==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER OXIDOREDUCTASE 14-MAR-07 2EIX . COMPND 2 MOLECULE: NADH-CYTOCHROME B5 REDUCTASE; . SOURCE 2 ORGANISM_SCIENTIFIC: PHYSARUM POLYCEPHALUM; . AUTHOR S.W.KIM,M.SUGA,K.OGASAHARA,T.IKEGAMI,Y.MINAMI,T.YUBISUI,T.TS . 486 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 24178.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 318 65.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 64 13.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 72 14.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 36 7.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 49 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 81 16.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 4 0 0 2 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 2 2 0 0 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 0 4 0 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 2 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 39 A K 0 0 263 0, 0.0 2,-0.2 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 110.6 22.1 21.7 1.1 2 40 A R - 0 0 122 1,-0.1 3,-0.1 33,-0.0 33,-0.0 -0.485 360.0-110.2 -90.0 155.7 19.0 20.4 -0.8 3 41 A E - 0 0 142 -2,-0.2 -1,-0.1 1,-0.1 4,-0.1 -0.410 50.2 -83.3 -71.4 159.7 16.5 17.7 0.1 4 42 A P - 0 0 95 0, 0.0 -1,-0.1 0, 0.0 36,-0.1 -0.194 41.3-113.7 -56.1 156.5 16.6 14.4 -1.8 5 43 A A S S+ 0 0 10 34,-0.6 2,-0.3 -3,-0.1 35,-0.1 0.907 98.2 41.8 -65.4 -37.6 14.6 14.5 -5.1 6 44 A L - 0 0 12 34,-0.4 97,-0.1 35,-0.1 5,-0.1 -0.827 65.4-157.2-110.7 148.1 12.0 12.0 -3.9 7 45 A N - 0 0 48 3,-0.6 97,-1.8 -2,-0.3 3,-0.1 -0.968 15.8-142.4-117.3 133.3 10.2 11.7 -0.5 8 46 A P S S+ 0 0 51 0, 0.0 -1,-0.1 0, 0.0 95,-0.1 0.774 98.7 39.2 -69.6 -19.9 8.8 8.2 0.3 9 47 A N S S+ 0 0 120 1,-0.2 2,-0.4 95,-0.1 94,-0.1 0.649 112.5 49.7-102.5 -21.2 5.7 9.7 2.0 10 48 A E S S- 0 0 136 94,-0.2 -3,-0.6 -3,-0.1 94,-0.3 -0.984 73.2-127.1-131.1 133.1 4.8 12.7 -0.1 11 49 A Y - 0 0 45 -2,-0.4 2,-0.3 92,-0.1 92,-0.2 -0.455 25.7-168.0 -69.8 140.9 4.2 13.2 -3.8 12 50 A K E -A 102 0A 47 90,-1.8 90,-2.8 -2,-0.1 2,-0.4 -0.928 23.1-109.9-124.1 156.9 6.1 16.0 -5.5 13 51 A K E -A 101 0A 116 -2,-0.3 2,-0.4 88,-0.2 88,-0.2 -0.719 27.7-171.0 -93.0 134.5 5.6 17.5 -9.0 14 52 A F E -A 100 0A 1 86,-2.4 86,-1.6 -2,-0.4 2,-0.3 -0.990 21.5-125.2-127.6 128.1 8.0 16.9 -11.8 15 53 A M E -A 99 0A 69 -2,-0.4 17,-3.1 17,-0.4 2,-0.5 -0.504 16.3-122.6 -78.1 132.8 7.7 18.8 -15.1 16 54 A L E -D 31 0B 1 82,-3.2 81,-2.5 79,-0.3 15,-0.2 -0.608 28.2-178.0 -67.1 118.2 7.6 17.0 -18.5 17 55 A R E - 0 0 101 13,-3.3 2,-0.3 -2,-0.5 14,-0.2 0.906 58.4 -38.4 -84.4 -50.5 10.6 18.4 -20.5 18 56 A E E -D 30 0B 93 12,-1.1 12,-2.8 78,-0.1 -1,-0.4 -0.969 51.2-140.6-165.5 169.0 10.0 16.5 -23.8 19 57 A K E -D 29 0B 84 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.965 8.7-162.2-142.4 154.3 9.0 13.2 -25.3 20 58 A Q E -D 28 0B 115 8,-2.2 8,-2.8 -2,-0.3 2,-0.2 -1.000 24.1-117.7-137.4 141.0 10.0 11.1 -28.1 21 59 A I E +D 27 0B 124 -2,-0.3 6,-0.2 6,-0.2 3,-0.1 -0.573 27.1 176.8 -71.9 133.4 8.4 8.2 -30.0 22 60 A I E - 0 0 69 4,-2.7 2,-0.2 1,-0.5 5,-0.2 0.753 66.8 -29.0-105.3 -46.1 10.5 4.9 -29.7 23 61 A N E > S-D 26 0B 21 3,-1.5 3,-0.6 136,-0.0 -1,-0.5 -0.837 89.6 -59.5-154.8-171.1 8.2 2.6 -31.7 24 62 A H T 3 S+ 0 0 119 -2,-0.2 3,-0.0 1,-0.2 -3,-0.0 0.821 133.5 13.7 -52.9 -31.6 4.4 2.2 -32.5 25 63 A N T 3 S+ 0 0 39 55,-0.0 56,-3.2 -4,-0.0 2,-0.4 0.306 112.4 81.1-130.3 18.9 3.4 2.0 -28.8 26 64 A T E < +DE 23 80B 1 -3,-0.6 -4,-2.7 54,-0.2 -3,-1.5 -0.950 48.2 178.6-128.0 137.4 6.4 3.0 -26.8 27 65 A R E -DE 21 79B 43 52,-2.4 52,-2.8 -2,-0.4 2,-0.5 -0.950 23.5-134.3-130.6 156.2 7.6 6.6 -25.9 28 66 A L E -DE 20 78B 27 -8,-2.8 -8,-2.2 -2,-0.3 2,-0.5 -0.960 22.6-163.7-110.4 129.3 10.5 8.0 -23.9 29 67 A F E -DE 19 77B 0 48,-3.3 48,-3.0 -2,-0.5 2,-0.6 -0.951 6.1-154.7-118.1 130.7 9.5 10.8 -21.5 30 68 A R E -DE 18 76B 62 -12,-2.8 -13,-3.3 -2,-0.5 -12,-1.1 -0.901 12.4-168.2-108.7 120.6 12.1 13.1 -19.9 31 69 A F E -DE 16 75B 0 44,-3.0 44,-2.1 -2,-0.6 2,-0.3 -0.871 19.3-122.2-109.2 137.3 11.1 14.8 -16.6 32 70 A N E - E 0 74B 40 -17,-3.1 -17,-0.4 -2,-0.4 42,-0.3 -0.534 14.2-142.2 -75.0 137.4 12.9 17.7 -14.9 33 71 A L - 0 0 9 40,-2.0 7,-0.0 -2,-0.3 -18,-0.0 -0.508 34.0-105.4 -83.8 168.0 14.3 17.3 -11.4 34 72 A H S S+ 0 0 121 -2,-0.2 -1,-0.1 1,-0.1 -2,-0.0 0.706 103.2 32.7 -76.7 -20.6 13.9 20.6 -9.4 35 73 A H S > S- 0 0 63 1,-0.0 3,-1.8 -33,-0.0 38,-0.3 -0.991 80.2-125.7-133.9 140.8 17.6 21.6 -9.6 36 74 A P T 3 S+ 0 0 99 0, 0.0 38,-0.1 0, 0.0 -2,-0.0 0.641 110.8 51.0 -57.8 -17.6 20.0 20.9 -12.4 37 75 A E T 3 S+ 0 0 126 35,-0.1 2,-0.1 36,-0.1 -3,-0.0 0.366 88.0 107.7-102.3 1.7 22.5 19.2 -10.1 38 76 A D < - 0 0 18 -3,-1.8 35,-3.1 34,-0.1 2,-0.3 -0.417 54.4-153.9 -70.2 152.8 19.9 16.8 -8.5 39 77 A V - 0 0 60 33,-0.2 -34,-0.6 34,-0.1 33,-0.1 -0.925 29.3-115.0-120.0 157.5 19.9 13.1 -9.3 40 78 A V - 0 0 11 31,-0.4 -34,-0.4 34,-0.4 -1,-0.1 0.880 41.3-152.2 -63.0 -38.4 16.7 11.0 -9.0 41 79 A G + 0 0 49 28,-0.4 -1,-0.1 1,-0.2 -35,-0.1 0.835 29.1 162.3 79.3 32.8 18.3 9.0 -6.1 42 80 A L - 0 0 10 4,-0.1 -1,-0.2 27,-0.1 3,-0.1 -0.757 36.4-127.4 -87.4 124.4 16.7 5.6 -6.3 43 81 A P > - 0 0 58 0, 0.0 3,-2.3 0, 0.0 66,-0.1 -0.225 44.8 -74.2 -61.9 164.4 18.6 2.9 -4.4 44 82 A I T 3 S+ 0 0 37 1,-0.3 67,-0.4 67,-0.1 90,-0.1 -0.264 121.2 15.2 -66.5 125.2 19.4 -0.3 -6.3 45 83 A G T 3 S+ 0 0 4 1,-0.3 64,-0.4 85,-0.2 -1,-0.3 0.386 104.1 109.8 97.5 -1.1 16.3 -2.4 -6.8 46 84 A Q < - 0 0 38 -3,-2.3 -1,-0.3 61,-0.1 2,-0.3 -0.563 45.9-159.1-116.7 165.0 13.8 0.3 -5.9 47 85 A H E -B 106 0A 35 59,-2.6 59,-2.4 -2,-0.2 2,-0.3 -0.794 18.3-106.5-133.5 171.7 11.2 2.3 -7.9 48 86 A M E - C 0 63A 0 15,-2.6 15,-2.0 -2,-0.3 2,-0.5 -0.661 23.8-138.5-102.9 158.7 9.1 5.4 -8.0 49 87 A S E -BC 103 62A 18 54,-2.8 54,-2.6 -2,-0.3 2,-0.4 -0.984 17.8-162.0-118.3 124.5 5.4 5.5 -7.4 50 88 A V E -BC 102 61A 0 11,-2.6 11,-2.2 -2,-0.5 2,-0.3 -0.890 3.4-165.7-110.8 144.4 3.3 7.8 -9.7 51 89 A K E +BC 101 60A 32 50,-2.2 50,-0.8 -2,-0.4 2,-0.3 -0.930 9.8 170.6-124.2 144.4 -0.2 9.1 -9.1 52 90 A A E - C 0 59A 0 7,-2.2 7,-3.0 -2,-0.3 2,-0.6 -0.980 32.2-119.3-144.6 168.6 -2.7 10.7 -11.3 53 91 A T E + C 0 58A 71 -2,-0.3 2,-0.4 5,-0.2 5,-0.2 -0.915 32.2 173.0-108.0 120.3 -6.3 11.7 -11.3 54 92 A V E > S- C 0 57A 35 3,-2.7 3,-1.9 -2,-0.6 -2,-0.1 -0.993 71.8 -15.7-128.1 121.6 -8.5 10.0 -13.9 55 93 A D T 3 S- 0 0 134 -2,-0.4 -1,-0.1 1,-0.3 3,-0.1 0.874 129.3 -50.6 50.7 42.8 -12.3 10.6 -13.9 56 94 A G T 3 S+ 0 0 71 1,-0.2 2,-0.3 -3,-0.0 -1,-0.3 0.417 119.8 97.7 85.5 -1.0 -12.2 12.0 -10.3 57 95 A K E < S-C 54 0A 43 -3,-1.9 -3,-2.7 0, 0.0 2,-0.3 -0.931 71.4-119.1-126.1 149.6 -10.3 9.2 -8.8 58 96 A E E -C 53 0A 71 418,-0.4 2,-0.3 -2,-0.3 -5,-0.2 -0.662 27.8-168.9 -83.5 130.4 -6.6 8.7 -8.0 59 97 A I E -C 52 0A 5 -7,-3.0 -7,-2.2 -2,-0.3 2,-0.4 -0.883 3.6-156.4-115.9 156.1 -4.7 5.9 -9.7 60 98 A Y E +C 51 0A 58 -2,-0.3 -9,-0.2 -9,-0.2 416,-0.1 -0.999 15.2 166.2-137.8 128.5 -1.2 4.6 -8.8 61 99 A R E -C 50 0A 69 -11,-2.2 -11,-2.6 -2,-0.4 2,-0.2 -0.998 28.8-120.8-140.3 148.7 1.5 2.8 -10.7 62 100 A P E +C 49 0A 82 0, 0.0 2,-0.3 0, 0.0 -13,-0.2 -0.598 28.4 177.2 -84.3 147.5 5.2 2.0 -10.3 63 101 A Y E -C 48 0A 45 -15,-2.0 -15,-2.6 -2,-0.2 16,-0.0 -0.993 19.9-150.8-143.3 152.4 7.9 2.9 -12.7 64 102 A T - 0 0 14 -2,-0.3 14,-0.3 -17,-0.2 -17,-0.1 -0.973 27.6-124.9-123.5 120.0 11.7 2.6 -12.9 65 103 A P - 0 0 10 0, 0.0 12,-0.2 0, 0.0 68,-0.1 -0.226 7.7-151.9 -61.1 149.3 13.6 5.3 -14.8 66 104 A V + 0 0 15 10,-2.5 11,-0.1 2,-0.1 2,-0.1 0.451 67.8 101.9 -99.4 -2.9 16.0 4.1 -17.6 67 105 A S S S- 0 0 13 9,-0.9 2,-0.0 1,-0.1 8,-0.0 -0.500 87.5 -99.8 -71.3 147.2 18.3 7.1 -17.2 68 106 A S > - 0 0 32 -2,-0.1 3,-2.0 1,-0.1 -1,-0.1 -0.314 32.4-104.0 -71.7 160.1 21.4 6.0 -15.3 69 107 A D T 3 S+ 0 0 78 1,-0.3 -28,-0.4 64,-0.1 -1,-0.1 0.714 116.8 51.8 -57.1 -25.4 21.7 6.7 -11.6 70 108 A D T 3 S+ 0 0 110 -31,-0.1 -1,-0.3 -30,-0.1 2,-0.2 0.396 73.3 124.1 -97.2 3.0 24.1 9.6 -12.2 71 109 A E < - 0 0 69 -3,-2.0 -31,-0.4 1,-0.1 2,-0.4 -0.501 56.5-134.0 -66.5 134.8 22.3 11.7 -14.8 72 110 A K + 0 0 125 -2,-0.2 -33,-0.2 1,-0.2 -35,-0.1 -0.770 68.6 2.6-100.9 129.6 21.9 15.2 -13.5 73 111 A G S S+ 0 0 5 -35,-3.1 -40,-2.0 -2,-0.4 2,-0.3 0.447 118.4 39.1 90.3 -2.6 18.7 17.4 -13.5 74 112 A Y E -E 32 0B 28 -36,-0.3 -34,-0.4 -42,-0.3 2,-0.3 -0.974 59.4-142.0-157.9 169.2 16.3 14.9 -15.0 75 113 A F E -E 31 0B 0 -44,-2.1 -44,-3.0 -2,-0.3 2,-0.4 -0.972 17.8-139.6-131.4 158.8 15.0 11.4 -15.2 76 114 A D E -E 30 0B 14 -2,-0.3 -10,-2.5 -46,-0.2 -9,-0.9 -0.939 10.7-166.6-119.4 134.0 13.8 9.4 -18.2 77 115 A L E -E 29 0B 2 -48,-3.0 -48,-3.3 -2,-0.4 2,-0.5 -0.976 8.9-155.9-114.5 130.6 10.8 6.9 -18.4 78 116 A I E -E 28 0B 15 -2,-0.4 2,-0.4 -14,-0.3 -50,-0.2 -0.931 24.6-173.5 -99.9 124.9 10.3 4.6 -21.3 79 117 A I E -E 27 0B 4 -52,-2.8 -52,-2.4 -2,-0.5 2,-0.5 -0.969 23.3-144.7-131.4 127.3 6.6 3.8 -21.2 80 118 A K E -E 26 0B 33 -2,-0.4 2,-0.6 -54,-0.2 -54,-0.2 -0.769 24.6-138.9 -84.5 131.3 4.5 1.3 -23.3 81 119 A V - 0 0 20 -56,-3.2 2,-0.3 -2,-0.5 3,-0.0 -0.814 15.5-157.4-100.0 122.8 1.1 2.8 -24.0 82 120 A Y > - 0 0 114 -2,-0.6 3,-1.5 3,-0.1 7,-0.1 -0.747 23.0-122.8 -99.8 146.2 -1.8 0.4 -23.7 83 121 A E T 3 S+ 0 0 184 -2,-0.3 -1,-0.1 1,-0.3 -2,-0.0 0.860 112.8 18.3 -55.2 -40.5 -5.2 0.9 -25.4 84 122 A K T 3 S+ 0 0 169 -3,-0.0 2,-0.7 5,-0.0 -1,-0.3 -0.131 93.0 125.7-122.8 35.3 -7.2 0.7 -22.2 85 123 A G <> - 0 0 20 -3,-1.5 4,-2.1 1,-0.2 5,-0.2 -0.866 45.9-160.4 -96.2 110.8 -4.3 1.4 -19.8 86 124 A Q H > S+ 0 0 76 -2,-0.7 4,-2.2 1,-0.2 -1,-0.2 0.895 84.9 39.7 -61.6 -48.1 -5.3 4.4 -17.7 87 125 A M H > S+ 0 0 2 2,-0.2 4,-3.0 3,-0.2 -1,-0.2 0.922 115.7 48.2 -70.9 -42.5 -1.9 5.5 -16.5 88 126 A S H > S+ 0 0 8 2,-0.2 4,-2.7 1,-0.2 -2,-0.2 0.903 114.7 49.4 -67.0 -36.7 0.1 5.0 -19.7 89 127 A Q H X S+ 0 0 56 -4,-2.1 4,-1.1 2,-0.2 -1,-0.2 0.934 111.6 48.0 -62.6 -47.6 -2.7 6.9 -21.5 90 128 A Y H >< S+ 0 0 76 -4,-2.2 3,-0.9 -5,-0.2 4,-0.3 0.943 113.4 46.6 -54.4 -53.8 -2.6 9.7 -19.0 91 129 A I H >< S+ 0 0 0 -4,-3.0 3,-1.7 1,-0.2 -2,-0.2 0.912 109.7 54.7 -58.8 -43.2 1.1 10.0 -19.2 92 130 A D H 3< S+ 0 0 31 -4,-2.7 -1,-0.2 1,-0.3 -2,-0.2 0.672 105.5 54.9 -69.0 -13.1 1.0 9.9 -23.1 93 131 A H T << S+ 0 0 131 -4,-1.1 -1,-0.3 -3,-0.9 2,-0.2 0.416 82.1 110.9 -98.7 -0.3 -1.5 12.9 -23.1 94 132 A L < - 0 0 12 -3,-1.7 3,-0.0 -4,-0.3 6,-0.0 -0.529 58.2-147.3 -70.8 141.9 0.8 15.1 -21.0 95 133 A N > - 0 0 106 -2,-0.2 3,-2.3 1,-0.1 -79,-0.3 -0.810 36.5 -83.9 -98.4 152.5 2.2 18.1 -22.9 96 134 A P T 3 S+ 0 0 83 0, 0.0 -79,-0.2 0, 0.0 3,-0.1 -0.282 121.6 38.4 -51.1 135.8 5.8 19.6 -22.1 97 135 A G T 3 S+ 0 0 37 -81,-2.5 2,-0.1 1,-0.5 -80,-0.1 0.142 92.7 110.8 99.8 -15.6 5.3 21.9 -19.1 98 136 A D < - 0 0 61 -3,-2.3 -82,-3.2 -82,-0.1 -1,-0.5 -0.417 63.9-123.2 -83.7 160.6 2.7 19.6 -17.4 99 137 A F E -A 15 0A 80 -84,-0.3 2,-0.4 -2,-0.1 -84,-0.2 -0.723 12.0-155.4-109.1 160.3 3.6 17.8 -14.1 100 138 A L E -A 14 0A 1 -86,-1.6 -86,-2.4 -2,-0.2 2,-0.6 -0.981 23.0-130.1-130.4 135.1 3.6 14.3 -13.1 101 139 A Q E +AB 13 51A 53 -50,-0.8 -50,-2.2 -2,-0.4 2,-0.4 -0.831 34.6 174.0 -95.8 126.9 3.3 13.5 -9.3 102 140 A V E +AB 12 50A 1 -90,-2.8 -90,-1.8 -2,-0.6 2,-0.3 -0.972 15.5 179.4-135.3 140.4 5.9 11.1 -8.0 103 141 A R E - B 0 49A 74 -54,-2.6 -54,-2.8 -2,-0.4 -92,-0.1 -0.915 30.0 -25.2-132.6 171.4 7.0 9.7 -4.6 104 142 A G E - 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