==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER OXIDOREDUCTASE 13-JUL-05 2CZC . COMPND 2 MOLECULE: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; . SOURCE 2 ORGANISM_SCIENTIFIC: PYROCOCCUS HORIKOSHII; . AUTHOR K.ITO,R.ARAI,T.KAMO-UCHIKUBO,M.SHIROUZU,S.YOKOYAMA,RIKEN STR . 1332 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 44897.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1036 77.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 177 13.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 116 8.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 117 8.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 199 14.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 346 26.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 28 2.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 6 3 3 4 4 4 9 7 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 16 8 3 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 8 4 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A M 0 0 208 0, 0.0 2,-0.2 0, 0.0 3,-0.0 0.000 360.0 360.0 360.0 165.2 29.9 25.5 20.2 2 2 A K - 0 0 88 25,-0.1 25,-0.3 1,-0.1 2,-0.2 -0.552 360.0-110.1 -82.7 145.0 29.9 23.4 17.1 3 3 A V E -a 27 0A 9 23,-2.5 25,-2.5 -2,-0.2 2,-0.7 -0.579 35.2-123.4 -72.3 136.6 32.5 20.8 16.4 4 4 A K E -a 28 0A 54 -2,-0.2 77,-2.6 23,-0.2 78,-1.8 -0.766 33.4-167.9 -89.7 116.6 34.7 22.0 13.6 5 5 A V E -ab 29 82A 0 23,-2.9 25,-3.1 -2,-0.7 26,-0.9 -0.819 14.4-174.1-110.9 143.6 34.8 19.4 10.8 6 6 A G E -ab 31 83A 0 76,-1.8 78,-2.9 -2,-0.4 2,-0.5 -0.949 14.9-146.0-126.9 147.6 37.0 19.1 7.7 7 7 A V E -ab 32 84A 0 24,-2.4 26,-1.7 -2,-0.3 2,-0.7 -0.963 5.7-161.5-121.6 119.1 36.5 16.5 4.9 8 8 A N E S+ b 0 85A 5 76,-2.8 78,-3.5 -2,-0.5 3,-0.1 -0.880 75.3 28.9 -98.2 114.5 39.6 15.1 3.2 9 9 A G - 0 0 15 -2,-0.7 2,-1.8 24,-0.3 5,-0.2 0.781 67.1-153.0 99.6 84.8 38.5 13.6 -0.1 10 10 A Y + 0 0 10 23,-1.6 -1,-0.1 4,-0.2 34,-0.1 -0.404 53.4 127.1 -85.8 59.3 35.4 15.2 -1.6 11 11 A G S > S- 0 0 29 -2,-1.8 4,-2.6 75,-0.4 5,-0.4 0.043 80.6 -57.3 -97.4-152.0 34.4 12.0 -3.5 12 12 A T H > S+ 0 0 48 1,-0.2 4,-0.6 2,-0.2 -2,-0.0 0.922 141.2 28.7 -61.9 -41.0 31.2 10.0 -3.6 13 13 A I H > S+ 0 0 31 73,-0.2 4,-2.2 2,-0.1 3,-0.2 0.913 120.1 56.4 -82.3 -44.5 31.2 9.5 0.2 14 14 A G H > S+ 0 0 0 72,-0.4 4,-2.8 1,-0.2 5,-0.2 0.904 105.6 46.9 -55.2 -49.9 33.0 12.7 1.0 15 15 A K H X S+ 0 0 2 -4,-2.6 4,-1.8 1,-0.2 -1,-0.2 0.847 113.3 51.1 -65.7 -28.5 30.7 15.2 -0.6 16 16 A R H X S+ 0 0 0 -4,-0.6 4,-2.0 -5,-0.4 -1,-0.2 0.830 111.1 47.0 -76.0 -33.2 27.7 13.5 0.9 17 17 A V H X S+ 0 0 0 -4,-2.2 4,-3.1 2,-0.2 -2,-0.2 0.926 109.9 53.7 -71.1 -43.2 29.2 13.6 4.4 18 18 A A H X S+ 0 0 0 -4,-2.8 4,-1.9 1,-0.2 -2,-0.2 0.905 109.9 48.5 -56.0 -43.8 30.1 17.3 3.9 19 19 A Y H X S+ 0 0 47 -4,-1.8 4,-0.9 -5,-0.2 -1,-0.2 0.934 110.4 51.0 -62.3 -46.2 26.5 17.9 3.0 20 20 A A H >< S+ 0 0 0 -4,-2.0 3,-1.0 1,-0.2 4,-0.4 0.921 107.1 53.9 -58.5 -43.4 25.4 16.0 6.1 21 21 A V H >< S+ 0 0 0 -4,-3.1 3,-1.7 1,-0.3 -1,-0.2 0.904 102.9 56.3 -59.8 -39.8 27.7 18.0 8.3 22 22 A T H 3< S+ 0 0 72 -4,-1.9 -1,-0.3 1,-0.3 -2,-0.2 0.764 102.9 57.8 -64.1 -20.7 26.2 21.3 7.0 23 23 A K T << S+ 0 0 86 -3,-1.0 2,-0.4 -4,-0.9 -1,-0.3 0.499 88.1 91.4 -87.1 -3.1 22.7 20.0 8.1 24 24 A Q X - 0 0 9 -3,-1.7 3,-0.9 -4,-0.4 302,-0.0 -0.779 62.9-154.9 -95.9 132.8 23.8 19.5 11.7 25 25 A D T 3 S+ 0 0 107 -2,-0.4 -1,-0.1 1,-0.2 -3,-0.0 0.556 95.0 52.3 -81.9 -4.9 23.3 22.4 14.1 26 26 A D T 3 S+ 0 0 32 -5,-0.1 -23,-2.5 -24,-0.1 2,-0.3 0.157 106.6 59.2-112.1 15.8 26.1 21.3 16.4 27 27 A M E < -a 3 0A 4 -3,-0.9 2,-0.4 -25,-0.3 -23,-0.2 -0.983 62.1-154.6-142.0 153.1 28.8 21.0 13.7 28 28 A E E -a 4 0A 88 -25,-2.5 -23,-2.9 -2,-0.3 2,-1.0 -0.996 18.5-131.7-132.6 132.5 30.4 23.3 11.2 29 29 A L E -a 5 0A 21 -2,-0.4 -23,-0.2 1,-0.2 3,-0.1 -0.703 23.1-179.6 -84.1 103.8 32.0 22.4 7.9 30 30 A I E - 0 0 29 -25,-3.1 2,-0.3 -2,-1.0 -24,-0.2 0.825 58.3 -58.5 -72.2 -33.9 35.4 24.2 7.9 31 31 A G E -a 6 0A 0 -26,-0.9 -24,-2.4 -3,-0.1 2,-0.3 -0.914 41.8-123.3 167.1 168.4 36.3 22.9 4.4 32 32 A I E -ac 7 51A 0 18,-2.3 20,-3.0 -2,-0.3 2,-0.3 -0.872 22.4-126.7-130.4 164.2 36.9 20.0 2.0 33 33 A T E - c 0 52A 1 -26,-1.7 -23,-1.6 -2,-0.3 -24,-0.3 -0.839 18.4-176.5-114.9 153.8 39.8 18.9 -0.2 34 34 A K E - c 0 53A 21 18,-1.9 20,-2.6 -2,-0.3 21,-0.3 -0.960 24.6-145.6-139.1 154.5 40.1 18.1 -3.8 35 35 A T S S+ 0 0 52 -2,-0.3 26,-0.2 1,-0.2 27,-0.1 0.545 86.5 19.3 -97.6 -8.0 43.0 16.8 -5.9 36 36 A K S S- 0 0 90 25,-0.1 2,-1.8 26,-0.0 -1,-0.2 -0.994 79.2-109.7-158.4 152.4 42.0 18.7 -9.0 37 37 A P + 0 0 14 0, 0.0 2,-0.1 0, 0.0 16,-0.0 -0.409 63.8 143.0 -84.4 64.4 39.8 21.7 -10.1 38 38 A D S >> S- 0 0 20 -2,-1.8 4,-2.0 548,-0.1 3,-0.5 -0.216 71.4 -77.0 -90.6-171.9 37.1 19.6 -11.8 39 39 A F H 3> S+ 0 0 18 546,-2.9 4,-1.6 1,-0.2 547,-0.1 0.794 130.6 55.8 -58.9 -29.7 33.3 20.3 -11.8 40 40 A E H 3> S+ 0 0 18 545,-0.2 4,-2.3 2,-0.2 -1,-0.2 0.884 106.2 50.8 -70.6 -37.7 32.9 18.9 -8.3 41 41 A A H <> S+ 0 0 0 -3,-0.5 4,-2.1 1,-0.2 -2,-0.2 0.931 110.0 49.9 -63.5 -45.6 35.5 21.4 -7.0 42 42 A Y H X S+ 0 0 79 -4,-2.0 4,-2.5 1,-0.2 -1,-0.2 0.855 109.1 53.4 -61.0 -33.1 33.6 24.2 -8.7 43 43 A R H X S+ 0 0 34 -4,-1.6 4,-2.0 2,-0.2 -2,-0.2 0.923 105.2 52.2 -68.7 -43.7 30.4 22.9 -7.1 44 44 A A H <>S+ 0 0 0 -4,-2.3 5,-2.4 2,-0.2 4,-0.2 0.953 113.5 44.4 -56.6 -49.4 31.9 23.0 -3.6 45 45 A K H ><5S+ 0 0 88 -4,-2.1 3,-2.4 1,-0.2 -1,-0.2 0.928 109.2 55.7 -60.6 -44.8 33.0 26.6 -4.1 46 46 A E H 3<5S+ 0 0 119 -4,-2.5 -1,-0.2 1,-0.3 -2,-0.2 0.845 106.0 54.1 -56.0 -32.4 29.6 27.4 -5.7 47 47 A L T 3<5S- 0 0 86 -4,-2.0 -1,-0.3 -3,-0.2 -2,-0.2 0.487 121.3-110.1 -80.4 -4.1 28.2 26.1 -2.4 48 48 A G T < 5S+ 0 0 64 -3,-2.4 -3,-0.2 1,-0.3 -2,-0.1 0.554 72.6 141.3 86.2 6.1 30.3 28.4 -0.3 49 49 A I < - 0 0 18 -5,-2.4 -1,-0.3 -6,-0.2 -2,-0.1 -0.727 58.2-115.6 -86.7 124.6 32.4 25.5 1.0 50 50 A P - 0 0 44 0, 0.0 -18,-2.3 0, 0.0 2,-0.6 -0.372 28.9-146.0 -61.3 128.5 36.1 26.4 1.3 51 51 A V E -c 32 0A 5 -20,-0.2 19,-2.4 17,-0.2 20,-0.5 -0.885 15.8-172.3-104.0 120.7 38.1 24.2 -1.1 52 52 A Y E -c 33 0A 12 -20,-3.0 -18,-1.9 -2,-0.6 20,-0.3 -0.888 29.8-114.3-110.6 140.0 41.6 23.1 -0.1 53 53 A A E -cd 34 72A 0 18,-2.9 20,-1.3 -2,-0.4 -18,-0.2 -0.563 27.5-125.4 -70.8 134.9 44.1 21.3 -2.4 54 54 A A S S- 0 0 18 -20,-2.6 2,-0.3 -2,-0.3 -1,-0.2 0.713 85.7 -11.0 -53.6 -21.5 44.6 17.8 -0.9 55 55 A S S >> S- 0 0 21 -21,-0.3 3,-1.8 16,-0.1 4,-0.7 -0.980 70.6 -99.0-171.5 162.9 48.3 18.6 -0.9 56 56 A E G >4 S+ 0 0 97 -2,-0.3 3,-0.7 1,-0.3 -1,-0.1 0.822 118.2 67.2 -58.9 -28.8 51.0 21.0 -2.0 57 57 A E G 34 S+ 0 0 148 1,-0.2 -1,-0.3 2,-0.1 4,-0.1 0.718 102.6 43.0 -64.3 -23.7 51.6 18.4 -4.8 58 58 A F G X> S+ 0 0 58 -3,-1.8 3,-1.3 1,-0.1 4,-1.0 0.521 85.7 95.1-101.0 -7.7 48.2 19.1 -6.4 59 59 A I H S+ 0 0 66 0, 0.0 4,-2.9 0, 0.0 -1,-0.3 0.861 97.2 58.6 -62.2 -31.9 49.8 23.2 -9.6 61 61 A R H <> S+ 0 0 113 -3,-1.3 4,-1.2 2,-0.2 -2,-0.2 0.899 112.1 41.0 -62.3 -39.0 46.6 21.7 -11.1 62 62 A F H X>S+ 0 0 2 -4,-1.0 5,-3.1 -3,-0.3 4,-0.6 0.916 114.6 49.5 -73.9 -45.8 44.6 24.5 -9.5 63 63 A E H ><5S+ 0 0 141 -4,-2.7 3,-1.0 3,-0.2 -2,-0.2 0.903 106.7 58.9 -59.4 -42.1 47.1 27.3 -10.3 64 64 A K H 3<5S+ 0 0 171 -4,-2.9 -1,-0.2 1,-0.3 -2,-0.2 0.906 112.5 37.4 -53.7 -47.1 47.1 26.0 -13.9 65 65 A E H 3<5S- 0 0 97 -4,-1.2 -1,-0.3 -5,-0.2 -2,-0.2 0.462 113.8-117.1 -84.5 -2.3 43.3 26.5 -14.2 66 66 A G T <<5 + 0 0 65 -3,-1.0 2,-0.8 -4,-0.6 -3,-0.2 0.830 60.8 155.8 69.6 30.6 43.5 29.7 -12.1 67 67 A F < - 0 0 10 -5,-3.1 -1,-0.2 -6,-0.1 2,-0.2 -0.818 36.8-140.0 -95.2 107.7 41.3 28.1 -9.5 68 68 A E - 0 0 161 -2,-0.8 2,-0.3 -3,-0.1 -17,-0.2 -0.431 19.7-167.2 -70.0 134.3 41.9 29.8 -6.1 69 69 A V - 0 0 34 -2,-0.2 -17,-0.2 -19,-0.1 3,-0.1 -0.822 30.4-126.5-119.1 160.2 42.1 27.5 -3.0 70 70 A A S S- 0 0 55 -19,-2.4 2,-0.3 -2,-0.3 -18,-0.2 0.448 74.3 -77.7 -82.6 -1.3 42.0 28.3 0.7 71 71 A G - 0 0 13 -20,-0.5 -18,-2.9 4,-0.1 -1,-0.2 -0.964 60.2 -56.0 141.3-158.0 45.2 26.4 1.1 72 72 A T B >> -d 53 0A 17 -2,-0.3 4,-2.1 -20,-0.3 3,-1.1 -0.652 57.7 -90.7-114.9 173.9 46.3 22.7 1.4 73 73 A L H 3> S+ 0 0 4 -20,-1.3 4,-2.9 1,-0.3 5,-0.2 0.846 126.3 62.3 -55.1 -34.9 45.1 20.0 3.8 74 74 A N H 3> S+ 0 0 68 1,-0.2 4,-0.6 2,-0.2 -1,-0.3 0.891 105.4 46.0 -59.6 -36.4 47.9 21.1 6.1 75 75 A D H X4 S+ 0 0 63 -3,-1.1 3,-1.1 1,-0.2 4,-0.4 0.942 112.4 50.3 -70.1 -45.2 46.2 24.4 6.4 76 76 A L H >< S+ 0 0 0 -4,-2.1 3,-1.5 1,-0.2 -2,-0.2 0.861 103.0 60.2 -60.5 -37.8 42.8 22.8 6.9 77 77 A L H >< S+ 0 0 5 -4,-2.9 3,-0.5 1,-0.3 -1,-0.2 0.719 98.1 59.6 -65.1 -20.7 44.2 20.5 9.7 78 78 A E T << S+ 0 0 160 -3,-1.1 -1,-0.3 -4,-0.6 -2,-0.2 0.627 109.0 44.0 -82.3 -12.3 45.1 23.5 11.7 79 79 A K T < S+ 0 0 103 -3,-1.5 2,-0.3 -4,-0.4 -1,-0.2 0.245 100.4 74.8-118.9 19.1 41.5 24.7 11.8 80 80 A V < - 0 0 10 -3,-0.5 -75,-0.2 -4,-0.2 3,-0.1 -0.898 57.3-150.6-128.8 158.1 39.4 21.6 12.5 81 81 A D S S+ 0 0 73 -77,-2.6 24,-1.8 1,-0.3 2,-0.3 0.698 89.5 15.3 -92.6 -27.0 38.7 19.5 15.6 82 82 A I E -be 5 105A 0 -78,-1.8 -76,-1.8 22,-0.2 2,-0.5 -0.999 64.2-146.5-150.4 147.2 38.2 16.4 13.4 83 83 A I E -be 6 106A 0 22,-2.1 24,-2.3 -2,-0.3 2,-0.7 -0.952 10.0-148.2-116.3 130.4 38.9 15.2 9.9 84 84 A V E -be 7 107A 0 -78,-2.9 -76,-2.8 -2,-0.5 2,-0.7 -0.900 18.8-149.8 -94.8 115.7 36.6 12.9 8.1 85 85 A D E +b 8 0A 1 22,-3.5 24,-0.4 -2,-0.7 -76,-0.2 -0.806 29.6 162.3 -90.8 115.4 38.9 10.9 5.8 86 86 A A + 0 0 15 -78,-3.5 -72,-0.4 -2,-0.7 -75,-0.4 -0.188 30.4 128.4-124.8 40.2 37.0 9.9 2.7 87 87 A T - 0 0 24 1,-0.2 5,-0.2 -74,-0.1 22,-0.2 -0.368 69.4 -64.1 -90.1 171.1 40.0 9.1 0.4 88 88 A P S >> S- 0 0 94 0, 0.0 3,-2.4 0, 0.0 4,-0.6 -0.164 70.5 -79.8 -55.4 149.1 40.6 5.9 -1.6 89 89 A G T 34 S+ 0 0 54 1,-0.3 23,-0.2 2,-0.2 21,-0.1 -0.217 121.3 21.8 -50.9 131.5 41.0 2.6 0.4 90 90 A G T 3> S+ 0 0 36 -3,-0.1 4,-0.9 23,-0.0 -1,-0.3 -0.012 104.5 85.9 97.5 -29.8 44.5 2.3 1.7 91 91 A I H <> S+ 0 0 58 -3,-2.4 4,-1.7 1,-0.2 -2,-0.2 0.771 78.4 65.1 -74.5 -25.6 45.0 6.1 1.5 92 92 A G H X S+ 0 0 0 -4,-0.6 4,-2.0 1,-0.2 3,-0.3 0.943 98.5 52.8 -61.5 -46.0 43.5 6.6 4.9 93 93 A A H 4 S+ 0 0 54 1,-0.2 -1,-0.2 2,-0.2 -2,-0.2 0.883 108.1 51.8 -56.3 -39.2 46.3 4.7 6.6 94 94 A K H < S+ 0 0 153 -4,-0.9 4,-0.3 1,-0.2 -1,-0.2 0.848 110.6 47.3 -69.4 -32.6 48.8 6.9 4.8 95 95 A N H >X S+ 0 0 23 -4,-1.7 3,-1.8 -3,-0.3 4,-1.5 0.792 89.7 85.7 -78.2 -28.1 47.1 10.1 6.1 96 96 A K H 3X S+ 0 0 40 -4,-2.0 4,-2.7 1,-0.3 3,-0.2 0.832 86.0 52.5 -43.0 -49.6 46.7 8.9 9.7 97 97 A P H 3> S+ 0 0 75 0, 0.0 4,-2.3 0, 0.0 -1,-0.3 0.823 106.7 54.8 -61.6 -26.8 50.2 10.1 10.8 98 98 A L H <> S+ 0 0 67 -3,-1.8 4,-1.5 -4,-0.3 -2,-0.2 0.888 110.4 46.3 -70.6 -36.8 49.4 13.5 9.4 99 99 A Y H X>S+ 0 0 0 -4,-1.5 5,-1.8 -3,-0.2 4,-0.6 0.911 112.9 48.4 -71.1 -43.0 46.3 13.6 11.5 100 100 A E H ><5S+ 0 0 108 -4,-2.7 3,-0.7 1,-0.2 -2,-0.2 0.904 111.6 50.2 -64.8 -39.8 48.1 12.4 14.7 101 101 A K H 3<5S+ 0 0 188 -4,-2.3 -1,-0.2 1,-0.2 -2,-0.2 0.858 111.6 48.3 -66.5 -33.6 50.9 15.0 14.1 102 102 A A H 3<5S- 0 0 45 -4,-1.5 -1,-0.2 -5,-0.1 -2,-0.2 0.537 109.6-127.7 -82.9 -7.4 48.3 17.7 13.7 103 103 A G T <<5 + 0 0 65 -3,-0.7 2,-0.3 -4,-0.6 -3,-0.2 0.730 61.9 132.8 69.9 24.2 46.5 16.5 16.9 104 104 A V < - 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