==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=22-JUL-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER CONTRACTILE PROTEIN/CONTRACTILE PROTEIN 04-DEC-05 2F8V . COMPND 2 MOLECULE: N2B-TITIN ISOFORM; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR N.PINOTSIS,M.PETOUKHOV,S.LANGE,D.SVERGUN,P.ZOU,M.GAUTEL,M.WI . 951 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 46259.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 674 70.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 38 4.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 454 47.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 28 2.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 3 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 99 10.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 66 6.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 8 2 9 7 28 0 0 8 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 7 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A T 0 0 178 0, 0.0 2,-0.3 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 100.2 -14.5 45.8 13.9 2 3 A T - 0 0 61 82,-0.0 2,-0.5 28,-0.0 407,-0.0 -0.712 360.0-151.7 -74.7 140.8 -12.3 42.7 14.0 3 4 A Q E -A 31 0A 92 28,-3.2 28,-1.8 -2,-0.3 83,-0.1 -0.961 8.8-125.9-128.8 116.1 -9.1 44.0 15.7 4 5 A A E - 0 0 8 -2,-0.5 83,-0.1 26,-0.2 403,-0.1 -0.135 45.3 -88.0 -50.6 151.3 -5.7 42.5 15.1 5 6 A P E + 0 0 3 0, 0.0 2,-0.3 0, 0.0 25,-0.2 -0.323 55.5 169.5 -65.3 144.6 -3.9 41.5 18.3 6 7 A T E -A 29 0A 27 23,-2.1 23,-2.5 -3,-0.1 2,-0.4 -0.985 31.0-117.4-154.7 155.3 -1.8 44.2 20.1 7 8 A F E +A 28 0A 9 -2,-0.3 21,-0.2 21,-0.2 82,-0.0 -0.820 19.9 178.6-100.5 135.4 -0.1 44.7 23.4 8 9 A T E S+ 0 0 67 19,-1.8 -1,-0.1 -2,-0.4 20,-0.1 0.576 93.2 16.7-101.3 -17.9 -1.1 47.4 25.9 9 10 A Q E S-A 27 0A 61 18,-1.2 18,-2.2 -3,-0.1 -1,-0.4 -0.810 84.2-161.1-153.4 106.4 1.6 46.0 28.2 10 11 A P - 0 0 17 0, 0.0 15,-0.1 0, 0.0 393,-0.1 -0.212 36.8 -74.2 -79.0 173.2 4.3 43.8 26.7 11 12 A L - 0 0 1 391,-0.2 2,-0.3 68,-0.1 80,-0.1 -0.282 48.9-160.0 -58.1 156.2 6.7 41.5 28.4 12 13 A Q - 0 0 32 13,-0.2 13,-0.2 -3,-0.1 80,-0.1 -0.963 30.0 -84.0-140.9 150.9 9.5 43.2 30.3 13 14 A S - 0 0 7 -2,-0.3 2,-0.4 78,-0.1 81,-0.2 -0.038 41.1-152.4 -54.0 161.3 12.8 41.7 31.4 14 15 A V E -d 94 0B 28 79,-1.9 81,-1.9 407,-0.1 2,-0.4 -0.966 12.0-172.4-141.8 122.2 12.9 39.8 34.7 15 16 A V E +d 95 0B 18 -2,-0.4 2,-0.3 79,-0.2 81,-0.2 -0.925 21.1 161.7-109.4 141.2 15.9 39.3 37.0 16 17 A V E -d 96 0B 2 79,-1.8 81,-1.9 -2,-0.4 2,-0.2 -0.996 38.5 -92.0-161.3 155.3 15.5 36.9 39.9 17 18 A L E > -d 97 0B 13 -2,-0.3 3,-3.1 79,-0.2 81,-0.2 -0.420 51.4 -95.7 -74.5 132.4 17.3 34.8 42.6 18 19 A E T 3 S+ 0 0 74 79,-4.1 52,-0.2 1,-0.3 -1,-0.1 -0.130 114.0 22.5 -38.3 127.1 18.2 31.3 41.9 19 20 A G T 3 S+ 0 0 5 50,-2.2 -1,-0.3 1,-0.3 51,-0.1 0.262 101.4 106.3 91.7 -11.8 15.4 29.2 43.4 20 21 A S S < S- 0 0 3 -3,-3.1 49,-1.6 48,-0.1 2,-0.5 -0.190 77.8 -87.9 -86.1-175.1 12.7 31.8 43.4 21 22 A T - 0 0 56 47,-0.2 2,-0.3 817,-0.1 -4,-0.1 -0.832 35.8-160.7 -95.0 131.4 9.7 32.3 41.3 22 23 A A E - B 0 66A 2 44,-1.9 44,-2.4 -2,-0.5 2,-0.3 -0.846 9.0-167.0-105.6 151.9 10.0 34.2 38.0 23 24 A T E - B 0 65A 52 -2,-0.3 2,-0.4 42,-0.2 42,-0.2 -0.937 13.7-166.0-146.5 116.4 6.9 35.6 36.3 24 25 A F E + B 0 64A 1 40,-2.3 40,-2.3 -2,-0.3 2,-0.3 -0.830 19.7 177.0 -97.5 143.5 6.3 37.0 32.9 25 26 A E E + B 0 63A 54 -2,-0.4 2,-0.3 38,-0.2 38,-0.2 -0.996 6.8 167.0-145.9 143.2 3.0 38.9 32.4 26 27 A A E - B 0 62A 0 36,-2.3 36,-3.2 -2,-0.3 2,-0.5 -0.987 27.5-132.3-154.2 152.7 1.5 40.9 29.6 27 28 A H E -AB 9 61A 54 -18,-2.2 -19,-1.8 -2,-0.3 -18,-1.2 -0.939 24.7-170.4-109.1 131.0 -1.9 42.3 28.6 28 29 A I E -A 7 0A 10 32,-3.0 2,-0.3 -2,-0.5 -21,-0.2 -0.957 6.1-165.9-122.9 138.1 -3.2 41.7 25.2 29 30 A S E +A 6 0A 39 -23,-2.5 -23,-2.1 -2,-0.4 2,-0.3 -0.920 30.0 115.7-118.2 152.8 -6.2 43.2 23.4 30 31 A G E - 0 0 19 -2,-0.3 3,-0.2 -25,-0.2 -26,-0.2 -0.950 48.2 -99.8 167.3 175.5 -7.9 41.9 20.3 31 32 A F E S+A 3 0A 134 -28,-1.8 -28,-3.2 1,-0.6 2,-0.2 -0.754 104.5 30.5-177.4 118.0 -11.0 40.5 18.8 32 33 A P S S- 0 0 69 0, 0.0 -1,-0.6 0, 0.0 53,-0.1 0.625 114.8-101.6 -69.6 160.9 -11.7 37.8 18.2 33 34 A V - 0 0 113 -3,-0.2 -2,-0.1 -2,-0.2 49,-0.0 -0.274 43.6-115.5 -56.4 127.3 -9.4 36.8 21.1 34 35 A P - 0 0 12 0, 0.0 2,-0.6 0, 0.0 49,-0.2 -0.038 14.1-115.3 -58.6 159.2 -6.0 35.5 19.9 35 36 A E E -E 82 0B 151 47,-2.0 47,-1.5 2,-0.0 2,-0.3 -0.907 41.0-155.4 -93.7 117.2 -4.5 32.1 20.3 36 37 A V E +E 81 0B 32 -2,-0.6 2,-0.3 45,-0.2 45,-0.2 -0.764 18.8 172.6-102.4 147.1 -1.6 32.7 22.6 37 38 A S E -E 80 0B 38 43,-2.0 43,-2.6 -2,-0.3 2,-0.3 -0.993 19.3-139.1-150.5 145.4 1.6 30.6 22.8 38 39 A W E -E 79 0B 10 -2,-0.3 7,-1.8 41,-0.2 2,-0.3 -0.781 16.2-168.6-107.3 147.7 4.9 31.0 24.7 39 40 A F E -EF 78 44B 42 39,-2.8 39,-2.3 -2,-0.3 2,-0.4 -0.937 11.9-156.3-130.3 156.4 8.4 30.2 23.5 40 41 A R E > S-EF 77 43B 60 3,-3.1 3,-2.9 -2,-0.3 37,-0.2 -0.999 81.3 -18.5-127.3 139.8 11.9 30.0 25.0 41 42 A D T 3 S- 0 0 78 35,-2.0 36,-0.1 -2,-0.4 3,-0.1 0.795 126.3 -56.9 34.7 41.2 15.0 30.4 22.9 42 43 A G T 3 S+ 0 0 54 34,-0.3 2,-0.3 1,-0.2 -1,-0.3 0.156 119.6 105.7 83.3 -21.6 12.9 29.9 19.8 43 44 A Q E < S-F 40 0B 140 -3,-2.9 -3,-3.1 1,-0.1 -1,-0.2 -0.723 80.2-101.6 -94.8 143.5 11.7 26.4 21.0 44 45 A V E -F 39 0B 84 -2,-0.3 2,-0.3 -5,-0.2 -5,-0.2 -0.313 36.0-118.8 -69.4 147.1 8.1 26.1 22.3 45 46 A I + 0 0 15 -7,-1.8 2,-0.3 3,-0.1 -7,-0.2 -0.644 43.8 150.1 -88.7 139.0 7.6 25.9 26.0 46 47 A S >> - 0 0 45 -2,-0.3 4,-2.2 1,-0.0 3,-0.8 -0.963 61.7 -95.2-155.6 165.1 6.0 22.9 27.7 47 48 A T T 34 S+ 0 0 97 -2,-0.3 5,-0.1 1,-0.2 6,-0.1 0.757 121.2 64.0 -56.1 -20.7 6.1 21.0 31.0 48 49 A S T 34 S+ 0 0 105 1,-0.2 -1,-0.2 2,-0.1 -3,-0.1 0.916 112.2 28.7 -73.5 -40.8 8.7 18.8 29.3 49 50 A T T <4 S+ 0 0 79 -3,-0.8 -2,-0.2 1,-0.2 -1,-0.2 0.607 133.8 34.1 -98.0 -12.2 11.3 21.4 28.7 50 51 A L S >< S- 0 0 5 -4,-2.2 3,-2.1 3,-0.1 -1,-0.2 -0.628 86.8-144.6-138.9 83.4 10.4 23.6 31.8 51 52 A P T 3 S+ 0 0 105 0, 0.0 -3,-0.1 0, 0.0 -4,-0.1 -0.112 79.7 9.0 -50.9 130.1 9.2 21.3 34.6 52 53 A G T 3 S+ 0 0 46 1,-0.2 -5,-0.1 -5,-0.1 13,-0.1 0.379 83.4 156.0 82.5 -5.4 6.4 22.8 36.7 53 54 A V < - 0 0 16 -3,-2.1 2,-0.4 -7,-0.1 -1,-0.2 -0.208 28.6-150.8 -52.2 144.8 5.6 25.9 34.5 54 55 A Q E -C 65 0A 128 11,-2.1 11,-2.7 2,-0.0 2,-0.3 -0.973 10.8-167.2-120.2 136.3 2.1 27.3 34.9 55 56 A I E +C 64 0A 93 -2,-0.4 2,-0.3 9,-0.2 9,-0.2 -0.935 10.0 168.3-126.3 149.6 0.2 29.1 32.1 56 57 A S E +C 63 0A 55 7,-1.4 7,-2.1 -2,-0.3 2,-0.2 -0.989 12.7 180.0-158.0 147.4 -2.9 31.2 32.1 57 58 A F E +C 62 0A 71 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.739 26.5 136.5-155.9 94.8 -4.8 33.5 29.8 58 59 A S E > +C 61 0A 93 3,-1.6 3,-0.8 -2,-0.2 -2,-0.0 -0.992 64.3 7.6-145.9 141.5 -8.0 35.2 30.8 59 60 A D T 3 S- 0 0 167 -2,-0.3 -1,-0.1 1,-0.2 3,-0.1 0.916 127.9 -54.5 59.6 49.8 -9.4 38.7 30.5 60 61 A G T 3 S+ 0 0 31 1,-0.2 -32,-3.0 -33,-0.1 2,-0.5 0.557 113.4 117.9 69.2 9.7 -6.7 40.1 28.2 61 62 A R E < -BC 27 58A 104 -3,-0.8 -3,-1.6 -34,-0.3 2,-0.4 -0.932 43.5-170.7-119.5 115.8 -4.1 39.0 30.7 62 63 A A E +BC 26 57A 0 -36,-3.2 -36,-2.3 -2,-0.5 2,-0.3 -0.850 10.9 176.7-106.0 145.3 -1.4 36.5 29.8 63 64 A K E -BC 25 56A 63 -7,-2.1 -7,-1.4 -2,-0.4 2,-0.5 -0.963 17.3-168.4-157.7 127.2 0.9 35.1 32.5 64 65 A L E -BC 24 55A 0 -40,-2.3 -40,-2.3 -2,-0.3 2,-0.4 -0.984 12.5-164.0-123.1 126.5 3.7 32.5 32.6 65 66 A T E -BC 23 54A 23 -11,-2.7 -11,-2.1 -2,-0.5 -42,-0.2 -0.924 8.9-166.5-116.0 127.0 5.0 31.2 36.0 66 67 A I E > -B 22 0A 3 -44,-2.4 -44,-1.9 -2,-0.4 3,-0.5 -0.945 10.7-159.3-113.2 116.5 8.3 29.3 36.6 67 68 A P T 3 S+ 0 0 47 0, 0.0 2,-0.4 0, 0.0 -1,-0.2 0.954 75.4 7.7 -60.3 -68.8 8.4 27.8 40.2 68 69 A A T 3 S- 0 0 66 -47,-0.1 -47,-0.2 2,-0.0 -48,-0.1 -0.557 82.4-161.0-121.5 67.4 12.0 27.1 41.2 69 70 A V < - 0 0 2 -49,-1.6 -50,-2.2 -3,-0.5 2,-0.4 0.027 3.9-160.4 -56.8 156.7 14.1 28.6 38.5 70 71 A T > - 0 0 57 -52,-0.2 3,-5.8 -51,-0.1 4,-0.3 -0.999 35.6-103.9-135.8 142.1 17.7 27.6 37.9 71 72 A K G > S+ 0 0 100 -2,-0.4 3,-2.6 1,-0.3 25,-0.2 0.770 121.2 63.1 -25.2 -52.9 20.6 29.4 36.1 72 73 A A G 3 S+ 0 0 81 1,-0.3 -1,-0.3 -3,-0.0 -3,-0.0 0.763 91.2 69.2 -51.7 -25.4 20.1 27.0 33.2 73 74 A N G < S+ 0 0 26 -3,-5.8 -1,-0.3 2,-0.0 -2,-0.2 0.551 73.9 113.7 -69.9 -10.3 16.6 28.5 32.8 74 75 A S < + 0 0 31 -3,-2.6 2,-0.2 -4,-0.3 21,-0.2 -0.271 41.0 92.5 -65.0 156.0 17.9 31.9 31.6 75 76 A G E S- G 0 94B 13 19,-1.4 19,-1.5 2,-0.0 2,-0.5 -0.866 75.4 -41.9 146.5 179.6 17.2 32.9 28.0 76 77 A R E - G 0 93B 100 -2,-0.2 -35,-2.0 17,-0.2 2,-0.5 -0.653 47.7-159.4 -82.6 126.1 14.8 34.8 25.7 77 78 A Y E -EG 40 92B 0 15,-4.2 15,-2.4 -2,-0.5 2,-0.3 -0.898 8.7-169.8-108.9 129.7 11.1 34.4 26.4 78 79 A S E -EG 39 91B 23 -39,-2.3 -39,-2.8 -2,-0.5 2,-0.4 -0.858 12.0-162.4-116.6 149.9 8.5 35.1 23.7 79 80 A L E -EG 38 90B 0 11,-1.6 11,-1.8 -2,-0.3 2,-0.4 -0.999 12.7-165.6-125.8 129.2 4.7 35.4 23.8 80 81 A K E -EG 37 89B 69 -43,-2.6 -43,-2.0 -2,-0.4 2,-0.4 -0.964 6.5-174.2-119.5 137.0 2.8 35.2 20.5 81 82 A A E +EG 36 88B 0 7,-2.3 7,-1.1 -2,-0.4 2,-0.3 -0.993 12.2 171.5-129.2 125.1 -0.9 36.2 19.9 82 83 A T E +EG 35 87B 38 -47,-1.5 -47,-2.0 -2,-0.4 2,-0.2 -0.870 9.8 152.1-129.6 166.3 -2.7 35.6 16.6 83 84 A N E > - G 0 86B 26 3,-1.9 3,-1.2 -2,-0.3 -50,-0.1 -0.924 62.5 -66.5-173.5 176.9 -6.2 35.7 15.0 84 85 A G T 3 S+ 0 0 84 1,-0.3 3,-0.1 -2,-0.2 -80,-0.1 0.734 128.0 48.5 -46.6 -31.7 -7.8 36.3 11.6 85 86 A S T 3 S- 0 0 5 1,-0.2 323,-2.0 -53,-0.1 324,-1.0 0.815 123.0 -66.0 -82.3 -34.4 -6.6 39.9 11.3 86 87 A G E < -HG 407 83B 7 -3,-1.2 -3,-1.9 321,-0.2 2,-0.2 -0.972 47.0 -98.1 173.1-162.0 -3.0 39.4 12.3 87 88 A Q E -HG 406 82B 82 319,-1.0 319,-1.6 -2,-0.3 2,-0.3 -0.734 16.8-151.0-136.7-174.3 -0.5 38.5 15.0 88 89 A A E -HG 405 81B 1 -7,-1.1 -7,-2.3 -2,-0.2 2,-0.4 -0.892 2.0-162.7-166.2 134.5 1.8 39.9 17.6 89 90 A T E -HG 404 80B 30 315,-3.4 315,-2.8 -2,-0.3 2,-0.4 -0.948 3.2-173.4-123.0 143.2 5.1 39.0 19.3 90 91 A S E -HG 403 79B 0 -11,-1.8 -11,-1.6 -2,-0.4 2,-0.4 -0.985 12.3-163.7-135.7 123.3 6.5 40.4 22.5 91 92 A T E +HG 402 78B 20 311,-3.6 311,-2.0 -2,-0.4 2,-0.3 -0.872 13.8 161.5-120.6 141.4 10.0 39.3 23.6 92 93 A A E - G 0 77B 0 -15,-2.4 -15,-4.2 -2,-0.4 2,-0.3 -0.972 28.5-116.3-152.6 165.2 11.9 39.5 26.9 93 94 A E E - 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