2,-0.4 -1,-0.1 0.867 131.2 -48.1 52.7 39.9 -1.6 11.1 37.4 17 21 L G T 3 S+ 0 0 26 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.235 111.8 122.4 88.3 -13.1 -3.5 8.2 39.0 18 22 L H E < -A 15 0A 62 -3,-2.1 -3,-2.2 30,-0.0 2,-0.3 -0.756 50.2-151.3 -90.5 117.3 -6.6 8.6 36.8 19 23 L H E +A 14 0A 95 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.615 25.4 163.1 -83.5 142.2 -7.5 5.5 34.8 20 24 L F E -A 13 0A 4 -7,-2.6 -7,-2.1 -2,-0.3 2,-0.3 -0.979 25.5-152.8-154.2 162.8 -9.3 6.0 31.5 21 25 L K E -AC 12 43A 58 22,-2.3 21,-1.7 -2,-0.3 22,-1.6 -0.996 12.6-172.9-140.9 141.0 -10.3 4.3 28.2 22 26 L C E -AC 11 41A 2 -11,-1.9 -11,-2.8 -2,-0.3 2,-0.3 -0.955 9.0-160.1-131.1 150.7 -11.0 5.8 24.8 23 27 L T E -AC 10 40A 48 17,-2.3 17,-2.8 -2,-0.3 2,-0.3 -0.907 15.2-166.7-120.7 158.1 -12.3 4.6 21.5 24 28 L G E -AC 9 39A 7 -15,-2.3 -15,-2.5 -2,-0.3 2,-0.3 -0.994 13.3-171.7-147.9 150.5 -11.8 6.3 18.2 25 29 L K E +AC 8 38A 113 13,-2.3 13,-3.1 -2,-0.3 2,-0.3 -0.988 24.8 135.8-143.6 131.5 -13.1 6.2 14.6 26 30 L G E -AC 7 37A 17 -19,-2.6 -19,-2.8 -2,-0.3 2,-0.3 -0.919 30.9-144.4-156.8-178.5 -11.8 8.1 11.6 27 31 L E E +AC 6 36A 112 9,-2.1 9,-2.5 -21,-0.3 2,-0.3 -0.976 20.1 167.6-150.9 158.7 -10.8 8.2 8.0 28 32 L G E -AC 5 35A 5 -23,-2.0 -23,-2.9 -2,-0.3 7,-0.3 -0.979 42.8 -97.5-160.0 172.8 -8.2 10.0 5.9 29 33 L N E >> - C 0 34A 40 5,-1.9 5,-1.6 -2,-0.3 4,-1.1 -0.894 33.5-166.3 -97.4 103.0 -6.3 10.3 2.6 30 34 L P T 45S+ 0 0 0 0, 0.0 34,-0.9 0, 0.0 -1,-0.1 0.815 85.8 43.0 -62.9 -36.0 -3.0 8.4 3.2 31 35 L F T 45S+ 0 0 100 1,-0.2 35,-0.2 32,-0.1 -28,-0.1 0.697 117.0 47.0 -84.5 -20.0 -1.1 9.7 0.2 32 36 L E T 45S- 0 0 100 -3,-0.3 33,-0.3 -29,-0.2 -1,-0.2 0.514 107.5-128.7 -94.7 -10.1 -2.3 13.3 0.7 33 37 L G T <5 + 0 0 1 -4,-1.1 177,-2.5 31,-0.3 2,-0.3 0.773 62.8 125.4 70.5 31.4 -1.5 13.1 4.5 34 38 L T E < +CD 29 209A 40 -5,-1.6 -5,-1.9 175,-0.2 2,-0.3 -0.915 28.4 166.1-122.8 149.6 -4.8 14.3 5.9 35 39 L Q E -CD 28 208A 10 173,-1.9 173,-2.4 -2,-0.3 2,-0.3 -0.991 17.8-162.4-160.1 152.7 -7.1 12.7 8.4 36 40 L D E +CD 27 207A 66 -9,-2.5 -9,-2.1 -2,-0.3 2,-0.3 -0.981 17.2 173.4-135.7 144.8 -10.1 13.3 10.7 37 41 L M E -CD 26 206A 12 169,-2.2 169,-2.7 -2,-0.3 2,-0.4 -0.995 26.7-134.0-150.7 157.6 -11.3 11.3 13.6 38 42 L K E -CD 25 205A 140 -13,-3.1 -13,-2.3 -2,-0.3 2,-0.4 -0.919 21.9-162.8-108.2 138.2 -13.8 11.2 16.5 39 43 L I E -CD 24 204A 2 165,-2.7 165,-2.9 -2,-0.4 2,-0.4 -0.986 3.5-168.6-123.4 131.4 -12.7 10.2 20.0 40 44 L E E -CD 23 203A 81 -17,-2.8 -17,-2.3 -2,-0.4 2,-0.8 -0.983 20.5-137.4-121.8 131.2 -14.9 9.1 22.8 41 45 L V E +C 22 0A 5 161,-2.9 -19,-0.3 -2,-0.4 4,-0.1 -0.758 27.8 172.6 -80.8 107.8 -14.0 8.7 26.5 42 46 L I E + 0 0 78 -21,-1.7 2,-0.3 -2,-0.8 -20,-0.2 0.574 65.4 27.2 -97.6 -13.2 -15.7 5.4 27.4 43 47 L E E S+C 21 0A 99 -22,-1.6 -22,-2.3 1,-0.0 -1,-0.2 -0.979 118.6 22.1-148.7 139.2 -14.2 5.0 30.9 44 48 L G S S- 0 0 30 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.0 0.643 94.0-171.2 80.5 16.9 -12.9 7.6 33.4 45 49 L G + 0 0 32 2,-0.1 -1,-0.3 -4,-0.1 157,-0.1 -0.549 57.4 49.7 -91.8 159.2 -14.9 10.4 31.9 46 50 L P S S- 0 0 78 0, 0.0 156,-0.2 0, 0.0 155,-0.2 0.549 96.8-121.8 -69.6 138.4 -15.2 13.3 32.0 47 51 L L - 0 0 6 154,-2.1 -2,-0.1 1,-0.2 4,-0.1 -0.269 13.4-149.7 -53.4 126.8 -11.4 13.4 31.6 48 52 L P S S+ 0 0 52 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.123 76.5 51.4 -86.3 21.2 -9.9 15.3 34.6 49 53 L F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 77,-0.1 -0.978 102.9 -71.6-151.1 161.5 -7.0 16.5 32.4 50 54 L A > - 0 0 1 76,-2.5 3,-1.3 -2,-0.3 4,-0.2 -0.311 32.2-146.3 -57.6 124.4 -6.4 18.3 29.1 51 55 L F G >> S+ 0 0 6 1,-0.2 3,-3.1 2,-0.2 4,-0.7 0.790 89.2 83.9 -60.6 -27.8 -7.2 15.9 26.2 52 56 L D G >4 S+ 0 0 18 1,-0.3 3,-0.8 2,-0.2 4,-0.5 0.789 78.9 64.0 -46.8 -34.9 -4.3 17.7 24.3 53 57 L I G <4 S+ 0 0 1 -3,-1.3 -1,-0.3 1,-0.2 -2,-0.2 0.651 104.7 46.9 -67.3 -13.6 -1.8 15.4 26.0 54 58 L L G X> S+ 0 0 0 -3,-3.1 3,-2.3 -4,-0.2 4,-0.7 0.593 85.6 92.8 -99.0 -14.3 -3.4 12.4 24.2 55 59 L S G X< S+ 0 0 1 -3,-0.8 3,-1.0 -4,-0.7 -2,-0.1 0.832 82.5 52.4 -51.7 -44.5 -3.6 14.0 20.7 56 60 L T G 34 S+ 0 0 25 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.501 111.9 48.9 -74.8 -1.2 -0.2 12.6 19.5 57 61 L S G <4 0 0 2 -3,-2.3 -1,-0.2 3,-0.0 -2,-0.2 0.507 360.0 360.0-107.8 -9.6 -1.3 9.1 20.5 58 62 L C << 0 0 8 -3,-1.0 -19,-0.0 -4,-0.7 -47,-0.0 -0.351 360.0 360.0 -76.5 360.0 -4.8 9.0 18.9 59 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 60 66 A S > 0 0 15 0, 0.0 3,-1.4 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 68.8 -1.4 7.9 12.9 61 67 A K T 3 + 0 0 9 1,-0.3 13,-0.2 13,-0.0 14,-0.2 0.477 360.0 78.1 -77.5 -6.9 1.7 7.7 10.7 62 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.2 12,-0.1 -1,-0.3 0.760 83.8 72.0 -68.9 -23.8 0.1 6.0 7.7 63 69 A F < + 0 0 0 -3,-1.4 2,-0.4 -30,-0.1 -1,-0.2 -0.629 69.5 122.7 -99.4 76.3 -1.4 9.4 6.8 64 70 A I - 0 0 2 -2,-1.2 2,-0.7 -34,-0.9 -31,-0.3 -0.999 55.7-136.0-137.3 133.5 1.6 11.3 5.5 65 71 A K E -e 211 0A 88 145,-2.1 147,-2.8 -2,-0.4 2,-0.4 -0.808 25.7-155.9 -87.0 114.0 2.3 13.0 2.2 66 72 A Y E -e 212 0A 40 -2,-0.7 2,-0.3 -35,-0.2 3,-0.2 -0.789 13.8-178.4 -94.9 132.1 5.8 12.2 1.1 67 73 A V > + 0 0 57 145,-2.1 3,-0.9 -2,-0.4 144,-0.0 -0.802 54.9 34.9-121.7 166.9 7.7 14.5 -1.3 68 74 A S T 3 S- 0 0 70 -2,-0.3 -1,-0.1 1,-0.2 145,-0.1 0.742 123.0 -67.2 65.2 28.8 11.2 14.5 -3.0 69 75 A G T 3 S+ 0 0 77 1,-0.2 -1,-0.2 -3,-0.2 144,-0.1 0.803 82.7 161.4 63.0 31.0 11.4 10.7 -3.4 70 76 A I < - 0 0 10 -3,-0.9 -1,-0.2 142,-0.2 142,-0.1 -0.741 42.3-117.2 -80.9 125.4 11.6 10.0 0.3 71 77 A P - 0 0 31 0, 0.0 2,-0.9 0, 0.0 -4,-0.1 -0.377 20.4-126.0 -65.0 145.6 10.7 6.4 1.1 72 78 A D > - 0 0 25 1,-0.2 4,-2.3 -2,-0.1 5,-0.2 -0.796 22.4-172.8-102.5 93.9 7.6 5.9 3.2 73 79 A Y T 4 S+ 0 0 5 -2,-0.9 4,-0.5 1,-0.2 -1,-0.2 0.804 83.4 47.7 -56.0 -34.1 8.7 3.7 6.2 74 80 A F T >4 S+ 0 0 0 -13,-0.2 3,-0.7 2,-0.2 4,-0.3 0.939 112.7 43.0 -80.5 -49.2 5.1 3.3 7.4 75 81 A K G >4 S+ 0 0 28 1,-0.2 3,-1.3 -14,-0.2 -2,-0.2 0.859 110.6 58.6 -65.7 -34.1 3.3 2.4 4.2 76 82 A Q G 3< S+ 0 0 49 -4,-2.3 -1,-0.2 1,-0.2 -2,-0.2 0.760 93.1 70.9 -59.5 -26.5 6.1 0.0 3.3 77 83 A S G <> S+ 0 0 0 -3,-0.7 4,-2.1 -4,-0.5 3,-0.3 0.598 81.9 137.4 -73.8 -12.9 5.4 -1.8 6.6 78 84 A F T <4 + 0 0 25 -3,-1.3 25,-0.0 1,-0.3 96,-0.0 -0.546 54.5 25.0 -80.7 148.4 2.1 -3.3 5.3 79 85 A P T 4 S+ 0 0 96 0, 0.0 25,-0.6 0, 0.0 -1,-0.3 -0.987 131.0 44.2 -80.6 -14.8 0.7 -5.9 5.5 80 86 A E T 4 S- 0 0 78 -3,-0.3 -2,-0.3 1,-0.2 2,-0.1 0.812 96.8-156.0 -59.6 -35.7 2.7 -6.6 8.7 81 87 A G < - 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