==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=18-JAN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 12-APR-12 4EMQ . COMPND 2 MOLECULE: FLUORESCENT PROTEIN DRONPA; . SOURCE 2 ORGANISM_SCIENTIFIC: ECHINOPHYLLIA SP. SC22; . AUTHOR N.B.NGAN,K.VAN HECKE,L.VAN MEERVELT . 1280 12 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 51497.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 939 73.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 77 6.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 614 48.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 18 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 98 7.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 91 7.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 44 3.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 6 0 0 0 0 0 0 0 1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 6 0 10 2 6 6 12 12 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 134 0, 0.0 3,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-129.6 35.9 26.9 15.4 2 3 A V + 0 0 41 75,-0.1 2,-0.5 78,-0.1 75,-0.0 0.598 360.0 82.0 -90.9 -19.8 32.9 25.5 13.6 3 4 A I - 0 0 12 77,-0.1 4,-0.1 74,-0.1 106,-0.1 -0.753 64.7-172.1 -87.3 124.7 33.9 28.0 10.8 4 5 A K - 0 0 104 -2,-0.5 29,-0.2 105,-0.1 3,-0.1 -0.764 37.9-111.2-108.9 159.9 36.7 26.9 8.4 5 6 A P S S+ 0 0 85 0, 0.0 2,-0.5 0, 0.0 28,-0.3 0.817 112.4 40.9 -59.5 -31.2 38.5 28.9 5.7 6 7 A D S S+ 0 0 92 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.981 77.4 173.8-118.9 121.5 36.8 26.7 3.2 7 8 A M E -A 30 0A 0 23,-2.8 23,-2.8 -2,-0.5 2,-0.3 -0.861 21.4-130.6-124.3 160.2 33.2 25.8 3.7 8 9 A K E -Ab 29 110A 96 101,-3.2 103,-2.8 -2,-0.3 2,-0.4 -0.728 13.9-152.7-108.9 160.9 30.6 24.0 1.6 9 10 A I E +Ab 28 111A 0 19,-2.1 19,-2.7 -2,-0.3 2,-0.4 -0.998 13.9 174.8-136.0 134.3 27.0 24.9 0.7 10 11 A K E +Ab 27 112A 115 101,-2.2 103,-2.6 -2,-0.4 2,-0.3 -0.994 17.1 171.0-132.6 140.1 23.9 22.8 -0.2 11 12 A L E -Ab 26 113A 10 15,-2.3 15,-1.9 -2,-0.4 2,-0.4 -0.993 25.2-162.9-147.1 152.0 20.4 24.3 -0.7 12 13 A R E -Ab 25 114A 68 101,-1.7 103,-2.6 -2,-0.3 2,-0.5 -0.994 12.1-167.2-130.2 131.7 16.9 23.8 -1.9 13 14 A M E -Ab 24 115A 0 11,-3.0 11,-2.4 -2,-0.4 2,-0.4 -0.978 2.9-173.6-112.6 128.1 14.6 26.6 -2.8 14 15 A E E +Ab 23 116A 90 101,-2.4 103,-1.8 -2,-0.5 2,-0.3 -0.959 28.8 124.9-114.4 141.1 10.9 26.0 -3.2 15 16 A G E -Ab 22 117A 3 7,-2.4 7,-2.2 -2,-0.4 2,-0.3 -0.940 42.7-125.4-170.0-173.3 8.8 28.8 -4.5 16 17 A A E -Ab 21 118A 10 101,-1.8 103,-2.4 -2,-0.3 2,-0.4 -0.980 5.5-158.2-153.9 150.6 6.3 30.5 -6.8 17 18 A V E > S-Ab 20 119A 4 3,-1.6 3,-2.4 -2,-0.3 103,-0.2 -0.996 86.0 -12.9-131.4 126.3 6.1 33.6 -8.9 18 19 A N T 3 S- 0 0 9 101,-2.5 102,-0.2 -2,-0.4 -1,-0.1 0.805 132.1 -53.0 51.8 31.9 2.7 34.9 -9.9 19 20 A G T 3 S+ 0 0 34 100,-0.6 -1,-0.3 1,-0.2 101,-0.1 0.272 111.0 120.2 93.5 -10.8 1.2 31.6 -8.7 20 21 A H E < -A 17 0A 126 -3,-2.4 -3,-1.6 1,-0.0 -1,-0.2 -0.787 56.2-139.9 -98.1 115.3 3.5 29.4 -10.8 21 22 A P E +A 16 0A 86 0, 0.0 -5,-0.3 0, 0.0 2,-0.3 -0.206 28.6 159.8 -76.7 160.5 5.5 27.0 -8.6 22 23 A F E -A 15 0A 5 -7,-2.2 -7,-2.4 24,-0.1 2,-0.3 -0.950 20.2-151.5-166.0 173.4 9.2 26.0 -9.1 23 24 A A E -AC 14 45A 0 22,-2.3 21,-2.5 -2,-0.3 22,-1.6 -0.977 6.6-170.6-160.3 142.6 12.2 24.6 -7.2 24 25 A I E -AC 13 43A 0 -11,-2.4 -11,-3.0 -2,-0.3 2,-0.3 -0.994 3.3-163.8-140.4 143.4 15.9 24.9 -7.5 25 26 A E E +AC 12 42A 73 17,-2.1 17,-2.7 -2,-0.3 2,-0.3 -0.937 12.7 176.0-126.4 152.9 18.8 23.0 -5.8 26 27 A G E -AC 11 41A 8 -15,-1.9 -15,-2.3 -2,-0.3 2,-0.3 -0.984 19.9-158.0-153.2 162.1 22.4 23.9 -5.6 27 28 A V E +AC 10 40A 90 13,-1.6 13,-2.7 -2,-0.3 2,-0.3 -0.983 29.6 151.3-140.4 142.2 25.8 23.0 -4.2 28 29 A G E -AC 9 39A 10 -19,-2.7 -19,-2.1 -2,-0.3 2,-0.3 -0.872 35.6-117.6-157.1-172.6 28.6 25.4 -3.7 29 30 A L E -AC 8 38A 78 9,-2.7 9,-2.6 -2,-0.3 2,-0.3 -0.997 25.7-176.6-141.9 141.9 31.8 26.4 -1.9 30 31 A G E -AC 7 37A 2 -23,-2.8 -23,-2.8 -2,-0.3 7,-0.3 -0.911 25.2-142.2-137.9 164.5 32.5 29.6 0.1 31 32 A K E >>> - C 0 36A 86 5,-3.3 4,-1.7 -2,-0.3 5,-1.4 -0.861 15.7-167.2-128.5 94.9 35.3 31.3 1.9 32 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.5 0, 0.0 -1,-0.1 0.760 86.7 44.3 -56.9 -32.8 33.8 33.0 5.0 33 34 A F T 345S+ 0 0 93 -28,-0.3 -27,-0.1 1,-0.2 32,-0.1 0.700 116.4 45.6 -90.8 -20.2 36.8 35.1 5.9 34 35 A E T <45S- 0 0 106 -3,-0.8 33,-0.3 -29,-0.3 -1,-0.2 0.599 104.8-131.1 -89.7 -17.1 37.5 36.1 2.3 35 36 A G T <5 + 0 0 0 -4,-1.7 175,-2.2 31,-0.3 2,-0.3 0.927 63.7 126.4 65.9 45.7 33.8 36.9 1.8 36 37 A K E < +CD 31 209A 112 -5,-1.4 -5,-3.3 173,-0.2 2,-0.3 -0.981 28.9 164.6-135.0 144.2 33.6 34.9 -1.5 37 38 A Q E -CD 30 208A 12 171,-2.2 171,-1.9 -2,-0.3 2,-0.3 -0.995 12.4-171.7-155.9 155.2 31.4 32.1 -2.6 38 39 A S E +CD 29 207A 41 -9,-2.6 -9,-2.7 -2,-0.3 2,-0.3 -0.992 9.2 178.7-146.9 150.2 30.3 30.3 -5.7 39 40 A M E -CD 28 206A 2 167,-2.3 167,-2.5 -2,-0.3 2,-0.5 -0.989 25.8-130.9-149.6 158.2 27.6 27.7 -6.5 40 41 A D E -CD 27 205A 106 -13,-2.7 -13,-1.6 -2,-0.3 2,-0.3 -0.955 30.1-164.0-112.7 129.3 26.1 25.6 -9.3 41 42 A L E -CD 26 204A 1 163,-3.6 163,-2.3 -2,-0.5 2,-0.4 -0.878 9.6-167.8-119.4 144.6 22.3 25.7 -9.5 42 43 A K E -CD 25 203A 118 -17,-2.7 -17,-2.1 -2,-0.3 2,-0.5 -0.988 23.4-129.2-134.1 130.6 19.8 23.5 -11.2 43 44 A V E +C 24 0A 26 159,-1.8 -19,-0.3 -2,-0.4 3,-0.1 -0.634 29.0 174.7 -76.1 120.7 16.1 24.1 -11.8 44 45 A K E + 0 0 107 -21,-2.5 2,-0.3 -2,-0.5 -20,-0.2 0.681 64.2 15.7-102.6 -25.1 14.3 21.0 -10.6 45 46 A E E S+C 23 0A 109 -22,-1.6 -22,-2.3 1,-0.0 -1,-0.3 -0.983 116.7 28.2-148.2 147.6 10.7 22.0 -11.0 46 47 A G S S- 0 0 43 -2,-0.3 -24,-0.1 -24,-0.3 -22,-0.1 0.262 89.9-178.8 89.8 -10.7 8.8 24.8 -12.8 47 48 A G + 0 0 42 -24,-0.2 -1,-0.3 2,-0.1 2,-0.1 -0.338 54.6 52.1 -79.9 158.5 11.5 25.2 -15.6 48 49 A P S S- 0 0 79 0, 0.0 153,-0.1 0, 0.0 -2,-0.0 0.439 96.4-127.4 -71.7 130.8 12.3 26.7 -18.0 49 50 A L - 0 0 5 151,-0.5 -2,-0.1 1,-0.2 4,-0.1 -0.298 13.5-152.6 -59.1 128.6 11.6 29.6 -15.6 50 51 A P S S+ 0 0 60 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.205 74.8 60.3 -86.5 14.9 9.2 32.1 -17.2 51 52 A F S S- 0 0 7 79,-0.1 2,-0.2 80,-0.1 78,-0.2 -0.910 103.3 -73.5-136.1 166.1 10.7 35.0 -15.2 52 53 A A > - 0 0 4 76,-1.9 3,-1.6 -2,-0.3 4,-0.3 -0.399 33.3-145.4 -64.3 125.4 14.1 36.6 -14.9 53 54 A Y T >> S+ 0 0 7 1,-0.3 3,-3.0 -2,-0.2 4,-0.8 0.846 91.1 77.6 -59.5 -34.4 16.4 34.3 -12.9 54 55 A D H 3> S+ 0 0 9 1,-0.3 4,-0.5 2,-0.2 3,-0.4 0.761 79.6 66.9 -48.1 -30.6 18.0 37.5 -11.5 55 56 A I H <4 S+ 0 0 0 -3,-1.6 -1,-0.3 1,-0.2 -2,-0.2 0.728 105.0 46.4 -67.0 -17.6 15.2 38.0 -9.0 56 57 A L H X> S+ 0 0 0 -3,-3.0 3,-2.2 -4,-0.3 4,-1.1 0.751 88.6 84.4 -89.2 -29.0 16.3 34.7 -7.3 57 58 A T H 3< S+ 0 0 1 -4,-0.8 3,-0.2 -3,-0.4 -2,-0.1 0.775 85.0 52.2 -60.9 -35.5 20.0 35.2 -7.0 58 59 A T T 3< S+ 0 0 25 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.600 104.8 59.9 -76.4 -5.9 20.4 37.2 -3.8 59 60 A V T <4 0 0 0 -3,-2.2 -1,-0.2 1,-0.1 -2,-0.2 0.832 360.0 360.0 -85.8 -33.5 18.3 34.6 -2.1 60 61 A F < 0 0 9 -4,-1.1 -2,-0.2 -3,-0.2 -3,-0.1 0.842 360.0 360.0 -71.5 360.0 20.8 31.9 -2.9 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 65 A N > 0 0 5 0, 0.0 3,-2.2 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 105.0 24.2 33.9 3.6 63 66 A R T 3 + 0 0 12 1,-0.3 3,-0.4 2,-0.1 14,-0.2 0.524 360.0 85.5 -76.1 -1.5 26.0 36.9 5.2 64 67 A V T 3 S+ 0 0 0 1,-0.3 2,-1.7 12,-0.2 -1,-0.3 0.869 80.5 67.8 -53.2 -37.4 28.4 34.4 6.8 65 68 A F S < S+ 0 0 1 -3,-2.2 2,-0.4 -30,-0.1 -1,-0.3 -0.373 79.2 104.7 -87.2 61.4 30.2 34.8 3.4 66 69 A A S S- 0 0 0 -2,-1.7 2,-0.8 -34,-0.5 -31,-0.3 -0.997 75.4-120.0-137.3 134.8 31.2 38.4 4.1 67 70 A K B -e 211 0A 99 143,-2.5 145,-2.5 -2,-0.4 -34,-0.1 -0.685 34.1-167.0 -77.0 109.3 34.7 39.6 5.0 68 71 A Y - 0 0 31 -2,-0.8 145,-0.1 143,-0.2 6,-0.1 -0.854 14.9-133.5 -99.7 130.1 34.4 41.3 8.4 69 72 A P > - 0 0 48 0, 0.0 3,-1.7 0, 0.0 144,-0.0 -0.392 30.9-106.1 -73.1 160.0 37.2 43.4 9.9 70 73 A E T 3 S+ 0 0 114 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.758 115.3 60.3 -58.4 -29.0 38.2 42.7 13.5 71 74 A N T 3 S+ 0 0 129 2,-0.1 2,-0.4 143,-0.0 -1,-0.3 0.200 92.5 75.1 -91.0 14.3 36.6 45.9 14.8 72 75 A I S < S- 0 0 15 -3,-1.7 2,-0.2 111,-0.1 140,-0.1 -0.998 86.5-116.6-126.0 131.3 33.1 45.1 13.6 73 76 A V - 0 0 27 -2,-0.4 2,-1.0 109,-0.1 3,-0.1 -0.433 22.3-144.0 -64.6 130.0 30.9 42.6 15.4 74 77 A D > + 0 0 22 -2,-0.2 4,-1.7 1,-0.2 107,-0.1 -0.794 19.6 179.9-102.4 93.5 30.1 39.6 13.2 75 78 A Y T 4 S+ 0 0 6 -2,-1.0 4,-0.4 1,-0.2 -1,-0.2 0.838 78.5 50.3 -57.5 -34.6 26.5 38.6 14.1 76 79 A F T >4 S+ 0 0 0 2,-0.2 3,-0.9 1,-0.2 4,-0.3 0.942 109.7 42.1 -81.7 -52.7 26.6 35.8 11.5 77 80 A K G >4 S+ 0 0 25 1,-0.2 3,-1.5 -14,-0.2 -1,-0.2 0.906 112.5 57.7 -60.1 -39.4 29.8 33.8 12.2 78 81 A Q G 3< S+ 0 0 73 -4,-1.7 -1,-0.2 1,-0.2 -2,-0.2 0.725 94.9 68.2 -60.9 -20.6 29.0 34.1 16.0 79 82 A S G <> S+ 0 0 0 -3,-0.9 4,-2.1 -4,-0.4 98,-0.3 0.595 86.3 131.1 -83.7 -6.4 25.7 32.4 15.5 80 83 A F T <4 + 0 0 8 -3,-1.5 -78,-0.1 -4,-0.3 -77,-0.1 -0.612 48.6 29.8 -95.6 156.7 27.1 29.0 14.6 81 84 A P T 4 S+ 0 0 58 0, 0.0 25,-0.4 0, 0.0 -1,-0.3 -0.980 131.8 41.8 -76.3 -15.1 27.0 26.1 15.1 82 85 A E T 4 S- 0 0 89 -3,-0.2 -2,-0.3 1,-0.2 2,-0.1 0.821 100.1-161.5 -64.8 -31.9 23.3 26.7 15.9 83 86 A G < - 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