==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 03-MAR-08 3CFH . COMPND 2 MOLECULE: GFP-LIKE PHOTOSWITCHABLE FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: ANEMONIA SULCATA; . AUTHOR G.S.KACHALOVA,S.GUNDEL,H.D.BARTUNIK,J.WIEDENMANN . 896 8 2 0 2 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32487.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 656 73.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 412 46.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 73 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 76 8.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 32 3.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 0 0 0 4 0 8 4 8 0 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 5 L L 0 0 77 0, 0.0 2,-0.2 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 158.3 -3.6 1.7 0.0 2 6 L T - 0 0 84 2,-0.1 29,-0.1 1,-0.1 0, 0.0 -0.610 360.0-114.2 -81.5 147.5 -6.9 3.2 -1.2 3 7 L E S S+ 0 0 137 -2,-0.2 2,-0.4 26,-0.1 29,-0.2 0.632 109.7 41.0 -65.9 -11.7 -6.9 7.0 -1.6 4 8 L T S S+ 0 0 74 25,-0.2 25,-0.2 26,-0.1 -2,-0.1 -0.995 77.1 179.1-128.6 133.5 -9.4 7.1 1.3 5 9 L M E -A 28 0A 5 23,-2.8 23,-2.0 -2,-0.4 2,-0.2 -0.996 16.7-144.4-138.2 130.3 -9.0 4.8 4.3 6 10 L P E -A 27 0A 42 0, 0.0 103,-2.2 0, 0.0 2,-0.3 -0.583 18.3-170.3 -88.9 157.9 -11.0 4.3 7.5 7 11 L F E -Ab 26 109A 0 19,-2.8 19,-2.8 101,-0.2 2,-0.3 -0.985 11.5-156.4-143.1 157.3 -9.5 3.5 10.9 8 12 L R E -Ab 25 110A 96 101,-2.5 103,-3.3 -2,-0.3 2,-0.3 -0.968 12.0-163.2-132.5 152.4 -10.6 2.4 14.4 9 13 L M E -Ab 24 111A 5 15,-2.4 15,-2.1 -2,-0.3 2,-0.4 -0.973 13.9-169.7-137.8 142.8 -8.9 2.9 17.8 10 14 L T E -Ab 23 112A 78 101,-2.0 103,-2.6 -2,-0.3 2,-0.4 -0.994 17.1-174.5-127.9 138.9 -9.0 1.5 21.3 11 15 L M E -Ab 22 113A 1 11,-2.7 11,-2.5 -2,-0.4 2,-0.4 -0.998 10.4-176.3-137.3 134.9 -7.0 3.2 24.1 12 16 L E E +Ab 21 114A 115 101,-2.2 103,-2.4 -2,-0.4 2,-0.3 -0.996 22.5 149.5-129.8 139.2 -6.4 2.4 27.7 13 17 L G E -Ab 20 115A 10 7,-2.4 7,-2.4 -2,-0.4 2,-0.4 -0.980 38.9-141.7-160.2 166.4 -4.4 4.6 30.1 14 18 L T E -Ab 19 116A 6 101,-1.5 103,-3.1 -2,-0.3 2,-0.4 -0.981 19.6-177.6-134.0 127.1 -3.6 6.0 33.5 15 19 L V E > S-Ab 18 117A 0 3,-2.3 3,-2.1 -2,-0.4 103,-0.2 -0.977 74.9 -21.4-126.9 118.5 -2.7 9.7 33.9 16 20 L N T 3 S- 0 0 20 101,-2.3 102,-0.1 -2,-0.4 3,-0.1 0.843 131.0 -47.2 52.2 36.9 -1.8 11.0 37.4 17 21 L G T 3 S+ 0 0 28 100,-0.4 2,-0.6 1,-0.2 -1,-0.3 0.244 112.0 122.0 93.0 -13.2 -3.7 8.0 39.0 18 22 L H E < -A 15 0A 60 -3,-2.1 -3,-2.3 30,-0.0 2,-0.3 -0.759 49.8-152.1 -90.9 119.9 -6.8 8.4 36.8 19 23 L H E +A 14 0A 88 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.657 24.3 161.5 -88.5 142.5 -7.7 5.4 34.8 20 24 L F E -A 13 0A 5 -7,-2.4 -7,-2.4 -2,-0.3 2,-0.3 -0.978 25.9-151.4-154.1 162.1 -9.6 5.8 31.5 21 25 L K E -AC 12 43A 60 22,-2.1 21,-1.7 -2,-0.3 22,-1.7 -0.994 14.4-172.1-137.6 142.1 -10.5 4.1 28.2 22 26 L C E -AC 11 41A 1 -11,-2.5 -11,-2.7 -2,-0.3 2,-0.3 -0.953 9.0-160.0-130.0 151.2 -11.2 5.6 24.9 23 27 L T E -AC 10 40A 46 17,-2.4 17,-2.7 -2,-0.3 2,-0.3 -0.906 14.2-169.6-120.8 160.3 -12.5 4.4 21.5 24 28 L G E -AC 9 39A 9 -15,-2.1 -15,-2.4 -2,-0.3 2,-0.3 -0.993 13.8-170.9-148.5 151.8 -12.1 6.2 18.2 25 29 L K E +AC 8 38A 118 13,-2.0 13,-2.9 -2,-0.3 2,-0.3 -0.983 25.4 143.1-144.3 131.6 -13.3 6.0 14.6 26 30 L G E -AC 7 37A 12 -19,-2.8 -19,-2.8 -2,-0.3 2,-0.3 -0.903 28.9-147.4-152.3-179.6 -11.9 8.0 11.7 27 31 L E E +AC 6 36A 115 9,-2.3 9,-2.5 -21,-0.3 2,-0.3 -0.979 19.5 162.9-152.9 157.4 -11.0 8.2 8.1 28 32 L G E -AC 5 35A 7 -23,-2.0 -23,-2.8 -2,-0.3 7,-0.3 -0.954 44.5 -93.1-160.0 178.7 -8.4 10.0 6.0 29 33 L N E >>> - C 0 34A 38 5,-2.5 4,-1.4 -2,-0.3 5,-1.2 -0.888 33.9-163.9-104.4 100.6 -6.6 10.3 2.7 30 34 L P T 345S+ 0 0 0 0, 0.0 34,-1.1 0, 0.0 -1,-0.2 0.844 86.9 39.8 -58.3 -40.3 -3.4 8.3 3.2 31 35 L F T 345S+ 0 0 87 1,-0.2 35,-0.2 -28,-0.2 -28,-0.1 0.692 118.1 48.0 -84.0 -19.0 -1.4 9.7 0.2 32 36 L E T <45S- 0 0 104 -3,-0.6 33,-0.4 -29,-0.2 -1,-0.2 0.512 106.0-129.9 -94.0 -11.6 -2.6 13.3 0.8 33 37 L G T <5 + 0 0 1 -4,-1.4 177,-2.5 31,-0.4 2,-0.3 0.807 61.7 125.7 67.7 36.2 -1.8 13.1 4.5 34 38 L T E < +CD 29 209A 42 -5,-1.2 -5,-2.5 175,-0.2 2,-0.3 -0.939 28.3 166.0-124.0 147.0 -5.1 14.3 5.9 35 39 L Q E -CD 28 208A 11 173,-1.8 173,-2.3 -2,-0.3 2,-0.3 -0.991 17.5-164.4-158.0 153.0 -7.4 12.7 8.5 36 40 L D E +CD 27 207A 59 -9,-2.5 -9,-2.3 -2,-0.3 2,-0.3 -0.980 16.1 171.9-136.8 147.2 -10.4 13.2 10.8 37 41 L M E -CD 26 206A 12 169,-2.0 169,-2.4 -2,-0.3 2,-0.4 -0.990 27.8-131.0-150.9 160.1 -11.7 11.2 13.7 38 42 L K E -CD 25 205A 131 -13,-2.9 -13,-2.0 -2,-0.3 2,-0.4 -0.909 22.1-162.6-106.4 141.8 -14.1 11.1 16.6 39 43 L I E -CD 24 204A 0 165,-2.8 165,-2.9 -2,-0.4 2,-0.4 -0.996 3.5-168.7-125.5 130.9 -12.9 10.1 20.1 40 44 L E E -CD 23 203A 81 -17,-2.7 -17,-2.4 -2,-0.4 2,-0.8 -0.980 20.6-137.3-120.5 131.0 -15.2 8.9 22.9 41 45 L V E +C 22 0A 6 161,-2.8 -19,-0.3 -2,-0.4 4,-0.1 -0.781 27.6 173.7 -82.0 109.9 -14.2 8.5 26.5 42 46 L I E + 0 0 81 -21,-1.7 2,-0.3 -2,-0.8 -20,-0.2 0.635 65.3 25.6 -96.8 -19.1 -15.9 5.2 27.4 43 47 L E E S+C 21 0A 98 -22,-1.7 -22,-2.1 1,-0.1 -1,-0.2 -0.991 118.5 23.2-143.7 142.2 -14.5 4.8 30.9 44 48 L G S S- 0 0 29 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.1 0.626 93.6-173.4 80.9 15.4 -13.1 7.3 33.4 45 49 L G + 0 0 32 2,-0.1 -1,-0.3 -4,-0.1 2,-0.1 -0.567 56.3 54.0 -95.5 161.9 -15.0 10.3 32.0 46 50 L P S S- 0 0 80 0, 0.0 156,-0.2 0, 0.0 155,-0.2 0.511 97.5-117.7 -65.8 140.7 -15.5 13.1 32.1 47 51 L L - 0 0 5 154,-1.9 -2,-0.1 1,-0.2 4,-0.1 -0.300 17.1-149.8 -52.9 123.2 -11.7 13.3 31.5 48 52 L P S S+ 0 0 52 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.022 76.0 46.1 -87.0 26.5 -10.1 15.0 34.6 49 53 L F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 2,-0.1 -0.969 103.9 -68.5-158.2 163.8 -7.3 16.4 32.5 50 54 L A > - 0 0 2 76,-2.5 3,-1.4 -2,-0.3 4,-0.2 -0.340 33.9-144.6 -60.6 124.6 -6.7 18.2 29.1 51 55 L F G > S+ 0 0 6 1,-0.3 3,-2.9 2,-0.2 4,-0.5 0.789 89.6 83.3 -59.1 -27.0 -7.5 15.9 26.2 52 56 L D G > S+ 0 0 15 1,-0.3 3,-1.0 2,-0.2 4,-0.4 0.750 78.9 64.6 -51.4 -30.1 -4.6 17.6 24.3 53 57 L I G < S+ 0 0 2 -3,-1.4 -1,-0.3 1,-0.2 -2,-0.2 0.674 104.4 47.2 -68.4 -16.0 -2.0 15.3 26.1 54 58 L L G X> S+ 0 0 0 -3,-2.9 3,-1.8 -4,-0.2 4,-0.6 0.485 85.5 94.7 -98.6 -7.1 -3.7 12.3 24.3 55 59 L S G X4 S+ 0 0 3 -3,-1.0 3,-1.0 -4,-0.5 -2,-0.1 0.874 81.6 51.0 -57.5 -45.1 -3.7 14.0 20.8 56 60 L T G 34 S+ 0 0 29 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.446 111.3 51.0 -75.2 1.6 -0.5 12.5 19.5 57 61 L S G <4 0 0 2 -3,-1.8 -1,-0.2 3,-0.0 -2,-0.2 0.526 360.0 360.0-111.5 -7.4 -1.6 9.0 20.5 58 62 L C << 0 0 7 -3,-1.0 -19,-0.0 -4,-0.6 -3,-0.0 -0.287 360.0 360.0 -67.2 360.0 -5.1 9.0 18.8 59 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 60 66 A S > 0 0 19 0, 0.0 3,-1.7 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 73.3 -1.9 7.8 12.7 61 67 A K T 3 + 0 0 9 1,-0.3 13,-0.2 13,-0.0 14,-0.2 0.524 360.0 76.3 -73.1 -10.2 1.4 7.7 10.7 62 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.3 12,-0.1 -1,-0.3 0.793 84.0 72.9 -65.4 -27.7 -0.2 6.0 7.7 63 69 A F < + 0 0 1 -3,-1.7 2,-0.3 -30,-0.1 -1,-0.2 -0.630 69.1 119.8 -95.9 79.0 -1.7 9.4 6.8 64 70 A I - 0 0 1 -2,-1.3 2,-0.7 -34,-1.1 -31,-0.4 -0.997 57.0-134.5-140.2 137.0 1.3 11.4 5.5 65 71 A K E -e 211 0A 86 145,-2.0 147,-3.0 -33,-0.4 2,-0.5 -0.842 24.6-155.4 -90.7 116.3 2.0 13.0 2.2 66 72 A Y E +e 212 0A 43 -2,-0.7 2,-0.2 -35,-0.2 3,-0.2 -0.834 14.9 179.9 -96.9 125.6 5.6 12.2 1.1 67 73 A V > + 0 0 57 145,-2.4 3,-1.1 -2,-0.5 144,-0.0 -0.692 54.6 36.3-114.1 171.2 7.3 14.5 -1.4 68 74 A S T 3 S- 0 0 69 1,-0.2 -1,-0.1 -2,-0.2 145,-0.1 0.712 123.3 -67.8 62.9 27.2 10.8 14.5 -3.0 69 75 A G T 3 S+ 0 0 78 1,-0.2 -1,-0.2 -3,-0.2 -2,-0.1 0.780 82.9 160.9 64.6 28.8 11.0 10.7 -3.5 70 76 A I < - 0 0 9 -3,-1.1 -1,-0.2 142,-0.2 142,-0.1 -0.725 42.4-117.8 -78.7 124.4 11.3 10.1 0.3 71 77 A P - 0 0 31 0, 0.0 2,-0.9 0, 0.0 -4,-0.1 -0.368 19.9-125.5 -67.6 146.5 10.4 6.4 1.0 72 78 A D > - 0 0 25 1,-0.2 4,-2.2 111,-0.1 5,-0.2 -0.791 22.6-173.4-101.7 96.1 7.4 5.8 3.2 73 79 A Y H > S+ 0 0 3 -2,-0.9 4,-0.6 1,-0.2 -1,-0.2 0.824 83.2 47.9 -58.3 -34.2 8.4 3.7 6.2 74 80 A F H >4 S+ 0 0 0 -13,-0.2 3,-0.8 2,-0.2 4,-0.3 0.943 112.4 43.1 -79.7 -50.4 4.8 3.3 7.4 75 81 A K H >4 S+ 0 0 26 1,-0.2 3,-1.5 -14,-0.2 -2,-0.2 0.879 109.8 59.8 -64.3 -36.3 3.0 2.4 4.2 76 82 A Q H 3< S+ 0 0 53 -4,-2.2 -1,-0.2 1,-0.2 -2,-0.2 0.777 92.2 70.4 -56.3 -28.8 5.8 -0.0 3.3 77 83 A S T - G 0 166A 13 4,-2.9 3,-1.9 -2,-0.6 76,-0.2 -0.631 18.9-126.1 -91.2 146.4 6.8 18.3 29.4 91 97 A E T 3 S+ 0 0 73 74,-2.5 75,-0.1 1,-0.3 -1,-0.1 0.703 109.4 55.6 -66.8 -18.6 8.7 21.4 30.6 92 98 A D T 3 S- 0 0 38 73,-0.2 -1,-0.3 678,-0.0 32,-0.1 0.303 129.0 -90.3 -96.5 8.9 6.7 21.6 33.9 93 99 A G S < S+ 0 0 27 -3,-1.9 -2,-0.1 1,-0.3 2,-0.1 0.316 81.8 132.3 107.7 -6.8 7.6 18.0 34.8 94 100 A G - 0 0 1 -5,-0.0 -4,-2.9 27,-0.0 2,-0.4 -0.384 36.0-160.4 -72.8 153.6 4.8 15.9 33.3 95 101 A F E -FH 89 118A 11 23,-2.4 23,-2.6 -6,-0.2 2,-0.4 -0.999 6.8-169.2-133.7 138.2 5.6 12.8 31.3 96 102 A L E -FH 88 117A 2 -8,-2.3 -8,-2.9 -2,-0.4 2,-0.4 -0.963 9.6-174.1-129.7 115.9 3.3 11.0 28.9 97 103 A T E +FH 87 116A 0 19,-2.9 19,-2.9 -2,-0.4 2,-0.3 -0.895 6.0 175.9-113.0 136.9 4.4 7.6 27.6 98 104 A A E -FH 86 115A 3 -12,-2.4 -12,-2.3 -2,-0.4 2,-0.4 -0.983 19.9-164.9-138.0 148.1 2.6 5.6 24.9 99 105 A H E -FH 85 114A 9 15,-2.0 15,-2.6 -2,-0.3 2,-0.4 -0.996 19.5-170.9-126.2 137.8 2.9 2.3 23.0 100 106 A Q E -FH 84 113A 1 -16,-2.9 -16,-2.0 -2,-0.4 2,-0.5 -0.973 14.7-157.0-132.2 142.1 0.8 1.8 19.9 101 107 A D E -FH 83 112A 13 11,-2.5 11,-2.2 -2,-0.4 2,-0.5 -0.980 9.6-165.4-116.6 129.6 0.1 -1.1 17.6 102 108 A T E +FH 82 111A 1 -20,-2.9 -20,-1.3 -2,-0.5 2,-0.3 -0.973 15.6 167.7-117.5 121.6 -1.1 -0.4 14.0 103 109 A S E - 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0 0 6 -25,-0.2 -1,-0.2 1,-0.1 -27,-0.1 -0.548 50.2-107.1 -68.3 130.3 1.7 16.5 35.8 121 127 A P > - 0 0 24 0, 0.0 3,-1.1 0, 0.0 6,-0.6 -0.382 16.8-136.3 -63.3 136.4 1.6 19.5 38.1 122 128 A A T 3 S+ 0 0 50 1,-0.3 -2,-0.1 5,-0.1 475,-0.0 0.802 107.5 45.5 -60.1 -28.5 -1.8 19.8 39.9 123 129 A D T 3 S+ 0 0 107 4,-0.1 -1,-0.3 5,-0.1 -3,-0.0 0.504 95.3 104.5 -92.2 -10.5 -1.8 23.5 39.2 124 130 A G S <> S- 0 0 8 -3,-1.1 4,-2.2 1,-0.1 5,-0.2 -0.175 86.5-102.3 -76.3 167.9 -0.7 23.2 35.5 125 131 A P T 4>S+ 0 0 23 0, 0.0 5,-2.5 0, 0.0 6,-0.5 0.778 117.4 55.4 -61.5 -28.6 -2.8 23.7 32.4 126 132 A V T >45S+ 0 0 0 3,-0.2 -76,-2.5 2,-0.2 3,-1.0 0.970 114.5 34.6 -68.9 -55.2 -3.0 19.9 31.8 127 133 A M T 345S+ 0 0 41 -6,-0.6 -1,-0.2 1,-0.3 -5,-0.1 0.772 118.7 52.8 -75.0 -25.0 -4.5 18.9 35.2 128 134 A Q T 3<5S- 0 0 101 -4,-2.2 -1,-0.3 -7,-0.2 -2,-0.2 0.462 107.0-130.6 -83.7 -3.7 -6.5 22.2 35.4 129 135 A N T < 5 + 0 0 53 -3,-1.0 -3,-0.2 -4,-0.2 -2,-0.1 0.871 68.7 127.5 56.2 42.8 -7.9 21.3 31.9 130 136 A K < + 0 0 129 -5,-2.5 2,-0.2 -6,-0.2 -4,-0.2 -0.033 28.1 131.0-118.4 29.0 -7.1 24.8 30.6 131 137 A A - 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