==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=30-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 21-JAN-06 2FS8 . COMPND 2 MOLECULE: TRYPTASE BETA-2; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR J.R.SOMOZA . 972 4 16 16 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 39530.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 597 61.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 310 31.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 23 2.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 77 7.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 111 11.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 56 5.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 1 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 20 11 8 15 9 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 4 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 16 A I 0 0 0 0, 0.0 2,-0.5 0, 0.0 3,-0.4 0.000 360.0 360.0 360.0 123.8 -4.4 32.0 65.2 2 17 A V B -A 188 0A 8 186,-3.2 186,-1.6 1,-0.2 132,-0.1 -0.859 360.0 -0.2-101.8 124.4 -2.4 34.7 66.9 3 18 A G S S+ 0 0 40 -2,-0.5 146,-0.2 130,-0.4 -1,-0.2 0.770 100.8 113.4 76.1 27.2 -2.2 38.2 65.4 4 19 A G - 0 0 28 -3,-0.4 2,-0.3 144,-0.1 144,-0.2 -0.142 57.4-121.3-107.8-151.9 -4.4 37.7 62.4 5 20 A Q E -B 147 0B 115 142,-1.5 142,-1.7 -2,-0.1 2,-0.1 -0.915 43.2 -74.7-146.5 164.8 -7.8 39.0 61.2 6 21 A E E -B 146 0B 94 -2,-0.3 140,-0.2 140,-0.2 139,-0.0 -0.411 57.0-117.8 -63.8 140.8 -11.0 37.3 60.3 7 22 A A - 0 0 2 138,-2.9 138,-0.2 56,-0.1 -1,-0.1 -0.588 24.7-108.0 -85.4 144.2 -10.6 35.6 56.9 8 23 A P > - 0 0 55 0, 0.0 3,-1.7 0, 0.0 -1,-0.1 -0.285 29.5-115.1 -64.3 152.4 -12.8 36.7 53.9 9 24 A R T 3 S+ 0 0 142 1,-0.3 -2,-0.0 4,-0.0 54,-0.0 0.790 112.3 59.0 -61.0 -24.9 -15.5 34.2 52.8 10 25 A S T 3 S+ 0 0 91 2,-0.0 2,-0.3 3,-0.0 -1,-0.3 0.441 93.6 83.2 -85.4 -0.4 -13.8 33.6 49.5 11 26 A K S < S+ 0 0 94 -3,-1.7 -4,-0.0 1,-0.2 95,-0.0 -0.754 82.1 20.3-109.8 155.2 -10.4 32.4 50.9 12 27 A W > + 0 0 9 -2,-0.3 3,-1.2 1,-0.1 -1,-0.2 0.942 58.1 173.6 60.3 55.8 -9.1 29.1 52.2 13 28 A P T 3 S+ 0 0 11 0, 0.0 48,-0.4 0, 0.0 95,-0.2 0.604 76.0 57.0 -65.5 -16.4 -11.7 26.8 50.4 14 29 A W T 3 S+ 0 0 15 93,-0.2 19,-1.9 95,-0.1 96,-0.1 0.539 77.7 115.8 -92.8 -12.0 -9.7 23.7 51.6 15 30 A Q E < +J 32 0C 0 -3,-1.2 46,-0.3 17,-0.2 2,-0.3 -0.384 42.5 179.1 -63.4 132.3 -9.8 24.5 55.4 16 31 A V E -J 31 0C 1 15,-1.9 15,-1.2 44,-0.2 2,-0.4 -0.854 22.8-136.0-130.9 168.9 -11.8 22.0 57.4 17 32 A S E -JK 30 59C 0 42,-2.6 42,-3.0 -2,-0.3 2,-0.5 -0.991 13.4-144.1-126.6 131.6 -12.7 21.4 61.0 18 33 A L E -JK 29 58C 0 11,-2.8 10,-1.9 -2,-0.4 11,-0.9 -0.831 19.3-162.0 -96.6 132.0 -12.5 18.0 62.8 19 34 A R E -JK 27 57C 0 38,-3.9 38,-2.9 -2,-0.5 2,-0.4 -0.939 8.3-155.7-120.1 140.1 -15.2 17.3 65.4 20 35 A V E -JK 26 56C 54 6,-2.2 6,-1.4 -2,-0.4 36,-0.2 -0.906 30.9-106.6-111.3 138.6 -15.4 14.8 68.2 21 36 A H + 0 0 113 34,-2.7 4,-0.1 -2,-0.4 34,-0.1 -0.333 65.9 109.8 -63.4 140.4 -18.7 13.6 69.6 22 37 A G S S- 0 0 33 -2,-0.0 3,-0.1 0, 0.0 -1,-0.0 -0.843 89.0 -25.1-176.6-148.9 -19.7 14.8 73.0 23 37AA P S S- 0 0 130 0, 0.0 2,-0.3 0, 0.0 -2,-0.1 0.770 139.3 -9.9 -54.2 -24.0 -22.3 17.2 74.5 24 37BA Y S S- 0 0 72 -4,-0.2 2,-1.7 360,-0.0 -4,-0.1 -0.935 98.1 -76.1-161.9 165.5 -22.1 18.6 70.9 25 38 A W - 0 0 49 -2,-0.3 -4,-0.2 -4,-0.1 2,-0.2 -0.565 61.3-163.7 -76.6 90.9 -19.8 18.1 68.0 26 39 A M E -J 20 0C 81 -2,-1.7 -6,-2.2 -6,-1.4 2,-0.4 -0.488 19.0-119.3 -82.5 143.6 -16.8 20.1 69.2 27 40 A H E +J 19 0C 21 -8,-0.2 -8,-0.2 -2,-0.2 3,-0.1 -0.659 39.9 158.8 -79.3 125.7 -13.9 21.3 67.2 28 41 A F E + 0 0 59 -10,-1.9 2,-0.3 -2,-0.4 164,-0.2 0.591 62.0 6.5-120.8 -21.3 -10.6 19.9 68.4 29 42 A a E -J 18 0C 6 -11,-0.9 -11,-2.8 164,-0.1 -1,-0.3 -0.978 64.2-115.3-155.6 167.9 -8.3 20.2 65.3 30 43 A G E +J 17 0C 1 164,-2.8 12,-0.4 -2,-0.3 2,-0.3 -0.433 29.3 170.0-100.7 175.5 -7.9 21.6 61.8 31 44 A G E -J 16 0C 0 -15,-1.2 -15,-1.9 -2,-0.1 2,-0.3 -0.946 22.1-126.6-166.2-177.3 -7.5 19.9 58.4 32 45 A S E -JL 15 40C 0 8,-1.6 8,-2.7 -2,-0.3 2,-0.7 -0.995 19.7-122.9-149.1 142.1 -7.4 20.4 54.7 33 46 A L E + L 0 39C 2 -19,-1.9 77,-2.1 -2,-0.3 6,-0.2 -0.764 33.5 165.6 -86.6 117.6 -9.2 18.8 51.7 34 47 A I E S+ 0 0 17 4,-1.6 5,-0.2 -2,-0.7 -1,-0.2 0.416 71.1 10.8-108.5 -2.4 -6.6 17.4 49.3 35 48 A H E > S- L 0 38C 47 3,-1.2 3,-1.7 73,-0.1 -1,-0.4 -0.937 87.5 -96.8-169.5 149.6 -9.0 15.3 47.3 36 49 A P T 3 S+ 0 0 44 0, 0.0 65,-2.8 0, 0.0 63,-0.1 0.685 127.4 29.1 -48.2 -21.1 -12.8 14.9 47.1 37 50 A Q T 3 S+ 0 0 51 63,-0.2 60,-2.7 61,-0.1 2,-0.4 0.379 112.2 75.4-122.5 4.7 -12.5 12.0 49.5 38 51 A W E < -LM 35 96C 13 -3,-1.7 -4,-1.6 58,-0.2 -3,-1.2 -0.941 48.4-169.0-128.2 145.8 -9.4 12.8 51.6 39 52 A V E -LM 33 95C 0 56,-2.1 56,-2.7 -2,-0.4 2,-0.4 -0.987 13.9-148.0-127.1 134.7 -8.5 15.2 54.4 40 53 A L E +LM 32 94C 0 -8,-2.7 -8,-1.6 -2,-0.4 2,-0.3 -0.857 32.6 146.0-104.2 135.0 -4.9 15.8 55.6 41 54 A T E - M 0 93C 0 52,-2.2 52,-2.1 -2,-0.4 2,-0.3 -0.853 50.0 -65.9-151.8-175.9 -4.3 16.6 59.2 42 55 A A >> - 0 0 0 -12,-0.4 3,-1.0 -2,-0.3 4,-0.6 -0.625 33.4-136.2 -83.3 139.1 -1.8 16.2 62.1 43 56 A A H >> S+ 0 0 0 -2,-0.3 4,-1.2 1,-0.2 3,-0.8 0.848 103.8 59.9 -63.3 -31.0 -1.2 12.7 63.3 44 57 A H H 34 S+ 0 0 61 1,-0.2 -1,-0.2 2,-0.2 169,-0.0 0.734 93.8 66.5 -72.7 -16.7 -1.3 13.9 67.0 45 58 A a H <4 S+ 0 0 13 -3,-1.0 -1,-0.2 1,-0.2 -2,-0.2 0.829 120.2 17.5 -68.6 -33.2 -4.9 15.1 66.4 46 59 A V H << S- 0 0 5 -3,-0.8 5,-0.2 -4,-0.6 -2,-0.2 0.308 117.9-109.6-123.0 7.3 -6.2 11.5 65.9 47 60 A G < - 0 0 9 -4,-1.2 37,-0.1 -5,-0.1 3,-0.1 -0.753 31.3-111.9-146.1-166.5 -3.4 9.4 67.4 48 60AA P S S+ 0 0 21 0, 0.0 851,-3.1 0, 0.0 852,-0.4 -0.996 110.4 58.1 -71.2 -16.5 -1.1 7.6 68.4 49 60BA D B S-P 898 0D 32 849,-0.2 849,-0.3 850,-0.1 848,-0.1 -0.570 95.8-104.1 -82.7 141.2 -3.9 5.6 69.9 50 60CA V - 0 0 79 847,-2.0 2,-0.3 -2,-0.2 -1,-0.1 -0.326 40.5-154.9 -62.2 137.9 -6.7 4.1 67.8 51 60DA K - 0 0 99 -5,-0.2 2,-0.8 -2,-0.0 26,-0.1 -0.776 25.6 -98.3-117.6 159.3 -10.0 5.9 68.0 52 60EA D > - 0 0 114 -2,-0.3 3,-1.2 1,-0.2 4,-0.1 -0.661 31.5-154.6 -75.0 110.9 -13.7 5.1 67.5 53 61 A L G > S+ 0 0 59 -2,-0.8 3,-2.5 1,-0.3 21,-0.3 0.860 89.7 63.5 -54.4 -41.3 -14.3 6.3 64.0 54 62 A A G 3 S+ 0 0 68 1,-0.3 -1,-0.3 19,-0.1 -2,-0.1 0.795 96.2 59.8 -56.0 -29.7 -18.0 6.8 64.7 55 63 A A G < S+ 0 0 33 -3,-1.2 -34,-2.7 -35,-0.1 2,-0.4 0.414 96.5 81.3 -80.9 3.4 -17.1 9.4 67.3 56 64 A L E < +K 20 0C 16 -3,-2.5 18,-0.6 -36,-0.2 2,-0.3 -0.905 53.1 173.9-117.0 140.8 -15.4 11.5 64.6 57 65 A R E -KN 19 73C 72 -38,-2.9 -38,-3.9 -2,-0.4 2,-0.4 -0.981 19.1-140.3-143.0 154.1 -16.8 13.9 62.1 58 66 A V E -KN 18 72C 0 14,-2.6 14,-3.3 -2,-0.3 2,-0.5 -0.940 8.5-161.7-119.0 134.8 -15.5 16.4 59.5 59 67 A Q E -K 17 0C 8 -42,-3.0 -42,-2.6 -2,-0.4 3,-0.1 -0.969 16.1-137.2-117.4 125.4 -16.9 19.8 58.7 60 68 A L - 0 0 30 -2,-0.5 -44,-0.2 10,-0.3 10,-0.1 -0.173 58.2 -40.4 -72.5 171.5 -16.0 21.6 55.4 61 69 A R + 0 0 11 -48,-0.4 2,-0.3 -46,-0.3 -1,-0.2 0.018 67.1 178.3 -38.8 130.7 -15.1 25.3 55.2 62 70 A E - 0 0 7 -3,-0.1 3,-0.2 1,-0.1 83,-0.1 -0.993 32.1-145.4-137.9 138.8 -17.3 27.3 57.5 63 71 A Q S S+ 0 0 9 -2,-0.3 82,-1.6 1,-0.3 2,-0.4 0.885 95.0 23.7 -74.0 -35.8 -17.2 31.1 58.0 64 72 A H B >> S-Q 144 0E 35 80,-0.2 3,-1.8 -3,-0.1 4,-0.6 -0.952 93.1-111.2-134.0 116.5 -18.2 30.6 61.7 65 73 A L T 34 S+ 0 0 4 78,-2.2 4,-0.0 -2,-0.4 -2,-0.0 -0.060 97.2 0.4 -44.2 135.6 -17.7 27.4 63.7 66 74 A Y T >4 S+ 0 0 17 1,-0.1 3,-0.6 319,-0.1 -1,-0.2 0.193 104.8 102.5 72.7 -17.7 -20.9 25.6 64.6 67 75 A Y T <4 S+ 0 0 64 -3,-1.8 -2,-0.1 75,-0.2 -1,-0.1 0.945 94.6 15.0 -62.0 -54.6 -23.1 28.1 62.8 68 79 A Q T 3< S+ 0 0 119 -4,-0.6 -1,-0.2 2,-0.0 -3,-0.1 -0.336 85.6 179.7-119.9 52.4 -23.9 26.2 59.6 69 80 A D < + 0 0 58 -3,-0.6 -10,-0.1 1,-0.1 2,-0.0 -0.237 21.3 163.3 -63.1 133.9 -22.9 22.6 60.4 70 81 A Q - 0 0 159 -10,-0.1 -10,-0.3 -12,-0.0 -1,-0.1 -0.573 31.0-156.8-143.3 68.1 -23.3 19.8 57.8 71 82 A L - 0 0 41 -12,-0.1 -12,-0.2 1,-0.1 -2,-0.0 -0.190 3.3-148.9 -57.3 138.7 -21.0 17.2 59.4 72 83 A L E -N 58 0C 50 -14,-3.3 -14,-2.6 26,-0.0 -1,-0.1 -0.928 14.5-127.3-112.9 128.3 -19.5 14.4 57.2 73 84 A P E -N 57 0C 45 0, 0.0 25,-1.6 0, 0.0 2,-0.4 -0.280 19.9-131.7 -70.8 158.7 -18.8 10.9 58.6 74 85 A V E -O 97 0C 6 -18,-0.6 23,-0.2 -21,-0.3 -18,-0.1 -0.912 18.5-174.2-117.1 137.7 -15.3 9.2 58.2 75 86 A S E S+ 0 0 48 21,-2.7 2,-0.3 -2,-0.4 22,-0.2 0.753 71.6 23.7-100.0 -29.0 -14.5 5.7 57.0 76 87 A R E -O 96 0C 119 20,-1.3 20,-2.3 -23,-0.1 2,-0.5 -0.998 51.7-160.0-142.0 145.9 -10.8 5.5 57.6 77 88 A I E -O 95 0C 21 -2,-0.3 2,-0.6 18,-0.2 18,-0.2 -0.992 17.0-173.0-119.6 115.6 -8.1 7.1 59.9 78 89 A I E -O 94 0C 9 16,-3.6 16,-3.8 -2,-0.5 2,-0.4 -0.930 3.3-175.7-117.9 111.6 -4.6 6.6 58.4 79 90 A V E -O 93 0C 11 -2,-0.6 14,-0.2 14,-0.2 12,-0.1 -0.874 38.2 -99.8-108.0 136.1 -1.8 7.8 60.7 80 91 A H > - 0 0 20 12,-2.1 3,-2.6 -2,-0.4 12,-0.1 -0.307 30.0-136.8 -55.5 120.1 1.8 7.7 59.6 81 92 A P T 3 S+ 0 0 54 0, 0.0 820,-3.2 0, 0.0 -1,-0.2 0.698 100.6 63.0 -52.4 -23.2 3.3 4.6 61.2 82 93 A Q T 3 S+ 0 0 109 818,-0.2 2,-0.2 8,-0.1 818,-0.1 0.539 85.4 106.3 -81.1 -4.6 6.4 6.5 62.3 83 94 A F < + 0 0 3 -3,-2.6 7,-0.1 7,-0.2 3,-0.1 -0.554 18.5 131.2 -84.5 141.5 4.4 8.7 64.5 84 95 A Y S S+ 0 0 25 5,-0.5 2,-0.3 1,-0.5 -1,-0.2 0.350 78.5 9.4-147.3 -50.9 4.2 8.7 68.3 85 96 A T >> - 0 0 17 4,-0.3 4,-1.8 1,-0.1 3,-0.8 -0.979 67.7-125.0-136.5 150.0 4.7 12.3 69.6 86 97 A A T 34 S+ 0 0 13 -2,-0.3 5,-0.3 1,-0.2 128,-0.1 0.853 109.8 57.4 -62.4 -35.8 4.8 15.5 67.6 87 98 A Q T 34 S+ 0 0 57 1,-0.2 -1,-0.2 2,-0.1 89,-0.2 0.759 106.7 49.6 -67.5 -25.4 8.2 16.5 68.9 88 99 A I T <4 S- 0 0 29 -3,-0.8 -1,-0.2 1,-0.2 2,-0.2 0.892 113.3-127.3 -80.6 -41.1 9.7 13.3 67.6 89 100 A G S < S+ 0 0 5 -4,-1.8 -5,-0.5 1,-0.1 -4,-0.3 -0.670 78.8 100.4 126.9-179.9 8.2 13.8 64.1 90 101 A A + 0 0 3 -2,-0.2 2,-2.6 88,-0.2 -10,-0.2 0.801 46.7 168.9 66.3 31.3 6.0 12.2 61.4 91 102 A D + 0 0 2 -5,-0.3 2,-0.3 -48,-0.1 137,-0.2 -0.434 32.7 113.5 -77.0 69.9 3.1 14.3 62.7 92 103 A I + 0 0 0 -2,-2.6 -12,-2.1 -50,-0.3 2,-0.3 -0.984 36.4 169.6-143.0 147.6 0.9 13.6 59.6 93 104 A A E -MO 41 79C 0 -52,-2.1 -52,-2.2 -2,-0.3 2,-0.4 -0.995 21.1-135.3-157.3 159.5 -2.3 11.7 59.0 94 105 A L E -MO 40 78C 0 -16,-3.8 -16,-3.6 -2,-0.3 2,-0.5 -0.955 7.3-162.0-124.5 138.7 -5.1 11.0 56.5 95 106 A L E -MO 39 77C 0 -56,-2.7 -56,-2.1 -2,-0.4 2,-0.6 -0.973 9.6-154.5-118.0 123.8 -8.9 10.9 56.9 96 107 A E E -MO 38 76C 39 -20,-2.3 -21,-2.7 -2,-0.5 -20,-1.3 -0.867 15.4-138.0-100.4 127.9 -10.9 9.1 54.2 97 108 A L E - 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