==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=9-NOV-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE/INHIBITOR 22-JUN-12 4FPL . COMPND 2 MOLECULE: SIALIDASE B; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOCOCCUS PNEUMONIAE; . AUTHOR P.BREAR . 658 1 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 25433.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 400 60.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 5 0.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 243 36.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 69 10.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 44 6.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 14 2.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 9 5 3 2 6 9 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 3 1 4 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 39 A I 0 0 123 0, 0.0 174,-0.0 0, 0.0 176,-0.0 0.000 360.0 360.0 360.0 164.3 38.7 -24.6 -26.5 2 40 A S - 0 0 95 1,-0.1 2,-0.1 2,-0.0 0, 0.0 -0.431 360.0 -95.0 -86.5 160.5 38.7 -24.1 -22.7 3 41 A P - 0 0 43 0, 0.0 170,-0.2 0, 0.0 3,-0.1 -0.429 12.9-146.6 -77.4 152.6 37.9 -20.9 -21.0 4 42 A I S S+ 0 0 44 168,-2.4 2,-0.3 1,-0.3 169,-0.2 0.599 92.0 20.3 -83.6 -16.7 40.5 -18.4 -19.9 5 43 A F E +A 172 0A 16 167,-1.2 167,-2.2 21,-0.0 2,-0.3 -0.959 65.2 172.7-155.5 136.6 38.3 -17.6 -16.9 6 44 A Q E +A 171 0A 128 -2,-0.3 2,-0.3 165,-0.2 165,-0.2 -0.982 12.1 150.0-147.7 130.5 35.4 -19.3 -15.2 7 45 A G E -A 170 0A 18 163,-2.3 163,-2.6 -2,-0.3 2,-0.3 -0.961 14.9-173.2-153.6 167.4 33.6 -18.4 -12.0 8 46 A G E +A 169 0A 29 -2,-0.3 161,-0.2 161,-0.2 160,-0.2 -0.963 47.4 48.4-163.0 175.9 30.2 -18.8 -10.5 9 47 A S E + 0 0 98 159,-1.3 2,-0.5 -2,-0.3 159,-0.2 0.951 59.8 165.6 43.1 63.7 27.5 -18.2 -7.8 10 48 A Y E -A 167 0A 38 157,-2.6 157,-2.7 -3,-0.1 2,-0.6 -0.921 26.6-149.1-101.3 126.5 27.9 -14.5 -7.7 11 49 A Q E -A 166 0A 110 -2,-0.5 2,-0.9 155,-0.2 155,-0.2 -0.861 3.5-157.0 -96.7 123.9 25.1 -12.6 -5.9 12 50 A L E +A 165 0A 1 153,-2.6 153,-2.5 -2,-0.6 2,-0.4 -0.826 23.7 163.3-100.9 95.7 24.5 -9.2 -7.3 13 51 A N E S-A 164 0A 83 -2,-0.9 151,-0.2 2,-0.5 -1,-0.1 -0.490 77.1 -68.1-113.8 62.5 22.8 -7.3 -4.5 14 52 A N S S+ 0 0 77 149,-2.2 2,-0.3 -2,-0.4 150,-0.1 0.854 116.5 97.2 56.1 38.8 23.2 -3.7 -5.6 15 53 A K S S- 0 0 148 148,-0.5 -2,-0.5 -4,-0.1 -1,-0.1 -0.861 72.6-128.5-155.8 115.9 27.0 -4.0 -5.0 16 54 A S - 0 0 31 -2,-0.3 2,-0.5 -4,-0.1 135,-0.2 -0.205 14.2-136.5 -67.2 157.0 29.4 -4.8 -7.8 17 55 A I E -F 150 0B 47 133,-2.5 133,-2.5 135,-0.1 2,-0.6 -0.975 13.4-138.3-116.4 125.2 32.0 -7.6 -7.6 18 56 A D E +F 149 0B 95 -2,-0.5 131,-0.2 131,-0.2 3,-0.1 -0.722 32.3 161.3 -87.4 119.0 35.6 -6.9 -8.7 19 57 A I > + 0 0 3 129,-2.5 4,-1.8 -2,-0.6 3,-0.3 0.082 37.0 118.3-119.2 21.0 37.1 -9.7 -10.7 20 58 A S H > S+ 0 0 13 128,-0.6 4,-2.3 1,-0.2 5,-0.4 0.906 74.1 51.6 -54.3 -47.7 39.9 -7.6 -12.3 21 59 A S H 4 S+ 0 0 95 1,-0.2 -1,-0.2 2,-0.2 4,-0.2 0.841 114.1 43.9 -62.1 -36.0 42.7 -9.7 -10.7 22 60 A L H 4 S+ 0 0 90 -3,-0.3 -1,-0.2 126,-0.1 -2,-0.2 0.780 128.4 22.1 -76.6 -28.4 41.2 -12.9 -12.0 23 61 A L H >X S+ 0 0 0 -4,-1.8 3,-1.7 -3,-0.3 4,-1.1 0.832 99.6 72.2-114.1 -41.6 40.4 -11.8 -15.5 24 62 A L G >< S+ 0 0 46 -4,-2.3 3,-0.6 1,-0.3 -3,-0.1 0.866 101.5 45.5 -58.3 -45.3 42.2 -8.8 -17.0 25 63 A D G 34 S+ 0 0 122 -5,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.518 108.0 61.9 -77.2 -3.3 45.7 -10.4 -17.5 26 64 A K G <4 S+ 0 0 86 -3,-1.7 -1,-0.2 2,-0.0 -2,-0.2 0.754 81.4 96.1 -86.3 -30.8 43.9 -13.4 -19.1 27 65 A L << + 0 0 21 -4,-1.1 2,-0.3 -3,-0.6 5,-0.1 -0.411 51.0 158.4 -65.7 127.9 42.3 -11.5 -21.9 28 66 A S + 0 0 71 -2,-0.2 3,-0.2 3,-0.2 2,-0.0 -0.992 35.2 20.0-154.7 147.8 44.4 -11.6 -25.1 29 67 A G S S- 0 0 56 -2,-0.3 111,-0.2 1,-0.2 146,-0.1 -0.162 86.8 -82.7 81.3 177.1 43.8 -11.1 -28.8 30 68 A E S S+ 0 0 100 144,-0.1 2,-0.4 109,-0.1 109,-0.3 0.459 102.1 75.0-102.4 -3.2 41.0 -9.2 -30.4 31 69 A S + 0 0 20 -3,-0.2 2,-0.3 82,-0.1 82,-0.2 -0.932 56.5 170.3-113.7 134.6 38.2 -11.9 -30.4 32 70 A Q E -B 112 0A 1 80,-2.0 80,-3.4 -2,-0.4 2,-0.4 -0.992 21.4-155.5-142.5 150.9 36.3 -12.9 -27.3 33 71 A T E -BC 111 173A 0 140,-2.1 140,-2.4 -2,-0.3 2,-0.5 -0.985 8.4-161.3-121.8 134.5 33.3 -14.9 -26.2 34 72 A V E -BC 110 172A 1 76,-2.8 76,-2.9 -2,-0.4 2,-0.5 -0.980 5.3-172.5-112.1 124.9 31.3 -14.4 -23.0 35 73 A V E +BC 109 171A 15 136,-2.7 136,-2.9 -2,-0.5 2,-0.4 -0.979 13.7 172.3-115.3 125.7 29.1 -17.2 -21.7 36 74 A M E -BC 108 170A 0 72,-3.0 72,-3.4 -2,-0.5 2,-0.6 -0.983 24.4-163.6-141.7 126.2 26.9 -16.3 -18.8 37 75 A K E +BC 107 169A 10 132,-2.7 132,-1.8 -2,-0.4 131,-1.4 -0.943 33.4 161.5-106.0 118.6 24.1 -18.0 -16.9 38 76 A F E -BC 106 167A 1 68,-2.1 68,-3.1 -2,-0.6 2,-0.4 -0.941 32.3-147.2-141.6 155.5 22.2 -15.5 -14.8 39 77 A K E + C 0 166A 64 127,-2.6 127,-2.6 -2,-0.3 2,-0.1 -0.955 17.2 167.6-120.2 143.2 18.9 -14.9 -13.0 40 78 A A + 0 0 1 64,-0.4 125,-0.1 -2,-0.4 64,-0.1 -0.593 12.8 152.6-153.6 88.7 17.2 -11.5 -12.5 41 79 A D S S+ 0 0 52 1,-0.2 124,-0.1 -2,-0.1 56,-0.1 0.474 84.2 33.0 -98.0 -4.9 13.7 -11.8 -11.2 42 80 A K S S+ 0 0 142 122,-0.3 -1,-0.2 55,-0.1 28,-0.1 -0.321 86.4 176.4-139.4 57.3 13.7 -8.4 -9.6 43 81 A P - 0 0 18 0, 0.0 122,-0.1 0, 0.0 2,-0.1 -0.205 31.5-115.6 -66.4 152.4 15.9 -6.3 -11.9 44 82 A N - 0 0 54 120,-0.2 3,-0.2 118,-0.1 120,-0.1 -0.359 42.9 -94.8 -67.8 165.5 16.6 -2.6 -11.5 45 83 A S S S+ 0 0 79 1,-0.3 25,-2.2 24,-0.2 2,-0.4 0.887 111.9 6.3 -51.5 -46.9 15.3 -0.4 -14.3 46 84 A L E S+G 69 0B 57 23,-0.2 2,-0.3 116,-0.2 -1,-0.3 -0.958 82.2 165.2-141.3 120.6 18.6 -0.5 -16.1 47 85 A Q E -G 68 0B 0 21,-2.6 21,-2.7 -2,-0.4 2,-0.4 -0.994 30.6-132.4-142.5 145.4 21.5 -2.6 -14.9 48 86 A A E -G 67 0B 0 113,-3.0 19,-0.3 -2,-0.3 115,-0.2 -0.785 11.1-172.3 -94.7 135.0 24.9 -4.0 -16.1 49 87 A L E S+ 0 0 2 17,-3.0 102,-1.8 -2,-0.4 2,-0.4 0.829 75.6 13.3 -87.1 -39.6 25.7 -7.6 -15.6 50 88 A F E -GH 66 150B 0 16,-1.4 16,-2.5 100,-0.3 2,-0.4 -0.999 67.6-175.1-144.8 139.6 29.3 -7.5 -16.7 51 89 A G E -GH 65 149B 0 98,-3.0 98,-3.3 -2,-0.4 2,-0.4 -0.997 18.5-173.0-140.3 141.1 31.7 -4.7 -17.3 52 90 A L E +GH 64 148B 0 12,-2.8 12,-3.4 -2,-0.4 2,-0.3 -0.994 37.1 162.0-122.3 125.2 35.2 -4.0 -18.6 53 91 A S E -GH 63 147B 0 94,-2.3 93,-2.7 -2,-0.4 94,-1.7 -0.978 52.7-121.7-147.2 153.8 36.2 -0.4 -18.0 54 92 A N - 0 0 3 8,-2.7 102,-0.5 5,-0.3 8,-0.3 -0.890 32.8-172.0 -83.9 109.2 38.8 2.3 -17.8 55 93 A S + 0 0 15 -2,-0.8 102,-0.3 89,-0.3 -1,-0.1 0.366 42.3 117.4 -88.0 6.4 37.9 3.5 -14.2 56 94 A K S > S- 0 0 93 88,-0.2 3,-2.0 100,-0.2 100,-0.3 -0.375 77.4 -87.2 -83.1 153.1 40.2 6.5 -14.4 57 95 A A T 3 S+ 0 0 85 1,-0.3 -1,-0.1 2,-0.1 -2,-0.0 -0.269 114.5 19.9 -52.5 132.6 39.2 10.2 -14.1 58 96 A G T 3 S+ 0 0 61 -3,-0.1 -1,-0.3 1,-0.0 3,-0.1 0.449 106.9 82.7 85.9 -0.2 38.4 11.6 -17.6 59 97 A F X + 0 0 65 -3,-2.0 3,-1.6 1,-0.1 -5,-0.3 -0.106 42.5 130.7-126.6 38.0 37.7 8.2 -19.2 60 98 A K T 3 S+ 0 0 103 1,-0.3 -1,-0.1 95,-0.1 -3,-0.1 0.637 72.8 56.6 -68.5 -15.0 34.1 7.6 -18.2 61 99 A N T 3 S+ 0 0 51 -3,-0.1 2,-0.5 1,-0.1 -1,-0.3 -0.015 83.5 93.7-107.0 27.4 33.2 6.7 -21.7 62 100 A N < + 0 0 0 -3,-1.6 -8,-2.7 -8,-0.3 2,-0.3 -0.814 62.1 100.5-119.6 87.8 35.8 4.0 -22.1 63 101 A Y E -GI 53 79B 1 16,-0.7 16,-2.7 -2,-0.5 2,-0.4 -0.994 56.2-136.7-162.0 157.7 34.1 0.7 -21.3 64 102 A F E +GI 52 78B 0 -12,-3.4 -12,-2.8 -2,-0.3 2,-0.3 -0.968 32.9 173.7-114.3 140.4 32.4 -2.5 -22.5 65 103 A S E -GI 51 77B 0 12,-2.1 12,-1.6 -2,-0.4 2,-0.5 -0.995 31.9-148.3-143.7 148.3 29.2 -3.6 -20.8 66 104 A I E +GI 50 76B 0 -16,-2.5 -17,-3.0 -2,-0.3 -16,-1.4 -0.993 39.0 174.3-107.9 120.2 26.5 -6.2 -21.1 67 105 A F E -GI 48 75B 8 8,-2.6 8,-2.5 -2,-0.5 2,-0.3 -0.852 20.6-163.3-125.6 164.8 23.4 -4.6 -19.7 68 106 A M E -GI 47 74B 0 -21,-2.7 -21,-2.6 -2,-0.3 2,-0.3 -0.935 10.9-141.5-138.0 160.2 19.7 -5.4 -19.4 69 107 A R E > -G 46 0B 93 4,-2.1 3,-2.1 -2,-0.3 -23,-0.2 -0.848 31.7-110.9-115.6 162.2 16.5 -3.3 -18.7 70 108 A D T 3 S+ 0 0 59 -25,-2.2 -24,-0.1 -2,-0.3 -25,-0.1 0.526 114.3 71.7 -71.0 -1.3 13.5 -4.2 -16.6 71 109 A S T 3 S- 0 0 33 -26,-0.3 541,-0.6 2,-0.1 22,-0.4 0.493 122.4-103.0 -82.6 -5.5 11.5 -4.5 -19.9 72 110 A G S < S+ 0 0 1 -3,-2.1 22,-2.7 1,-0.3 23,-1.0 0.474 75.0 145.6 92.9 3.2 13.5 -7.7 -20.6 73 111 A E - 0 0 15 20,-0.2 -4,-2.1 21,-0.2 2,-0.3 -0.509 30.4-162.5 -75.0 140.9 15.8 -5.9 -23.1 74 112 A I E +IJ 68 91B 0 17,-2.1 17,-2.6 -6,-0.2 2,-0.3 -0.922 20.9 142.0-120.5 149.2 19.4 -7.1 -23.4 75 113 A G E -IJ 67 90B 0 -8,-2.5 -8,-2.6 -2,-0.3 2,-0.3 -0.990 34.3-126.0-166.1 176.7 22.3 -5.3 -24.9 76 114 A V E -IJ 66 89B 0 13,-2.7 13,-2.1 -2,-0.3 2,-0.4 -0.955 8.7-155.9-129.7 155.0 26.0 -4.3 -25.0 77 115 A E E -IJ 65 88B 29 -12,-1.6 -12,-2.1 -2,-0.3 2,-0.3 -0.960 7.2-174.1-119.0 145.4 27.9 -1.0 -25.1 78 116 A I E +IJ 64 87B 0 9,-2.2 9,-2.7 -2,-0.4 2,-0.4 -0.890 18.8 171.1-141.1 106.7 31.4 -0.8 -26.5 79 117 A R E +IJ 63 86B 41 -16,-2.7 -16,-0.7 -2,-0.3 2,-0.3 -0.949 21.0 165.9-132.5 137.8 33.0 2.6 -26.1 80 118 A D E >> - J 0 85B 0 5,-2.7 5,-2.4 -2,-0.4 4,-0.7 -0.880 16.1-163.5-149.6 111.7 36.4 4.2 -26.5 81 119 A A T 45S+ 0 0 49 -2,-0.3 3,-0.3 1,-0.2 -1,-0.1 0.845 90.8 55.2 -68.3 -35.3 36.6 8.0 -26.6 82 120 A Q T 45S+ 0 0 101 1,-0.2 -1,-0.2 2,-0.1 -20,-0.0 0.907 113.3 42.2 -65.0 -39.7 40.1 8.1 -28.1 83 121 A K T 45S- 0 0 91 2,-0.1 -1,-0.2 60,-0.0 -2,-0.2 0.571 109.6-126.8 -79.6 -10.2 38.9 6.0 -31.0 84 122 A G T <5 + 0 0 60 -4,-0.7 2,-0.5 -3,-0.3 -3,-0.2 0.859 61.5 142.6 63.6 36.4 35.7 8.0 -31.2 85 123 A I E < +J 80 0B 12 -5,-2.4 -5,-2.7 2,-0.0 2,-0.3 -0.941 23.9 166.0-115.3 124.7 33.6 4.8 -31.0 86 124 A N E +J 79 0B 89 -2,-0.5 2,-0.4 -7,-0.2 -7,-0.2 -0.748 15.0 178.9-134.5 86.6 30.3 4.5 -29.1 87 125 A Y E +J 78 0B 44 -9,-2.7 -9,-2.2 -2,-0.3 2,-0.4 -0.713 6.9 177.3 -90.3 137.4 28.6 1.3 -30.1 88 126 A L E +J 77 0B 85 -2,-0.4 2,-0.4 -11,-0.2 -11,-0.2 -0.942 2.8 173.9-135.8 118.8 25.3 0.2 -28.6 89 127 A F E +J 76 0B 24 -13,-2.1 -13,-2.7 -2,-0.4 2,-0.3 -0.971 15.6 146.0-120.8 142.3 23.6 -2.9 -29.8 90 128 A S E -J 75 0B 37 -2,-0.4 -15,-0.3 -15,-0.3 -22,-0.1 -0.989 31.9-149.9-162.8 162.1 20.4 -4.3 -28.1 91 129 A R E -J 74 0B 12 -17,-2.6 -17,-2.1 -2,-0.3 3,-0.3 -0.943 21.6-130.1-144.2 130.6 17.1 -6.1 -28.5 92 130 A P S S+ 0 0 2 0, 0.0 -19,-0.1 0, 0.0 -20,-0.1 -0.131 86.2 24.0 -60.4 165.4 13.9 -5.7 -26.5 93 131 A A S S+ 0 0 13 -22,-0.4 -20,-0.2 1,-0.1 -21,-0.1 0.840 77.2 128.6 47.9 54.5 12.1 -8.7 -25.1 94 132 A S + 0 0 12 -22,-2.7 2,-0.3 -3,-0.3 -21,-0.2 0.463 53.4 72.5-112.6 -5.5 15.1 -11.1 -25.0 95 133 A L - 0 0 3 -23,-1.0 2,-0.4 -24,-0.1 28,-0.0 -0.744 59.2-144.9-118.3 154.9 14.9 -12.3 -21.4 96 134 A W + 0 0 2 -2,-0.3 -56,-0.3 1,-0.1 10,-0.1 -0.901 13.6 179.1-106.6 152.1 12.9 -14.5 -19.0 97 135 A G - 0 0 0 1,-0.4 7,-2.4 -2,-0.4 2,-0.3 0.773 62.2 -2.1-110.5 -58.9 12.4 -13.5 -15.3 98 136 A K E -K 103 0C 51 5,-0.3 -1,-0.4 508,-0.1 2,-0.3 -0.963 48.8-173.7-139.5 153.4 10.3 -16.1 -13.6 99 137 A H E > S-K 102 0C 28 3,-2.2 3,-2.0 -2,-0.3 508,-0.1 -0.956 81.8 -4.6-149.7 128.8 8.5 -19.3 -14.4 100 138 A K T 3 S- 0 0 82 -2,-0.3 3,-0.1 1,-0.3 506,-0.1 0.882 131.2 -59.1 50.2 40.4 6.2 -21.2 -12.0 101 139 A G T 3 S+ 0 0 51 504,-0.4 2,-0.4 1,-0.2 -1,-0.3 0.403 115.3 111.9 76.0 -3.1 7.3 -18.7 -9.4 102 140 A Q E < S-K 99 0C 147 -3,-2.0 -3,-2.2 1,-0.0 -1,-0.2 -0.860 76.2-103.0-109.3 141.3 11.0 -19.5 -9.7 103 141 A A E -K 98 0C 25 -2,-0.4 2,-0.3 -5,-0.2 -5,-0.3 -0.341 40.2-125.7 -59.1 128.8 13.7 -17.3 -11.1 104 142 A V - 0 0 9 -7,-2.4 2,-1.0 -2,-0.1 -64,-0.4 -0.599 9.0-131.1 -81.5 133.4 14.7 -18.4 -14.6 105 143 A E - 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