==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=30-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 05-JAN-06 2FL1 . COMPND 2 MOLECULE: RED FLUORESCENT PROTEIN ZOANRFP; . SOURCE 2 ORGANISM_SCIENTIFIC: ZOANTHUS SP.; . AUTHOR V.PLETNEV,N.PLETNEVA,V.MARTYNOV,T.TIKHONOVA,B.POPOV, . 900 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 38080.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 666 74.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 45.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 88 9.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 73 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 27 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 16 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 0 4 0 8 1 5 3 7 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A S 0 0 118 0, 0.0 2,-1.6 0, 0.0 5,-0.1 0.000 360.0 360.0 360.0 159.0 -67.4 1.1 -2.2 2 5 A A B > -A 5 0A 63 3,-0.5 3,-1.1 1,-0.3 80,-0.0 -0.627 360.0 -32.2 -80.5 88.4 -68.1 0.1 -5.8 3 6 A H T 3 S- 0 0 80 -2,-1.6 -1,-0.3 1,-0.2 3,-0.1 0.658 109.8 -71.9 72.6 17.1 -68.4 -3.7 -5.2 4 7 A G T 3 S+ 0 0 7 1,-0.3 2,-0.2 78,-0.1 -1,-0.2 0.535 103.5 126.4 77.4 5.7 -65.8 -3.3 -2.4 5 8 A L B < -A 2 0A 5 -3,-1.1 -3,-0.5 74,-0.1 -1,-0.3 -0.659 35.1-177.0 -98.1 153.1 -63.0 -2.7 -4.9 6 9 A T - 0 0 48 -2,-0.2 3,-0.3 -5,-0.1 29,-0.2 -0.848 39.5-105.6-137.4 173.0 -60.5 0.2 -5.1 7 10 A D S S+ 0 0 104 -2,-0.3 2,-0.2 1,-0.2 29,-0.1 0.365 110.2 45.5 -87.0 7.1 -57.7 1.2 -7.5 8 11 A D S S+ 0 0 100 25,-0.1 -1,-0.2 2,-0.0 25,-0.2 -0.611 76.8 151.8-149.0 83.1 -54.9 0.2 -5.2 9 12 A M E -B 32 0B 0 23,-2.5 23,-1.7 -3,-0.3 2,-0.3 -0.597 34.1-134.7-110.9 170.4 -55.4 -3.2 -3.6 10 13 A T E -Bc 31 114B 68 103,-1.7 105,-2.4 21,-0.2 2,-0.3 -0.759 18.6-149.1-117.1 166.0 -53.2 -6.0 -2.3 11 14 A M E -Bc 30 115B 3 19,-1.7 19,-1.1 -2,-0.3 2,-0.5 -0.957 6.1-147.7-136.6 156.1 -53.6 -9.7 -2.9 12 15 A H E -Bc 29 116B 64 103,-2.6 105,-3.2 -2,-0.3 2,-0.4 -0.990 19.2-163.7-127.0 124.5 -52.9 -12.9 -1.1 13 16 A F E +Bc 28 117B 0 15,-3.2 15,-1.9 -2,-0.5 2,-0.3 -0.887 17.9 165.8-116.0 138.8 -51.9 -16.1 -3.0 14 17 A R E +Bc 27 118B 59 103,-1.5 105,-2.3 -2,-0.4 2,-0.4 -0.894 9.6 178.5-147.9 115.5 -51.8 -19.7 -2.1 15 18 A M E -Bc 26 119B 0 11,-3.1 11,-2.9 -2,-0.3 2,-0.4 -0.934 7.6-170.9-118.9 142.5 -51.4 -22.5 -4.7 16 19 A E E +Bc 25 120B 82 103,-1.9 105,-1.6 -2,-0.4 2,-0.3 -0.993 26.4 140.0-128.6 135.7 -51.2 -26.2 -4.1 17 20 A G E -Bc 24 121B 8 7,-2.3 7,-1.7 -2,-0.4 2,-0.3 -0.952 38.0-140.1-162.8-178.1 -50.4 -28.6 -6.9 18 21 A C E -Bc 23 122B 29 103,-2.2 105,-1.8 -2,-0.3 2,-0.5 -0.916 8.1-168.5-158.5 126.2 -48.6 -31.6 -8.4 19 22 A V E > S-Bc 22 123B 4 3,-1.8 3,-1.9 -2,-0.3 105,-0.2 -0.969 80.7 -23.2-121.7 114.4 -47.0 -32.0 -11.8 20 23 A D T 3 S- 0 0 26 103,-2.0 -1,-0.1 -2,-0.5 104,-0.1 0.856 130.0 -46.2 54.0 39.9 -46.1 -35.6 -12.7 21 24 A G T 3 S+ 0 0 42 102,-0.3 2,-0.9 1,-0.2 -1,-0.3 0.140 109.7 121.9 93.6 -21.2 -46.0 -36.6 -9.1 22 25 A H E < -B 19 0B 62 -3,-1.9 -3,-1.8 26,-0.0 -1,-0.2 -0.672 49.7-155.9 -82.9 105.6 -44.0 -33.7 -7.8 23 26 A K E +B 18 0B 161 -2,-0.9 2,-0.3 -5,-0.2 -5,-0.2 -0.349 19.8 164.9 -75.0 160.0 -46.0 -31.9 -5.1 24 27 A F E -B 17 0B 4 -7,-1.7 -7,-2.3 -2,-0.1 2,-0.4 -0.971 24.5-149.9-169.2 158.5 -45.5 -28.3 -4.3 25 28 A V E -BD 16 47B 11 22,-2.3 22,-1.6 -2,-0.3 21,-1.2 -0.992 12.9-166.1-137.5 142.5 -47.1 -25.3 -2.5 26 29 A I E -BD 15 45B 0 -11,-2.9 -11,-3.1 -2,-0.4 2,-0.3 -0.992 5.4-178.0-133.4 139.7 -46.7 -21.7 -3.4 27 30 A E E +BD 14 44B 69 17,-2.1 17,-2.1 -2,-0.4 2,-0.3 -0.915 17.8 133.8-130.9 158.9 -47.6 -18.5 -1.4 28 31 A G E -BD 13 43B 13 -15,-1.9 -15,-3.2 -2,-0.3 2,-0.2 -0.983 29.9-140.5 179.5-176.1 -47.3 -14.9 -2.3 29 32 A N E +BD 12 42B 90 13,-1.6 13,-3.1 -2,-0.3 2,-0.3 -0.817 18.6 164.0-150.4-173.2 -48.9 -11.5 -2.4 30 33 A G E -BD 11 41B 7 -19,-1.1 -19,-1.7 -2,-0.2 2,-0.3 -0.987 26.1-116.2 174.8-171.0 -49.1 -8.4 -4.6 31 34 A N E +BD 10 40B 68 9,-1.5 9,-3.1 -2,-0.3 2,-0.3 -0.967 32.0 156.8-149.1 158.3 -50.9 -5.2 -5.4 32 35 A G E -BD 9 39B 4 -23,-1.7 -23,-2.5 -2,-0.3 7,-0.2 -0.989 41.5-114.0-173.8 170.0 -52.9 -4.0 -8.4 33 36 A N E > > - D 0 38B 20 5,-2.5 5,-1.8 -2,-0.3 3,-1.4 -0.915 21.0-169.3-119.0 99.7 -55.5 -1.7 -9.9 34 37 A P G > 5S+ 0 0 0 0, 0.0 3,-2.0 0, 0.0 34,-0.5 0.897 85.0 62.7 -57.3 -38.4 -58.5 -3.7 -11.0 35 38 A F G 3 5S+ 0 0 86 1,-0.3 35,-0.1 -29,-0.2 -28,-0.1 0.714 111.8 36.8 -61.8 -20.5 -59.9 -0.7 -12.8 36 39 A K G < 5S- 0 0 108 -3,-1.4 33,-0.3 2,-0.2 -1,-0.3 0.149 109.4-121.2-114.7 15.9 -56.9 -0.6 -15.2 37 40 A G T < 5 + 0 0 0 -3,-2.0 181,-2.2 31,-0.4 2,-0.4 0.865 68.4 134.7 45.3 47.6 -56.5 -4.4 -15.4 38 41 A K E < +DE 33 217B 73 -5,-1.8 -5,-2.5 179,-0.2 2,-0.3 -0.984 25.8 166.0-130.7 132.0 -53.0 -4.1 -14.0 39 42 A Q E -DE 32 216B 0 177,-2.1 177,-2.0 -2,-0.4 2,-0.5 -0.998 26.4-148.5-152.0 148.0 -51.4 -6.3 -11.3 40 43 A F E -DE 31 215B 66 -9,-3.1 -9,-1.5 -2,-0.3 2,-0.4 -0.957 19.5-166.6-116.4 124.3 -48.1 -7.2 -9.7 41 44 A I E -DE 30 214B 2 173,-3.1 173,-2.5 -2,-0.5 2,-0.6 -0.928 8.3-166.8-117.3 131.2 -47.6 -10.7 -8.3 42 45 A N E -DE 29 213B 51 -13,-3.1 -13,-1.6 -2,-0.4 2,-0.3 -0.950 16.5-176.9-118.8 112.9 -44.8 -11.9 -6.0 43 46 A L E -DE 28 212B 4 169,-2.8 169,-1.9 -2,-0.6 2,-0.3 -0.794 11.4-174.7-113.5 153.5 -44.6 -15.7 -5.6 44 47 A C E -DE 27 211B 29 -17,-2.1 -17,-2.1 -2,-0.3 2,-0.9 -0.993 26.8-126.5-145.4 133.3 -42.4 -18.0 -3.5 45 48 A V E +D 26 0B 6 165,-2.6 165,-0.4 -2,-0.3 -19,-0.2 -0.717 31.0 169.9 -83.4 109.8 -42.1 -21.8 -3.5 46 49 A I E + 0 0 72 -21,-1.2 2,-0.3 -2,-0.9 -20,-0.2 0.582 68.1 31.0 -95.8 -10.0 -42.6 -22.8 0.2 47 50 A E E S+D 25 0B 111 -22,-1.6 -22,-2.3 1,-0.0 -1,-0.2 -0.956 119.0 17.0-151.1 128.3 -42.9 -26.6 -0.6 48 51 A G S S- 0 0 30 -2,-0.3 -2,-0.1 1,-0.3 -22,-0.0 0.424 89.8-169.8 94.6 0.4 -41.4 -28.7 -3.3 49 52 A G + 0 0 46 -24,-0.1 -1,-0.3 1,-0.1 2,-0.1 -0.413 59.2 45.6 -74.2 149.3 -38.8 -26.2 -4.3 50 53 A P S S- 0 0 86 0, 0.0 -1,-0.1 0, 0.0 -2,-0.0 0.500 89.9-135.0 -83.6 139.3 -36.9 -26.0 -6.4 51 54 A L - 0 0 0 1,-0.2 4,-0.1 -2,-0.1 -2,-0.1 -0.320 3.8-149.3 -58.5 131.8 -39.5 -26.9 -9.0 52 55 A P S S+ 0 0 50 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.626 80.4 61.9 -77.3 -14.2 -38.3 -29.4 -11.5 53 56 A F S S- 0 0 1 1,-0.1 80,-0.2 81,-0.1 79,-0.1 -0.836 103.8 -83.4-112.4 151.2 -40.6 -27.9 -14.2 54 57 A S > - 0 0 7 78,-1.8 3,-1.1 -2,-0.3 4,-0.2 -0.202 30.3-136.1 -52.8 137.6 -40.5 -24.4 -15.7 55 58 A E G >> S+ 0 0 12 1,-0.2 3,-1.2 2,-0.1 4,-0.7 0.637 93.0 82.7 -71.0 -12.1 -42.4 -21.9 -13.5 56 59 A D G 34 S+ 0 0 1 1,-0.2 3,-0.4 2,-0.2 4,-0.4 0.786 77.9 65.1 -62.9 -30.2 -44.0 -20.5 -16.7 57 60 A I G <4 S+ 0 0 0 -3,-1.1 -1,-0.2 1,-0.2 3,-0.2 0.722 102.5 49.2 -68.0 -18.8 -46.7 -23.1 -16.9 58 61 A L T X4 S+ 0 0 0 -3,-1.2 3,-2.3 -4,-0.2 4,-0.4 0.693 84.4 94.3 -90.8 -23.7 -48.2 -21.9 -13.6 59 62 A S G >< S+ 0 0 4 -4,-0.7 3,-1.1 -3,-0.4 -1,-0.2 0.747 77.9 54.4 -41.2 -45.4 -48.3 -18.2 -14.5 60 63 A A G 3 S+ 0 0 22 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.342 95.0 72.1 -78.9 10.2 -51.9 -18.0 -15.9 61 64 A A G < 0 0 0 -3,-2.3 -1,-0.2 1,-0.1 -2,-0.2 0.669 360.0 360.0 -94.6 -18.3 -53.3 -19.4 -12.7 62 65 A F < 0 0 5 -3,-1.1 -2,-0.2 -4,-0.4 -1,-0.1 0.696 360.0 360.0 -92.9 360.0 -52.6 -16.3 -10.6 63 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 64 69 A N > 0 0 9 0, 0.0 3,-1.9 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 91.4 -57.8 -13.1 -11.6 65 70 A R T 3 + 0 0 8 1,-0.3 3,-0.2 2,-0.0 14,-0.2 0.484 360.0 85.9 -68.4 -2.6 -60.1 -11.5 -14.2 66 71 A L T 3 S+ 0 0 0 1,-0.3 2,-1.8 12,-0.1 -29,-0.3 0.848 80.0 69.8 -64.4 -30.4 -60.9 -8.7 -11.8 67 72 A F S < S+ 0 0 0 -3,-1.9 2,-0.4 -30,-0.1 -1,-0.3 -0.482 75.1 109.3 -87.4 66.9 -57.8 -7.2 -13.2 68 73 A T - 0 0 0 -2,-1.8 2,-0.9 -34,-0.5 -31,-0.4 -0.982 68.6-132.1-138.8 123.6 -59.3 -6.6 -16.6 69 74 A E B -f 219 0B 78 149,-2.5 151,-2.7 -2,-0.4 -34,-0.1 -0.720 30.7-166.1 -81.3 112.9 -60.0 -3.0 -17.7 70 75 A Y - 0 0 29 -2,-0.9 2,-0.2 149,-0.2 6,-0.1 -0.803 15.1-132.6-101.9 140.4 -63.5 -3.2 -19.0 71 76 A P > - 0 0 56 0, 0.0 3,-2.4 0, 0.0 150,-0.0 -0.628 29.1-112.1 -85.7 149.3 -65.1 -0.4 -21.1 72 77 A E T 3 S+ 0 0 206 1,-0.3 4,-0.1 -2,-0.2 -2,-0.0 0.678 113.2 69.2 -55.8 -16.1 -68.7 0.6 -20.1 73 78 A G T 3 S+ 0 0 76 2,-0.1 2,-0.4 1,-0.1 -1,-0.3 0.520 95.0 59.2 -81.5 -5.9 -70.0 -0.9 -23.3 74 79 A I S < S- 0 0 17 -3,-2.4 2,-0.1 116,-0.1 146,-0.1 -0.990 92.1-114.4-125.2 129.8 -69.3 -4.4 -22.1 75 80 A V - 0 0 28 -2,-0.4 2,-1.3 114,-0.1 3,-0.2 -0.412 23.1-136.9 -62.0 130.2 -70.8 -5.9 -19.0 76 81 A D > + 0 0 21 1,-0.2 4,-2.0 -2,-0.1 5,-0.2 -0.722 25.4 178.9 -93.2 89.0 -68.0 -6.7 -16.4 77 82 A Y H > S+ 0 0 2 -2,-1.3 4,-0.8 1,-0.2 -1,-0.2 0.841 77.4 50.9 -57.6 -37.1 -69.1 -10.1 -15.3 78 83 A F H >4 S+ 0 0 0 2,-0.2 3,-0.7 1,-0.2 4,-0.3 0.942 111.5 41.8 -71.6 -50.9 -66.2 -10.3 -12.9 79 84 A K H >4 S+ 0 0 14 1,-0.2 3,-1.2 2,-0.2 -1,-0.2 0.820 109.8 61.2 -67.6 -27.4 -66.4 -7.0 -11.1 80 85 A N H 3< S+ 0 0 62 -4,-2.0 -1,-0.2 1,-0.2 -2,-0.2 0.807 99.5 56.6 -68.0 -25.4 -70.2 -7.5 -10.9 81 86 A S T - 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