==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=30-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSFERASE 25-OCT-05 2ERZ . COMPND 2 MOLECULE: CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR M.JACOBS . 356 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 16341.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 227 63.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 2 0.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 45 12.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 28 7.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 40 11.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 97 27.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 1 1 1 1 1 1 1 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 1 1 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 15 E V > 0 0 93 0, 0.0 4,-2.6 0, 0.0 5,-0.3 0.000 360.0 360.0 360.0 -77.1 -5.2 -11.3 -13.4 2 16 E K H > + 0 0 140 1,-0.3 4,-1.7 2,-0.2 5,-0.1 0.817 360.0 51.1 -45.4 -37.1 -4.2 -11.0 -17.1 3 17 E E H > S+ 0 0 162 2,-0.2 4,-1.3 1,-0.1 -1,-0.3 0.971 112.4 46.2 -65.7 -52.5 -7.6 -9.4 -17.7 4 18 E F H >> S+ 0 0 79 1,-0.2 4,-2.4 2,-0.2 3,-1.3 0.961 110.7 50.1 -51.5 -64.6 -7.0 -6.9 -14.8 5 19 E L H 3X S+ 0 0 17 -4,-2.6 4,-2.9 1,-0.3 5,-0.2 0.873 104.3 61.9 -42.1 -47.6 -3.5 -5.9 -15.8 6 20 E A H 3X S+ 0 0 57 -4,-1.7 4,-1.2 -5,-0.3 -1,-0.3 0.860 109.2 39.3 -49.4 -45.9 -4.7 -5.3 -19.3 7 21 E K H - 0 0 102 -2,-0.3 3,-1.5 71,-0.1 4,-0.1 -0.936 27.9-114.6-135.1 158.3 0.3 26.1 -5.4 26 40 E L G > S+ 0 0 57 -2,-0.3 3,-1.4 1,-0.3 -1,-0.1 0.896 113.1 58.3 -60.9 -39.4 2.5 26.4 -2.3 27 41 E D G 3 S+ 0 0 96 1,-0.3 -1,-0.3 3,-0.1 22,-0.0 0.474 86.8 75.2 -72.4 -1.3 0.1 28.8 -0.6 28 42 E Q G < S+ 0 0 51 -3,-1.5 21,-1.1 21,-0.1 22,-0.5 0.408 97.4 61.9 -88.4 6.1 -2.8 26.5 -0.8 29 43 E F E < S-A 48 0A 13 -3,-1.4 2,-0.6 19,-0.2 19,-0.2 -0.929 72.4-135.5-134.2 156.0 -1.2 24.6 2.1 30 44 E D E -A 47 0A 75 17,-2.2 17,-1.8 -2,-0.3 2,-0.6 -0.939 25.6-132.6-111.0 121.0 -0.1 25.0 5.7 31 45 E R E +A 46 0A 73 -2,-0.6 15,-0.2 15,-0.2 3,-0.1 -0.620 35.5 164.8 -73.4 117.8 3.4 23.7 6.5 32 46 E I E - 0 0 82 13,-2.1 2,-0.3 -2,-0.6 14,-0.2 0.872 50.7 -29.6-101.8 -55.1 3.0 21.7 9.7 33 47 E K E -A 45 0A 55 12,-1.6 12,-4.0 282,-0.0 2,-0.5 -0.992 55.8 -97.0-166.0 157.4 6.1 19.5 10.3 34 48 E T E -A 44 0A 1 -2,-0.3 282,-0.4 10,-0.3 10,-0.3 -0.699 29.8-178.3 -82.8 125.2 8.9 17.5 8.7 35 49 E L E - 0 0 7 8,-3.4 279,-0.3 -2,-0.5 2,-0.3 0.612 65.8 -19.8 -97.3 -17.8 8.0 13.8 8.4 36 50 E G E -A 43 0A 6 7,-1.4 7,-2.1 277,-0.1 2,-0.3 -0.965 62.4-121.6-175.3 173.1 11.3 12.6 6.9 37 51 E T E +A 42 0A 77 -2,-0.3 5,-0.2 5,-0.2 2,-0.2 -0.950 21.2 177.5-135.1 152.4 14.3 13.8 5.1 38 52 E G - 0 0 48 3,-1.4 315,-0.0 -2,-0.3 4,-0.0 -0.533 44.7 -91.7-132.0-160.7 16.0 13.0 1.8 39 53 E S S S+ 0 0 80 -2,-0.2 3,-0.1 1,-0.1 21,-0.0 0.840 121.2 33.2 -90.2 -36.2 18.9 14.2 -0.2 40 54 E F S S- 0 0 33 1,-0.3 21,-2.8 19,-0.0 2,-0.3 0.588 131.1 -35.4 -96.1 -11.9 17.2 16.8 -2.4 41 55 E G - 0 0 15 19,-0.2 -3,-1.4 23,-0.1 -1,-0.3 -0.977 65.1 -78.8 173.2 179.3 14.7 17.9 0.2 42 56 E R E -A 37 0A 44 -2,-0.3 17,-2.3 -5,-0.2 2,-0.6 -0.669 28.1-127.8-105.3 156.6 12.4 17.1 3.1 43 57 E V E -AB 36 58A 42 -7,-2.1 -8,-3.4 -2,-0.2 -7,-1.4 -0.911 33.0-163.8-102.1 123.3 8.9 15.5 3.2 44 58 E M E -AB 34 57A 6 13,-2.9 13,-3.3 -2,-0.6 2,-0.4 -0.892 24.9-123.6-116.2 142.0 6.6 17.6 5.3 45 59 E L E +AB 33 56A 0 -12,-4.0 -13,-2.1 -2,-0.4 -12,-1.6 -0.651 46.8 178.6 -73.5 129.7 3.2 17.0 6.9 46 60 E V E -AB 31 55A 4 9,-3.4 9,-2.6 -2,-0.4 2,-0.5 -0.893 25.5-135.2-134.4 162.6 1.1 19.7 5.3 47 61 E K E -AB 30 54A 82 -17,-1.8 -17,-2.2 -2,-0.3 2,-1.1 -0.976 14.2-135.5-127.6 124.9 -2.6 20.8 5.4 48 62 E H E >> -AB 29 53A 22 5,-3.5 4,-4.3 -2,-0.5 5,-0.7 -0.642 17.7-156.2 -77.5 99.5 -4.7 21.7 2.4 49 63 E K T 45S+ 0 0 103 -2,-1.1 -1,-0.2 -21,-1.1 -20,-0.1 0.832 87.9 48.0 -43.5 -46.6 -6.4 24.8 3.6 50 64 E E T 45S+ 0 0 114 -22,-0.5 -1,-0.2 1,-0.2 -21,-0.1 0.961 126.0 25.5 -64.8 -53.6 -9.4 24.5 1.3 51 65 E S T 45S- 0 0 56 2,-0.1 -2,-0.2 -3,-0.0 -1,-0.2 0.721 95.3-133.7 -84.3 -22.3 -10.2 20.8 1.9 52 66 E G T <5 + 0 0 34 -4,-4.3 2,-0.2 1,-0.3 -3,-0.2 0.660 58.5 142.7 76.1 14.8 -8.7 20.7 5.4 53 67 E N E < -B 48 0A 82 -5,-0.7 -5,-3.5 1,-0.0 2,-0.4 -0.576 50.0-122.9 -89.8 153.0 -7.0 17.5 4.4 54 68 E H E +B 47 0A 43 -7,-0.2 54,-0.8 -2,-0.2 2,-0.3 -0.763 31.8 176.2 -97.4 139.3 -3.6 16.4 5.4 55 69 E Y E -BC 46 107A 24 -9,-2.6 -9,-3.4 -2,-0.4 2,-0.7 -0.956 33.6-124.4-139.8 157.0 -0.9 15.5 2.9 56 70 E A E -BC 45 106A 11 50,-1.7 50,-3.3 -2,-0.3 2,-0.6 -0.924 31.7-159.0-101.9 110.3 2.8 14.6 2.9 57 71 E M E -BC 44 105A 2 -13,-3.3 -13,-2.9 -2,-0.7 2,-0.6 -0.850 2.7-153.6 -98.0 117.6 4.5 17.1 0.6 58 72 E K E -BC 43 104A 34 46,-3.2 46,-1.8 -2,-0.6 2,-0.4 -0.796 12.6-159.8 -91.4 121.4 7.9 16.1 -0.8 59 73 E I E - C 0 103A 2 -17,-2.3 2,-0.4 -2,-0.6 44,-0.2 -0.861 7.5-170.2-107.0 135.4 10.1 19.1 -1.6 60 74 E L E - C 0 102A 2 42,-2.3 42,-2.1 -2,-0.4 2,-0.7 -0.979 20.9-136.2-127.8 122.8 13.0 19.0 -4.0 61 75 E D E > - C 0 101A 26 -21,-2.8 4,-2.4 -2,-0.4 40,-0.2 -0.649 16.0-149.7 -74.4 114.4 15.6 21.7 -4.4 62 76 E K H > S+ 0 0 4 38,-2.2 4,-1.8 -2,-0.7 -1,-0.2 0.877 94.1 50.7 -54.2 -42.0 16.0 22.0 -8.2 63 77 E Q H > S+ 0 0 79 37,-0.5 4,-2.3 1,-0.2 -1,-0.2 0.892 110.4 48.3 -65.0 -41.2 19.6 23.1 -7.9 64 78 E K H > S+ 0 0 96 2,-0.2 4,-1.6 1,-0.2 -1,-0.2 0.885 108.7 54.8 -66.5 -37.8 20.6 20.2 -5.6 65 79 E V H <>S+ 0 0 0 -4,-2.4 5,-2.9 1,-0.2 6,-0.4 0.894 110.9 46.3 -61.9 -38.3 18.8 17.8 -8.0 66 80 E V H ><5S+ 0 0 43 -4,-1.8 3,-1.3 -5,-0.2 -2,-0.2 0.876 108.5 54.6 -71.4 -38.6 21.0 19.2 -10.8 67 81 E K H 3<5S+ 0 0 167 -4,-2.3 -1,-0.2 1,-0.3 -2,-0.2 0.794 112.6 43.9 -66.8 -26.0 24.2 19.0 -8.8 68 82 E L T 3<5S- 0 0 53 -4,-1.6 -1,-0.3 -5,-0.1 -2,-0.2 0.287 110.4-122.6-100.3 8.6 23.5 15.3 -8.1 69 83 E K T < 5S+ 0 0 154 -3,-1.3 -3,-0.2 1,-0.1 4,-0.2 0.881 71.6 131.6 51.8 45.1 22.6 14.6 -11.7 70 84 E Q >< + 0 0 12 -5,-2.9 4,-2.4 -6,-0.2 5,-0.2 0.173 27.7 113.0-109.8 18.1 19.2 13.3 -10.6 71 85 E I H > S+ 0 0 44 -6,-0.4 4,-2.3 1,-0.2 5,-0.2 0.932 80.1 40.3 -53.0 -57.1 17.2 15.3 -13.1 72 86 E E H > S+ 0 0 125 1,-0.2 4,-2.6 2,-0.2 -1,-0.2 0.887 114.9 52.2 -65.2 -39.8 16.0 12.4 -15.2 73 87 E H H > S+ 0 0 18 2,-0.2 4,-2.3 1,-0.2 -1,-0.2 0.882 110.3 50.6 -63.0 -36.4 15.3 10.2 -12.2 74 88 E T H X S+ 0 0 4 -4,-2.4 4,-2.1 2,-0.2 5,-0.2 0.921 111.1 45.9 -67.5 -44.8 13.2 13.1 -10.7 75 89 E L H X S+ 0 0 12 -4,-2.3 4,-2.7 1,-0.2 5,-0.2 0.895 111.5 54.8 -64.2 -37.6 11.2 13.5 -13.9 76 90 E N H X S+ 0 0 19 -4,-2.6 4,-2.8 1,-0.2 5,-0.3 0.929 107.3 51.0 -57.9 -49.0 10.8 9.7 -14.0 77 91 E E H X S+ 0 0 6 -4,-2.3 4,-2.4 95,-0.2 -2,-0.2 0.921 114.4 39.7 -58.2 -50.3 9.4 9.8 -10.4 78 92 E K H X S+ 0 0 4 -4,-2.1 4,-2.0 2,-0.2 -1,-0.2 0.923 117.1 50.1 -68.3 -42.5 6.8 12.5 -11.0 79 93 E R H < S+ 0 0 60 -4,-2.7 4,-0.3 -5,-0.2 -2,-0.2 0.918 117.3 39.4 -62.4 -44.8 5.8 11.1 -14.4 80 94 E I H >X S+ 0 0 0 -4,-2.8 4,-1.5 -5,-0.2 3,-1.2 0.914 115.2 51.2 -70.4 -45.5 5.4 7.5 -13.2 81 95 E L H 3< S+ 0 0 7 -4,-2.4 11,-0.5 -5,-0.3 -2,-0.2 0.833 106.2 54.6 -64.4 -33.3 3.8 8.4 -9.9 82 96 E Q T 3< S+ 0 0 21 -4,-2.0 -1,-0.3 -5,-0.2 -2,-0.2 0.580 114.6 41.7 -77.6 -7.0 1.1 10.6 -11.4 83 97 E A T <4 S+ 0 0 3 -3,-1.2 -68,-0.2 -4,-0.3 -2,-0.2 0.638 95.3 85.0-114.4 -17.8 0.1 7.8 -13.7 84 98 E V < - 0 0 11 -4,-1.5 2,-0.2 -69,-0.1 -75,-0.0 -0.357 48.3-173.8 -83.7 167.5 0.0 4.6 -11.6 85 99 E N + 0 0 99 -2,-0.1 -73,-0.1 6,-0.1 5,-0.1 -0.674 20.8 138.5-164.6 101.5 -2.8 3.4 -9.5 86 100 E F > - 0 0 10 3,-0.3 3,-1.7 -2,-0.2 53,-0.0 -0.986 56.5-112.1-145.9 152.4 -2.6 0.5 -7.1 87 101 E P T 3 S+ 0 0 30 0, 0.0 -1,-0.1 0, 0.0 206,-0.0 0.816 115.6 43.2 -52.1 -38.6 -3.8 -0.3 -3.6 88 102 E F T 3 S+ 0 0 0 47,-0.1 80,-3.5 79,-0.1 2,-0.5 0.136 95.4 91.9-101.7 22.3 -0.4 -0.5 -2.1 89 103 E L B < S-e 168 0B 2 -3,-1.7 -3,-0.3 78,-0.3 80,-0.2 -0.969 80.2-118.7-117.5 125.5 1.1 2.6 -3.8 90 104 E V - 0 0 4 78,-1.9 2,-0.3 -2,-0.5 -2,-0.1 -0.350 35.6-121.9 -59.7 137.7 0.9 6.0 -2.1 91 105 E K - 0 0 68 -6,-0.0 16,-1.4 1,-0.0 2,-0.9 -0.652 7.4-135.2 -90.6 140.2 -1.2 8.4 -4.3 92 106 E L E -D 106 0A 28 -11,-0.5 14,-0.3 -2,-0.3 3,-0.1 -0.811 22.2-178.9 -92.9 106.1 0.1 11.7 -5.6 93 107 E E E - 0 0 80 12,-1.8 2,-0.3 -2,-0.9 -1,-0.2 0.942 63.7 -6.6 -70.9 -50.9 -2.6 14.3 -5.0 94 108 E F E -D 105 0A 35 11,-1.4 11,-3.2 2,-0.0 2,-0.3 -0.953 56.6-164.4-142.7 163.8 -1.0 17.3 -6.6 95 109 E S E +D 104 0A 2 -2,-0.3 -72,-2.3 9,-0.2 -71,-0.3 -0.988 21.5 142.4-149.3 141.8 2.3 18.4 -8.1 96 110 E F E -D 103 0A 5 7,-1.6 7,-3.2 -2,-0.3 2,-0.3 -0.958 25.6-143.5-165.1 177.6 3.8 21.8 -8.8 97 111 E K E -D 102 0A 65 -2,-0.3 5,-0.2 5,-0.2 2,-0.2 -0.963 7.6-168.4-149.8 163.2 7.0 23.9 -8.8 98 112 E D - 0 0 37 3,-2.3 230,-0.3 -2,-0.3 226,-0.1 -0.684 54.7 -57.0-138.2-165.1 8.3 27.4 -8.1 99 113 E N S S+ 0 0 53 -2,-0.2 229,-2.5 225,-0.2 226,-0.1 0.826 131.5 7.8 -48.7 -38.5 11.5 29.4 -8.6 100 114 E S S S+ 0 0 9 227,-0.2 -38,-2.2 224,-0.1 -37,-0.5 0.538 118.7 55.6-126.3 -6.3 13.6 27.0 -6.7 101 115 E N E -C 61 0A 7 -40,-0.2 -3,-2.3 -39,-0.1 2,-0.4 -0.860 53.7-142.5-132.8 165.4 11.7 23.8 -5.8 102 116 E L E -CD 60 97A 0 -42,-2.1 -42,-2.3 -2,-0.3 2,-0.4 -0.936 26.0-156.6-117.2 142.4 9.7 20.9 -7.1 103 117 E Y E -CD 59 96A 0 -7,-3.2 -7,-1.6 -2,-0.4 2,-0.4 -0.972 17.7-177.7-130.2 139.9 6.7 19.7 -5.1 104 118 E M E -CD 58 95A 0 -46,-1.8 -46,-3.2 -2,-0.4 2,-0.7 -0.961 13.7-157.9-132.3 111.4 4.9 16.4 -4.9 105 119 E V E +CD 57 94A 0 -11,-3.2 -12,-1.8 -2,-0.4 -11,-1.4 -0.842 20.7 177.2 -94.5 112.6 1.9 16.3 -2.6 106 120 E M E -CD 56 92A 11 -50,-3.3 -50,-1.7 -2,-0.7 -14,-0.2 -0.507 39.8 -81.8-104.4 175.2 1.2 12.6 -1.6 107 121 E E E -C 55 0A 60 -16,-1.4 2,-0.5 -52,-0.2 -52,-0.2 -0.560 48.6-115.7 -76.0 146.9 -1.4 11.2 0.7 108 122 E Y - 0 0 36 -54,-0.8 2,-0.8 -2,-0.2 -54,-0.1 -0.738 24.3-160.2 -85.9 125.1 -0.2 11.3 4.3 109 123 E V > - 0 0 9 -2,-0.5 3,-1.4 1,-0.2 51,-0.2 -0.826 7.2-174.3-109.5 92.1 0.2 7.8 5.8 110 124 E A T 3 + 0 0 16 -2,-0.8 202,-0.3 1,-0.3 -1,-0.2 0.749 67.9 83.0 -55.8 -32.4 0.1 8.3 9.5 111 125 E G T 3 S- 0 0 3 49,-0.4 -1,-0.3 1,-0.1 50,-0.2 0.706 91.8-136.4 -48.7 -26.5 1.0 4.7 10.3 112 126 E G < - 0 0 0 -3,-1.4 48,-2.5 48,-0.1 2,-0.2 -0.269 32.6 -39.6 94.9 177.2 4.7 5.4 9.9 113 127 E E B > -F 159 0B 14 199,-0.5 4,-1.5 46,-0.2 3,-0.2 -0.552 40.8-132.3 -82.0 143.2 7.6 3.6 8.2 114 128 E M H > S+ 0 0 1 44,-2.9 4,-3.5 41,-0.8 5,-0.3 0.937 108.4 65.9 -56.6 -41.8 7.9 -0.2 8.4 115 129 E F H > S+ 0 0 16 40,-1.2 4,-2.7 43,-0.3 5,-0.3 0.885 99.5 47.7 -43.1 -56.5 11.5 0.6 9.4 116 130 E S H > S+ 0 0 29 -3,-0.2 4,-2.2 1,-0.2 -1,-0.2 0.956 117.6 40.4 -52.5 -58.7 10.6 2.2 12.7 117 131 E H H X S+ 0 0 32 -4,-1.5 4,-2.8 1,-0.2 5,-0.3 0.896 116.7 49.9 -59.3 -44.6 8.2 -0.5 13.8 118 132 E L H X S+ 0 0 0 -4,-3.5 4,-0.9 -5,-0.2 -1,-0.2 0.872 112.6 45.3 -65.0 -39.2 10.5 -3.3 12.6 119 133 E R H < S+ 0 0 54 -4,-2.7 -1,-0.2 -5,-0.3 -2,-0.2 0.883 116.3 47.6 -72.7 -37.8 13.6 -2.0 14.2 120 134 E R H < S+ 0 0 230 -4,-2.2 -2,-0.2 -5,-0.3 -3,-0.2 0.922 116.7 39.5 -68.5 -48.9 11.7 -1.3 17.5 121 135 E I H < S- 0 0 100 -4,-2.8 2,-1.1 -5,-0.2 -1,-0.2 0.606 103.6-134.2 -77.9 -13.2 9.9 -4.7 17.7 122 136 E G < - 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