==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-MAY-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 03-FEB-12 4DKN . COMPND 2 MOLECULE: AMPHIOXUS GREEN FLUORESCENT PROTEIN, GFPA1; . SOURCE 2 ORGANISM_SCIENTIFIC: BRANCHIOSTOMA FLORIDAE; . AUTHOR E.K.BOMATI,D.D.DEHEYN . 423 5 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 17450.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 315 74.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 22 5.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 198 46.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 32 7.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 29 6.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 19 4.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 5 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 2 2 0 4 4 0 2 2 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A P 0 0 93 0, 0.0 77,-0.1 0, 0.0 76,-0.0 0.000 360.0 360.0 360.0 171.2 23.1 -2.3 -8.1 2 3 A L - 0 0 51 1,-0.1 59,-0.1 74,-0.1 2,-0.0 -0.351 360.0 -92.1 -70.8 158.0 20.8 -5.2 -7.1 3 4 A P + 0 0 3 0, 0.0 104,-0.2 0, 0.0 -1,-0.1 -0.320 43.0 166.7 -73.9 156.6 17.1 -4.3 -6.6 4 5 A A + 0 0 40 102,-2.8 23,-1.6 1,-0.5 2,-0.3 0.508 67.4 17.5-134.8 -38.0 15.5 -3.4 -3.3 5 6 A T E -Ab 26 107A 57 101,-1.7 103,-2.1 21,-0.2 -1,-0.5 -0.925 61.6-176.9-138.3 162.9 12.1 -1.9 -4.1 6 7 A H E -Ab 25 108A 4 19,-2.4 19,-2.7 -2,-0.3 2,-0.3 -0.989 16.4-145.9-157.0 160.7 9.7 -1.8 -7.1 7 8 A D E -Ab 24 109A 37 101,-2.4 103,-2.0 -2,-0.3 2,-0.3 -0.921 19.4-155.3-127.4 154.9 6.5 -0.5 -8.4 8 9 A I E -Ab 23 110A 3 15,-2.6 15,-2.0 -2,-0.3 2,-0.4 -0.972 14.5-172.7-136.0 147.6 4.1 -2.3 -10.7 9 10 A H E -Ab 22 111A 63 101,-1.3 103,-3.2 -2,-0.3 2,-0.4 -0.924 11.6-175.4-139.6 110.5 1.4 -1.4 -13.3 10 11 A L E +Ab 21 112A 0 11,-2.6 11,-3.0 -2,-0.4 2,-0.3 -0.899 15.7 152.1-109.0 138.2 -0.7 -4.1 -14.7 11 12 A H E +Ab 20 113A 43 101,-2.3 103,-2.4 -2,-0.4 2,-0.3 -0.984 14.3 88.4-158.6 163.1 -3.2 -3.5 -17.5 12 13 A G E -Ab 19 114A 6 7,-1.5 7,-2.3 -2,-0.3 2,-0.3 -0.991 65.2 -67.1 144.7-148.1 -5.0 -5.0 -20.4 13 14 A S E -Ab 18 115A 12 101,-1.4 103,-2.8 -2,-0.3 2,-0.4 -0.996 21.7-157.4-147.0 148.8 -8.2 -7.0 -20.8 14 15 A I E > S-Ab 17 116A 1 3,-2.2 3,-1.6 -2,-0.3 103,-0.2 -0.995 87.4 -11.9-126.3 120.7 -9.6 -10.4 -19.8 15 16 A N T 3 S- 0 0 49 101,-2.5 102,-0.2 -2,-0.4 -1,-0.1 0.873 132.7 -51.8 56.0 39.0 -12.5 -11.8 -21.9 16 17 A G T 3 S+ 0 0 64 100,-0.6 2,-0.5 1,-0.2 -1,-0.3 0.478 113.9 116.6 79.9 3.0 -12.8 -8.4 -23.6 17 18 A H E < -A 14 0A 98 -3,-1.6 -3,-2.2 29,-0.0 -1,-0.2 -0.922 61.3-129.5-112.2 122.3 -13.0 -6.4 -20.3 18 19 A E E +A 13 0A 117 -2,-0.5 2,-0.3 -5,-0.2 -5,-0.3 -0.283 27.6 178.7 -66.5 146.3 -10.3 -3.9 -19.4 19 20 A F E -A 12 0A 7 -7,-2.3 -7,-1.5 26,-0.1 2,-0.3 -0.964 9.1-169.7-144.2 158.8 -8.6 -3.9 -16.1 20 21 A D E +A 11 0A 33 -2,-0.3 21,-2.4 -9,-0.2 22,-0.6 -0.945 5.1 178.5-155.7 129.7 -5.9 -1.8 -14.4 21 22 A M E -AC 10 40A 0 -11,-3.0 -11,-2.6 -2,-0.3 2,-0.3 -0.990 3.2-173.4-135.6 144.2 -3.9 -2.3 -11.2 22 23 A V E +AC 9 39A 63 17,-2.5 17,-2.9 -2,-0.3 2,-0.3 -0.971 29.0 97.1-136.1 150.7 -1.1 -0.3 -9.6 23 24 A G E -AC 8 38A 34 -15,-2.0 -15,-2.6 -2,-0.3 2,-0.3 -0.949 37.3-149.2 169.6-148.7 1.2 -0.8 -6.6 24 25 A G E -AC 7 37A 20 13,-1.7 13,-2.5 -17,-0.3 -17,-0.3 -0.979 8.8-176.6 174.2-166.4 4.6 -2.0 -5.7 25 26 A G E -AC 6 36A 25 -19,-2.7 -19,-2.4 -2,-0.3 2,-0.3 -0.911 22.6-103.2 178.3-151.0 6.7 -3.7 -3.1 26 27 A K E -AC 5 35A 133 9,-1.1 9,-3.0 -21,-0.3 2,-0.3 -0.932 23.9-171.1-155.5 174.8 10.2 -4.8 -2.2 27 28 A G E - C 0 34A 22 -23,-1.6 7,-0.3 -2,-0.3 -2,-0.0 -0.976 29.9-106.8-164.0 175.8 12.4 -7.9 -2.1 28 29 A D E >>> - C 0 33A 72 5,-2.5 4,-1.9 -2,-0.3 3,-1.0 -0.929 20.3-166.7-117.7 103.8 15.7 -9.5 -1.1 29 30 A P T 345S+ 0 0 18 0, 0.0 -1,-0.1 0, 0.0 -25,-0.1 0.544 89.7 56.1 -67.7 -7.6 18.0 -10.2 -4.1 30 31 A N T 345S+ 0 0 129 3,-0.1 32,-0.0 32,-0.1 31,-0.0 0.681 116.8 33.6 -93.9 -23.1 20.2 -12.4 -2.0 31 32 A A T <45S- 0 0 54 -3,-1.0 173,-0.4 2,-0.2 3,-0.1 0.580 101.1-125.1-106.8 -16.0 17.3 -14.7 -0.9 32 33 A G T <5 + 0 0 0 -4,-1.9 2,-0.2 29,-0.6 29,-0.1 0.566 67.7 128.8 80.8 9.1 15.2 -14.6 -4.1 33 34 A S E < +C 28 0A 30 -5,-0.9 -5,-2.5 170,-0.2 2,-0.3 -0.642 27.9 162.8 -99.2 155.4 12.1 -13.4 -2.1 34 35 A L E -CD 27 202A 3 168,-1.8 168,-2.8 -7,-0.3 2,-0.4 -0.985 25.0-143.0-162.8 162.0 9.8 -10.5 -2.7 35 36 A V E -CD 26 201A 55 -9,-3.0 -9,-1.1 -2,-0.3 2,-0.3 -0.981 16.9-178.2-131.8 142.9 6.5 -9.0 -1.9 36 37 A T E -CD 25 200A 1 164,-2.1 164,-3.0 -2,-0.4 2,-0.4 -0.992 8.0-167.5-144.6 135.3 4.2 -7.1 -4.3 37 38 A T E -CD 24 199A 48 -13,-2.5 -13,-1.7 -2,-0.3 2,-0.4 -0.987 8.4-176.1-123.8 132.1 0.8 -5.4 -3.7 38 39 A A E -CD 23 198A 0 160,-2.5 160,-2.6 -2,-0.4 2,-0.4 -0.994 5.5-163.0-130.6 137.2 -1.4 -4.2 -6.5 39 40 A K E -CD 22 197A 115 -17,-2.9 -17,-2.5 -2,-0.4 158,-0.2 -0.956 23.8-113.7-122.8 138.3 -4.7 -2.4 -6.3 40 41 A S E -C 21 0A 14 156,-3.3 -19,-0.3 -2,-0.4 3,-0.1 -0.341 16.7-167.4 -67.5 147.8 -7.4 -1.9 -9.0 41 42 A T S S+ 0 0 77 -21,-2.4 -20,-0.1 1,-0.1 -1,-0.1 0.300 85.5 55.4-114.2 2.5 -8.0 1.5 -10.3 42 43 A K S S- 0 0 117 -22,-0.6 -1,-0.1 1,-0.5 -21,-0.1 -0.075 111.8-102.5-126.8 32.9 -11.3 0.5 -12.1 43 44 A G S S+ 0 0 41 -3,-0.1 -1,-0.5 153,-0.1 -2,-0.1 -0.264 78.8 0.8 78.1-168.4 -13.3 -1.0 -9.2 44 45 A A S S- 0 0 55 1,-0.1 152,-0.2 -3,-0.1 2,-0.1 -0.205 78.0-113.1 -56.1 137.3 -13.8 -4.6 -8.5 45 46 A L - 0 0 10 150,-3.0 -1,-0.1 1,-0.1 -26,-0.1 -0.468 14.7-144.2 -70.3 144.2 -12.1 -7.1 -10.9 46 47 A K S S+ 0 0 123 -2,-0.1 2,-0.3 2,-0.1 -1,-0.1 0.426 81.5 46.8 -90.1 -0.3 -14.6 -9.1 -13.0 47 48 A F S S- 0 0 5 148,-0.1 79,-0.2 80,-0.1 78,-0.1 -0.826 110.3 -58.7-133.0 170.9 -12.4 -12.2 -12.9 48 49 A S > - 0 0 1 77,-2.5 3,-2.1 -2,-0.3 4,-0.2 -0.266 39.1-141.4 -54.4 128.1 -10.5 -14.1 -10.2 49 50 A P G > S+ 0 0 9 0, 0.0 3,-1.8 0, 0.0 -1,-0.2 0.772 100.0 71.4 -62.5 -22.4 -8.0 -11.8 -8.5 50 51 A Y G > S+ 0 0 39 1,-0.3 3,-1.9 2,-0.2 -2,-0.1 0.656 76.4 78.3 -68.1 -13.7 -5.7 -14.9 -8.4 51 52 A L G < S+ 0 0 0 -3,-2.1 -1,-0.3 1,-0.3 4,-0.3 0.713 82.3 67.4 -67.1 -17.3 -5.3 -14.5 -12.2 52 53 A M G <> S+ 0 0 0 -3,-1.8 4,-1.3 -4,-0.2 -1,-0.3 0.504 86.4 122.3 -78.7 -3.6 -2.9 -11.7 -11.3 53 54 A I T <4 S+ 0 0 0 -3,-1.9 138,-0.0 1,-0.3 136,-0.0 -0.893 70.5 12.7-106.0 138.5 -0.6 -14.4 -9.9 54 55 A P T 4 S+ 0 0 19 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 -0.941 113.5 77.8 -87.1 13.4 2.3 -15.1 -10.7 55 56 A H T 4 0 0 3 1,-0.3 -2,-0.2 -4,-0.3 -3,-0.1 0.771 360.0 360.0 -55.0 -30.4 2.5 -11.8 -12.6 56 57 A L < 0 0 2 -4,-1.3 -1,-0.3 -20,-0.1 -3,-0.1 0.702 360.0 360.0 -65.0 360.0 3.0 -9.7 -9.5 57 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 58 61 A Y > 0 0 12 0, 0.0 3,-1.4 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 85.7 10.8 -9.6 -10.9 59 62 A Y G > + 0 0 20 1,-0.3 3,-1.6 2,-0.2 13,-0.2 0.484 360.0 89.0 -76.2 0.9 13.6 -12.1 -11.5 60 63 A Q G 3 S+ 0 0 0 1,-0.3 -1,-0.3 11,-0.1 9,-0.1 0.672 86.0 55.5 -68.6 -13.8 16.0 -9.3 -10.7 61 64 A Y G < S+ 0 0 0 -3,-1.4 -29,-0.6 -29,-0.1 -1,-0.3 0.451 89.1 100.9 -94.1 -2.7 15.6 -10.6 -7.2 62 65 A L < - 0 0 1 -3,-1.6 7,-0.8 -30,-0.1 10,-0.4 -0.740 53.7-165.6 -93.3 113.5 16.6 -14.1 -8.2 63 66 A P E -M 68 0B 35 0, 0.0 143,-0.4 0, 0.0 -33,-0.1 -0.544 18.3-128.1 -89.0 161.9 20.1 -15.3 -7.5 64 67 A Y E > -M 67 0B 18 3,-2.9 3,-1.1 -2,-0.2 5,-0.1 -0.539 31.7 -93.3-101.0 173.2 21.5 -18.5 -9.1 65 68 A P T 3 S+ 0 0 59 0, 0.0 -1,-0.0 0, 0.0 4,-0.0 0.731 122.8 23.8 -58.0 -24.6 23.1 -21.5 -7.5 66 69 A D T 3 S- 0 0 102 1,-0.3 3,-0.0 2,-0.1 0, 0.0 0.125 132.6 -35.6-130.9 22.2 26.6 -20.2 -7.9 67 70 A G E < S-M 64 0B 31 -3,-1.1 -3,-2.9 0, 0.0 -1,-0.3 -0.930 95.7 -17.8 150.5-173.1 26.2 -16.4 -8.2 68 71 A P E -M 63 0B 71 0, 0.0 -2,-0.1 0, 0.0 -38,-0.0 -0.269 66.4-116.7 -61.8 146.1 23.9 -13.8 -9.6 69 72 A S > - 0 0 0 -7,-0.8 4,-2.7 -10,-0.2 3,-0.4 -0.292 28.7-104.2 -76.0 166.7 21.4 -14.8 -12.2 70 73 A P H > S+ 0 0 21 0, 0.0 4,-2.5 0, 0.0 5,-0.2 0.840 124.0 59.1 -61.5 -29.7 21.5 -13.4 -15.8 71 74 A F H > S+ 0 0 0 2,-0.2 4,-1.5 1,-0.2 -11,-0.1 0.879 110.1 41.7 -65.1 -38.4 18.5 -11.3 -14.8 72 75 A Q H > S+ 0 0 3 -3,-0.4 4,-2.8 -10,-0.4 5,-0.2 0.897 112.7 52.3 -76.0 -42.7 20.6 -9.7 -12.0 73 76 A V H X S+ 0 0 48 -4,-2.7 4,-2.3 1,-0.2 6,-0.2 0.923 107.7 54.4 -58.5 -43.5 23.8 -9.4 -14.1 74 77 A S H <>S+ 0 0 5 -4,-2.5 5,-2.7 1,-0.2 -1,-0.2 0.950 111.8 42.8 -54.2 -53.8 21.7 -7.6 -16.7 75 78 A M H ><5S+ 0 0 9 -4,-1.5 3,-1.5 1,-0.2 -1,-0.2 0.897 111.8 54.1 -61.5 -43.1 20.4 -5.0 -14.2 76 79 A L H 3<5S+ 0 0 82 -4,-2.8 -1,-0.2 1,-0.3 -2,-0.2 0.866 108.1 50.0 -60.1 -38.1 23.8 -4.6 -12.5 77 80 A E T 3<5S- 0 0 114 -4,-2.3 -1,-0.3 -5,-0.2 -2,-0.2 0.390 119.5-108.9 -83.5 4.0 25.5 -3.8 -15.9 78 81 A G T < 5S+ 0 0 66 -3,-1.5 -3,-0.2 -4,-0.2 -2,-0.1 0.715 83.5 114.0 77.2 20.2 22.8 -1.2 -16.7 79 82 A S < - 0 0 24 -5,-2.7 2,-0.3 -6,-0.2 -1,-0.2 -0.158 47.6-153.7-103.0-158.8 21.2 -3.3 -19.4 80 83 A G - 0 0 8 -2,-0.1 95,-2.5 -3,-0.1 96,-0.3 -0.978 10.3-147.9-166.9 177.7 17.8 -4.9 -19.5 81 84 A Y E -E 174 0A 10 -2,-0.3 20,-0.4 93,-0.2 2,-0.3 -0.978 12.9-134.7-154.7 159.9 15.4 -7.6 -20.7 82 85 A A E -E 173 0A 16 91,-2.3 91,-2.2 -2,-0.3 2,-0.4 -0.874 16.7-139.7-118.3 153.5 11.7 -8.1 -21.6 83 86 A V E -EF 172 99A 3 16,-2.1 16,-2.7 -2,-0.3 2,-0.4 -0.960 16.6-178.7-120.3 130.5 9.5 -11.0 -20.6 84 87 A Y E +EF 171 98A 76 87,-2.4 87,-2.6 -2,-0.4 2,-0.3 -0.991 8.8 175.3-127.7 127.4 6.9 -12.7 -22.8 85 88 A R E -EF 170 97A 15 12,-1.7 12,-2.6 -2,-0.4 2,-0.4 -0.956 18.9-160.4-132.8 153.4 4.7 -15.5 -21.6 86 89 A V E -EF 169 96A 76 83,-1.8 83,-1.9 -2,-0.3 2,-0.6 -0.995 9.7-160.5-132.0 122.9 1.8 -17.6 -23.0 87 90 A F E -EF 168 95A 0 8,-2.9 8,-1.9 -2,-0.4 2,-0.8 -0.935 0.8-164.7-105.8 120.9 -0.5 -19.4 -20.6 88 91 A D E -EF 167 94A 73 79,-2.8 79,-2.5 -2,-0.6 6,-0.2 -0.884 15.8-155.1-107.9 98.9 -2.5 -22.3 -22.2 89 92 A F E > -E 166 0A 3 4,-2.3 3,-1.8 -2,-0.8 77,-0.3 -0.448 22.1-119.6 -78.0 148.4 -5.2 -23.2 -19.7 90 93 A E T 3 S+ 0 0 110 75,-2.6 -1,-0.1 1,-0.3 76,-0.1 0.724 110.5 51.0 -58.6 -25.2 -6.8 -26.6 -19.6 91 94 A D T 3 S- 0 0 52 74,-0.3 -1,-0.3 2,-0.1 75,-0.1 0.128 127.1 -89.1-102.2 21.7 -10.3 -25.2 -20.3 92 95 A G S < S+ 0 0 36 -3,-1.8 -2,-0.1 1,-0.3 27,-0.1 0.231 80.7 138.4 97.0 -16.2 -9.4 -23.2 -23.4 93 96 A G - 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