==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=29-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER ISOMERASE 30-MAR-06 2DJ2 . COMPND 2 MOLECULE: PROTEIN DISULFIDE-ISOMERASE A4; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR N.TOCHIO,S.KOSHIBA,M.INOUE,T.KIGAWA,S.YOKOYAMA,RIKEN . 120 1 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 7295.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 80 66.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 8 6.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 10 8.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 1 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 13 10.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 12 10.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 34 28.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 1 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G 0 0 137 0, 0.0 2,-0.7 0, 0.0 15,-0.0 0.000 360.0 360.0 360.0 -51.6 -17.6 -4.7 -10.3 2 2 A S + 0 0 76 9,-0.0 2,-0.3 14,-0.0 3,-0.1 -0.882 360.0 138.5-111.6 102.7 -14.4 -2.6 -10.6 3 3 A S + 0 0 126 -2,-0.7 0, 0.0 1,-0.1 0, 0.0 -0.859 27.7 92.3-148.0 108.0 -12.2 -3.8 -13.4 4 4 A G + 0 0 80 -2,-0.3 2,-0.4 1,-0.1 -1,-0.1 0.207 46.7 119.9 173.4 41.7 -10.3 -1.4 -15.8 5 5 A S - 0 0 110 -3,-0.1 2,-1.1 2,-0.1 -1,-0.1 -0.981 46.2-151.4-125.7 127.0 -6.8 -0.6 -14.5 6 6 A S + 0 0 138 -2,-0.4 2,-0.3 2,-0.0 3,-0.1 -0.703 63.0 71.9 -97.2 84.4 -3.6 -1.3 -16.4 7 7 A G S S- 0 0 58 -2,-1.1 -2,-0.1 1,-0.1 40,-0.0 -0.946 82.3-101.3-169.5-172.2 -1.1 -1.8 -13.6 8 8 A V S S+ 0 0 38 -2,-0.3 54,-0.3 39,-0.1 2,-0.1 0.818 91.9 80.3 -96.3 -40.8 0.3 -4.0 -10.8 9 9 A T S S- 0 0 13 52,-0.1 2,-0.4 38,-0.1 54,-0.3 -0.434 73.0-140.0 -70.7 141.0 -1.2 -2.2 -7.8 10 10 A L B -a 63 0A 72 52,-1.8 54,-2.6 -2,-0.1 2,-0.9 -0.842 5.9-134.5-106.1 140.2 -4.9 -2.9 -7.0 11 11 A S - 0 0 60 -2,-0.4 2,-0.4 52,-0.2 54,-0.2 -0.809 24.3-169.6 -96.4 104.8 -7.4 -0.2 -5.9 12 12 A L - 0 0 3 -2,-0.9 54,-0.3 52,-0.4 2,-0.3 -0.799 3.7-170.6 -97.4 133.0 -9.4 -1.5 -2.9 13 13 A T >> - 0 0 31 -2,-0.4 3,-1.6 52,-0.1 4,-0.9 -0.806 42.3-102.2-119.8 161.4 -12.4 0.4 -1.7 14 14 A K T 34 S+ 0 0 109 1,-0.3 4,-0.2 -2,-0.3 -1,-0.0 0.657 126.6 53.2 -54.1 -13.6 -14.7 0.1 1.3 15 15 A D T 34 S+ 0 0 132 1,-0.1 -1,-0.3 2,-0.1 -3,-0.0 0.738 127.7 16.9 -92.7 -28.2 -17.0 -1.6 -1.2 16 16 A N T <4 S+ 0 0 24 -3,-1.6 4,-0.4 3,-0.1 -2,-0.2 -0.076 100.3 96.2-135.0 33.3 -14.5 -4.1 -2.4 17 17 A F S X S+ 0 0 1 -4,-0.9 4,-1.6 -5,-0.2 5,-0.3 0.871 93.7 33.3 -89.6 -44.8 -11.9 -4.0 0.4 18 18 A D T 4 S+ 0 0 80 -5,-0.2 4,-0.2 -4,-0.2 -1,-0.1 0.515 119.3 55.5 -88.2 -6.8 -13.0 -6.9 2.5 19 19 A D T 4 S+ 0 0 102 3,-0.1 -1,-0.2 2,-0.1 -2,-0.2 0.691 117.0 32.6 -95.9 -24.5 -14.2 -8.7 -0.6 20 20 A V T >4 S+ 0 0 55 -4,-0.4 3,-0.7 2,-0.1 -2,-0.2 0.853 118.6 48.9 -97.1 -48.7 -10.9 -8.6 -2.4 21 21 A V G >< S+ 0 0 4 -4,-1.6 3,-2.1 1,-0.3 69,-0.4 0.897 108.0 56.1 -59.0 -42.2 -8.4 -8.8 0.5 22 22 A N G 3 S+ 0 0 63 1,-0.3 -1,-0.3 -5,-0.3 -2,-0.1 0.775 106.6 51.6 -61.7 -26.0 -10.2 -11.8 2.0 23 23 A N G < S+ 0 0 124 -3,-0.7 2,-0.6 1,-0.1 -1,-0.3 0.179 94.5 87.6 -96.0 16.0 -9.8 -13.5 -1.4 24 24 A A < - 0 0 15 -3,-2.1 66,-1.1 1,-0.2 3,-0.4 -0.890 66.6-153.9-121.0 101.1 -6.0 -12.8 -1.3 25 25 A D S S+ 0 0 116 -2,-0.6 65,-0.3 1,-0.2 2,-0.3 0.849 91.0 4.1 -34.9 -50.3 -3.9 -15.4 0.5 26 26 A I S S+ 0 0 20 63,-0.2 2,-0.3 -3,-0.1 -1,-0.2 -0.867 80.8 156.8-147.1 108.6 -1.4 -12.6 1.1 27 27 A I E -B 88 0A 1 61,-2.2 61,-2.9 -3,-0.4 2,-0.5 -0.950 27.2-143.1-132.5 152.4 -1.9 -8.9 0.2 28 28 A L E -Bc 87 62A 0 33,-1.0 35,-2.4 -2,-0.3 2,-0.4 -0.962 10.9-156.0-119.7 128.7 -0.5 -5.6 1.4 29 29 A V E -Bc 86 63A 0 57,-2.9 57,-2.5 -2,-0.5 2,-0.5 -0.863 3.6-165.7-105.6 134.7 -2.6 -2.4 1.7 30 30 A E E -Bc 85 64A 4 33,-1.9 35,-1.2 -2,-0.4 2,-0.6 -0.915 11.6-148.4-123.3 105.2 -1.0 1.1 1.6 31 31 A F E +Bc 84 65A 0 53,-3.3 53,-1.6 -2,-0.5 2,-0.3 -0.599 30.2 167.5 -74.6 115.4 -3.2 4.0 2.7 32 32 A Y E - c 0 66A 36 33,-2.4 35,-1.1 -2,-0.6 36,-0.6 -0.813 27.2-134.7-125.9 166.5 -2.1 7.1 0.7 33 33 A A > - 0 0 0 -2,-0.3 3,-0.6 3,-0.2 7,-0.1 -0.979 13.2-139.6-127.6 136.0 -3.6 10.5 0.1 34 34 A P T 3 S+ 0 0 81 0, 0.0 -1,-0.1 0, 0.0 32,-0.0 0.426 110.5 40.5 -69.8 3.2 -3.9 12.5 -3.2 35 35 A W T 3 S+ 0 0 168 0, 0.0 2,-0.5 0, 0.0 -3,-0.0 0.484 88.7 103.9-124.9 -15.2 -2.8 15.6 -1.2 36 36 A C <> - 0 0 9 -3,-0.6 4,-1.1 1,-0.2 -3,-0.2 -0.607 54.5-159.5 -76.1 120.1 -0.1 14.1 1.0 37 37 A G H >> S+ 0 0 47 -2,-0.5 3,-1.9 1,-0.2 4,-0.7 0.990 87.3 59.4 -62.0 -63.9 3.4 15.0 -0.2 38 38 A H H >> S+ 0 0 125 1,-0.3 3,-1.4 2,-0.2 4,-1.0 0.805 102.8 58.7 -33.5 -40.8 5.4 12.3 1.4 39 39 A C H >> S+ 0 0 7 1,-0.3 4,-3.2 2,-0.2 3,-1.2 0.928 87.4 71.0 -58.5 -47.8 3.2 9.9 -0.5 40 40 A K H << S+ 0 0 110 -3,-1.9 -1,-0.3 -4,-1.1 -2,-0.2 0.738 103.8 46.2 -41.7 -24.1 4.2 11.4 -3.9 41 41 A K H S+ 0 0 52 0, 0.0 4,-1.5 0, 0.0 5,-0.3 0.810 116.0 57.6 -69.8 -31.1 6.6 5.1 -7.2 45 45 A E H X S+ 0 0 58 -4,-0.8 4,-0.7 -3,-0.2 -2,-0.2 0.732 116.1 36.6 -71.9 -22.4 8.2 3.1 -4.4 46 46 A Y H X S+ 0 0 0 -4,-2.8 4,-1.8 -3,-0.4 -3,-0.2 0.896 115.7 48.3 -93.5 -56.1 4.9 1.2 -4.0 47 47 A E H X S+ 0 0 61 -4,-2.7 4,-0.7 -5,-0.3 -2,-0.2 0.866 116.1 48.1 -53.2 -38.6 3.7 0.7 -7.6 48 48 A K H >X S+ 0 0 97 -4,-1.5 3,-2.1 -5,-0.3 4,-1.3 0.970 105.5 54.4 -67.4 -55.7 7.2 -0.4 -8.5 49 49 A A H 3X S+ 0 0 0 -4,-0.7 4,-2.2 1,-0.3 5,-0.2 0.813 102.0 62.3 -48.1 -32.8 7.7 -2.9 -5.6 50 50 A A H 3X S+ 0 0 1 -4,-1.8 4,-2.7 1,-0.2 -1,-0.3 0.838 99.5 53.6 -63.5 -33.6 4.4 -4.5 -6.9 51 51 A K H > - c 0 32A 36 -2,-0.4 4,-3.4 -54,-0.3 3,-1.1 -0.763 11.7-165.6 -91.2 102.4 -7.1 6.2 -0.5 67 67 A A T 34 S+ 0 0 1 -35,-1.1 -1,-0.2 -2,-1.0 -34,-0.1 0.595 87.8 66.3 -61.2 -8.5 -7.6 6.9 3.2 68 68 A T T 34 S+ 0 0 45 -36,-0.6 -1,-0.3 2,-0.1 3,-0.2 0.865 116.4 20.6 -80.9 -39.2 -9.2 10.1 2.0 69 69 A E T <4 S+ 0 0 128 -3,-1.1 3,-0.3 1,-0.1 -2,-0.2 0.740 128.5 49.3 -98.9 -31.2 -12.2 8.4 0.3 70 70 A Q >X + 0 0 18 -4,-3.4 3,-2.0 1,-0.2 4,-1.7 -0.117 66.7 141.2-100.0 35.2 -12.1 5.1 2.2 71 71 A T H 3> + 0 0 61 1,-0.3 4,-2.7 -3,-0.2 5,-0.4 0.862 65.6 65.9 -42.6 -44.3 -11.9 6.8 5.6 72 72 A D H 3> S+ 0 0 101 -3,-0.3 4,-1.1 1,-0.2 -1,-0.3 0.875 105.8 42.6 -47.1 -43.1 -14.1 4.1 7.0 73 73 A L H <> S+ 0 0 10 -3,-2.0 4,-1.1 2,-0.2 -1,-0.2 0.906 111.6 54.7 -71.8 -43.0 -11.4 1.6 6.3 74 74 A A H ><>S+ 0 0 4 -4,-1.7 3,-1.8 1,-0.2 5,-1.8 0.963 106.4 49.6 -54.5 -58.3 -8.6 3.9 7.6 75 75 A K H ><5S+ 0 0 161 -4,-2.7 3,-1.8 1,-0.3 -1,-0.2 0.847 104.7 61.1 -50.5 -36.8 -10.3 4.4 11.0 76 76 A R H 3<5S+ 0 0 147 -4,-1.1 -1,-0.3 -5,-0.4 -2,-0.2 0.823 111.6 38.2 -61.0 -31.6 -10.7 0.6 11.2 77 77 A F T <<5S- 0 0 14 -3,-1.8 -1,-0.3 -4,-1.1 -2,-0.2 0.035 116.3-113.0-107.3 24.3 -6.9 0.4 11.1 78 78 A D T < 5 + 0 0 144 -3,-1.8 2,-1.0 1,-0.2 -3,-0.2 0.896 50.8 177.9 44.5 49.5 -6.3 3.4 13.2 79 79 A V < + 0 0 13 -5,-1.8 -1,-0.2 -8,-0.2 3,-0.1 -0.729 20.5 145.0 -87.8 101.0 -4.8 5.2 10.2 80 80 A S + 0 0 121 -2,-1.0 -1,-0.1 1,-0.2 2,-0.1 -0.196 63.8 38.8-128.3 40.6 -3.9 8.7 11.4 81 81 A G S S- 0 0 46 -50,-0.0 -1,-0.2 3,-0.0 -50,-0.1 -0.336 84.5-108.7 170.3 101.8 -0.7 9.4 9.4 82 82 A Y S S+ 0 0 55 -3,-0.1 2,-0.2 -2,-0.1 -50,-0.1 -0.556 89.6 53.5 -92.2 157.3 0.2 8.4 5.8 83 83 A P S S+ 0 0 18 0, 0.0 2,-0.4 0, 0.0 -51,-0.2 0.520 74.4 170.6 -69.8 154.1 1.8 6.8 4.2 84 84 A T E -B 31 0A 20 -53,-1.6 -53,-3.3 -2,-0.2 2,-0.3 -0.880 9.7-173.6-139.2 105.0 0.4 3.8 6.2 85 85 A L E +B 30 0A 7 -2,-0.4 11,-1.0 -55,-0.3 2,-0.3 -0.696 5.2 176.0 -98.2 150.5 1.0 0.2 5.0 86 86 A K E -BD 29 95A 24 -57,-2.5 -57,-2.9 -2,-0.3 2,-0.2 -0.955 24.4-129.0-156.1 133.4 -0.5 -2.9 6.5 87 87 A I E -BD 28 94A 4 7,-2.3 7,-2.3 -2,-0.3 2,-0.4 -0.564 20.3-161.0 -83.6 145.9 -0.3 -6.6 5.7 88 88 A F E -BD 27 93A 3 -61,-2.9 -61,-2.2 5,-0.2 2,-0.6 -0.988 7.1-169.6-132.5 125.5 -3.5 -8.7 5.3 89 89 A R S S- 0 0 115 3,-1.6 -63,-0.2 -2,-0.4 -64,-0.2 -0.915 75.8 -41.6-118.2 106.4 -3.8 -12.5 5.5 90 90 A K S S- 0 0 119 -66,-1.1 -68,-0.2 -2,-0.6 -1,-0.1 0.812 133.7 -30.3 46.3 33.3 -7.1 -14.0 4.4 91 91 A G S S+ 0 0 39 -70,-0.3 -1,-0.2 -67,-0.3 -69,-0.1 0.781 112.2 122.7 97.0 33.5 -8.7 -11.1 6.3 92 92 A R - 0 0 147 2,-0.0 -3,-1.6 0, 0.0 -1,-0.2 -0.866 47.9-151.8-132.6 99.6 -6.0 -10.6 9.0 93 93 A P E -D 88 0A 54 0, 0.0 2,-0.4 0, 0.0 -5,-0.2 -0.400 13.0-173.5 -69.8 142.4 -4.4 -7.1 9.3 94 94 A F E -D 87 0A 98 -7,-2.3 -7,-2.3 -2,-0.1 3,-0.0 -0.936 34.4-107.8-144.0 117.0 -0.9 -6.8 10.6 95 95 A D E -D 86 0A 112 -2,-0.4 2,-0.5 -9,-0.2 -9,-0.2 -0.163 39.5-130.3 -43.8 110.2 1.0 -3.6 11.3 96 96 A Y - 0 0 18 -11,-1.0 2,-0.5 1,-0.0 -11,-0.2 -0.559 30.9-178.9 -72.0 117.8 3.5 -3.4 8.5 97 97 A N + 0 0 110 -2,-0.5 -11,-0.0 -3,-0.0 -1,-0.0 -0.958 35.4 60.8-124.6 115.2 7.0 -2.7 9.9 98 98 A G S S- 0 0 40 -2,-0.5 2,-0.1 10,-0.0 7,-0.1 -0.989 83.9 -14.8 167.1-163.2 10.0 -2.4 7.7 99 99 A P - 0 0 53 0, 0.0 9,-0.1 0, 0.0 -2,-0.0 -0.428 40.3-153.0 -69.7 138.8 11.6 -0.4 4.8 100 100 A R + 0 0 143 -2,-0.1 2,-0.3 4,-0.1 -55,-0.1 -0.038 61.5 109.1-101.1 29.8 9.4 1.9 2.8 101 101 A E S > S- 0 0 121 1,-0.1 4,-2.6 -55,-0.0 5,-0.4 -0.808 82.4-111.0-108.6 149.0 11.6 1.6 -0.3 102 102 A K H > S+ 0 0 39 -2,-0.3 4,-1.6 1,-0.2 5,-0.1 0.895 119.8 36.2 -37.4 -61.2 10.7 -0.2 -3.6 103 103 A Y H > S+ 0 0 165 2,-0.2 4,-2.6 1,-0.1 3,-0.4 0.988 118.7 48.9 -59.2 -64.3 13.3 -2.8 -2.9 104 104 A G H > S+ 0 0 12 1,-0.2 4,-3.1 2,-0.2 5,-0.2 0.916 107.6 53.5 -40.1 -67.0 12.9 -3.0 0.9 105 105 A I H X S+ 0 0 0 -4,-2.6 4,-2.6 1,-0.2 -1,-0.2 0.862 113.1 45.7 -36.7 -51.3 9.1 -3.3 0.7 106 106 A V H X S+ 0 0 5 -4,-1.6 4,-1.9 -3,-0.4 -1,-0.2 0.963 111.7 49.7 -59.9 -55.0 9.5 -6.3 -1.7 107 107 A D H X S+ 0 0 107 -4,-2.6 4,-1.7 2,-0.2 -2,-0.2 0.889 115.1 46.6 -51.4 -43.5 12.3 -8.0 0.4 108 108 A Y H X S+ 0 0 34 -4,-3.1 4,-2.0 -5,-0.3 3,-0.3 0.996 105.9 53.9 -63.4 -66.9 10.1 -7.6 3.5 109 109 A M H X S+ 0 0 0 -4,-2.6 4,-2.2 1,-0.2 -1,-0.2 0.799 108.8 56.2 -37.5 -35.9 6.8 -8.9 2.1 110 110 A I H X S+ 0 0 48 -4,-1.9 4,-1.1 -5,-0.2 -1,-0.2 0.985 104.3 46.8 -63.4 -60.8 8.8 -11.9 1.0 111 111 A E H X S+ 0 0 91 -4,-1.7 4,-1.8 -3,-0.3 5,-0.2 0.765 112.4 56.3 -53.3 -25.3 10.1 -12.8 4.5 112 112 A Q H < S+ 0 0 68 -4,-2.0 -1,-0.2 2,-0.2 -2,-0.2 0.955 98.2 55.6 -72.5 -52.7 6.5 -12.3 5.6 113 113 A S H < S- 0 0 34 -4,-2.2 -1,-0.2 -5,-0.2 -2,-0.2 0.726 142.9 -59.1 -53.2 -20.9 4.9 -14.8 3.3 114 114 A G H < - 0 0 28 -4,-1.1 -2,-0.2 -5,-0.1 -1,-0.2 0.437 49.7-166.4 135.7 76.7 7.3 -17.3 4.8 115 115 A S S < S- 0 0 105 -4,-1.8 -3,-0.1 2,-0.1 -4,-0.1 0.898 80.6 -1.9 -49.7 -46.1 11.1 -16.7 4.5 116 116 A G S S- 0 0 33 -5,-0.2 2,-1.0 1,-0.0 0, 0.0 -0.403 115.1 -31.0-126.9-156.2 11.7 -20.3 5.6 117 117 A P S S+ 0 0 141 0, 0.0 2,-0.2 0, 0.0 -2,-0.1 -0.530 70.0 170.4 -69.7 100.7 9.8 -23.5 6.6 118 118 A S - 0 0 95 -2,-1.0 2,-0.3 -4,-0.1 -4,-0.0 -0.599 23.0-135.4-108.3 170.7 6.7 -22.2 8.4 119 119 A S 0 0 126 -2,-0.2 0, 0.0 0, 0.0 0, 0.0 -0.913 360.0 360.0-127.4 154.2 3.5 -23.9 9.6 120 120 A G 0 0 131 -2,-0.3 -2,-0.1 0, 0.0 0, 0.0 -0.646 360.0 360.0 79.5 360.0 -0.2 -23.1 9.4