==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-MAY-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 03-FEB-12 4DKM . COMPND 2 MOLECULE: AMPHIOXUS GREEN FLUORESCENT PROTEIN, GFPC1A; . SOURCE 2 ORGANISM_SCIENTIFIC: BRANCHIOSTOMA FLORIDAE; . AUTHOR D.D.DEHEYN,E.K.BOMATI . 1704 16 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 67120.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1281 75.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 88 5.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 776 45.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 16 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 40 2.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 8 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 121 7.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 128 7.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 96 5.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 8 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 8 8 0 0 16 16 0 8 8 16 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 8 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A L 0 0 84 0, 0.0 2,-0.1 0, 0.0 59,-0.1 0.000 360.0 360.0 360.0 140.2 -25.5 51.0 -30.4 2 4 A P + 0 0 5 0, 0.0 104,-0.2 0, 0.0 3,-0.1 -0.389 360.0 161.7 -70.1 140.4 -23.0 51.2 -33.3 3 5 A T + 0 0 78 102,-3.5 23,-1.9 1,-0.5 2,-0.3 0.592 66.5 17.1-127.0 -35.4 -24.3 51.9 -36.8 4 6 A T E +Ab 25 106A 50 101,-2.3 103,-2.5 21,-0.3 -1,-0.5 -0.945 63.0 177.7-139.6 160.0 -21.2 53.0 -38.8 5 7 A H E -Ab 24 107A 0 19,-2.4 19,-3.1 -2,-0.3 2,-0.3 -0.973 13.7-154.1-157.0 167.7 -17.4 52.8 -38.3 6 8 A E E -Ab 23 108A 72 101,-2.1 103,-2.8 -2,-0.3 2,-0.3 -0.967 13.4-153.2-144.5 156.7 -14.0 53.5 -39.8 7 9 A V E -Ab 22 109A 6 15,-2.7 15,-1.7 -2,-0.3 2,-0.4 -0.947 14.7-167.5-136.9 156.2 -10.6 52.0 -39.4 8 10 A H E -Ab 21 110A 50 101,-0.9 103,-2.5 -2,-0.3 2,-0.5 -0.913 15.3-175.1-141.0 107.4 -6.9 52.9 -39.7 9 11 A V E +Ab 20 111A 2 11,-1.9 11,-3.3 -2,-0.4 2,-0.3 -0.910 12.5 163.9-106.9 133.4 -4.6 49.9 -39.7 10 12 A Y E +Ab 19 112A 68 101,-2.4 103,-2.7 -2,-0.5 2,-0.3 -0.969 22.1 81.1-145.6 158.9 -0.8 50.7 -39.7 11 13 A G E -Ab 18 113A 16 7,-1.4 7,-1.8 -2,-0.3 2,-0.3 -0.994 66.2 -71.2 147.2-137.7 2.3 48.7 -39.0 12 14 A S E -Ab 17 114A 23 101,-1.4 103,-2.8 -2,-0.3 2,-0.4 -0.993 14.5-146.0-157.8 160.9 4.4 46.3 -41.0 13 15 A I E > S-Ab 16 115A 4 3,-2.2 3,-1.9 -2,-0.3 103,-0.2 -0.981 91.1 -14.4-132.3 117.3 4.7 42.9 -42.6 14 16 A N T 3 S- 0 0 33 101,-2.6 102,-0.2 -2,-0.4 -1,-0.1 0.886 131.3 -52.4 57.0 39.4 8.1 41.3 -42.7 15 17 A G T 3 S+ 0 0 37 100,-0.6 2,-0.7 1,-0.2 -1,-0.3 0.372 113.9 118.1 80.8 -6.0 9.7 44.7 -41.9 16 18 A V E < -A 13 0A 102 -3,-1.9 -3,-2.2 29,-0.0 -1,-0.2 -0.856 62.5-129.9-100.7 116.1 7.8 46.5 -44.7 17 19 A E E +A 12 0A 135 -2,-0.7 2,-0.3 -5,-0.3 -5,-0.3 -0.183 29.0 177.2 -60.8 150.8 5.5 49.2 -43.5 18 20 A F E -A 11 0A 14 -7,-1.8 -7,-1.4 26,-0.1 2,-0.3 -0.970 12.0-161.1-150.3 158.3 1.9 49.4 -44.7 19 21 A D E -A 10 0A 24 -2,-0.3 21,-2.2 -9,-0.2 22,-0.4 -0.954 4.9-171.9-150.9 126.8 -1.1 51.6 -44.0 20 22 A L E +AC 9 39A 2 -11,-3.3 -11,-1.9 -2,-0.3 2,-0.3 -0.926 3.9 178.8-121.1 144.1 -4.8 51.0 -44.6 21 23 A V E +AC 8 38A 67 17,-2.0 17,-2.9 -2,-0.4 2,-0.3 -0.994 20.7 117.8-141.8 145.2 -7.8 53.4 -44.2 22 24 A G E -AC 7 37A 17 -15,-1.7 -15,-2.7 -2,-0.3 2,-0.3 -0.981 36.1-129.0 175.5 178.2 -11.5 52.9 -44.8 23 25 A S E +AC 6 36A 74 13,-1.1 13,-1.8 -2,-0.3 -17,-0.3 -0.934 22.3 155.4-146.2 168.5 -15.0 52.9 -43.5 24 26 A G E -AC 5 35A 19 -19,-3.1 -19,-2.4 -2,-0.3 2,-0.2 -0.912 27.0-112.0-168.8-165.5 -18.2 50.8 -43.4 25 27 A K E -AC 4 34A 113 9,-1.3 9,-2.5 -21,-0.3 2,-0.3 -0.807 19.6-168.1-139.4 179.2 -21.4 50.0 -41.5 26 28 A G E - C 0 33A 15 -23,-1.9 7,-0.2 -2,-0.2 3,-0.0 -0.984 26.3-117.2-167.5 161.2 -23.0 47.3 -39.5 27 29 A N E >>> - C 0 32A 62 5,-1.9 4,-1.9 -2,-0.3 3,-1.4 -0.876 14.5-165.7-111.0 101.4 -26.2 46.0 -38.0 28 30 A P T 345S+ 0 0 17 0, 0.0 -1,-0.1 0, 0.0 -25,-0.1 0.600 87.6 65.7 -59.9 -8.3 -26.0 45.7 -34.2 29 31 A K T 345S+ 0 0 144 1,-0.1 32,-0.0 3,-0.1 31,-0.0 0.819 119.8 12.8 -83.0 -34.9 -29.2 43.6 -34.4 30 32 A D T <45S- 0 0 85 -3,-1.4 178,-0.4 2,-0.1 -1,-0.1 0.365 98.8-113.2-125.0 2.3 -27.7 40.7 -36.3 31 33 A G T <5 + 0 0 0 -4,-1.9 29,-0.1 29,-0.7 30,-0.1 0.493 63.5 148.0 79.2 1.6 -23.9 41.1 -36.2 32 34 A S E < +C 27 0A 26 -5,-0.5 -5,-1.9 175,-0.2 2,-0.3 -0.373 20.6 176.0 -68.6 152.2 -23.5 41.7 -39.9 33 35 A E E -CD 26 206A 7 173,-2.4 173,-3.7 -7,-0.2 2,-0.3 -0.983 12.5-177.2-159.3 143.0 -20.7 44.1 -40.8 34 36 A E E +CD 25 205A 77 -9,-2.5 -9,-1.3 -2,-0.3 2,-0.3 -0.997 7.9 165.1-146.3 148.8 -19.1 45.4 -44.0 35 37 A I E -CD 24 204A 11 169,-2.6 169,-3.2 -2,-0.3 2,-0.4 -0.994 23.4-145.9-160.1 158.3 -16.2 47.7 -44.8 36 38 A Q E -CD 23 203A 78 -13,-1.8 -13,-1.1 -2,-0.3 2,-0.3 -0.999 19.7-176.6-130.8 130.3 -13.9 48.9 -47.5 37 39 A V E -CD 22 202A 2 165,-2.8 165,-2.8 -2,-0.4 2,-0.4 -0.940 5.9-163.0-128.1 151.3 -10.2 49.8 -46.9 38 40 A K E -CD 21 201A 111 -17,-2.9 -17,-2.0 -2,-0.3 163,-0.2 -0.987 24.2-112.2-136.0 142.8 -7.6 51.1 -49.2 39 41 A S E -C 20 0A 21 161,-2.3 -19,-0.2 -2,-0.4 3,-0.1 -0.431 16.2-166.5 -71.5 146.8 -3.8 51.3 -49.0 40 42 A T S S+ 0 0 83 -21,-2.2 -20,-0.1 -2,-0.1 -1,-0.1 0.185 84.5 53.6-116.1 10.7 -2.1 54.7 -48.6 41 43 A K S S- 0 0 120 1,-0.5 -1,-0.1 -22,-0.4 -21,-0.1 0.001 115.0 -87.9-134.9 29.4 1.4 53.5 -49.3 42 44 A G S S- 0 0 37 -3,-0.1 -1,-0.5 -23,-0.1 -23,-0.2 -0.372 78.7 -13.5 94.4-176.5 1.1 51.7 -52.7 43 45 A P S S- 0 0 100 0, 0.0 2,-0.3 0, 0.0 157,-0.2 -0.294 74.2-109.0 -61.9 144.8 0.2 48.1 -53.5 44 46 A L - 0 0 13 155,-2.6 -26,-0.1 1,-0.1 157,-0.1 -0.588 17.2-151.6 -76.3 130.3 0.2 45.6 -50.6 45 47 A G S S+ 0 0 48 -2,-0.3 2,-0.3 -29,-0.1 -1,-0.1 0.244 74.0 54.8 -87.0 15.5 3.1 43.1 -50.8 46 48 A F S S- 0 0 11 80,-0.1 79,-0.2 81,-0.1 78,-0.1 -0.948 99.1 -71.3-144.3 164.2 1.2 40.4 -49.0 47 49 A S > - 0 0 0 77,-2.3 3,-1.1 -2,-0.3 4,-0.3 -0.270 33.7-143.3 -57.5 132.3 -2.1 38.4 -49.1 48 50 A P G >> S+ 0 0 21 0, 0.0 3,-1.4 0, 0.0 4,-0.7 0.713 90.8 77.8 -70.4 -17.9 -5.1 40.6 -48.2 49 51 A Y G 34 S+ 0 0 34 1,-0.3 3,-0.3 2,-0.2 -2,-0.1 0.741 84.9 59.6 -65.9 -21.8 -6.7 37.6 -46.4 50 52 A I G <4 S+ 0 0 2 -3,-1.1 4,-0.4 1,-0.2 -1,-0.3 0.680 101.0 57.2 -79.7 -12.4 -4.5 38.0 -43.3 51 53 A V T <4 S+ 0 0 8 -3,-1.4 3,-0.3 -4,-0.3 -1,-0.2 0.692 82.2 89.0 -87.6 -17.4 -5.9 41.6 -42.9 52 54 A V S >< S+ 0 0 5 -4,-0.7 3,-1.8 -3,-0.3 -1,-0.2 0.896 91.5 40.8 -44.5 -56.9 -9.5 40.3 -42.7 53 55 A P T 3 S+ 0 0 24 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 0.843 113.1 57.6 -63.4 -30.9 -9.5 39.8 -38.9 54 56 A N T 3 0 0 5 -4,-0.4 -2,-0.2 -3,-0.3 -3,-0.1 0.314 360.0 360.0 -81.1 10.2 -7.6 43.1 -38.6 55 57 A I < 0 0 5 -3,-1.8 -1,-0.2 -4,-0.2 -3,-0.2 0.547 360.0 360.0-133.7 360.0 -10.4 44.9 -40.4 56 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 57 61 A F > 0 0 12 0, 0.0 3,-1.3 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 72.5 -15.6 45.4 -34.8 58 62 A H G > + 0 0 15 1,-0.3 3,-2.2 2,-0.2 13,-0.3 0.556 360.0 88.4 -70.1 -9.8 -17.3 43.0 -32.4 59 63 A Q G 3 S+ 0 0 0 1,-0.3 -1,-0.3 11,-0.2 9,-0.1 0.692 83.1 60.2 -64.4 -13.1 -19.4 45.9 -31.0 60 64 A Y G < S+ 0 0 0 -3,-1.3 -29,-0.7 -29,-0.1 -1,-0.3 0.574 84.5 102.7 -85.9 -10.6 -21.9 45.0 -33.7 61 65 A L < - 0 0 0 -3,-2.2 7,-1.0 -30,-0.1 10,-0.5 -0.615 54.3-168.0 -80.4 117.0 -22.2 41.5 -32.2 62 66 A P E -L 67 0B 26 0, 0.0 148,-0.5 0, 0.0 5,-0.2 -0.583 14.6-130.7 -96.8 168.0 -25.3 40.9 -30.2 63 67 A F E > -L 66 0B 24 3,-3.6 3,-1.0 -2,-0.2 5,-0.1 -0.736 25.7-106.9-111.7 163.4 -25.9 37.9 -27.9 64 68 A P T 3 S+ 0 0 80 0, 0.0 -1,-0.1 0, 0.0 4,-0.0 0.938 120.0 30.1 -54.8 -49.7 -29.0 35.6 -27.9 65 69 A D T 3 S- 0 0 113 2,-0.1 2,-0.3 -3,-0.0 116,-0.0 0.210 136.0 -47.5 -96.8 14.7 -30.3 37.0 -24.6 66 70 A G E < S-L 63 0B 40 -3,-1.0 -3,-3.6 115,-0.0 2,-0.2 -0.992 91.4 -12.7 158.8-149.5 -28.9 40.5 -25.0 67 71 A M E -L 62 0B 69 -2,-0.3 -2,-0.1 -5,-0.2 -8,-0.0 -0.496 57.9-118.9 -86.5 157.0 -25.8 42.4 -25.9 68 72 A S > - 0 0 0 -7,-1.0 4,-2.6 -2,-0.2 5,-0.3 -0.517 34.3-107.8 -83.8 160.3 -22.3 41.0 -26.2 69 73 A P H > S+ 0 0 3 0, 0.0 4,-1.7 0, 0.0 5,-0.1 0.855 122.5 52.9 -60.1 -30.7 -19.8 42.4 -23.7 70 74 A F H > S+ 0 0 0 2,-0.2 4,-0.9 1,-0.2 -11,-0.2 0.908 111.5 44.6 -68.1 -44.0 -18.2 44.2 -26.7 71 75 A Q H > S+ 0 0 4 -10,-0.5 4,-2.5 -13,-0.3 5,-0.2 0.838 112.2 51.6 -68.9 -36.7 -21.5 45.7 -27.8 72 76 A A H X S+ 0 0 23 -4,-2.6 4,-2.0 1,-0.2 6,-0.2 0.866 109.1 50.9 -68.9 -36.9 -22.5 46.8 -24.2 73 77 A A H X S+ 0 0 7 -4,-1.7 6,-2.6 -5,-0.3 4,-1.2 0.736 112.4 47.9 -72.2 -22.8 -19.1 48.5 -23.8 74 78 A A H < S+ 0 0 10 -4,-0.9 -2,-0.2 2,-0.2 -1,-0.2 0.909 113.2 44.9 -81.1 -46.9 -19.7 50.3 -27.0 75 79 A D H < S+ 0 0 82 -4,-2.5 -2,-0.2 1,-0.2 -3,-0.2 0.836 116.3 50.6 -62.6 -34.0 -23.2 51.4 -26.2 76 80 A D H < S- 0 0 91 -4,-2.0 -1,-0.2 -5,-0.2 -2,-0.2 0.840 101.6-137.4 -71.4 -40.5 -21.8 52.3 -22.8 77 81 A G S < S+ 0 0 45 -4,-1.2 25,-0.2 -5,-0.1 -3,-0.1 0.170 80.6 103.2 96.7 -16.3 -19.0 54.3 -24.2 78 82 A S + 0 0 33 -6,-0.2 -4,-0.2 1,-0.1 -5,-0.1 0.861 62.8 176.5 -65.6 -36.6 -16.6 52.8 -21.7 79 83 A G - 0 0 5 -6,-2.6 95,-1.8 -9,-0.1 96,-0.5 0.013 21.1-111.0 66.2-168.6 -15.1 50.5 -24.4 80 84 A Y E -E 173 0A 15 93,-0.2 20,-0.4 94,-0.1 2,-0.3 -0.974 12.7-123.3-159.7 161.9 -12.3 48.0 -24.4 81 85 A V E -E 172 0A 54 91,-2.4 91,-2.1 -2,-0.3 2,-0.4 -0.824 24.6-139.9-111.2 153.8 -8.8 47.5 -25.8 82 86 A V E -EF 171 98A 2 16,-2.2 16,-2.1 -2,-0.3 2,-0.5 -0.957 15.7-174.3-123.3 131.9 -7.8 44.5 -27.9 83 87 A H E -EF 170 97A 5 87,-2.7 87,-2.9 -2,-0.4 2,-0.4 -0.925 18.0-177.0-121.8 106.5 -4.6 42.4 -27.8 84 88 A R E -EF 169 96A 15 12,-2.9 12,-2.9 -2,-0.5 2,-0.4 -0.855 11.8-172.4-111.4 138.0 -4.5 39.8 -30.6 85 89 A T E -EF 168 95A 9 83,-3.0 83,-2.5 -2,-0.4 2,-0.5 -0.993 2.1-174.5-127.9 125.6 -1.9 37.2 -31.4 86 90 A I E -EF 167 94A 2 8,-2.2 8,-2.3 -2,-0.4 2,-0.5 -0.986 2.8-169.9-120.9 124.6 -2.0 35.0 -34.5 87 91 A Q E -EF 166 93A 44 79,-2.3 79,-1.9 -2,-0.5 2,-0.2 -0.970 10.7-151.7-117.9 121.0 0.5 32.2 -35.0 88 92 A F E > -E 165 0A 5 4,-2.5 3,-2.5 -2,-0.5 77,-0.3 -0.636 26.8-119.9 -92.1 149.8 0.7 30.5 -38.4 89 93 A E T 3 S+ 0 0 56 75,-2.9 76,-0.1 1,-0.3 -1,-0.1 0.763 114.0 69.2 -54.7 -23.6 1.8 26.9 -39.0 90 94 A D T 3 S- 0 0 38 74,-0.4 -1,-0.3 2,-0.1 3,-0.1 0.708 118.7-109.1 -64.8 -30.3 4.4 28.6 -41.0 91 95 A G S < S+ 0 0 15 -3,-2.5 -2,-0.1 1,-0.4 27,-0.1 0.182 76.2 135.7 111.4 -12.2 6.2 30.1 -38.0 92 96 A A - 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